hns:On One Page

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Quickview | Gene | Product(s) | Expression| Evolution | References |

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Standard Name

hns

Gene Synonym(s)

ECK1232, b1237, JW1225, bglY, cur, drc, drdX, drs, irk, mssyA, msyA, osmZ, pilG, topX, virR, hnsA, topS, B1, H1[1], fimG, H1, verA

Product Desc.

H-NS transcriptional dual regulator[2][3]

DNA-binding global regulator H-NS; diverse mutant phenotypes affecting transcription, transposition, inversion, cryptic-gene expression; involved in chromosome organization; preferentially binds bent DNA; has N-terminal dimerization domain[4]

Product Synonyms(s)

global DNA-binding transcriptional dual regulator H-NS[1], B1237[2][1], Irk[2][1], MsyA[2][1], PilG[2][1], TopS[2][1], TopX[2][1], VirR[2][1], Drs[2][1], Drc[2][1], Cur[2][1], Hns[2][1], DrdX[2][1], BglY[2][1], HnsA[2][1], DNA-binding protein HLP-II (HU, BH2, HD, NS); pleiotropic regulator[2][1], OsmZ[2][1], DNA-binding protein HLP-II (HU, BH2, HD, NS)[3][1] , B1, bglY, cur, drc, drdX, drs, ECK1232, fimG, H1, hnsA, irk, JW1225, msyA, osmZ, pilG, topS, topX, verA, virR, b1237

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): hns[2], OP00190

Regulation/Activity
Quick Links

porteco.png EcoCyc.gif regulondb.jpg

dnadisplay.png proteindisplay.png   pubmed.jpg   textpresso.jpg  

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Notes

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HN-S forms heteromeric complexes with Hha and Cnu, which modulates HN-S function, expression and stability. Mutations affect chromosomal supercoiling. HT_Cmplx32_Mem: CspC+Hns+HupA.[4]



Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

hns

Mnemonic

Histone-like protein H-NS

Synonyms

ECK1232, b1237, JW1225, bglY, cur, drc, drdX, drs, irk, mssyA, msyA, osmZ, pilG, topX, virR, hnsA, topS, B1, H1[1], fimG, H1, verA

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

27.84 minutes 

MG1655: 1292145..1291732
<gbrowseImage> name=NC_000913:1291732..1292145 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 1292716..1292303
<gbrowseImage> name=NC_012967:1292303..1292716 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 1181230..1181643
<gbrowseImage> name=NC_012759:1181230..1181643 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 1294499..1294086
<gbrowseImage> name=NC_007779:1294086..1294499 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 1332386..1331973
<gbrowseImage> name=NC_010473:1331973..1332386 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

1291735

Edman degradation

PMID:2020545
PMID:2126011
PMID:6370250
PMID:8740179
PMID:9298646
PMID:9600841
PMID:9740056


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

hns(del) (Keio:JW1225)

deletion

deletion

PMID:16738554

Shigen

CGSC9111[5]

hns::Tn5KAN-2 (FB20271)

Insertion at nt 323 in Plus orientation

PMID:15262929

E. coli Genome Project:FB20271

does not contain pKD46

hns(del)::kan

deletion

Biolog:respiration

unable to respire D-Galactoniate

PMID:16095938

hns(del)::kan

deletion

Biolog:respiration

unable to respire D-Galacturonate

PMID:16095938

hns(del)::kan

deletion

Biolog:respiration

unable to respire D-Glucuronate

PMID:16095938

hns(del)::kan

deletion

Biolog:respiration

unable to respire Acetate

PMID:16095938

hns(del)::kan

deletion

Biolog:respiration

unable to respire a-Hydroxybutyrate

PMID:16095938

hns(del)::kan

deletion

Biolog:respiration

unable to respire a-Ketobutyrate

PMID:16095938

hns(del)::kan

deletion

Biolog:respiration

unable to respire a-Ketoglutarate

PMID:16095938

hns(del)::kan

deletion

Biolog:respiration

unable to respire Succinate

PMID:16095938

hns(del)::kan

deletion

Biolog:respiration

unable to respire Bromosuccinate

PMID:16095938

hns(del)::kan

deletion

Biolog:respiration

unable to respire L-Alanine

PMID:16095938

hns(del)::kan

deletion

Biolog:respiration

unable to respire L-Asparagine

PMID:16095938

hns(del)::kan

deletion

Biolog:respiration

unable to respire L-Aspartate

PMID:16095938

hns-206

CGSC:19435

hns-1

CGSC:26967

hns(del)-746::kan

PMID:16738554

CGSC:101388

hns

deletion

Sensitivity to

increases sensitivity to bicyclomycin

PMID:21357484

fig 2

hns-206::amp

Auxotrophies

Inactivation of hns strongly reduced the SOS induction of sbmC compared to the wild type, fig 1B. same applies to double mutant hns rpoS.

