crp:Gene: Difference between revisions

From EcoliWiki
Jump to navigation Jump to search
← Older edit
Insert SeqFeature_Table link.
Table edited by Kayla.c.lesage via TableEdit
 
(25 intermediate revisions by 5 users not shown)
Line 16: Line 16:
|{{Sub}}[[#Sequence Features|Sequence Features]]
|{{Sub}}[[#Sequence Features|Sequence Features]]
|{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]]
|{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]]
|{{Sub}}[[#Genetic Interactions | Genetic Interactions]]
|{{Sub}}[[#Genetic Resources  |Genetic Resources]]
|{{Sub}}[[#Genetic Resources  |Genetic Resources]]
|{{Sub}}[[#Accessions in Other Databases |Accessions]]
|{{Sub}}[[#Accessions in Other Databases |Accessions]]
Line 32: Line 33:
*  
*  
****************************************************************************************** -->{{Gene_nomenclature_help}}
****************************************************************************************** -->{{Gene_nomenclature_help}}
{| id="K46a9f3ef40e2d"  class=" tableEdit Gene_nomenclature_table"   
{|   id="K46a9f3ef40e2d"  class=" tableEdit Gene_nomenclature_table"   


|-
|-
Line 66: Line 67:
*  
*  
****************************************************************************************** -->{{Gene_location_help}}
****************************************************************************************** -->{{Gene_location_help}}
{|   id="W46a9f3efc1dbd"  class=" tableEdit Gene_location_table"   
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="W46a9f3efc1dbd"  class=" tableEdit Gene_location_table"   
|- {{table_heading_style}}
|- {{table_heading_style}}
!|Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links
!|Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links
Line 75: Line 76:
75.09 minutes, 75.09 minutes 
75.09 minutes, 75.09 minutes 
|
|
MG1655: 3484142..3484774<br />{{GBrowseFigureByCoord|3484142|3484774|ecoli|MG1655}}
MG1655: 3484142..3484774<br /><gbrowseImage>
name=NC_000913:3484142..3484774
source=MG1655
preset=GeneLocation
</gbrowseImage>
 
|
|
*[http://biocyc.org/ECOLI/NEW-IMAGE?type=LOCUS-POSITION&chromosome=COLI-K12&object=EG10164 EcoCyc]
*[http://biocyc.org/ECOLI/NEW-IMAGE?type=LOCUS-POSITION&chromosome=COLI-K12&object=EG10164 EcoCyc]
{{GBrowseLink|ecoli|NC_000913|3483142|3485774|GBrowse(MG1655)}}
{{GBrowseLink|MG1655|NC_000913|3483142|3485774|GBrowse(MG1655)}}
   
   
|
|
{{GBrowseFastaLink|ecoli|NC_000913|3484142|3484774|FASTA Dump}}
{{GBrowseFastaLink|MG1655|NC_000913|3484142|3484774|FASTA Dump}}
*[http://biocyc.org/ECOLI/sequence?object=EG10164 EcoCyc Sequence Dump]  
*[http://biocyc.org/ECOLI/sequence?object=EG10164 EcoCyc Sequence Dump]  
|-  
|-  
Line 89: Line 95:


|
|
NC_012967: 3414025..3414657<br />{{GBrowseFigureByCoord|3414025|3414657|REL606|NC_012967}}
NC_012967: 3414025..3414657<br /><gbrowseImage>
name=NC_012967:3414025..3414657
source=REL606
preset=GeneLocation
</gbrowseImage>
 
|
|
{{GBrowseLink|REL606|NC_012967|3414025|3414657|GBrowse (REL606)}}
{{GBrowseLink|REL606|NC_012967|3414025|3414657|GBrowse (REL606)}}
Line 100: Line 111:


|
|
NC_012759: 3371299..3371931<br />{{GBrowseFigureByCoord|3371299|3371931|BW2952|NC_012759}}
NC_012759: 3371299..3371931<br /><gbrowseImage>
name=NC_012759:3371299..3371931
source=BW2952
preset=GeneLocation
</gbrowseImage>
 
