crp:On One Page

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Quickview | Gene | Product(s) | Expression| Evolution | References |

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Standard Name

crp

Gene Synonym(s)

ECK3345, b3357, JW5702, cap, csm, gurB, G51[1][2]

Product Desc.

CRP transcriptional dual regulator[2][3];

Component of CRP transcriptional dual regulator[2][3]; CRP-cAMP transcriptional dual regulator[3]

cAMP receptor protein[4]

Product Synonyms(s)

DNA-binding transcriptional dual regulator[1], Crp[2][1], GurB[2][1], Csm[2][1], Cap[2][1], B3357[2][1] , cap, csm, ECK3345, JW5702, b3357

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): crp[2], OP00160

Regulation/Activity
Quick Links

porteco.png EcoCyc.gif regulondb.jpg

dnadisplay.png proteindisplay.png   pubmed.jpg   textpresso.jpg  

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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

crp

Mnemonic

cAMP receptor protein

Synonyms

ECK3345, b3357, JW5702, cap, csm, gurB, G51[1][2]

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

75.09 minutes, 75.09 minutes 

MG1655: 3484142..3484774
<gbrowseImage> name=NC_000913:3484142..3484774 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 3414025..3414657
<gbrowseImage> name=NC_012967:3414025..3414657 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 3371299..3371931
<gbrowseImage> name=NC_012759:3371299..3371931 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 4154296..4153664
<gbrowseImage> name=NC_007779:4153664..4154296 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 3581887..3582519
<gbrowseImage> name=NC_010473:3581887..3582519 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

3484145

Edman degradation

PMID:6271171


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

crpR181L

R181L

Suppresses DNA-binding

seeded from UniProt:P0ACJ8

crpR186K

R186K

No modification of DNA-binding

seeded from UniProt:P0ACJ8

crpR186L

R186L

Marginally reduced DNA-binding

seeded from UniProt:P0ACJ8

crpS129A

S129A

Reduced DNA-binding; no modification of cAMP-binding

seeded from UniProt:P0ACJ8

crpR181K

R181K

Suppresses DNA-binding

seeded from UniProt:P0ACJ8

crpS63A

S63A

Enhanced cAMP-binding

seeded from UniProt:P0ACJ8

crpR83L

R83L

Loss of cAMP-binding

seeded from UniProt:P0ACJ8

crpS84A,K

S84A,K

No modification of cAMP-binding

seeded from UniProt:P0ACJ8

crpT128A

T128A

No modification of cAMP-binding

seeded from UniProt:P0ACJ8

crp(del) (Keio:JW5702)

deletion

deletion

PMID:16738554

Shigen

crp::Tn5KAN-I-SceI (FB21127)

Insertion at nt 369 in Minus orientation

PMID:15262929

E. coli Genome Project:FB21127

contains pKD46

crp::Tn5KAN-I-SceI (FB21128)

