pKD46

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See Help:Plasmid for more information about plasmids in EcoliWiki.


At a Glance

pKD46, Image made with PlasMapper

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Type

Recombineering (Making chromosomal deletions of genes with FRT sites)

Origin of Replication

oriR101 w/repA101ts

Copy #

Low

Markers

AmpR

Link to Sequence

Sequence

Features

Temperature sensitive replication (repA101ts); encodes lambda Red genes (exo, bet, gam); native terminator (tL3) after exo gene; arabinose-inducible promoter for expression (ParaB); encodes araC for repression of ParaB promoter; Ampicillin resistant.

Accession Number

AY048746

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Notes

Described by Datsenko and Wanner[1]. Without antibiotic selection, this plasmid can be cured from a strain with growth at 37°C - 42°C. For plasmid maintenance, the strain should be grown at 30°C.

History

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Sources

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CGSC

References

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  1. ↑ Datsenko, KA & Wanner, BL (2000) One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc. Natl. Acad. Sci. U.S.A. 97 6640-5 PubMed

Sequence

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