PMID:11689018

bglY202

Carbon utilization

Utilization of arbutin (a Beta-glucoside) as a carbon source, table 3.

PMID:6266910

Experimental Strain: FA31

bglY203

Carbon utilization

able to utilize arbutin and salicin as a carbon source, table 3.

PMID:6266910

FA35, FA37, FA39, FS20, FS29

Mutations also utilizing similar carbon sources are: bglY204, bglY205, bglY206, bglY207

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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW1225

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCAGCGAAGCACTTAAAATTCT

Primer 2:CCTTGCTTGATCAGGAAATCGTC

4D8

Kohara Phage

Genobase

PMID:3038334

3D5

Kohara Phage

Genobase

PMID:3038334

fadR13::Tn10

Linked marker

CAG18497 = CGSC7365[5]

est. P1 cotransduction: 5% [6]

oppC506::Tn10

Linked marker

CAG12169 = CGSC7369[5]

est. P1 cotransduction: 66% [6]
Synonyms:zch-506::Tn10, zci-506::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10457

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10457

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000450

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:945829

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0452

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0004152

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

Hns

Synonyms

global DNA-binding transcriptional dual regulator H-NS[1], B1237[2][1], Irk[2][1], MsyA[2][1], PilG[2][1], TopS[2][1], TopX[2][1], VirR[2][1], Drs[2][1], Drc[2][1], Cur[2][1], Hns[2][1], DrdX[2][1], BglY[2][1], HnsA[2][1], DNA-binding protein HLP-II (HU, BH2, HD, NS); pleiotropic regulator[2][1], OsmZ[2][1], DNA-binding protein HLP-II (HU, BH2, HD, NS)[3][1] , B1, bglY, cur, drc, drdX, drs, ECK1232, fimG, H1, hnsA, irk, JW1225, msyA, osmZ, pilG, topS, topX, verA, virR, b1237

Product description

H-NS transcriptional dual regulator[2][3]

DNA-binding global regulator H-NS; diverse mutant phenotypes affecting transcription, transposition, inversion, cryptic-gene expression; involved in chromosome organization; preferentially binds bent DNA; has N-terminal dimerization domain[4]

EC number (for enzymes)


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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status

GO:0003677

DNA binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001801

F

Seeded from EcoCyc (v14.0)

complete

GO:0003677

DNA binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0238

F

Seeded from EcoCyc (v14.0)

complete

GO:0005622

intracellular

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001801

C

Seeded from EcoCyc (v14.0)

complete

GO:0006350

transcription

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0804

P

Seeded from EcoCyc (v14.0)

complete

GO:0006355

regulation of transcription, DNA-dependent

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001801

P

Seeded from EcoCyc (v14.0)

complete

GO:0016020

membrane

PMID:16858726

IDA: Inferred from Direct Assay

C

Seeded from EcoCyc (v14.0)