|
|
{{GBrowseLink|BW2952|012759|3371299|NC_3371931|GBrowse (BW2952)}}
{{GBrowseLink|BW2952|NC_012759|3371299|3371931|GBrowse (BW2952)}}
|
|
{{GBrowseFastaLink|BW2952|012759|3371299|NC_3371931|FASTA Dump}}  
{{GBrowseFastaLink|BW2952|NC_012759|3371299|3371931|FASTA Dump}}  
|-  
|-  
|
|
Line 111: Line 127:
&nbsp;
&nbsp;
|
|
W3110: 4154296..4153664<br />{{GBrowseFigureByCoord|4153664|4154296|ecoli_W3110|W3110}}
W3110: 4154296..4153664<br /><gbrowseImage>
name=NC_007779:4153664..4154296
source=W3110
preset=GeneLocation
</gbrowseImage>
 
|
|
{{GBrowseLink|ecoli_W3110|AC_000091|4152664|4155296|GBrowse(W3110)}}  
{{GBrowseLink|W3110|NC_007779|4152664|4155296|GBrowse(W3110)}}  
|
|
{{GBrowseFastaLink|ecoli_W3110|AC_000091|4153664|4154296|FASTA Dump}}  
{{GBrowseFastaLink|W3110|NC_007779|4153664|4154296|FASTA Dump}}  
|-  
|-  
|
|
Line 122: Line 143:


|
|
DH10B: 3581887..3582519<br />{{GBrowseFigureByCoord|3581887|3582519|ecoli_DH10B|DH10B}}
DH10B: 3581887..3582519<br /><gbrowseImage>
name=NC_010473:3581887..3582519
source=DH10B
preset=GeneLocation
</gbrowseImage>
 
|
|
{{GBrowseLink|ecoli_DH10B|NC_010473|3580623|3583783|GBrowse(DH10B)}}
{{GBrowseLink|DH10B|NC_010473|3580623|3583783|GBrowse(DH10B)}}
|
|
{{GBrowseFastaLink|ecoli_DH10B|NC_010473|3580623|3583783|FASTA Dump}}  
{{GBrowseFastaLink|DH10B|NC_010473|3580623|3583783|FASTA Dump}}  


|- class="tableEdit_footer"  
|- class="tableEdit_footer"  
Line 146: Line 172:
*  
*  
****************************************************************************************** -->{{Gene_sequence_features_help}}
****************************************************************************************** -->{{Gene_sequence_features_help}}
{|   id="V46a9f3f082c0e"  class=" tableEdit Gene_sequence_features_table"   
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="V46a9f3f082c0e"  class=" tableEdit Gene_sequence_features_table"   
|- {{table_heading_style}}
|- {{table_heading_style}}
!|Feature Type!!Strain!!Genomic Location!!Evidence!!Reference!!Notes
!|Feature Type!!Strain!!Genomic Location!!Evidence!!Reference!!Notes
|-
|
Coding Start (SO:0000323)
|
[[MG1655]]
|
3484145
|
Edman degradation
|
PMID:6271171<br />
|


|- class="tableEdit_footer"  
|- class="tableEdit_footer"  
Line 168: Line 207:
*  
*  
****************************************************************************************** -->{{Gene_alleles_help}}
****************************************************************************************** -->{{Gene_alleles_help}}
{| {{Prettytable}} id="T46a9f3f0cbc49"  class=" tableEdit Gene_allele_table dataTable"   
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="T46a9f3f0cbc49"  class=" tableEdit Gene_allele_table dataTable"   
|- {{table_heading_style}}
|- {{table_heading_style}}
!|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments
!|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments
Line 326: Line 365:
|-  
|-  
|
|
&Delta;crp (Keio:JW5702)
''crp(del)'' (Keio:JW5702)
|
|
deletion
deletion
Line 343: Line 382:
|-  
|-  
|
|
[http://asap.ahabs.wisc.edu/asap/feature_info.php?FeatureID=ABE-0010970 crp::Tn5KAN]
[http://asap.ahabs.wisc.edu/asap/feature_info.php?FeatureID=ABE-0010970 crp::Tn5KAN-I-SceI] (FB21127)
|
|
 
Insertion at nt 369 in Minus orientation
|
|


Line 353: Line 392:


|
|
PMID:12519969
PMID:15262929
|
|
[[asapstrains:ASAP|ASAP]]
[http://www.genome.wisc.edu/resources/forms/strainrequest.htm E. coli Genome Project:FB21127]
|
|
At position 369 in Minus orientation, contains plasmid  [[pKD46]].<br />ASAP strain name: FB21127
contains [[pKD46]]  
|-  
|-  
|
|
[http://asap.ahabs.wisc.edu/asap/feature_info.php?FeatureID=ABE-0010970 crp::Tn5KAN]
[http://asap.ahabs.wisc.edu/asap/feature_info.php?FeatureID=ABE-0010970 crp::Tn5KAN-I-SceI] (FB21128)
|
|
 