Insertion at nt 369 in Minus orientation

PMID:15262929

E. coli Genome Project:FB21128

does not contain pKD46

crp(del)::kan

deletion

Biolog:respiration

unable to respire Dextrin

PMID:16095938

crp(del)::kan

deletion

Biolog:respiration

unable to respire N-Acetyl-D-glucosamine

PMID:16095938

crp(del)::kan

deletion

Biolog:respiration

unable to respire L-Fucose

PMID:16095938

crp(del)::kan

deletion

Biolog:respiration

unable to respire D-Galactose

PMID:16095938

crp(del)::kan

deletion

Biolog:respiration

unable to respire Maltose

PMID:16095938

crp(del)::kan

deletion

Biolog:respiration

unable to respire D-Sorbitol

PMID:16095938

crp(del)::kan

deletion

Biolog:respiration

unable to respire Glycerol

PMID:16095938

crp(del)::kan

deletion

Biolog:respiration

unable to respire DL-a-Glycerol

PMID:16095938

crp(del)::kan

deletion

Biolog:respiration

unable to respire D-Galactoniate

PMID:16095938

crp(del)::kan

deletion

Biolog:respiration

unable to respire D-Galacturonate

PMID:16095938

crp(del)::kan

deletion

Biolog:respiration

unable to respire D-Gluconate

PMID:16095938

crp(del)::kan

deletion

Biolog:respiration

unable to respire D-Glucuronate

PMID:16095938

crp(del)::kan

deletion

Biolog:respiration

unable to respire Acetate

PMID:16095938

crp(del)::kan

deletion

Biolog:respiration

unable to respire a-Hydroxybutyrate

PMID:16095938

crp(del)::kan

deletion

Biolog:respiration

unable to respire a-Ketobutyrate

PMID:16095938

crp(del)::kan

deletion

Biolog:respiration

unable to respire a-Ketoglutarate

PMID:16095938

crp(del)::kan

deletion

Biolog:respiration

unable to respire DL-Lactate

PMID:16095938

crp(del)::kan

deletion

Biolog:respiration

unable to respire Succinate

PMID:16095938

crp(del)::kan

deletion

Biolog:respiration

unable to respire Bromosuccinate

PMID:16095938

crp(del)::kan

deletion

Biolog:respiration

unable to respire Glucuronamide

PMID:16095938

crp(del)::kan

deletion

Biolog:respiration

unable to respire L-Alanine

PMID:16095938

crp(del)::kan

deletion

Biolog:respiration

unable to respire L-Asparagine

PMID:16095938

crp(del)::kan

deletion

Biolog:respiration

unable to respire L-Aspartate

PMID:16095938

crp(del)::kan

deletion

Biolog:respiration

unable to respire Glycyl-L-aspartate

PMID:16095938

crp(del)::kan

deletion

Biolog:respiration

unable to respire D-Serine

PMID:16095938

crp(del)::kan

deletion

Biolog:respiration

unable to respire L-Serine

PMID:16095938

crp-3(del)

CGSC:4523

crp-45(del)

CGSC:7790

crp-1

CGSC:7936

crp-2

CGSC:14257

crp-1004

CGSC:17279

crp-39(del)

CGSC:17281

Δcrp-765::kan

PMID:16738554

CGSC:113694

crp(del)

deletion

deletion

Auxotrophies

Mutant unable to grow on MOPS minimal medium containing L-idonate, table 5.

PMID:14973046

experimental strain: CB370

crp(del)

deletion

deletion

Auxotrophies

Mutant unable to grow on MOPS minimal medium containing L-idonate, table 5.

PMID:14973046

experimental strain: CB370

crp(del)

deletion

deletion

Auxotrophies

Mutant unable to grow on MOPS minimal medium containing L-idonate, table 5.

PMID:14973046

experimental strain: CB370

cya (del) crp (del)

Sensitivity to

sensitivity to streptozotocin

PMID:6450313

Strain: CA8445

table 2 and figure 3

crp - relA +

Resistant to

Deficient mutants in relA backgroumd, were found to be resistant to smg (serine+methionine+glycine)

PMID:230407

table 4

cya(del) crp* relA +

Resistant to

crp allele that modified cyclic AMP receptor proteins, which acts independently of cyclic AMP, is resistant to smg (serine+methionine+glycine) in a relA background.

PMID:230407

Experimental strain: BM6163

table 4

crp in mutant strain B1558

Resistant to

Resistance to Beta Lactams

PMID:6344786

Strain: B1558

Table 2

crp -

Resistant to

resistance to Mecillinam

PMID:217866

Strain: A060

Table 2B

crp mutant in strain GC2846

Colony Morphology

Mutatins causes spherical morphology. Wild Type is rod shaped.

PMID:2826407

Strain: GC2846

See figure 1

Salmonella typhimurium LT2 crp-deficient

Resistant to

Partially resistant to 22 antibiotics (including fosfomycin, nalidixic acid, and streptomycin)

PMID:201606

crp in strain NCR-C51

Resistant to

Resistance to Phage T6

PMID:227838

Table 3

crp-403

Resistant to

Resistant to Fosfomycin

PMID:201606

Strain: TA3302

Parent Strain: LT2 Donor Strain: TA2305

crp-401

Spontaneous nonsense mutation

Resistant to

Resistant to Fosfomycin

PMID:201606

Strain: TA3339

Parent Strain: LT2

crp-401

Spontaneous nonsense mutation

Resistant to

Resistant to Streptomycin

PMID:201606

Strain:TA3339

Parent strain: LT2

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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW5702