complete

GO:0003681

bent DNA binding

PMID:10551881

IDA: Inferred from Direct Assay

F

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

ssb

PMID:15690043

Experiment(s):EBI-890481

Protein

ymfG

PMID:16606699

Experiment(s):EBI-1139271

Protein

stpA

PMID:16606699

Experiment(s):EBI-1139271

Protein

sxy

PMID:16606699

Experiment(s):EBI-1139271

Protein

prs

PMID:16606699

Experiment(s):EBI-1139271

Protein

rplN

PMID:16606699

Experiment(s):EBI-1139271

Protein

ruvC

PMID:16606699

Experiment(s):EBI-1139271

Protein

aceE

PMID:15690043

Experiment(s):EBI-890481

Protein

ynbC

PMID:15690043

Experiment(s):EBI-890481

Protein

malP

PMID:15690043

Experiment(s):EBI-890481

Protein

malQ

PMID:15690043

Experiment(s):EBI-890481

Protein

rpoB

PMID:15690043

Experiment(s):EBI-890481

Protein

rpoC

PMID:15690043

Experiment(s):EBI-890481

Protein

rpsB

PMID:15690043

Experiment(s):EBI-890481, EBI-895331

Protein

secA

PMID:15690043

Experiment(s):EBI-890481

Protein

tig

PMID:15690043

Experiment(s):EBI-890481

Protein

cnu

PMID:15690043

Experiment(s):EBI-895331

Protein

hupA

PMID:15690043

Experiment(s):EBI-895331

Protein

rplV

PMID:15690043

Experiment(s):EBI-895331

Protein

rplX

PMID:15690043

Experiment(s):EBI-895331

Protein

stpA

PMID:15690043

Experiment(s):EBI-895331

Protein

tig

PMID:19402753

LCMS(ID Probability):97.9 MALDI(Z-score):36.434009

Protein

ssb

PMID:19402753

MALDI(Z-score):29.456422

Protein

rplX

PMID:19402753

LCMS(ID Probability):99.6

Protein

hupA

PMID:19402753

LCMS(ID Probability):99.6

Protein

stpA

PMID:19402753

LCMS(ID Probability):99.6

Protein

cnu

PMID:19402753

LCMS(ID Probability):99.6

Protein

HF-1

H-NS acts upstream or at the same level that HF-1 does; H-NS influences the expression or activity of HF-1 leading to affects in rpoStranslation

PMID:8654929

there is no change in rpoS translation when there is an hns mutation when in and hfq mutant

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes

cytoplasm

From EcoCyc[3]


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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MSEALKILNN IRTLRAQARE CTLETLEEML EKLEVVVNER REEESAAAAE VEERTRKLQQ
YREMLIADGI DPNELLNSLA AVKSGTKAKR AQRPAKYSYV DENGETKTWT GQGRTPAVIK
KAMDEQGKSL DDFLIKQ
Length

137

Mol. Wt

15.539 kDa

pI

5.3 (calculated)

Extinction coefficient

9,970 - 10,095 (calc based on 3 Y, 1 W, and 1 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Initiator Methionine

1

Removed

UniProt:P0ACF8

Domain

24..130

PF00816 H-NS histone family

PMID:19920124

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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=hns taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16129198

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:945829

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0004152

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P0ACF8

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10457

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10457

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:945829

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000450

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0452

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

Protein

E. coli K-12 MC4100

2.32E+05

molecules/cell

  • Medium: median data from rich and minimal media
  • Temperature (°C): 37
  • Doubling time (min): Growth Rate(min):
  • Growth phase: other:

emPAI

PMID:18304323

Protein

E. coli K-12 EMG2

820

molecules/cell

  • Medium: MOPS glucose minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • Growth phase: Exponential
  • OD: OD600 = 0.2 to 0.25
  • other: aerated

Quantitative protein sequencing

Spot ID: Ml39

PMID: 9298646

Protein

E. coli K-12 MG1655

86090

molecules/cell/generation

  • Medium: MOPS Complete
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

28848

molecules/cell/generation

  • Medium: MOPS Minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

38867

molecules/cell/generation

  • Medium: MOPS Complete without Methionine
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

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Notes

Transcription and Transcriptional Regulation

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<protect>

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

hns

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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:1292125..1292165 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GEO Profiles:b1237 (EcoliWiki Page)

NCBI GEO profiles for hns

microarray

GenExpDB:b1237 (EcoliWiki Page)

Summary of data for hns from multiple microarray studies

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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes
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Notes

Accessions Related to hns Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10457

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0452

Escherichia coli str. K-12 substr. MG1655

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b1237

EcoGene

EcoGene:EG10457

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000450

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0004152

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism Homologs (Statistics) Comments

Shigella flexneri

HNS

From SHIGELLACYC

E. coli O157

HNS

From ECOO157CYC

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Do-It-Yourself Web Tools

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Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

Pfam (EcoliWiki Page)

PF00816 H-NS histone family

Superfamily (EcoliWiki Page)

SUPERFAMILY:81273

Superfamily (EcoliWiki Page)

SUPERFAMILY:81273

EcoCyc

EcoCyc:EG10457

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10457

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000450

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0452

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0004152

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 1.15 1.16 1.17 1.18 1.19 1.20 1.21 1.22 1.23 1.24 1.25 1.26 1.27 1.28 1.29 1.30 1.31 1.32 1.33 1.34 1.35 1.36 1.37 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 2.13 2.14 2.15 2.16 2.17 2.18 2.19 2.20 2.21 2.22 2.23 2.24 2.25 2.26 2.27 2.28 2.29 2.30 2.31 2.32 2.33 2.34 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 3.2 3.3 3.4 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ 4.0 4.1 4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
  6. ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

Categories

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