lacZ:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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Standard name

lacZ

Mnemonic

Lactose

Synonyms

ECK0341, b0344, JW0335[1], JW0335

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Notes

Location(s) and DNA Sequence

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Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

7.81 minutes 

MG1655: 365529..362455
<gbrowseImage> name=NC_000913:362455..365529 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 338861..335787
<gbrowseImage> name=NC_012967:335787..338861 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 4175805..4179047
<gbrowseImage> name=NC_012759:4175805..4179047 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 365529..362455
<gbrowseImage> name=NC_007779:362455..365529 source=W3110 preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

362458

Edman degradation

PMID:97295[2]
PMID:97298[3]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

lacZD202F

D202F

Obliterates all binding and catalysis

seeded from UniProt:P00722

lacZD202E,N

D202E,N

Causes a significant decrease in binding affinity in the absence of monovalent cations or in the presence of potassium ions, but only a moderate decrease in the presence of sodium ions

seeded from UniProt:P00722

lacZW1000F,G,L,T

W1000F,G,L,T

Decreases affinity for substrate

seeded from UniProt:P00722

lacZF602A

F602A

Decreases the stability of the loop 794-804

seeded from UniProt:P00722

lacZG795A

G795A

It forces the apoenzyme to adopt the closed rather than the open conformation. Reduces the binding affinity

seeded from UniProt:P00722

lacZE798A,L

E798A,L

The catalytic efficiency is not increased, when the sodium concentration increases

seeded from UniProt:P00722

lacZE798D,Q

E798D,Q

Small increase of the catalytic efficiency, when the sodium concentration increases

seeded from UniProt:P00722

lacZH541E,F,N

H541E,F,N

Poorly reactive with galactosyl substrates. Less stable to heat than wild-type

seeded from UniProt:P00722

lacZE538Q

E538Q

10000-fold decrease in the beta- galactosidase activity

seeded from UniProt:P00722

lacZE462H

E462H

Slowly inactivates galactosidase activity by reducing the binding of magnesium. It increases binding specificity

seeded from UniProt:P00722

lacZH358D,F,L,N

H358D,F,L,N

Less stable to heat than wild-type. Causes significant destabilizations of the first transition state

seeded from UniProt:P00722

lacZH392E,F,K

H392E,F,K

Essentially inactive unless very rapid purification. Causes very large destabilizations of the transition state

seeded from UniProt:P00722

lacZ4

CGSC:4649

lacZ43(Fs)

CGSC:4545

frameshift mutation

lacZ53(Am)

CGSC:4692

amber (UAG) mutation

lacZ36

CGSC:5084

lacZ125(Am)

PMID:5327654[4]

CGSC:5303

amber (UAG) mutation

lacZ73

CGSC:5701

lacZ46

CGSC:5741

lacZ827(UGA)

CGSC:5781

opal (UGA) mutation

lacZ82(Am)

CGSC:5996

amber (UAG) mutation

lacZ39(del)

CGSC:6051

lacZ13(Oc)

CGSC:6357

ochre (UAA) mutation

lacZ90(Oc)

PMID:6790520[5] PMID:5327654[4]

CGSC:6678

ochre (UAA) mutation

lacZ625(Am)

PMID:5327654[4]

CGSC:6682

amber (UAG) mutation

lacZ118(Oc)

PMID:5327654[4] PMID:412841[6] PMID:4876923[7]

CGSC:6685

ochre (UAA) mutation

lacZ659(Oc)

PMID:5327654[4]

CGSC:6687

ochre (UAA) mutation

lacZ404(Oc)

PMID:5327654[4]

CGSC:6690

ochre (UAA) mutation

lacZ521(UGA)

PMID:4915862[8]

CGSC:6930

opal (UGA) mutation

lacZ813(UGA)

PMID:4915862[8]

CGSC:6935

opal (UGA) mutation

lacZ105(Am)

CGSC:7341

amber (UAG) mutation

lacZ178

CGSC:7401

lacZ281(Am)

CGSC:7951

amber (UAG) mutation

lacZ57(del)

CGSC:7985

lacZ62

CGSC:8000

lacZ608(Am)

PMID:5327654[4] PMID:4915862[8]

CGSC:8396

amber (UAG) mutation

lacZ84

CGSC:8606

lacZ75(Fs)

CGSC:8609

frameshift mutation

lacZ482(Am)

CGSC:8713

amber (UAG) mutation

lacZ58(del)(M15)

CGSC:8749

lacZ98::Tn10

CGSC:8864

lacZ545(Am)

CGSC:8947

amber (UAG) mutation

lacZ2286(Am)

CGSC:9797

amber (UAG) mutation

lacZ624(Am)

PMID:4915862[8]

CGSC:10716

amber (UAG) mutation

lacZ95(del)

CGSC:10738

lacZ114((delH))

CGSC:10830

lacZ55

CGSC:11758

lacZ2210(Am)

CGSC:11841

amber (UAG) mutation

lacZ332(Fs)

PMID:1092652[9]

CGSC:12130

lacZ4503(Am)

