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On One Page

Quickview |

Gene |

Product(s) |

Expression|

Evolution |

References |

Quickview

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Standard Name	rep
Gene Synonym(s)	ECK3770, b3778, JW5604, dasC, mbrA, mmrA, dasC?^[1]^[2], mmrA?
Product Desc.	rep^[2]^[3]; Component of rep helicase, a single-stranded DNA dependent ATPase^[3] ATP-dependent DNA helicase Rep, involved in DNA replication; ssDNA-dependent ATPase^[4]
Product Synonyms(s)	DNA helicase and single-stranded DNA-dependent ATPase^[1], B3778^[2]^[1], MmrA^[2]^[1], MbrA^[2]^[1], DasC^[2]^[1], Rep^[2]^[1] , dasC, ECK3770, JW5604, mbrA, mmrA?, b3778
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): rep^[2]
Regulation/Activity
Quick Links
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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	rep
Mnemonic	Replicase
Synonyms	ECK3770, b3778, JW5604, dasC, mbrA, mmrA, dasC?^[1]^[2], mmrA?
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	85.32 minutes	MG1655: 3958700..3960721 <gbrowseImage> name=NC_000913:3958700..3960721 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 3922479..3924500 <gbrowseImage> name=NC_012967:3922479..3924500 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 3848369..3850390 <gbrowseImage> name=NC_012759:3848369..3850390 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 3676004..3673983 <gbrowseImage> name=NC_007779:3673983..3676004 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 4057620..4059641 <gbrowseImage> name=NC_010473:4057620..4059641 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
Coding Start (SO:0000323)	MG1655	3958700	Edman degradation	PMID:2999067
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Type	Phenotype: Description	Reference	Availability	Comments
rep(del) (Keio:JW5604)	deletion	deletion			PMID:16738554	Shigen
rep::Tn5KAN-I-SceI (FB21451)	Insertion at nt 508 in Minus orientation				PMID:15262929	E. coli Genome Project:FB21451	contains pKD46
Δrep::kan	deletion		Biolog:respiration	unable to respire Acetate	PMID:16095938
Δrep::kan	deletion		Biolog:respiration	unable to respire a-Hydroxybutyrate	PMID:16095938
Δrep::kan	deletion		Biolog:respiration	unable to respire a-Ketobutyrate	PMID:16095938
Δrep::kan	deletion		Biolog:respiration	unable to respire a-Ketoglutarate	PMID:16095938
Δrep::kan	deletion		Biolog:respiration	unable to respire Succinate	PMID:16095938
Δrep::kan	deletion		Biolog:respiration	unable to respire Bromosuccinate	PMID:16095938
Δrep::kan	deletion		Biolog:respiration	unable to respire L-Alanine	PMID:16095938
Δrep::kan	deletion		Biolog:respiration	unable to respire L-Asparagine	PMID:16095938
Δrep::kan	deletion		Biolog:respiration	unable to respire L-Aspartate	PMID:16095938
Δrep::kan	deletion		Biolog:respiration	unable to respire L-Serine	PMID:16095938
rep-5						CGSC:7013
rep-3					PMID:383240	CGSC:9794
rep-142(ts)				temperature sensitive		CGSC:90737
[rep-71]					PMID:6286604	CGSC:90823	null allele
rep-729(del)::kan					PMID:16738554	CGSC:113537
rep in MDS42	deletion		Sensitivity to	increased sensitivity to Bicyclomycin	PMID:21183718		Fig. 2
mbrA4			Growth Phenotype	no growth in the first few hours at non-permissive conditions but eventually resumed growth, after exposed to LB cells began to elongate LB-Mac-	PMID:2176633
mbrA15			Growth Phenotype	temperature sensitive, forms two distinct populations, one more dense and one less dense than wild type	PMID:2176633
mbrA4			Resistant to	camphor resistance	PMID:2176633 PMID:1925019		Table 2 and table 1
mrbA15			Resistant to	Resistant to camphor	PMID:2176633 PMID:1925019		table 2 and table 1
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW5604	Plasmid clone	Shigen	PMID:16769691 Status:cloned as old JW Primer 1:GCCCGTCTAAACCCCGGCCAACA Primer 2:CCTTTCCCTCGTTTTGCCGCCAT
2A8	Kohara Phage	Genobase	PMID:3038334
2E6	Kohara Phage	Genobase	PMID:3038334
ilvD500::Tn10	Linked marker	CAG18431 = CGSC7462^[5]	est. P1 cotransduction: 83% ^[6] Synonyms:ilv500::Tn10
metEo-3079::Tn10	Linked marker	CAG18491 = CGSC7464^[5]	est. P1 cotransduction: 10% ^[6] Synonyms:metE3079::Tn10 nnnThe Tn10 in CAG13491 is inserted in the metE regulatory region, but causes a Met- phenotype.
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10837	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10837	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000828	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:948292	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0830	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0012344	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	Rep
Synonyms	DNA helicase and single-stranded DNA-dependent ATPase^[1], B3778^[2]^[1], MmrA^[2]^[1], MbrA^[2]^[1], DasC^[2]^[1], Rep^[2]^[1] , dasC, ECK3770, JW5604, mbrA, mmrA?, b3778
Product description	rep^[2]^[3]; Component of rep helicase, a single-stranded DNA dependent ATPase^[3] ATP-dependent DNA helicase Rep, involved in DNA replication; ssDNA-dependent ATPase^[4]
EC number (for enzymes)	3.6.1.-^[1]
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0000166	nucleotide binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0547	F	Seeded from EcoCyc (v14.0)	complete
	GO:0003677	DNA binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR000212	F	Seeded from EcoCyc (v14.0)	complete
	GO:0003677	DNA binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0238	F	Seeded from EcoCyc (v14.0)	complete