Plasmid clone

Shigen

PMID:16769691

Status:cloned as old JW

Primer 1:GCCGTGCTTGGCAAACCGCAAAC

Primer 2:CCACGAGTGCCGTAAACGACGAT

4C5

Kohara Phage

Genobase

PMID:3038334

smg-3082::Tn10

Linked marker

CAG12071 = CGSC7444[5]

est. P1 cotransduction: 7% [6]
Synonyms:zhb-3082::Tn10, zhd-3082::Tn10

zhf-3084::Tn10

Linked marker

CAG18456 = CGSC7576[5]

est. P1 cotransduction: 51% [6]
Synonyms:zhe-3084::Tn10nnnCAG18456 also carries cysG0 (CGSC).

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10164

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10164

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000160

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:947867

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0162

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0010970

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

Crp

Synonyms

DNA-binding transcriptional dual regulator[1], Crp[2][1], GurB[2][1], Csm[2][1], Cap[2][1], B3357[2][1] , cap, csm, ECK3345, JW5702, b3357

Product description

CRP transcriptional dual regulator[2][3];

Component of CRP transcriptional dual regulator[2][3]; CRP-cAMP transcriptional dual regulator[3]

cAMP receptor protein[4]

EC number (for enzymes)


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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status

GO:0000166

nucleotide binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0547

F

Seeded from EcoCyc (v14.0)

complete

GO:0003677

DNA binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR012318

F

Seeded from EcoCyc (v14.0)

complete

GO:0003677

DNA binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0238

F

Seeded from EcoCyc (v14.0)

complete

GO:0003700

transcription factor activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001808

F

Seeded from EcoCyc (v14.0)

complete

GO:0045991

positive regulation of transcription by carbon catabolites

P

Missing: evidence, reference

GO:0003700

transcription factor activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR018335

F

Seeded from EcoCyc (v14.0)

complete

GO:0005515

protein binding

PMID:15690043

IPI: Inferred from Physical Interaction

UniProtKB:P0A7K2

F

Seeded from EcoCyc (v14.0)

complete

GO:0005622

intracellular

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001808

C

Seeded from EcoCyc (v14.0)

complete

GO:0005622

intracellular

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR018335

C

Seeded from EcoCyc (v14.0)

complete

GO:0006350

transcription

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0804

P

Seeded from EcoCyc (v14.0)

complete

GO:0006350

transcription

PMID:10760178

IDA: Inferred from Direct Assay

P

Seeded from EcoCyc (v14.0)

complete

GO:0006350

transcription

PMID:11742992

IDA: Inferred from Direct Assay

P

Seeded from EcoCyc (v14.0)

complete

GO:0006350

transcription

PMID:12923087

IDA: Inferred from Direct Assay

P

Seeded from EcoCyc (v14.0)

complete

GO:0006350

transcription

PMID:12923087

IMP: Inferred from Mutant Phenotype

P

Seeded from EcoCyc (v14.0)

complete

GO:0006350

transcription

PMID:15520470

IEP: Inferred from Expression Pattern

P

Seeded from EcoCyc (v14.0)

complete

GO:0006350

transcription

PMID:16166533

IDA: Inferred from Direct Assay

P

Seeded from EcoCyc (v14.0)

complete

GO:0006350

transcription

PMID:6343617

ISS: Inferred from Sequence or Structural Similarity

Swiss-Prot:P0A9E5

P

Seeded from EcoCyc (v14.0)

complete

GO:0006350

transcription

PMID:7551052

IDA: Inferred from Direct Assay

P

Seeded from EcoCyc (v14.0)

complete

GO:0006350

transcription

PMID:7551052

IEP: Inferred from Expression Pattern

P

Seeded from EcoCyc (v14.0)

complete

GO:0006350

transcription

PMID:8604346

IMP: Inferred from Mutant Phenotype

P

Seeded from EcoCyc (v14.0)

