From EcoliWiki
Jump to: navigation, search

Nichols, BP, Shafiq, O and Meiners, V (1998) Sequence analysis of Tn10 insertion sites in a collection of Escherichia coli strains used for genetic mapping and strain construction. J. Bacteriol. 180:6408-11


The chromosomal insertion sites of Tn10-containing Escherichia coli strains were amplified by inverse PCR, and the nucleotide sequences of the junctions were determined. In 95 strains analyzed, 88 unique Tn10 positions were determined and matched to the E. coli chromosome sequence. Two gaps in insertion site positions were noted, one including the terminus of DNA replication and another bounded by recombination hot spots RhsA and RhsB.


PubMed PMC107733


Base Sequence; Binding Sites/genetics; Chromosome Mapping/methods; Chromosomes, Bacterial/genetics; DNA Primers/genetics; DNA Transposable Elements/genetics; DNA, Bacterial/genetics; Escherichia coli/genetics; Genes, Bacterial; Open Reading Frames; Polymerase Chain Reaction


You can help EcoliWiki by summarizing why this paper is useful

Useful Materials and Methods

You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.


Add GO annotations

EcoliWiki Links

Add links to pages that link here (e.g. gene, product, method pages)


See Help:References for how to manage references in EcoliWiki.