proA:Gene
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| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Location(s) and DNA Sequence | Sequence Features | Alleles and Phenotypes | Genetic Interactions | Genetic Resources | Accessions | Links | References | Suggestions |
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Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
| Standard name |
proA |
|---|---|
| Mnemonic |
Proline |
| Synonyms |
ECK0244, b0243, JW0233, pro1, pro(1)[1], pro |
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Notes
Location(s) and DNA Sequence
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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
|
MG1655 |
5.62 minutes |
MG1655: 260727..261980 |
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NC_012967: 265995..267248 |
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NC_012759: 260726..261979 |
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W3110 |
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W3110: 260727..261980 |
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DH10B: 234831..236084 |
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Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
| Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
|---|---|---|---|---|---|
|
Coding Start (SO:0000323) |
260727 |
Edman degradation |
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Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
| Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
|---|---|---|---|---|---|---|---|
|
ΔproA (Keio:JW0233) |
deletion |
deletion |
Auxotrophies |
Requires proline for growth |
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proA::Tn5KAN-2 (FB20067) |
Insertion at nt 192 in Plus orientation |
Auxotrophies |
Requires proline for growth |
does not contain pKD46 | |||
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proA81::Tn10 |
Insertion at 261,286 bp in MG1655 (NC_000913) |
Auxotrophies |
Requires proline for growth |
adapted from Nichols et al.[6] |
Synonyms: | ||
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proA761(del)::kan |
insertion |
insertion |
Auxotrophies |
Requires proline for growth |
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proA23 |
Auxotrophies |
Requires proline for growth |
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proA44 |
Auxotrophies |
Requires proline for growth |
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proA81::Tn10 |
Auxotrophies |
Requires proline for growth |
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proA35 |
Auxotrophies |
Requires proline for growth |
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proA30 |
Auxotrophies |
Requires proline for growth |
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proA181 |
Auxotrophies |
Requires proline for growth |
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proA180 |
Auxotrophies |
Requires proline for growth |
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proA |
Auxotrophies |
Requires proline for growth |
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proA3096::Tn10kan |
Auxotrophies |
Requires proline for growth |
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ΔproA761::kan |
deletion |
deletion |
Auxotrophies |
Requires proline for growth |
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|
proA |
Resistant to |
Resistant to Nitro(4)pyridine N-oxide |
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|
proA |
Resistant to |
Nitro-4-pyridine N-oxide Resistance |
Strain: W3630 |
table 1 | |||
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Notes
Genetic Interactions
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| Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
|---|---|---|---|---|---|---|
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Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
|
JW0233 |
Plasmid clone |
Status:Clone OK Primer 1:GCCCTGGAACAAATGGGCATTGC Primer 2:CCCGCACGAATGGTGTAATCACC | |
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Kohara Phage |
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Kohara Phage |
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|
Linked marker |
est. P1 cotransduction: 33% [6] | ||
|
Linked marker |
est. P1 cotransduction: % [6] | ||
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Notes
Links
| Name | URL | Comments |
|---|---|---|
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ Deutch, AH et al. (1984) Analysis of the Escherichia coli proBA locus by DNA and protein sequencing. Nucleic Acids Res. 12 6337-55 PubMed
- ↑ 3.0 3.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
- ↑ 4.0 4.1 4.2 4.3 CGSC: The Coli Genetics Stock Center
- ↑ Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
- ↑ 6.0 6.1 6.2 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
- ↑ 7.0 7.1 Nichols, BP et al. (1998) Sequence analysis of Tn10 insertion sites in a collection of Escherichia coli strains used for genetic mapping and strain construction. J. Bacteriol. 180 6408-11 PubMed
- ↑ Singer, M et al. (1989) A collection of strains containing genetically linked alternating antibiotic resistance elements for genetic mapping of Escherichia coli. Microbiol. Rev. 53 1-24 PubMed
- ↑ Berg, CM & Rossi, JJ (1974) Proline excretion and indirect suppression in Escherichia coli and Salmonella typhimurium. J. Bacteriol. 118 928-34 PubMed
- ↑ Fayerman, JT et al. (1979) ilvU, a locus in Escherichia coli affecting the derepression of isoleucyl-tRNA synthetase and the RPC-5 chromatographic profiles of tRNAIle and tRNAVal. J. Biol. Chem. 254 9429-40 PubMed
- ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
- ↑ 12.0 12.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