Insertion at nt 369 in Minus orientation
|
|


Line 370: Line 409:


|
|
PMID:12519969
PMID:15262929
|
|
[[asapstrains:ASAP|ASAP]]
[http://www.genome.wisc.edu/resources/forms/strainrequest.htm E. coli Genome Project:FB21128]
|
|
At position 369 in Minus orientation, does not contain plasmid  [[pKD46]].<br />ASAP strain name: FB21128
does not contain [[pKD46]]  
|-  
|-  
|
|
&Delta;crp::kan
crp(del)::kan
|
|
deletion
deletion
Line 394: Line 433:
|-  
|-  
|
|
&Delta;crp::kan
crp(del)::kan
|
|
deletion
deletion
Line 411: Line 450:
|-  
|-  
|
|
&Delta;crp::kan
''crp(del)''::kan
|
|
deletion
deletion
Line 428: Line 467:
|-  
|-  
|
|
&Delta;crp::kan
''crp(del)''::kan
|
|
deletion
deletion
Line 445: Line 484:
|-  
|-  
|
|
&Delta;crp::kan
''crp(del)''::kan
|
|
deletion
deletion
Line 462: Line 501:
|-  
|-  
|
|
&Delta;crp::kan
''crp(del)''::kan
|
|
deletion
deletion
Line 479: Line 518:
|-  
|-  
|
|
&Delta;crp::kan
''crp(del)''::kan
|
|
deletion
deletion
Line 496: Line 535:
|-  
|-  
|
|
&Delta;crp::kan
''crp(del)''::kan
|
|
deletion
deletion
Line 513: Line 552:
|-  
|-  
|
|
&Delta;crp::kan
''crp(del)''::kan
|
|
deletion
deletion
Line 530: Line 569:
|-  
|-  
|
|
&Delta;crp::kan
''crp(del)''::kan
|
|
deletion
deletion
Line 547: Line 586:
|-  
|-  
|
|
&Delta;crp::kan
''crp(del)''::kan
|
|
deletion
deletion
Line 564: Line 603:
|-  
|-  
|
|
&Delta;crp::kan
''crp(del)''::kan
|
|
deletion
deletion
Line 581: Line 620:
|-  
|-  
|
|
&Delta;crp::kan
''crp(del)''::kan
|
|
deletion
deletion
Line 598: Line 637:
|-  
|-  
|
|
&Delta;crp::kan
''crp(del)''::kan
|
|
deletion
deletion
Line 615: Line 654:
|-  
|-  
|
|
&Delta;crp::kan
''crp(del)''::kan
|
|
deletion
deletion
Line 632: Line 671:
|-  
|-  
|
|
&Delta;crp::kan
''crp(del)''::kan
|
|
deletion
deletion
Line 649: Line 688:
|-  
|-  
|
|
&Delta;crp::kan
''crp(del)''::kan
|
|
deletion
deletion
Line 666: Line 705:
|-  
|-  
|
|
&Delta;crp::kan
''crp(del)''::kan
|
|
deletion
deletion
Line 683: Line 722:
|-  
|-  
|
|
&Delta;crp::kan
''crp(del)''::kan
|
|
deletion
deletion
Line 700: Line 739:
|-  
|-  
|
|
&Delta;crp::kan
''crp(del)''::kan
|
|
deletion
deletion
Line 717: Line 756:
|-  
|-  
|
|
&Delta;crp::kan
''crp(del)''::kan
|
|
deletion
deletion
Line 734: Line 773:
|-  
|-  
|
|
&Delta;crp::kan
''crp(del)''::kan
|
|
deletion
deletion
Line 751: Line 790:
|-  
|-  
|
|
&Delta;crp::kan
''crp(del)''::kan
|
|
deletion
deletion
Line 768: Line 807:
|-  
|-  
|
|
&Delta;crp::kan
''crp(del)''::kan
|
|
deletion
deletion
Line 785: Line 824:
|-  
|-  
|
|
&Delta;crp::kan
''crp(del)''::kan
|
|
deletion
deletion
Line 802: Line 841:
|-  
|-  
|
|
&Delta;crp::kan
''crp(del)''::kan
|
|
deletion
deletion
Line 819: Line 858:
|-  
|-  
|
|
''&#916;crp-3''
''crp-3(del)''
|
|


