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Ito, M, Baba, T, Mori, H and Mori, H (2005) Functional analysis of 1440 Escherichia coli genes using the combination of knock-out library and phenotype microarrays. Metab. Eng. 7:318-27


Escherichia coli is one of the best elucidated organisms. However, about 40% of E. coli genes have not been assigned to their function yet. We analyzed 1440 single gene knock-out mutants using the GN2-MicroPlate, which permits assay of 95 carbon-source utilizations simultaneously. In the knock-out library there are 1044 of so called y-genes with no apparent function. The raw dataset was analyzed and genes were interrelated by the clustering method of the GeneSpring software. In the resulted dendrogram of genes, a group of genes with known and related function tended to be assembled into a cluster. Our clustering method would be useful for functional assignment of so called y-genes with no apparent function, since the resulted dendrogram could connect y-genes to phenotype and function of well-studied genes.


PubMed Online version:10.1016/j.ymben.2005.06.004


Escherichia coli/genetics; Gene Deletion; Gene Expression Profiling; Gene Expression Regulation, Bacterial/genetics; Genes, Bacterial/genetics; Oligonucleotide Array Sequence Analysis/methods; Phenotype; Software


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