smg:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

smg

Mnemonic

fms backwards, g>f

Synonyms

ECK3271, b3284, JW3245[1], JW3245

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

73.93 minutes, 73.93 minutes 

MG1655: 3430486..3430013
<gbrowseImage> name=NC_000913:3430013..3430486 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 3360369..3359896
<gbrowseImage> name=NC_012967:3359896..3360369 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 3317170..3317643
<gbrowseImage> name=NC_012759:3317170..3317643 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 4207952..4208425
<gbrowseImage> name=NC_007779:4207952..4208425 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 3528231..3527758
<gbrowseImage> name=NC_010473:3527758..3528231 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

Δsmg (Keio:JW3245)

deletion

deletion

PMID:16738554[2]

Shigen
CGSC10450[3]

smg::Tn5KAN-I-SceI (FB21096)

Insertion at nt 305 in Plus orientation

PMID:15262929[4]

E. coli Genome Project:FB21096

does not contain pKD46

smg::Tn5KAN-I-SceI (FB21097)

Insertion at nt 393 in Minus orientation

PMID:15262929[4]

E. coli Genome Project:FB21097

contains pKD46

smg-3082::Tn10

Insertion at 3,430,197 bp in MG1655 (NC_000913)

adapted from Nichols et al.[5]

CAG12071 = CGSC7444[3]

Synonyms: zhb-3082::Tn10, zhd-3082::Tn10

smg-3082::Tn10

PMID:2540407[6] PMID:9829956[7]

CGSC:40238

smg-3170::Tn10kan

PMID:2540407[6]

CGSC:40242

Δsmg-723::kan

PMID:16738554[2]

CGSC:102972


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW3245

Plasmid clone

Shigen

PMID:16769691[8]

Status:Clone OK

Primer 1:GCCTTCGACGTACTAATGTATTT

Primer 2:CCATGCAGCATACCTTCATTCAC

acrF3083::Tn10

Linked marker

CAG12075 = CGSC7447[3]

est. P1 cotransduction: 58% [5]
Synonyms:zhd-3083::Tn10, zhe-3083::Tn10

zhf-3084::Tn10

Linked marker

CAG18456 = CGSC7576[3]

est. P1 cotransduction: 1% [5]
Synonyms:zhe-3084::Tn10nnnCAG18456 also carries cysG0 (CGSC).

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG11605

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG11605

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001553

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:947781

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1562

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0010770

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  3. ↑ 3.0 3.1 3.2 3.3 CGSC: The Coli Genetics Stock Center
  4. ↑ 4.0 4.1 Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
  5. ↑ 5.0 5.1 5.2 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  6. ↑ 6.0 6.1 Singer, M et al. (1989) A collection of strains containing genetically linked alternating antibiotic resistance elements for genetic mapping of Escherichia coli. Microbiol. Rev. 53 1-24 PubMed
  7. ↑ Nichols, BP et al. (1998) Sequence analysis of Tn10 insertion sites in a collection of Escherichia coli strains used for genetic mapping and strain construction. J. Bacteriol. 180 6408-11 PubMed
  8. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed

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