Line 836: Line 875:
|-  
|-  
|
|
''&#916;crp-45''
''crp-45(del)''
|
|


Line 904: Line 943:
|-  
|-  
|
|
''&#916;crp-39''
''crp-39(del)''
|
|


Line 936: Line 975:
|
|
   
   
|-
|
''crp(del)''
|
deletion
|
deletion
|
Auxotrophies
|
Mutant unable to grow on MOPS minimal medium containing L-idonate, table 5.
|
PMID:14973046
|
experimental strain: CB370
|
|-
|
''crp(del)''
|
deletion
|
deletion
|
Auxotrophies
|
Mutant unable to grow on MOPS minimal medium containing L-idonate, table 5.
|
PMID:14973046
|
experimental strain: CB370
|
|-
|
''crp(del)''
|
deletion
|
deletion
|
Auxotrophies
|
Mutant unable to grow on MOPS minimal medium containing L-idonate, table 5.
|
PMID:14973046
|
experimental strain: CB370
|
|-
|
''cya (del) crp (del)''
|
|
|
Sensitivity to
|
sensitivity to streptozotocin
|
PMID:6450313
|
Strain: CA8445
|
table 2 and figure 3 
|-
|
''crp<sup>  -</sup>'' ''relA<sup>  +</sup>''
|
|
|
Resistant to
|
Deficient mutants in ''relA''  backgroumd, were found to be resistant to smg (serine+methionine+glycine)
|
PMID:230407
|
|
table 4
|-
|
''cya(del)'' ''crp<nowiki>*</nowiki>'' ''relA<sup>  +</sup>''
|
|
|
Resistant to
|
''crp'' allele that modified cyclic AMP receptor proteins, which acts independently of cyclic AMP, is resistant to smg (serine+methionine+glycine) in a ''relA'' background.
|
PMID:230407
|
Experimental strain: BM6163
|
table 4
|-
|
''crp'' in mutant strain B1558
|
|
|
Resistant to
|
Resistance to Beta Lactams
|
PMID:6344786
|
Strain: B1558
|
Table 2
|-
|
''crp <sup>  -</sup>''
|
|
|
Resistant to
|
resistance to Mecillinam
|
PMID:217866
|
Strain: A060
|
Table 2B
|-
|
''crp'' mutant in strain GC2846
|
|
|
Colony Morphology
|
Mutatins causes spherical morphology. Wild Type is rod shaped.
|
PMID:2826407
|
Strain: GC2846
|
See figure 1
|-
|
''Salmonella typhimurium'' LT2 ''crp''-deficient
|
|
|
Resistant to
|
Partially resistant to 22 antibiotics (including fosfomycin, nalidixic acid, and streptomycin)
|
PMID:201606
|
|
|-
|
''crp'' in strain NCR-C51
|
|
|
Resistant to
|
Resistance to Phage T6
|
|
PMID:227838
|
Table 3
|-
|
crp-403
|
|
|
Resistant to
|
Resistant to Fosfomycin
|
PMID:201606
|
Strain: TA3302
|
Parent Strain: LT2
Donor Strain: TA2305
|-
|
crp-401
|
Spontaneous nonsense mutation
|
|
Resistant to
|
Resistant to Fosfomycin
|
PMID:201606
|
Strain: TA3339
|
Parent Strain: LT2
|-
|
crp-401
|
Spontaneous nonsense mutation
|
|
Resistant to
|
Resistant to Streptomycin
|
PMID:201606
|
Strain:TA3339
|
Parent strain: LT2


|- class="tableEdit_footer"  
|- class="tableEdit_footer"  
Line 945: Line 1,223:
===Notes===
===Notes===