	GO:0004003	ATP-dependent DNA helicase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR000212	F	Seeded from EcoCyc (v14.0)	complete
	GO:0004003	ATP-dependent DNA helicase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR005752	F	Seeded from EcoCyc (v14.0)	complete
	GO:0004386	helicase activity	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0347	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005524	ATP binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR000212	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005524	ATP binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR014016	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005524	ATP binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR014017	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005524	ATP binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0067	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005737	cytoplasm	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR005752	C	Seeded from EcoCyc (v14.0)	complete
	GO:0006260	DNA replication	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0235	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006268	DNA unwinding involved in replication	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR005752	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006281	DNA repair	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR000212	P	Seeded from EcoCyc (v14.0)	complete
	GO:0016787	hydrolase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR014016	F	Seeded from EcoCyc (v14.0)	complete
	GO:0016787	hydrolase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR014017	F	Seeded from EcoCyc (v14.0)	complete
	GO:0016787	hydrolase activity	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0378	F	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	Subunits of rep helicase, a single-stranded DNA dependent ATPase	could be indirect
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MRLNPGQQQA VEFVTGPCLV LAGAGSGKTR VITNKIAHLI RGCGYQARHI AAVTFTNKAA REMKERVGQT LGRKEARGLM ISTFHTLGLD IIKREYAALG MKANFSLFDD TDQLALLKEL TEGLIEDDKV LLQQLISTIS NWKNDLKTPS QAAASAIGER DRIFAHCYGL YDAHLKACNV LDFDDLILLP TLLLQRNEEV RKRWQNKIRY LLVDEYQDTN TSQYELVKLL VGSRARFTVV GDDDQSIYSW RGARPQNLVL LSQDFPALKV IKLEQNYRSS GRILKAANIL IANNPHVFEK RLFSELGYGA ELKVLSANNE EHEAERVTGE LIAHHFVNKT QYKDYAILYR GNHQSRVFEK FLMQNRIPYK ISGGTSFFSR PEIKDLLAYL RVLTNPDDDS AFLRIVNTPK REIGPATLKK LGEWAMTRNK SMFTASFDMG LSQTLSGRGY EALTRFTHWL AEIQRLAERE PIAAVRDLIH GMDYESWLYE TSPSPKAAEM RMKNVNQLFS WMTEMLEGSE LDEPMTLTQV VTRFTLRDMM ERGESEEELD QVQLMTLHAS KGLEFPYVYM VGMEEGFLPH QSSIDEDNID EERRLAYVGI TRAQKELTFT LCKERRQYGE LVRPEPSRFL LELPQDDLIW EQERKVVSAE ERMQKGQSHL ANLKAMMAAK RGK
Length	673
Mol. Wt	77.026 kDa
pI	7.2 (calculated)
Extinction coefficient	76,780 - 77,405 (calc based on 22 Y, 8 W, and 5 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	3..484	PF00580 UvrD/REP helicase		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=rep taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:49176407	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0012344	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P09980	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG10837	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10837	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:948292	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000828	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:948292	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0830	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	Ecoli K-12	3.292+/-0.069	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		Single Molecule Fluorescence	PMID:20671182
mRNA	Ecoli K-12	0.025729837	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		by RNA_Seq	PMID:20671182
Protein	E. coli K-12 MG1655	453	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	102	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	224	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
rep
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:3958680..3958720 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b3778 (EcoliWiki Page)	NCBI GEO profiles for rep
microarray	GenExpDB:b3778 (EcoliWiki Page)	Summary of data for rep from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to rep Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10837	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0830	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b3778
EcoGene	EcoGene:EG10837	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000828	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0012344	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Shigella flexneri	REP	From SHIGELLACYC
E. coli O157	REP	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Superfamily (EcoliWiki Page)	SUPERFAMILY:52540
Pfam (EcoliWiki Page)	PF00580 UvrD/REP helicase
EcoCyc	EcoCyc:EG10837	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10837	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000828	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0830	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0012344	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 ^1.12 ^1.13 ^1.14 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 ^2.11 ^2.12 ^2.13 ^2.14 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 ^3.3 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

rep:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to rep Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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