PMID:2020545
| Citation |
Kajitani, M and Ishihama, A (1991) Identification and sequence determination of the host factor gene for bacteriophage Q beta. Nucleic Acids Res. 19:1063-6 |
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| Abstract |
The host factor (HF-I) required for phage Q beta RNA-directed synthesis of complementary minus-strand RNA was purified to homogeneity from phage-infected Escherichia coli cells. The hfq gene encoding HF-I was cloned using synthetic probes designed based on the partial amino acid sequence of HF-I, and mapped at 94.8 min on the E. coli chromosome downstream of the miaA gene involved in 2-methylthio-N6-(isopentyl)-adenosine (ms2i6A) tRNA modification. Sequence determination of the cloned hfq gene indicated that HF-I is a small protein of Mr 11,166 consisting of 102 amino acid residues. |
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| Keywords |
Amino Acid Sequence; Base Sequence; Carrier Proteins/genetics; Carrier Proteins/isolation & purification; Cloning, Molecular; Coliphages/genetics; DNA, Bacterial; DNA-Binding Proteins/genetics; DNA-Binding Proteins/isolation & purification; Escherichia coli/genetics; Escherichia coli Proteins; Genes, Bacterial; Host Factor 1 Protein; Integration Host Factors; Molecular Sequence Data; Restriction Mapping; Sequence Homology, Nucleic Acid |
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