==Genetic Interactions==
{{BacktoTop}}
<protect><!--box uid=bc145ea147f7e36caf09c1bfeed4ee9d.17265.M4e04b9093f3fe-->
<!--
******************************************************************************************
*
*  ** PLEASE DON'T EDIT THIS TABLE DIRECTLY.  Use the edit table link under the table. **
*
****************************************************************************************** -->{{Genetic_interaction_help}}
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="M4e04b9093f3fe"  class=" tableEdit Genetic_interaction_table dataTable" 
|- {{table_heading_style}}
!|Interactor!!Interaction!!Allele!!Score(s)!!Reference(s)!!Accessions!!Notes
|- class="tableEdit_footer"
|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=bc145ea147f7e36caf09c1bfeed4ee9d.17265.M4e04b9093f3fe&page=17265&pagename={{FULLPAGENAMEE}}&type=0&template=Genetic_interaction_table edit table]</span> || || || || || ||
|}
<!--box uid=bc145ea147f7e36caf09c1bfeed4ee9d.17265.M4e04b9093f3fe--></protect>
<!-- Genetic Interacdtions Notes -->
===Notes===


==Genetic Resources==
==Genetic Resources==
Line 955: Line 1,255:
*  
*  
****************************************************************************************** -->{{Gene_resources_help}}
****************************************************************************************** -->{{Gene_resources_help}}
{|   id="B46a9f3f18cd16"  class=" tableEdit Gene_resource_table"   
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="B46a9f3f18cd16"  class=" tableEdit Gene_resource_table"   
|- {{table_heading_style}}
|- {{table_heading_style}}
!|Resource!!Resource Type!!Source!!Notes/Reference
!|Resource!!Resource Type!!Source!!Notes/Reference
Line 973: Line 1,273:


Primer 2:CCACGAGTGCCGTAAACGACGAT  
Primer 2:CCACGAGTGCCGTAAACGACGAT  
|-
|
[[:Category:Kohara_Phage:4C5|4C5]]
|
Kohara Phage
|
[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=625 Genobase]
|
PMID:3038334
|-  
|-  
|
|
Line 1,012: Line 1,321:
*  
*  
****************************************************************************************** -->{{Gene_accessions_help}}
****************************************************************************************** -->{{Gene_accessions_help}}
{|   id="K46a9f3f235238"  class=" tableEdit Gene_accessions_table"   
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="K46a9f3f235238"  class=" tableEdit Gene_accessions_table"   
|- {{table_heading_style}}
|- {{table_heading_style}}
!|Database!!Accession!!Notes
!|Database!!Accession!!Notes
|-  
|-  
|
|
[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])
[[EcoCyc]]
|
[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=EG10164 EcoCyc:EG10164]
|
|-
|
[http://www.ecoli-york.org EchoBASE] ([[EchoBASE|EcoliWiki Page]])
|
|
[http://www.york.ac.uk/res/thomas/Gene.cfm?recordID=EB0162 EchoBASE:EB0162]
EcoCyc:EG10164
|
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-  
|-  
|
|
[http://chase.ou.edu/oubcf/index.php EcoliGenExpDB] ([[EcoliGenExpDB|EcoliWiki Page]])
[[EcoGene]]
|
|
[http://chase.ou.edu/macro/searchlink.php?bnum=b3357 EcoliGenExpDB:b3357]
EcoGene:EG10164
|
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-  
|-  
|
|
[http://BioCyc.org/ECOL199310/ ECOL199310CYC] ([[ECOL199310CYC|EcoliWiki Page]])
[[RegulonDB]]
|
|
[http://BioCyc.org/ECOL199310/new-image?object=C4132 ECOL199310CYC:C4132]
RegulonDB:ECK120000160
|
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-  
|-  
|
|
[http://ecogene.org EcoGene] ([[EcoGene|EcoliWiki Page]])  
[http://www.ncbi.nlm.nih.gov/gene NCBI] ([[NCBI|EcoliWiki Page]])
|
|
[http://ecogene.org/geneinfo.php?eg_id=EG10164 EcoGene:EG10164]
[http://www.ncbi.nlm.nih.gov/gene?term=947867 GeneID:947867]
|
|
Escherichia coli str. K-12 substr. MG1655
|-  
|-  
|
|
[http://BioCyc.org/ECOO157/ ECOO157CYC] ([[ECOO157CYC|EcoliWiki Page]])
[[EchoBASE]]
|
|
[http://BioCyc.org/ECOO157/new-image?object=CRP ECOO157CYC:CRP]
EchoBASE:EB0162
|
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-  
|-  
|
|
[http://cgsc.biology.yale.edu CGSC] ([[CGSC|EcoliWiki Page]])
[[ASAP]]
|
|
[http://cgsc.biology.yale.edu/cgi-bin/sybgw/cgsc/Site/906 CGSC:906]
ASAP:ABE-0010970
|
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]  
|-  
|
[https://asap.ahabs.wisc.edu/asap/logon.php ASAP] ([[ASAP|EcoliWikiPage]])
|
[http://asap.ahabs.wisc.edu/asap/feature_info.php?LocationID=WIS&FeatureID=0010970&formSubmitReturn=1 ASAP:ABE-0010970]
|