complete

GO:0006355

regulation of transcription, DNA-dependent

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001808

P

Seeded from EcoCyc (v14.0)

complete

GO:0006355

regulation of transcription, DNA-dependent

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR018335

P

Seeded from EcoCyc (v14.0)

complete

GO:0016563

transcription activator activity

PMID:10760178

IDA: Inferred from Direct Assay

F

Seeded from EcoCyc (v14.0)

complete

GO:0016563

transcription activator activity

PMID:11742992

IDA: Inferred from Direct Assay

F

Seeded from EcoCyc (v14.0)

complete

GO:0016563

transcription activator activity

PMID:12923087

IDA: Inferred from Direct Assay

F

Seeded from EcoCyc (v14.0)

complete

GO:0016563

transcription activator activity

PMID:12923087

IMP: Inferred from Mutant Phenotype

F

Seeded from EcoCyc (v14.0)

complete

GO:0016563

transcription activator activity

PMID:15520470

IEP: Inferred from Expression Pattern

F

Seeded from EcoCyc (v14.0)

complete

GO:0016563

transcription activator activity

PMID:16166533

IDA: Inferred from Direct Assay

F

Seeded from EcoCyc (v14.0)

complete

GO:0016563

transcription activator activity

PMID:6343617

ISS: Inferred from Sequence or Structural Similarity

Swiss-Prot:P0A9E5

F

Seeded from EcoCyc (v14.0)

complete

GO:0016563

transcription activator activity

PMID:7551052

IDA: Inferred from Direct Assay

F

Seeded from EcoCyc (v14.0)

complete

GO:0016563

transcription activator activity

PMID:7551052

IEP: Inferred from Expression Pattern

F

Seeded from EcoCyc (v14.0)

complete

GO:0016563

transcription activator activity

PMID:8604346

IMP: Inferred from Mutant Phenotype

F

Seeded from EcoCyc (v14.0)

complete

GO:0016564

transcription repressor activity

PMID:10760178

IDA: Inferred from Direct Assay

F

Seeded from EcoCyc (v14.0)

complete

GO:0016564

transcription repressor activity

PMID:11742992

IDA: Inferred from Direct Assay

F

Seeded from EcoCyc (v14.0)

complete

GO:0016564

transcription repressor activity

PMID:12923087

IDA: Inferred from Direct Assay

F

Seeded from EcoCyc (v14.0)

complete

GO:0016564

transcription repressor activity

PMID:12923087

IMP: Inferred from Mutant Phenotype

F

Seeded from EcoCyc (v14.0)

complete

GO:0016564

transcription repressor activity

PMID:15520470

IEP: Inferred from Expression Pattern

F

Seeded from EcoCyc (v14.0)

complete

GO:0016564

transcription repressor activity

PMID:16166533

IDA: Inferred from Direct Assay

F

Seeded from EcoCyc (v14.0)

complete

GO:0016564

transcription repressor activity

PMID:6343617

ISS: Inferred from Sequence or Structural Similarity

Swiss-Prot:P0A9E5

F

Seeded from EcoCyc (v14.0)

complete

GO:0016564

transcription repressor activity

PMID:7551052

IDA: Inferred from Direct Assay

F

Seeded from EcoCyc (v14.0)

complete

GO:0016564

transcription repressor activity

PMID:7551052

IEP: Inferred from Expression Pattern

F

Seeded from EcoCyc (v14.0)

complete

GO:0016564

transcription repressor activity

PMID:8604346

IMP: Inferred from Mutant Phenotype

F

Seeded from EcoCyc (v14.0)

complete

GO:0030552

cAMP binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0116

F

Seeded from EcoCyc (v14.0)

complete

GO:0045449

regulation of transcription

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR012318

P

Seeded from EcoCyc (v14.0)

complete

GO:0045449

regulation of transcription

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0805

P

Seeded from EcoCyc (v14.0)

