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W3110: 1294499..1294086<br /><gbrowseImage>
W3110: 1294499..1294086<br /><gbrowseImage>
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{{GBrowseLink|W3110|AC_000091|1293086|1295499|GBrowse(W3110)}}  
{{GBrowseLink|W3110|NC_007779|1293086|1295499|GBrowse(W3110)}}  
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{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="X46a9c2b2729c5"  class=" tableEdit Gene_sequence_features_table"   
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{| {{Prettytable}} id="P46a9c2b2b4118"  class=" tableEdit Gene_allele_table dataTable"   
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="P46a9c2b2b4118"  class=" tableEdit Gene_allele_table dataTable"   
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!|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments
!|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments
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&Delta;hns (Keio:JW1225)
''hns(del)'' (Keio:JW1225)
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deletion
deletion
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PMID:16738554
PMID:16738554
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[http://www.shigen.nig.ac.jp/ecoli/strain/keioCollectionQueryAction.do Shigen]<br>[http://cgsc2.biology.yale.edu/Strain.php?ID=107392 CGSC9111]<ref name='LIB:cgsc'/>  
[http://www.shigen.nig.ac.jp/ecoli/strain/keioCollectionQueryAction.do Shigen]
 
[http://cgsc2.biology.yale.edu/Strain.php?ID=107392 CGSC9111]<ref name='LIB:cgsc'/>  
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&Delta;hns::kan
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deletion
deletion
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&Delta;hns::kan
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deletion
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&Delta;hns::kan
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deletion
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&Delta;hns::kan
''hns(del)''::kan
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deletion
deletion
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&Delta;hns::kan
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deletion
deletion
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&Delta;hns::kan
''hns(del)''::kan
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deletion
deletion
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&Delta;hns::kan
''hns(del)''::kan
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deletion
deletion
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&Delta;hns::kan
''hns(del)''::kan
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deletion
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&Delta;hns::kan
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deletion
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&Delta;hns::kan
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&Delta;hns::kan
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deletion
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&Delta;hns::kan
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deletion
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''&#916;hns-746::kan''
''hns(del)-746::kan''
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fig 2  
fig 2  
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''hns-206::amp''
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Auxotrophies
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Inactivation of ''hns'' strongly reduced the SOS induction of ''sbmC'' compared to the wild type, fig 1B. same applies to double mutant ''hns'' ''rpoS''.
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PMID:11689018
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''bglY202''
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Carbon utilization
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Utilization of arbutin (a Beta-glucoside) as a carbon source, table 3.
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PMID:6266910
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Experimental Strain: FA31
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''bglY203''
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Carbon utilization
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able to utilize arbutin and salicin as a carbon source, table 3.
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PMID:6266910
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FA35, FA37, FA39, FS20, FS29
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Mutations also utilizing similar carbon sources are: ''bglY204'', ''bglY205'', ''bglY206'', ''bglY207''


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!|Interactor!!Interaction!!Allele!!Score(s)!!Reference(s)!!Accessions!!Notes
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!|Database!!Accession!!Notes
!|Database!!Accession!!Notes
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[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])
[[EcoCyc]]
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[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=EG10457 ECOCYC:EG10457]
EcoCyc:EG10457
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Escherichia coli str. K-12 substr. MG1655  
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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[[EcoGene]]
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EcoGene:EG10457
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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[http://ecogene.org EcoGene] ([[EcoGene|EcoliWiki Page]])
[[RegulonDB]]
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[http://ecogene.org/geneinfo.php?eg_id=EG10457 EcoGene:EG10457]
RegulonDB:ECK120000450
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Escherichia coli str. K-12 substr. MG1655  
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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Escherichia coli str. K-12 substr. MG1655  
Escherichia coli str. K-12 substr. MG1655  
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[[EchoBASE]]
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EchoBASE:EB0452
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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[[ASAP]]
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ASAP:ABE-0004152
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]


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Latest revision as of 20:30, 19 September 2013

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

hns

Mnemonic

Histone-like protein H-NS

Synonyms

ECK1232, b1237, JW1225, bglY, cur, drc, drdX, drs, irk, mssyA, msyA, osmZ, pilG, topX, virR, hnsA, topS, B1, H1[1], fimG, H1, verA

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

27.84 minutes 

MG1655: 1292145..1291732
<gbrowseImage> name=NC_000913:1291732..1292145 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 1292716..1292303
<gbrowseImage> name=NC_012967:1292303..1292716 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 1181230..1181643
<gbrowseImage> name=NC_012759:1181230..1181643 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 1294499..1294086
<gbrowseImage> name=NC_007779:1294086..1294499 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 1332386..1331973
<gbrowseImage> name=NC_010473:1331973..1332386 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

1291735

Edman degradation

PMID:2020545[2]
PMID:2126011[3]
PMID:6370250[4]
PMID:8740179[5]
PMID:9298646[6]
PMID:9600841[7]
PMID:9740056[8]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

hns(del) (Keio:JW1225)

deletion

deletion

PMID:16738554[9]

Shigen

CGSC9111[10]

hns::Tn5KAN-2 (FB20271)

Insertion at nt 323 in Plus orientation

PMID:15262929[11]

