crp:Gene Product(s)

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Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

Crp

Synonyms

DNA-binding transcriptional dual regulator[1], Crp[2][1], GurB[2][1], Csm[2][1], Cap[2][1], B3357[2][1] , cap, csm, ECK3345, JW5702, b3357

Product description

CRP transcriptional dual regulator[2][3];

Component of CRP transcriptional dual regulator[2][3]; CRP-cAMP transcriptional dual regulator[3]

cAMP receptor protein[4]

EC number (for enzymes)


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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0000166

nucleotide binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0547

F

Seeded from EcoCyc (v14.0)

complete

GO:0003677

DNA binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR012318

F

Seeded from EcoCyc (v14.0)

complete

GO:0003677

DNA binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0238

F

Seeded from EcoCyc (v14.0)

complete

GO:0003700

transcription factor activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001808

F

Seeded from EcoCyc (v14.0)

complete

GO:0045991

positive regulation of transcription by carbon catabolites

P

Missing: evidence, reference

GO:0003700

transcription factor activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR018335

F

Seeded from EcoCyc (v14.0)

complete

GO:0005515

protein binding

PMID:15690043[5]

IPI: Inferred from Physical Interaction

UniProtKB:P0A7K2

F

Seeded from EcoCyc (v14.0)

complete

GO:0005622

intracellular

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001808

C

Seeded from EcoCyc (v14.0)

complete

GO:0005622

intracellular

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR018335

C

Seeded from EcoCyc (v14.0)

complete

GO:0006350

transcription

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0804

P

Seeded from EcoCyc (v14.0)

complete

GO:0006350

transcription

PMID:10760178[6]

IDA: Inferred from Direct Assay

P

Seeded from EcoCyc (v14.0)

complete

GO:0006350

transcription

PMID:11742992[7]

IDA: Inferred from Direct Assay

P

Seeded from EcoCyc (v14.0)

complete

GO:0006350

transcription

PMID:12923087[8]

IDA: Inferred from Direct Assay

P

Seeded from EcoCyc (v14.0)

complete

GO:0006350

transcription

PMID:12923087[8]

IMP: Inferred from Mutant Phenotype

P

Seeded from EcoCyc (v14.0)

complete

GO:0006350

transcription

PMID:15520470[9]

IEP: Inferred from Expression Pattern

P

Seeded from EcoCyc (v14.0)

complete

GO:0006350

transcription

PMID:16166533[10]

IDA: Inferred from Direct Assay

P

Seeded from EcoCyc (v14.0)

complete

GO:0006350

transcription

PMID:6343617[11]

ISS: Inferred from Sequence or Structural Similarity

Swiss-Prot:P0A9E5

P

Seeded from EcoCyc (v14.0)

complete

GO:0006350

transcription

PMID:7551052[12]

IDA: Inferred from Direct Assay

P

Seeded from EcoCyc (v14.0)

complete

GO:0006350

transcription

PMID:7551052[12]

IEP: Inferred from Expression Pattern

P

Seeded from EcoCyc (v14.0)

complete

GO:0006350

transcription

PMID:8604346[13]

IMP: Inferred from Mutant Phenotype

P

Seeded from EcoCyc (v14.0)

complete

GO:0006355

regulation of transcription, DNA-dependent

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001808

P

Seeded from EcoCyc (v14.0)

complete

GO:0006355

regulation of transcription, DNA-dependent

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR018335

P

Seeded from EcoCyc (v14.0)

complete

GO:0016563

transcription activator activity

PMID:10760178[6]

IDA: Inferred from Direct Assay

F

Seeded from EcoCyc (v14.0)

complete

GO:0016563

transcription activator activity

PMID:11742992[7]

IDA: Inferred from Direct Assay

F

Seeded from EcoCyc (v14.0)

complete

GO:0016563

transcription activator activity

PMID:12923087[8]

IDA: Inferred from Direct Assay

F

Seeded from EcoCyc (v14.0)

complete

GO:0016563

transcription activator activity

PMID:12923087[8]