complete

GO:0045893

positive regulation of transcription, DNA-dependent

PMID:15520470

IDA: Inferred from Direct Assay

P

replacing obsolete GO:0016563

complete

GO:0045892

negative regulation of transcription, DNA-dependent

PMID:15520470

IDA: Inferred from Direct Assay

P

replacing obsolete GO:0016564

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

Subunits of CRP transcriptional dual regulator

homodimer

Protein

rplC

PMID:15690043

Experiment(s):EBI-885165

Protein

rpsE

PMID:15690043

Experiment(s):EBI-885165

Protein

rpsJ

PMID:15690043

Experiment(s):EBI-885165

Protein

rplL

PMID:15690043

Experiment(s):EBI-891949

Protein

Subunits of CRP-cAMP transcriptional dual regulator

could be indirect

Small Molecule

cAMP


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Notes

Binding sites for CAP-cAMP have been localized experimentally by ChIP-Chip experiments[7].

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MVLGKPQTDP TLEWFLSHCH IHKYPSKSTL IHQGEKAETL YYIVKGSVAV LIKDEEGKEM
ILSYLNQGDF IGELGLFEEG QERSAWVRAK TACEVAEISY KKFRQLIQVN PDILMRLSAQ
MARRLQVTSE KVGNLAFLDV TGRIAQTLLN LAKQPDAMTH PDGMQIKITR QEIGQIVGCS
RETVGRILKM LEDQNLISAH GKTIVVYGTR
Length

210

Mol. Wt

23.64 kDa

pI

8.4 (calculated)

Extinction coefficient

19,940 - 20,315 (calc based on 6 Y, 2 W, and 3 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

21..112

PF00027 Cyclic nucleotide-binding domain

PMID:19920124

Domain

166..197

PF00325 Bacterial regulatory proteins, crp family

PMID:19920124

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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=crp taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16131236

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:947867

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0010970

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P0ACJ8

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10164

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10164

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:947867

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000160

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0162

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

Protein

E. coli K-12 MC4100

1.98E+03

molecules/cell

  • Medium: median data from rich and minimal media
  • Temperature (°C): 37
  • Doubling time (min): Growth Rate(min):
  • Growth phase: other:

emPAI

PMID:18304323

Protein

E. coli K-12 MG1655

9767

molecules/cell/generation

  • Medium: MOPS Complete
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

3903

molecules/cell/generation

  • Medium: MOPS Minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

5159

molecules/cell/generation

  • Medium: MOPS Complete without Methionine
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

crp

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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:3484122..3484162 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GEO Profiles:b3357 (EcoliWiki Page)

NCBI GEO profiles for crp

microarray

GenExpDB:b3357 (EcoliWiki Page)

Summary of data for crp from multiple microarray studies

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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes

GFP Fusion

Intergenic region (3483367..3483829) fused to gfpmut2.

OpenBioSystems

GFP fusion described in Zaslaver, et al.
Plate:109-AZ02; Well:A6[8]

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Notes

Accessions Related to crp Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10164

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0162

Escherichia coli str. K-12 substr. MG1655

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b3357

EcoGene

EcoGene:EG10164

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000160

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0010970

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism Homologs (Statistics) Comments

Xenopus tropicalis

  • ENSXETP00000000047 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Shigella flexneri

CRP

From SHIGELLACYC

E. coli O157

CRP

From ECOO157CYC

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Do-It-Yourself Web Tools

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Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

Pfam (EcoliWiki Page)

PF00325 Bacterial regulatory proteins, crp family

Pfam (EcoliWiki Page)

PF00027 Cyclic nucleotide-binding domain

Superfamily (EcoliWiki Page)

SUPERFAMILY:46785

Superfamily (EcoliWiki Page)

SUPERFAMILY:51206

Panther (EcoliWiki Page)

PTHR10217:SF24

EcoCyc

EcoCyc:EG10164

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10164

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000160

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0162

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0010970

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 2.13 2.14 2.15 2.16 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 3.2 3.3 3.4 3.5 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ 4.0 4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 CGSC: The Coli Genetics Stock Center
  6. ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  7. ↑ Grainger, DC et al. (2005) Studies of the distribution of Escherichia coli cAMP-receptor protein and RNA polymerase along the E. coli chromosome. Proc. Natl. Acad. Sci. U.S.A. 102 17693-8 PubMed
  8. ↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

Categories

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