|- class="tableEdit_footer"  
|- class="tableEdit_footer"  
Line 1,090: Line 1,385:
*  
*  
****************************************************************************************** -->
****************************************************************************************** -->
{|   id="M46a70f0d4bc28"  class=" tableEdit Links_table"   
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="M46a70f0d4bc28"  class=" tableEdit Links_table"   
|- {{table_heading_style}}
|- {{table_heading_style}}
!|Name!!URL!!Comments
!|Name!!URL!!Comments

Latest revision as of 22:11, 21 October 2013

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

Nomenclature

[back to top]


See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

crp

Mnemonic

cAMP receptor protein

Synonyms

ECK3345, b3357, JW5702, cap, csm, gurB, G51[1][2]

edit table


Notes

Location(s) and DNA Sequence

[back to top]


See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

75.09 minutes, 75.09 minutes 

MG1655: 3484142..3484774
<gbrowseImage> name=NC_000913:3484142..3484774 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 3414025..3414657
<gbrowseImage> name=NC_012967:3414025..3414657 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 3371299..3371931
<gbrowseImage> name=NC_012759:3371299..3371931 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 4154296..4153664
<gbrowseImage> name=NC_007779:4153664..4154296 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 3581887..3582519
<gbrowseImage> name=NC_010473:3581887..3582519 source=DH10B preset=GeneLocation </gbrowseImage>

edit table


Notes

Sequence Features

[back to top]


See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

3484145

Edman degradation

PMID:6271171[3]


edit table


Notes

Alleles and Phenotypes

[back to top]


See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

crpR181L

R181L

Suppresses DNA-binding

seeded from UniProt:P0ACJ8

crpR186K

R186K

No modification of DNA-binding

seeded from UniProt:P0ACJ8

crpR186L

R186L

Marginally reduced DNA-binding

seeded from UniProt:P0ACJ8

crpS129A

S129A

Reduced DNA-binding; no modification of cAMP-binding

seeded from UniProt:P0ACJ8

crpR181K

R181K

Suppresses DNA-binding

seeded from UniProt:P0ACJ8

crpS63A

S63A

Enhanced cAMP-binding

seeded from UniProt:P0ACJ8

crpR83L

R83L

Loss of cAMP-binding

seeded from UniProt:P0ACJ8

crpS84A,K

S84A,K

No modification of cAMP-binding

seeded from UniProt:P0ACJ8

crpT128A

T128A

No modification of cAMP-binding

seeded from UniProt:P0ACJ8

crp(del) (Keio:JW5702)

deletion

deletion

PMID:16738554[4]

Shigen

crp::Tn5KAN-I-SceI (FB21127)

Insertion at nt 369 in Minus orientation

PMID:15262929[5]

E. coli Genome Project:FB21127

contains pKD46

crp::Tn5KAN-I-SceI (FB21128)

Insertion at nt 369 in Minus orientation

PMID:15262929[5]

E. coli Genome Project:FB21128

does not contain pKD46

crp(del)::kan

deletion

Biolog:respiration

unable to respire Dextrin

PMID:16095938[6]

crp(del)::kan

deletion

Biolog:respiration

unable to respire N-Acetyl-D-glucosamine

PMID:16095938[6]

crp(del)::kan

deletion

Biolog:respiration

unable to respire L-Fucose

PMID:16095938[6]

crp(del)::kan

deletion

Biolog:respiration

unable to respire D-Galactose

PMID:16095938[6]

crp(del)::kan

deletion

Biolog:respiration

unable to respire Maltose

PMID:16095938[6]

crp(del)::kan

deletion

Biolog:respiration

unable to respire D-Sorbitol

PMID:16095938[6]

crp(del)::kan

deletion

Biolog:respiration

unable to respire Glycerol

PMID:16095938[6]

crp(del)::kan

deletion

Biolog:respiration

unable to respire DL-a-Glycerol

PMID:16095938[6]

crp(del)::kan

deletion

Biolog:respiration

unable to respire D-Galactoniate

PMID:16095938[6]