IMP: Inferred from Mutant Phenotype

F

Seeded from EcoCyc (v14.0)

complete

GO:0016563

transcription activator activity

PMID:15520470[9]

IEP: Inferred from Expression Pattern

F

Seeded from EcoCyc (v14.0)

complete

GO:0016563

transcription activator activity

PMID:16166533[10]

IDA: Inferred from Direct Assay

F

Seeded from EcoCyc (v14.0)

complete

GO:0016563

transcription activator activity

PMID:6343617[11]

ISS: Inferred from Sequence or Structural Similarity

Swiss-Prot:P0A9E5

F

Seeded from EcoCyc (v14.0)

complete

GO:0016563

transcription activator activity

PMID:7551052[12]

IDA: Inferred from Direct Assay

F

Seeded from EcoCyc (v14.0)

complete

GO:0016563

transcription activator activity

PMID:7551052[12]

IEP: Inferred from Expression Pattern

F

Seeded from EcoCyc (v14.0)

complete

GO:0016563

transcription activator activity

PMID:8604346[13]

IMP: Inferred from Mutant Phenotype

F

Seeded from EcoCyc (v14.0)

complete

GO:0016564

transcription repressor activity

PMID:10760178[6]

IDA: Inferred from Direct Assay

F

Seeded from EcoCyc (v14.0)

complete

GO:0016564

transcription repressor activity

PMID:11742992[7]

IDA: Inferred from Direct Assay

F

Seeded from EcoCyc (v14.0)

complete

GO:0016564

transcription repressor activity

PMID:12923087[8]

IDA: Inferred from Direct Assay

F

Seeded from EcoCyc (v14.0)

complete

GO:0016564

transcription repressor activity

PMID:12923087[8]

IMP: Inferred from Mutant Phenotype

F

Seeded from EcoCyc (v14.0)

complete

GO:0016564

transcription repressor activity

PMID:15520470[9]

IEP: Inferred from Expression Pattern

F

Seeded from EcoCyc (v14.0)

complete

GO:0016564

transcription repressor activity

PMID:16166533[10]

IDA: Inferred from Direct Assay

F

Seeded from EcoCyc (v14.0)

complete

GO:0016564

transcription repressor activity

PMID:6343617[11]

ISS: Inferred from Sequence or Structural Similarity

Swiss-Prot:P0A9E5

F

Seeded from EcoCyc (v14.0)

complete

GO:0016564

transcription repressor activity

PMID:7551052[12]

IDA: Inferred from Direct Assay

F

Seeded from EcoCyc (v14.0)

complete

GO:0016564

transcription repressor activity

PMID:7551052[12]

IEP: Inferred from Expression Pattern

F

Seeded from EcoCyc (v14.0)

complete

GO:0016564

transcription repressor activity

PMID:8604346[13]

IMP: Inferred from Mutant Phenotype

F

Seeded from EcoCyc (v14.0)

complete

GO:0030552

cAMP binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0116

F

Seeded from EcoCyc (v14.0)

complete

GO:0045449

regulation of transcription

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR012318

P

Seeded from EcoCyc (v14.0)

complete

GO:0045449

regulation of transcription

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0805

P

Seeded from EcoCyc (v14.0)

complete

GO:0045893

positive regulation of transcription, DNA-dependent

PMID:15520470[9]

IDA: Inferred from Direct Assay

P

replacing obsoleteGO:0016563

complete

GO:0045892

negative regulation of transcription, DNA-dependent

PMID:15520470[9]

IDA: Inferred from Direct Assay

P

replacing obsoleteGO:0016564

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

Subunits of CRP transcriptional dual regulator

homodimer

Protein

rplC

PMID:15690043[5]

Experiment(s):EBI-885165

Protein

rpsE

PMID:15690043[5]

Experiment(s):EBI-885165

Protein

rpsJ

PMID:15690043[5]

Experiment(s):EBI-885165

Protein

rplL

PMID:15690043[5]

Experiment(s):EBI-891949

Protein

Subunits of CRP-cAMP transcriptional dual regulator

could be indirect

Small Molecule

cAMP


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Notes

Binding sites for CAP-cAMP have been localized experimentally by ChIP-Chip experiments[14].