E. coli Genome Project:FB20271

does not contain pKD46

hns(del)::kan

deletion

Biolog:respiration

unable to respire D-Galactoniate

PMID:16095938[12]

hns(del)::kan

deletion

Biolog:respiration

unable to respire D-Galacturonate

PMID:16095938[12]

hns(del)::kan

deletion

Biolog:respiration

unable to respire D-Glucuronate

PMID:16095938[12]

hns(del)::kan

deletion

Biolog:respiration

unable to respire Acetate

PMID:16095938[12]

hns(del)::kan

deletion

Biolog:respiration

unable to respire a-Hydroxybutyrate

PMID:16095938[12]

hns(del)::kan

deletion

Biolog:respiration

unable to respire a-Ketobutyrate

PMID:16095938[12]

hns(del)::kan

deletion

Biolog:respiration

unable to respire a-Ketoglutarate

PMID:16095938[12]

hns(del)::kan

deletion

Biolog:respiration

unable to respire Succinate

PMID:16095938[12]

hns(del)::kan

deletion

Biolog:respiration

unable to respire Bromosuccinate

PMID:16095938[12]

hns(del)::kan

deletion

Biolog:respiration

unable to respire L-Alanine

PMID:16095938[12]

hns(del)::kan

deletion

Biolog:respiration

unable to respire L-Asparagine

PMID:16095938[12]

hns(del)::kan

deletion

Biolog:respiration

unable to respire L-Aspartate

PMID:16095938[12]

hns-206

CGSC:19435

hns-1

CGSC:26967

hns(del)-746::kan

PMID:16738554[9]

CGSC:101388

hns

deletion

Sensitivity to

increases sensitivity to bicyclomycin

PMID:21357484[13]

fig 2

hns-206::amp

Auxotrophies

Inactivation of hns strongly reduced the SOS induction of sbmC compared to the wild type, fig 1B. same applies to double mutant hns rpoS.

PMID:11689018[14]

bglY202

Carbon utilization

Utilization of arbutin (a Beta-glucoside) as a carbon source, table 3.

PMID:6266910[15]

Experimental Strain: FA31

bglY203

Carbon utilization

able to utilize arbutin and salicin as a carbon source, table 3.

PMID:6266910[15]

FA35, FA37, FA39, FS20, FS29

Mutations also utilizing similar carbon sources are: bglY204, bglY205, bglY206, bglY207

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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW1225

Plasmid clone

Shigen

PMID:16769691[16]

Status:Clone OK

Primer 1:GCCAGCGAAGCACTTAAAATTCT

Primer 2:CCTTGCTTGATCAGGAAATCGTC

4D8

Kohara Phage

Genobase

PMID:3038334[17]

3D5

Kohara Phage

Genobase

PMID:3038334[17]

fadR13::Tn10

Linked marker

CAG18497 = CGSC7365[10]

est. P1 cotransduction: 5% [18]

oppC506::Tn10

Linked marker

CAG12169 = CGSC7369[10]

est. P1 cotransduction: 66% [18]
Synonyms:zch-506::Tn10, zci-506::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10457

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10457

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000450

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:945829

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0452

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0004152

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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<protect>

References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ Kajitani, M & Ishihama, A (1991) Identification and sequence determination of the host factor gene for bacteriophage Q beta. Nucleic Acids Res. 19 1063-6 PubMed
  3. ↑ Yamada, H et al. (1990) An Escherichia coli protein that preferentially binds to sharply curved DNA. J. Biochem. 108 420-5 PubMed
  4. ↑ Laine, B et al. (1984) A DNA-binding protein from E. coli isolation, characterization and its relationship with proteins H1 and B1. Biochem. Biophys. Res. Commun. 119 1147-53 PubMed
  5. ↑ Pasquali, C et al. (1996) Two-dimensional gel electrophoresis of Escherichia coli homogenates: the Escherichia coli SWISS-2DPAGE database. Electrophoresis 17 547-55 PubMed
  6. ↑ Link, AJ et al. (1997) Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12. Electrophoresis 18 1259-313 PubMed
  7. ↑ Wilkins, MR et al. (1998) Protein identification with N and C-terminal sequence tags in proteome projects. J. Mol. Biol. 278 599-608 PubMed
  8. ↑ Tonella, L et al. (1998) '98 Escherichia coli SWISS-2DPAGE database update. Electrophoresis 19 1960-71 PubMed
  9. ↑ 9.0 9.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  10. ↑ 10.0 10.1 10.2 CGSC: The Coli Genetics Stock Center
  11. ↑ Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
  12. ↑ 12.00 12.01 12.02 12.03 12.04 12.05 12.06 12.07 12.08 12.09 12.10 12.11 Ito, M et al. (2005) Functional analysis of 1440 Escherichia coli genes using the combination of knock-out library and phenotype microarrays. Metab. Eng. 7 318-27 PubMed
  13. ↑ Tran, L et al. (2011) Single-gene deletion mutants of Escherichia coli with altered sensitivity to bicyclomycin, an inhibitor of transcription termination factor Rho. J. Bacteriol. 193 2229-35 PubMed
  14. ↑ Oh, TJ et al. (2001) The Escherichia coli SOS gene sbmC is regulated by H-NS and RpoS during the SOS induction and stationary growth phase. Biochem. Biophys. Res. Commun. 288 1052-8 PubMed
  15. ↑ 15.0 15.1 Defez, R & De Felice, M (1981) Cryptic operon for beta-glucoside metabolism in Escherichia coli K12: genetic evidence for a regulatory protein. Genetics 97 11-25 PubMed
  16. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  17. ↑ 17.0 17.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  18. ↑ 18.0 18.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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