crp(del)::kan

deletion

Biolog:respiration

unable to respire D-Galacturonate

PMID:16095938[6]

crp(del)::kan

deletion

Biolog:respiration

unable to respire D-Gluconate

PMID:16095938[6]

crp(del)::kan

deletion

Biolog:respiration

unable to respire D-Glucuronate

PMID:16095938[6]

crp(del)::kan

deletion

Biolog:respiration

unable to respire Acetate

PMID:16095938[6]

crp(del)::kan

deletion

Biolog:respiration

unable to respire a-Hydroxybutyrate

PMID:16095938[6]

crp(del)::kan

deletion

Biolog:respiration

unable to respire a-Ketobutyrate

PMID:16095938[6]

crp(del)::kan

deletion

Biolog:respiration

unable to respire a-Ketoglutarate

PMID:16095938[6]

crp(del)::kan

deletion

Biolog:respiration

unable to respire DL-Lactate

PMID:16095938[6]

crp(del)::kan

deletion

Biolog:respiration

unable to respire Succinate

PMID:16095938[6]

crp(del)::kan

deletion

Biolog:respiration

unable to respire Bromosuccinate

PMID:16095938[6]

crp(del)::kan

deletion

Biolog:respiration

unable to respire Glucuronamide

PMID:16095938[6]

crp(del)::kan

deletion

Biolog:respiration

unable to respire L-Alanine

PMID:16095938[6]

crp(del)::kan

deletion

Biolog:respiration

unable to respire L-Asparagine

PMID:16095938[6]

crp(del)::kan

deletion

Biolog:respiration

unable to respire L-Aspartate

PMID:16095938[6]

crp(del)::kan

deletion

Biolog:respiration

unable to respire Glycyl-L-aspartate

PMID:16095938[6]

crp(del)::kan

deletion

Biolog:respiration

unable to respire D-Serine

PMID:16095938[6]

crp(del)::kan

deletion

Biolog:respiration

unable to respire L-Serine

PMID:16095938[6]

crp-3(del)

CGSC:4523

crp-45(del)

CGSC:7790

crp-1

CGSC:7936

crp-2

CGSC:14257

crp-1004

CGSC:17279

crp-39(del)

CGSC:17281

Δcrp-765::kan

PMID:16738554[4]

CGSC:113694

crp(del)

deletion

deletion

Auxotrophies

Mutant unable to grow on MOPS minimal medium containing L-idonate, table 5.

PMID:14973046[7]

experimental strain: CB370

crp(del)

deletion

deletion

Auxotrophies

Mutant unable to grow on MOPS minimal medium containing L-idonate, table 5.

PMID:14973046[7]

experimental strain: CB370

crp(del)

deletion

deletion

Auxotrophies

Mutant unable to grow on MOPS minimal medium containing L-idonate, table 5.

PMID:14973046[7]

experimental strain: CB370

cya (del) crp (del)

Sensitivity to

sensitivity to streptozotocin

PMID:6450313[8]

Strain: CA8445

table 2 and figure 3

crp - relA +

Resistant to

Deficient mutants in relA backgroumd, were found to be resistant to smg (serine+methionine+glycine)

PMID:230407[9]

table 4

cya(del) crp* relA +

Resistant to

crp allele that modified cyclic AMP receptor proteins, which acts independently of cyclic AMP, is resistant to smg (serine+methionine+glycine) in a relA background.

PMID:230407[9]

Experimental strain: BM6163

table 4

crp in mutant strain B1558

Resistant to

Resistance to Beta Lactams

PMID:6344786[10]

Strain: B1558

Table 2

crp -

Resistant to

resistance to Mecillinam

PMID:217866[11]

Strain: A060

Table 2B

crp mutant in strain GC2846

Colony Morphology

Mutatins causes spherical morphology. Wild Type is rod shaped.

