lacZ:On One Page

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Quickview | Gene | Product(s) | Expression| Evolution | References |

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Standard Name

lacZ

Gene Synonym(s)

ECK0341, b0344, JW0335[1], JW0335

Product Desc.

beta-galactosidase[2][3];

Component of β-galactosidase[2][3]

beta-D-Galactosidase[4]

Product Synonyms(s)

beta-D-galactosidase[1], B0344[2][1], LacZ[2][1], beta-D-galactosidase[2][1], lactase[2][1], beta-D-galactoside galactohydrolase[2][1] , ECK0341, JW0335, b0344

Function from GO

<GO_nr />

Knock-Out Phenotype

unable to grow on lactose as a carbon source

Regulation/Expression

transcription unit(s): lacZYA[2], lacZ, OP00045, lacAYZ

Regulation/Activity
Quick Links

porteco.png EcoCyc.gif regulondb.jpg

dnadisplay.png proteindisplay.png   pubmed.jpg   textpresso.jpg  

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Notes

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Repressed during biofilm formation. LacZ has an Ig-like beta-sandwich domain (residues 221-334 align to PFAM: PF00703). ebgA, uidA and lacZ are paralogs. Binds TrxA (Kumar, 2004)[5].[4]


Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

lacZ

Mnemonic

Lactose

Synonyms

ECK0341, b0344, JW0335[1], JW0335

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

7.81 minutes 

MG1655: 365529..362455
<gbrowseImage> name=NC_000913:362455..365529 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 338861..335787
<gbrowseImage> name=NC_012967:335787..338861 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 4175805..4179047
<gbrowseImage> name=NC_012759:4175805..4179047 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 365529..362455
<gbrowseImage> name=NC_007779:362455..365529 source=W3110 preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

362458

Edman degradation

PMID:97295
PMID:97298


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

lacZD202F

D202F

Obliterates all binding and catalysis

seeded from UniProt:P00722

lacZD202E,N

D202E,N

Causes a significant decrease in binding affinity in the absence of monovalent cations or in the presence of potassium ions, but only a moderate decrease in the presence of sodium ions

seeded from UniProt:P00722

lacZW1000F,G,L,T

W1000F,G,L,T

Decreases affinity for substrate

seeded from UniProt:P00722

lacZF602A

F602A

Decreases the stability of the loop 794-804

seeded from UniProt:P00722

lacZG795A

G795A

It forces the apoenzyme to adopt the closed rather than the open conformation. Reduces the binding affinity

seeded from UniProt:P00722

lacZE798A,L

E798A,L

The catalytic efficiency is not increased, when the sodium concentration increases

seeded from UniProt:P00722

lacZE798D,Q

E798D,Q

Small increase of the catalytic efficiency, when the sodium concentration increases

seeded from UniProt:P00722

lacZH541E,F,N

H541E,F,N

Poorly reactive with galactosyl substrates. Less stable to heat than wild-type

seeded from UniProt:P00722

lacZE538Q

E538Q

10000-fold decrease in the beta- galactosidase activity

seeded from UniProt:P00722

lacZE462H

E462H

Slowly inactivates galactosidase activity by reducing the binding of magnesium. It increases binding specificity

seeded from UniProt:P00722

lacZH358D,F,L,N

H358D,F,L,N

Less stable to heat than wild-type. Causes significant destabilizations of the first transition state

seeded from UniProt:P00722

lacZH392E,F,K

H392E,F,K

Essentially inactive unless very rapid purification. Causes very large destabilizations of the transition state

seeded from UniProt:P00722

lacZ4

CGSC:4649

lacZ43(Fs)

CGSC:4545

frameshift mutation

lacZ53(Am)

CGSC:4692

amber (UAG) mutation

lacZ36

CGSC:5084

lacZ125(Am)

PMID:5327654

CGSC:5303

amber (UAG) mutation

lacZ73

CGSC:5701

lacZ46

CGSC:5741

lacZ827(UGA)

CGSC:5781

opal (UGA) mutation

lacZ82(Am)

CGSC:5996

amber (UAG) mutation

lacZ39(del)

CGSC:6051

lacZ13(Oc)

CGSC:6357

ochre (UAA) mutation

lacZ90(Oc)

