MDS42

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Alternate Names

Sources

Scarab Genomics

Chromosomal Genotype

MG1655 fhuACDB(del) endA(del) + deletion of 699 additional genes, including all IS elements and cryptic prophages[1]

Plasmids

Phage

Phenotypes

Construction

Most of the deletions in MDS42 were constructed by lambda Red-mediated recombination of linear fragments into the genome, followed by removal of exogenous sequences by double strand break (DSB)-stimulated recombination.[1] The individual deletions are described in the Supporting Online Material.

Notes

Compared to MG1655, MDS42 has 663,316 bp deleted (14.3% of the original genome).[1] Deletion of fhuACDB confers resistance to phage T1. The endA gene was deleted to improve the integrity of plasmid DNA isolated from this strain.

References

See Help:References for how to manage references in EcoliWiki. [1]

  1. ↑ 1.0 1.1 1.2 1.3 Pósfai, G et al. (2006) Emergent properties of reduced-genome Escherichia coli. Science 312 1044-6 PubMed
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