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At a Glance
Recombineering (Making chromosomal deletions of genes with FRT sites)
|Origin of Replication||
|Link to Sequence|
Temperature sensitive replication (repA101ts); encodes lambda Red genes (exo, bet, gam); native terminator (tL3) after exo gene; arabinose-inducible promoter for expression (ParaB); encodes araC for repression of ParaB promoter; Ampicillin resistant.
Described by Datsenko and Wanner. Without antibiotic selection, this plasmid can be cured from a strain with growth at 37°C - 42°C. For plasmid maintenance, the strain should be grown at 30°C.
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See Help:References for how to manage references in EcoliWiki.
- Datsenko, KA & Wanner, BL (2000) One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc. Natl. Acad. Sci. U.S.A. 97 6640-5 PubMed
You can help EcoliWiki by providing database accessions or, if none are available, the sequence of this plasmid.