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MVLGKPQTDP TLEWFLSHCH IHKYPSKSTL IHQGEKAETL YYIVKGSVAV LIKDEEGKEM
ILSYLNQGDF IGELGLFEEG QERSAWVRAK TACEVAEISY KKFRQLIQVN PDILMRLSAQ
MARRLQVTSE KVGNLAFLDV TGRIAQTLLN LAKQPDAMTH PDGMQIKITR QEIGQIVGCS
RETVGRILKM LEDQNLISAH GKTIVVYGTR
Length

210

Mol. Wt

23.64 kDa

pI

8.4 (calculated)

Extinction coefficient

19,940 - 20,315 (calc based on 6 Y, 2 W, and 3 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

21..112

PF00027 Cyclic nucleotide-binding domain

PMID:19920124[15]

Domain

166..197

PF00325 Bacterial regulatory proteins, crp family

PMID:19920124[15]

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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=crp taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16131236

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:947867

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0010970

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P0ACJ8

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10164

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10164

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:947867

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000160

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0162

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 1.4 1.5 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 2.4 2.5 2.6 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 3.2 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 5.2 5.3 5.4 Butland, G et al. (2005) Interaction network containing conserved and essential protein complexes in Escherichia coli. Nature 433 531-7 PubMed
  6. ↑ 6.0 6.1 6.2 Belyaeva, TA et al. (2000) Transcription activation at the Escherichia coli melAB promoter: the role of MelR and the cyclic AMP receptor protein. Mol. Microbiol. 36 211-22 PubMed
  7. ↑ 7.0 7.1 7.2 Wade, JT et al. (2001) A simple mechanism for co-dependence on two activators at an Escherichia coli promoter. EMBO J. 20 7160-7 PubMed
  8. ↑ 8.0 8.1 8.2 8.3 8.4 8.5 Beatty, CM et al. (2003) Cyclic AMP receptor protein-dependent activation of the Escherichia coli acsP2 promoter by a synergistic class III mechanism. J. Bacteriol. 185 5148-57 PubMed
  9. ↑ 9.0 9.1 9.2 9.3 9.4 Zheng, D et al. (2004) Identification of the CRP regulon using in vitro and in vivo transcriptional profiling. Nucleic Acids Res. 32 5874-93 PubMed
  10. ↑ 10.0 10.1 10.2 Wickstrum, JR et al. (2005) Cyclic AMP receptor protein and RhaR synergistically activate transcription from the L-rhamnose-responsive rhaSR promoter in Escherichia coli. J. Bacteriol. 187 6708-18 PubMed
  11. ↑ 11.0 11.1 11.2 Shaw, DJ et al. (1983) Homology between CAP and Fnr, a regulator of anaerobic respiration in Escherichia coli. J. Mol. Biol. 166 241-7 PubMed
  12. ↑ 12.0 12.1 12.2 12.3 12.4 12.5 Ramseier, TM & Saier, MH Jr (1995) cAMP-cAMP receptor protein complex: five binding sites in the control region of the Escherichia coli mannitol operon. Microbiology (Reading, Engl.) 141 ( Pt 8) 1901-7 PubMed
  13. ↑ 13.0 13.1 13.2 Williams, RM et al. (1996) Orientation of functional activating regions in the Escherichia coli CRP protein during transcription activation at class II promoters. Nucleic Acids Res. 24 1112-8 PubMed
  14. ↑ Grainger, DC et al. (2005) Studies of the distribution of Escherichia coli cAMP-receptor protein and RNA polymerase along the E. coli chromosome. Proc. Natl. Acad. Sci. U.S.A. 102 17693-8 PubMed
  15. ↑ 15.0 15.1 Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed

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