PMID:6790520 PMID:5327654

CGSC:6678

ochre (UAA) mutation

lacZ625(Am)

PMID:5327654

CGSC:6682

amber (UAG) mutation

lacZ118(Oc)

PMID:5327654 PMID:412841 PMID:4876923

CGSC:6685

ochre (UAA) mutation

lacZ659(Oc)

PMID:5327654

CGSC:6687

ochre (UAA) mutation

lacZ404(Oc)

PMID:5327654

CGSC:6690

ochre (UAA) mutation

lacZ521(UGA)

PMID:4915862

CGSC:6930

opal (UGA) mutation

lacZ813(UGA)

PMID:4915862

CGSC:6935

opal (UGA) mutation

lacZ105(Am)

CGSC:7341

amber (UAG) mutation

lacZ178

CGSC:7401

lacZ281(Am)

CGSC:7951

amber (UAG) mutation

lacZ57(del)

CGSC:7985

lacZ62

CGSC:8000

lacZ608(Am)

PMID:5327654 PMID:4915862

CGSC:8396

amber (UAG) mutation

lacZ84

CGSC:8606

lacZ75(Fs)

CGSC:8609

frameshift mutation

lacZ482(Am)

CGSC:8713

amber (UAG) mutation

lacZ58(del)(M15)

CGSC:8749

lacZ98::Tn10

CGSC:8864

lacZ545(Am)

CGSC:8947

amber (UAG) mutation

lacZ2286(Am)

CGSC:9797

amber (UAG) mutation

lacZ624(Am)

PMID:4915862

CGSC:10716

amber (UAG) mutation

lacZ95(del)

CGSC:10738

lacZ114((delH))

CGSC:10830

lacZ55

CGSC:11758

lacZ2210(Am)

CGSC:11841

amber (UAG) mutation

lacZ332(Fs)

PMID:1092652

CGSC:12130

lacZ4503(Am)

CGSC:12975

amber (UAG) mutation

lacZ49

CGSC:16030

lacZ56

CGSC:16066

lacZ131

CGSC:19612

lacZ2246

CGSC:19613

lacZ8(Am)

PMID:2141650

CGSC:23460

amber (UAG) mutation

lacZ59

CGSC:26947

lacZ4502::Tn10

PMID:337110

CGSC:59813

lacZ4787(del)(::rrnB-3)

PMID:10829079

CGSC:64656

lacZ4796::Tn5

CGSC:76213

lacZ536(Am)

CGSC:83183

amber (UAG) mutation

lacZ571(Am)

PMID:2501784

CGSC:83642

amber (UAG) mutation

lacZ8305::Mu cts62

PMID:1111215

CGSC:84395

lacZ4525::Tn10kan

PMID:9139905

CGSC:85232

lacZ572

PMID:2501784

CGSC:89114

lacZ574

PMID:2501784

CGSC:89115

lacZ573

PMID:2501784

CGSC:89116

lacZ575

PMID:2501784

CGSC:89118

lacZ576

PMID:2501784

CGSC:89119

lacZ1125

PMID:4915862 PMID:5327654

CGSC:89575


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW0335

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCACCATGATTACGGATTCACT

Primer 2:CCTTTTTGACACCAGACCAACTG

10A6

Kohara Phage

Genobase

PMID:3038334

proA81::Tn10

Linked marker

CAG18447 = CGSC7330[6]

est. P1 cotransduction: % [7]

lacI3042::Tn10

Linked marker

CAG18439 = CGSC7334[6]

est. P1 cotransduction: 87% [7]
Synonyms:lacI42::Tn10 nnnCAG18439 also carries lacZ118(0c) (CGSC).

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10527

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10527

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000520

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:945006

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0522

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0001183

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

LacZ

Synonyms

beta-D-galactosidase[1], B0344[2][1], LacZ[2][1], beta-D-galactosidase[2][1], lactase[2][1], beta-D-galactoside galactohydrolase[2][1] , ECK0341, JW0335, b0344

Product description

beta-galactosidase[2][3];

Component of β-galactosidase[2][3]

beta-D-Galactosidase[4]

EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status

GO:0000287

magnesium ion binding

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_01687

F

Seeded from EcoCyc (v14.0)

complete

GO:0000287

magnesium ion binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0460

F

Seeded from EcoCyc (v14.0)

complete

GO:0004565

beta-galactosidase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004199

F

Seeded from EcoCyc (v14.0)

complete

GO:0004565

beta-galactosidase activity

GOA:spec
GO_REF:0000003

IEA: Inferred from Electronic Annotation

EC:3.2.1.23

F

Seeded from EcoCyc (v14.0)

complete

GO:0009341

beta-galactosidase complex

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004199

C

Seeded from EcoCyc (v14.0)

complete

GO:0030145

manganese ion binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0464

F

Seeded from EcoCyc (v14.0)

complete

GO:0030246

carbohydrate binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR011013

F

Seeded from EcoCyc (v14.0)

complete

GO:0030246

carbohydrate binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR014718

F

Seeded from EcoCyc (v14.0)

complete

GO:0031402

sodium ion binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0915

F

Seeded from EcoCyc (v14.0)

complete

GO:0043169

cation binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR013781

F

Seeded from EcoCyc (v14.0)

complete

GO:0046872

metal ion binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0479

F

Seeded from EcoCyc (v14.0)

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

Subunits of β-galactosidase

could be indirect

Protein

yifB

PMID:16606699

Experiment(s):EBI-1136305

Protein

napC

PMID:16606699

Experiment(s):EBI-1136305

Protein

secA

PMID:16606699

Experiment(s):EBI-1136305
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MTMITDSLAV VLQRRDWENP GVTQLNRLAA HPPFASWRNS EEARTDRPSQ QLRSLNGEWR
FAWFPAPEAV PESWLECDLP EADTVVVPSN WQMHGYDAPI YTNVTYPITV NPPFVPTENP
TGCYSLTFNV DESWLQEGQT RIIFDGVNSA FHLWCNGRWV GYGQDSRLPS EFDLSAFLRA
GENRLAVMVL RWSDGSYLED QDMWRMSGIF RDVSLLHKPT TQISDFHVAT RFNDDFSRAV
LEAEVQMCGE LRDYLRVTVS LWQGETQVAS GTAPFGGEII DERGGYADRV TLRLNVENPK
LWSAEIPNLY RAVVELHTAD GTLIEAEACD VGFREVRIEN GLLLLNGKPL LIRGVNRHEH
HPLHGQVMDE QTMVQDILLM KQNNFNAVRC SHYPNHPLWY TLCDRYGLYV VDEANIETHG
MVPMNRLTDD PRWLPAMSER VTRMVQRDRN HPSVIIWSLG NESGHGANHD ALYRWIKSVD
PSRPVQYEGG GADTTATDII CPMYARVDED QPFPAVPKWS IKKWLSLPGE TRPLILCEYA
HAMGNSLGGF AKYWQAFRQY PRLQGGFVWD WVDQSLIKYD ENGNPWSAYG GDFGDTPNDR
QFCMNGLVFA DRTPHPALTE AKHQQQFFQF RLSGQTIEVT SEYLFRHSDN ELLHWMVALD
GKPLASGEVP LDVAPQGKQL IELPELPQPE SAGQLWLTVR VVQPNATAWS EAGHISAWQQ
WRLAENLSVT LPAASHAIPH LTTSEMDFCI ELGNKRWQFN RQSGFLSQMW IGDKKQLLTP
LRDQFTRAPL DNDIGVSEAT RIDPNAWVER WKAAGHYQAE AALLQCTADT LADAVLITTA
HAWQHQGKTL FISRKTYRID GSGQMAITVD VEVASDTPHP ARIGLNCQLA QVAERVNWLG
LGPQENYPDR LTAACFDRWD LPLSDMYTPY VFPSENGLRC GTRELNYGPH QWRGDFQFNI
SRYSQQQLME TSHRHLLHAE EGTWLNIDGF HMGIGGDDSW SPSVSAEFQL SAGRYHYQLV WCQK
Length

1,024

Mol. Wt

116.482 kDa

pI

5.3 (calculated)

Extinction coefficient

260,690 - 262,690 (calc based on 31 Y, 39 W, and 16 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Initiator Methionine

1

Removed

UniProt:P00722

Domain

749..1022

PF02929 Beta galactosidase small chain

PMID:19920124

Domain

221..334

PF00703 domain

PMID:19920124

Domain

336..630

PF02836 Glycosyl hydrolases family 2, TIM barrel domain

PMID:19920124

Domain

49..219

PF02837 Glycosyl hydrolases family 2, sugar binding domain

PMID:19920124

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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=lacZ taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