CGSC:12975

amber (UAG) mutation

lacZ49

CGSC:16030

lacZ56

CGSC:16066

lacZ131

CGSC:19612

lacZ2246

CGSC:19613

lacZ8(Am)

PMID:2141650[10]

CGSC:23460

amber (UAG) mutation

lacZ59

CGSC:26947

lacZ4502::Tn10

PMID:337110[11]

CGSC:59813

lacZ4787(del)(::rrnB-3)

PMID:10829079[12]

CGSC:64656

lacZ4796::Tn5

CGSC:76213

lacZ536(Am)

CGSC:83183

amber (UAG) mutation

lacZ571(Am)

PMID:2501784[13]

CGSC:83642

amber (UAG) mutation

lacZ8305::Mu cts62

PMID:1111215[14]

CGSC:84395

lacZ4525::Tn10kan

PMID:9139905[15]

CGSC:85232

lacZ572

PMID:2501784[13]

CGSC:89114

lacZ574

PMID:2501784[13]

CGSC:89115

lacZ573

PMID:2501784[13]

CGSC:89116

lacZ575

PMID:2501784[13]

CGSC:89118

lacZ576

PMID:2501784[13]

CGSC:89119

lacZ1125

PMID:4915862[8] PMID:5327654[4]

CGSC:89575


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW0335

Plasmid clone

Shigen

PMID:16769691[16]

Status:Clone OK

Primer 1:GCCACCATGATTACGGATTCACT

Primer 2:CCTTTTTGACACCAGACCAACTG

10A6

Kohara Phage

Genobase

PMID:3038334[17]

proA81::Tn10

Linked marker

CAG18447 = CGSC7330[18]

est. P1 cotransduction: % [19]

lacI3042::Tn10

Linked marker

CAG18439 = CGSC7334[18]

est. P1 cotransduction: 87% [19]
Synonyms:lacI42::Tn10 nnnCAG18439 also carries lacZ118(0c) (CGSC).

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10527

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10527

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000520

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:945006

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0522

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0001183

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ Fowler, AV & Zabin, I (1978) Amino acid sequence of beta-galactosidase. VIII. Sequence of the NH2-terminal segment, CNBr peptides 1 to 9, residues 1 to 377. J. Biol. Chem. 253 5505-9 PubMed
  3. ↑ Fowler, AV & Zabin, I (1978) Amino acid sequence of beta-galactosidase. XI. Peptide ordering procedures and the complete sequence. J. Biol. Chem. 253 5521-5 PubMed
  4. ↑ 4.0 4.1 4.2 4.3 4.4 4.5 4.6 4.7 Newton, WA et al. (1965) Nonsense mutants and polarity in the lac operon of Escherichia coli. J. Mol. Biol. 14 290-6 PubMed
  5. ↑ Mandecki, W et al. (1981) Position of the lacZX90 mutation and hybridization between complete and incomplete beta-galactosidase. J. Bacteriol. 147 694-7 PubMed
  6. ↑ Zabin, I et al. (1978) Position of the mutation in beta-galactosidase ochre mutant U118. J. Bacteriol. 133 437-8 PubMed
  7. ↑ Epstein, W (1967) Transposition of the lac region of Escherichia coli. IV. Escape from repression in bacteriophage-carried lac genes. J. Mol. Biol. 30 529-43 PubMed
  8. ↑ 8.0 8.1 8.2 8.3 8.4 Zipser, D et al. (1970) Fine structure of the gradient of polarity in the z gene of the lac operon of Escherichia coli. J. Mol. Biol. 49 251-4 PubMed
  9. ↑ Hoess, RH & Herman, RK (1975) Isolation and characterization of mutator strains of Escherichia coli K-12. J. Bacteriol. 122 474-84 PubMed
  10. ↑ Normanly, J et al. (1990) Construction of Escherichia coli amber suppressor tRNA genes. III. Determination of tRNA specificity. J. Mol. Biol. 213 719-26 PubMed
  11. ↑ Foster, TJ (1977) Insertion of the tetracycline resistance translocation unit Tn10 in the lac operon of Escherichia coli K12. Mol. Gen. Genet. 154 305-9 PubMed
  12. ↑ Datsenko, KA & Wanner, BL (2000) One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc. Natl. Acad. Sci. U.S.A. 97 6640-5 PubMed
  13. ↑ 13.0 13.1 13.2 13.3 13.4 13.5 Cupples, CG & Miller, JH (1989) A set of lacZ mutations in Escherichia coli that allow rapid detection of each of the six base substitutions. Proc. Natl. Acad. Sci. U.S.A. 86 5345-9 PubMed
  14. ↑ Bukhari, AI & Allet, B (1975) Plaque-forming lambda-Mu hybrids. Virology 63 30-9 PubMed
  15. ↑ Reddy, M & Gowrishankar, J (1997) Identification and characterization of ssb and uup mutants with increased frequency of precise excision of transposon Tn10 derivatives: nucleotide sequence of uup in Escherichia coli. J. Bacteriol. 179 2892-9 PubMed
  16. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  17. ↑ Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  18. ↑ 18.0 18.1 CGSC: The Coli Genetics Stock Center
  19. ↑ 19.0 19.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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