PMID:2826407[12]

Strain: GC2846

See figure 1

Salmonella typhimurium LT2 crp-deficient

Resistant to

Partially resistant to 22 antibiotics (including fosfomycin, nalidixic acid, and streptomycin)

PMID:201606[13]

crp in strain NCR-C51

Resistant to

Resistance to Phage T6

PMID:227838[14]

Table 3

crp-403

Resistant to

Resistant to Fosfomycin

PMID:201606[13]

Strain: TA3302

Parent Strain: LT2 Donor Strain: TA2305

crp-401

Spontaneous nonsense mutation

Resistant to

Resistant to Fosfomycin

PMID:201606[13]

Strain: TA3339

Parent Strain: LT2

crp-401

Spontaneous nonsense mutation

Resistant to

Resistant to Streptomycin

PMID:201606[13]

Strain:TA3339

Parent strain: LT2

edit table


Notes

Genetic Interactions

[back to top]



Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
edit table


Notes

Genetic Resources

[back to top]


See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW5702

Plasmid clone

Shigen

PMID:16769691[15]

Status:cloned as old JW

Primer 1:GCCGTGCTTGGCAAACCGCAAAC

Primer 2:CCACGAGTGCCGTAAACGACGAT

4C5

Kohara Phage

Genobase

PMID:3038334[16]

smg-3082::Tn10

Linked marker

CAG12071 = CGSC7444[17]

est. P1 cotransduction: 7% [18]
Synonyms:zhb-3082::Tn10, zhd-3082::Tn10

zhf-3084::Tn10

Linked marker

CAG18456 = CGSC7576[17]

est. P1 cotransduction: 51% [18]
Synonyms:zhe-3084::Tn10nnnCAG18456 also carries cysG0 (CGSC).

edit table


Notes

Accessions in Other Databases

[back to top]


See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10164

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10164

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000160

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:947867

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0162

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0010970

Escherichia coli str. K-12 substr. MG1655

edit table


Notes

Links

[back to top]



<protect>

References

[back to top]


See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ Aiba, H & Krakow, JS (1981) Isolation and characterization of the amino and carboxyl proximal fragments of the adenosine cyclic 3' ,5'-phosphate receptor protein of Escherichia coli. Biochemistry 20 4774-80 PubMed
  4. ↑ 4.0 4.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  5. ↑ 5.0 5.1 Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
  6. ↑ 6.00 6.01 6.02 6.03 6.04 6.05 6.06 6.07 6.08 6.09 6.10 6.11 6.12 6.13 6.14 6.15 6.16 6.17 6.18 6.19 6.20 6.21 6.22 6.23 6.24 6.25 Ito, M et al. (2005) Functional analysis of 1440 Escherichia coli genes using the combination of knock-out library and phenotype microarrays. Metab. Eng. 7 318-27 PubMed
  7. ↑ 7.0 7.1 7.2 Bausch, C et al. (2004) Transcriptional organization and regulation of the L-idonic acid pathway (GntII system) in Escherichia coli. J. Bacteriol. 186 1388-97 PubMed
  8. ↑ Lengeler, J (1980) Characterisation of mutants of Escherichia coli K12, selected by resistance to streptozotocin. Mol. Gen. Genet. 179 49-54 PubMed
  9. ↑ 9.0 9.1 Daniel, J & Danchin, A (1979) Involvement of cyclic AMP and its receptor protein in the sensitivity of Escherichia coli K 12 toward serine: excretion of 2-ketobutyrate, a precursor of isoleucine. Mol. Gen. Genet. 176 343-50 PubMed
  10. ↑ Jaffé, A et al. (1983) Selection and characterization of beta-lactam-resistant Escherichia coli K-12 mutants. Antimicrob. Agents Chemother. 23 622-5 PubMed
  11. ↑ Aono, R et al. (1979) High and selective resistance to mecillinam in adenylate cyclase-deficient or cyclic adenosine 3',5'-monophosphate receptor protein-deficient mutants of Escherichia coli. J. Bacteriol. 137 839-45 PubMed
  12. ↑ D'Ari, R et al. (1988) Cyclic AMP and cell division in Escherichia coli. J. Bacteriol. 170 65-70 PubMed
  13. ↑ 13.0 13.1 13.2 13.3 Alper, MD & Ames, BN (1978) Transport of antibiotics and metabolite analogs by systems under cyclic AMP control: positive selection of Salmonella typhimurium cya and crp mutants. J. Bacteriol. 133 149-57 PubMed
  14. ↑ Alderman, EM et al. (1979) Cyclic adenosine 3',5'-monophosphate regulation of the bacteriophage T6/colicin K receptor in Escherichia coli. J. Bacteriol. 140 369-76 PubMed
  15. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  16. ↑ Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  17. ↑ 17.0 17.1 CGSC: The Coli Genetics Stock Center
  18. ↑ 18.0 18.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

Categories

Retrieved from "https://ecoliwiki.org/colipedia/index.php?title=crp:Gene&oldid=1490570"