See Help:Jmol in EcoliWiki

E. COLI BETA-GALACTOSIDASE AT 1.7 ANGSTROM courtesy of the PDB.
<jmol> <jmolApplet> <color>white</color> <uploadedFileContents >1DP0.pdb</uploadedFileContents> <name></name> <script> wireframe off; spacefill off; trace off; rotate ON; cartoon; color chain; </script> </jmolApplet> </jmol>
<jmol> <jmolCheckbox > <scriptWhenChecked > rotate on; </scriptWhenChecked > <scriptWhenUnchecked > rotate off; </scriptWhenUnchecked > <checked>true</checked> <text>rotate</text> </jmolCheckbox > <jmolButton><script>reset; </script><text>reset</text></jmolButton> </jmol>



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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16128329

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:945006

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0001183

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P00722

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10527

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10527

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:945006

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000520

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0522

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)
  • Medium:
  • Temperature (°C):
  • Doubling time (min): Growth Rate(min):
  • Growth phase: other:

Protein

E. coli K-12 MG1655

31

molecules/cell/generation

  • Medium: MOPS Complete
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

10

molecules/cell/generation

  • Medium: MOPS Minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

8a

molecules/cell/generation

  • Medium: MOPS Complete without Methionine
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

Low confidence in the sequencing data set.

PMID: 24766808

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Notes

Transcription and Transcriptional Regulation

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<protect>

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

lacZYA

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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:365509..365549 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GEO Profiles:b0344 (EcoliWiki Page)

NCBI GEO profiles for lacZ

microarray

GenExpDB:b0344 (EcoliWiki Page)

Summary of data for lacZ from multiple microarray studies

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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes

GFP Fusion

Intergenic region (365438..365669) fused to gfpmut2.

OpenBioSystems

GFP fusion described in Zaslaver, et al.
Plate:109-AZ02, 109-AZ20; Well:C8[8]

GFP Fusion

Intergenic region (365438..365669) fused to gfpmut2.

OpenBioSystems

Raw protomer fusion data from Zaslaver, et al.
Plate:109-AZ01..109-AZ20; Well:H1[8]
Each of the plates in the experiment contained a well with a lacZ fusion for normalization.


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Notes

Accessions Related to lacZ Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10527

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0522

Escherichia coli str. K-12 substr. MG1655

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b0344

EcoGene

EcoGene:EG10527

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000520

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0001183

Escherichia coli str. K-12 substr. MG1655

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Notes

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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism Homologs (Statistics) Comments

Arabidopsis thaliana

  • AT3G54440 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Oryza gramene

  • Q5JKZ9 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

E. coli O157

LACZ

From ECOO157CYC

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Do-It-Yourself Web Tools

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Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

Pfam (EcoliWiki Page)

PF00703 domain

Pfam (EcoliWiki Page)

PF02929 Beta galactosidase small chain

Pfam (EcoliWiki Page)

PF02836 Glycosyl hydrolases family 2, TIM barrel domain

Pfam (EcoliWiki Page)

PF02837 Glycosyl hydrolases family 2, sugar binding domain

Superfamily (EcoliWiki Page)

SUPERFAMILY:49303

Superfamily (EcoliWiki Page)

SUPERFAMILY:49785

Superfamily (EcoliWiki Page)

SUPERFAMILY:51445

Panther (EcoliWiki Page)

PTHR10066:SF41

Superfamily (EcoliWiki Page)

SUPERFAMILY:74650

EcoCyc

EcoCyc:EG10527

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10527

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000520

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0522

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0001183

Escherichia coli str. K-12 substr. MG1655

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Notes

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References

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  1. ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 2.13 2.14 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 3.2 3.3 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ 4.0 4.1 4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ Kumar, JK et al. (2004) Proteomic analysis of thioredoxin-targeted proteins in Escherichia coli. Proc. Natl. Acad. Sci. U.S.A. 101 3759-64 PubMed
  6. ↑ 6.0 6.1 CGSC: The Coli Genetics Stock Center
  7. ↑ 7.0 7.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  8. ↑ 8.0 8.1 Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

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