fadR:Gene
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| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Location(s) and DNA Sequence | Sequence Features | Alleles and Phenotypes | Genetic Interactions | Genetic Resources | Accessions | Links | References | Suggestions |
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Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
| Standard name |
fadR |
|---|---|
| Mnemonic |
Fatty acid degradation |
| Synonyms |
ECK1175, b1187, JW1176, dec, ole, thdB, oleR[1], oleR |
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Notes
Location(s) and DNA Sequence
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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
|
MG1655 |
26.6 minutes |
MG1655: 1234161..1234880 |
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NC_012967: 1235088..1235807 |
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NC_012759: 1122882..1123601 |
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W3110 |
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W3110: 1236515..1237234 |
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DH10B: 1274402..1275121 |
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Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
| Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
|---|---|---|---|---|---|
|
Coding Start (SO:0000323) |
1234164 |
Edman degradation |
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Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
| Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
|---|---|---|---|---|---|---|---|
|
fadR(del) (Keio:JW1176) |
deletion |
deletion |
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fadR::Tn5KAN-2 (FB20265) |
Insertion at nt 521 in Plus orientation |
does not contain pKD46 | |||||
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fadR(del)::kan |
deletion |
Biolog:respiration |
unable to respire a-Ketobutyrate |
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fadR(del)::kan |
deletion |
Biolog:respiration |
unable to respire Bromosuccinate |
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fadR(del)::kan |
deletion |
Biolog:respiration |
unable to respire L-Aspartate |
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fadR13::Tn10 |
Insertion at 1,234,734 bp in MG1655 (NC_000913) |
adapted from Nichols et al.[7] |
Synonyms: | ||||
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fadR200 |
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fadR201 |
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fadR613::Tn10 |
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fadR16 |
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fadR202 |
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fadR601 |
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fadR41(ts) |
temperature sensitive |
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fadR3115::Tn10kan |
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fadR776(del)::kan |
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fadR &thdA1 in MW5 |
Mutation in the fadR & thdA genes |
Resistant to |
Resistance to thiophene and furan derivatives |
It was found that the fadR mutation was required in conjunction with the thdA mutation for thiophene and furan derivative resistance. See table 3 for full oxidation experiment results. | |||
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Notes
Genetic Interactions
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| Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
|---|---|---|---|---|---|---|
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Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
|
JW1176 |
Plasmid clone |
Status:Clone OK Primer 1:GCCGTCATTAAGGCGCAAAGCCC Primer 2:CCTCGCCCCTGAATGGCTAAATC | |
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Kohara Phage |
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Kohara Phage |
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Linked marker |
est. P1 cotransduction: 42% [7] | ||
|
Linked marker |
est. P1 cotransduction: 2% [7] | ||
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Notes
Links
| Name | URL | Comments |
|---|---|---|
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ DiRusso, CC et al. (1992) Characterization of FadR, a global transcriptional regulator of fatty acid metabolism in Escherichia coli. Interaction with the fadB promoter is prevented by long chain fatty acyl coenzyme A. J. Biol. Chem. 267 8685-91 PubMed
- ↑ 3.0 3.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
- ↑ 4.0 4.1 4.2 4.3 CGSC: The Coli Genetics Stock Center
- ↑ Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
- ↑ 6.0 6.1 6.2 Ito, M et al. (2005) Functional analysis of 1440 Escherichia coli genes using the combination of knock-out library and phenotype microarrays. Metab. Eng. 7 318-27 PubMed
- ↑ 7.0 7.1 7.2 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
- ↑ 8.0 8.1 Singer, M et al. (1989) A collection of strains containing genetically linked alternating antibiotic resistance elements for genetic mapping of Escherichia coli. Microbiol. Rev. 53 1-24 PubMed
- ↑ Juhl, MJ & Clark, DP (1990) Thiophene-degrading Escherichia coli mutants possess sulfone oxidase activity and show altered resistance to sulfur-containing antibiotics. Appl. Environ. Microbiol. 56 3179-85 PubMed
- ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
- ↑ 11.0 11.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
