PMID:2126011
| Citation |
Yamada, H, Muramatsu, S and Mizuno, T (1990) An Escherichia coli protein that preferentially binds to sharply curved DNA. J. Biochem. 108:420-5 |
|---|---|
| Abstract |
We attempted to find Escherichia coli proteins which preferentially bind to a curved DNA sequence even in the presence of an excess amount of a non-curved DNA sequence as a competitor, mainly by means of a DNA-binding gel retardation assay. Since the two sequences used had nearly the same nucleotide compositions, including consecutive dA5 stretches, we reasoned that this strategy would allow us to identify proteins which preferentially recognize an overall DNA curvature. We purified such a protein from E. coli. Its preferential binding to the curved DNA was found to be inhibited by distamycin, which removes the curvature from appropriate DNA sequences. The purified protein was identified as the E. coli nucleoid protein, H-NS. |
| Links | |
| Keywords |
Amino Acid Sequence; Bacterial Proteins/metabolism; Base Sequence; Binding, Competitive; DNA/metabolism; DNA-Binding Proteins/metabolism; Distamycins/pharmacology; Escherichia coli/drug effects; Escherichia coli/genetics; Molecular Sequence Data; Molecular Weight |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.
