recB:On One Page

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Quickview | Gene | Product(s) | Expression| Evolution | References |

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Standard Name

recB

Gene Synonym(s)

ECK2816, b2820, JW2788, rorA, ior[1], ior

Product Desc.

RecB; DNA helicase, ATP-dependent dsDNA/ssDNA exonuclease V subunit, ssDNA endonuclease[2][3];

Component of recBCD[2][3]

RecBCD Exonuclease V subunit, recombination and repair; chi-activated RecBCD recombinase subunit; RecBCD 3'-5' slow helicase subunit; contains nuclease active site and ATP-binding site; RecB alone has 3'-5' helicase activity; binds RecC[4]

Product Synonyms(s)

exonuclease V (RecBCD complex), beta subunit[1], B2820[2][1], Ior[2][1], RorA[2][1], RecB[2][1] , ECK2816, ior, JW2788, rorA, b2820

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): recD[2]

Regulation/Activity
Quick Links

porteco.png EcoCyc.gif regulondb.jpg

dnadisplay.png proteindisplay.png   pubmed.jpg   textpresso.jpg  

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Notes

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RecBCD is a bipolar helicase.[4]



Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

recB

Mnemonic

Recombination

Synonyms

ECK2816, b2820, JW2788, rorA, ior[1], ior

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

63.59 minutes 

MG1655: 2954025..2950483
<gbrowseImage> name=NC_000913:2950483..2954025 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 2850622..2847080
<gbrowseImage> name=NC_012967:2847080..2850622 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 2837631..2841173
<gbrowseImage> name=NC_012759:2837631..2841173 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 2954659..2951117
<gbrowseImage> name=NC_007779:2951117..2954659 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 3047895..3044353
<gbrowseImage> name=NC_010473:3044353..3047895 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

2950486

Edman degradation

PMID:1618858


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

recB(del) (Keio:JW2788)

deletion

deletion

PMID:16738554

Shigen
CGSC10182[5]

recB::Tn5KAN-I-SceI (FB20945)

Insertion at nt 2423 in Minus orientation

PMID:15262929

E. coli Genome Project:FB20945

contains pKD46

recB::Tn5KAN-I-SceI (FB20946)

Insertion at nt 2423 in Minus orientation

PMID:15262929

E. coli Genome Project:FB20946

does not contain pKD46

recB21

CGSC:4677

recB270(ts)

temperature sensitive

CGSC:8994

recB1067(D1067A)

PMID:9790841

CGSC:71962

recB1080(D1080A)

PMID:11532156

CGSC:72006

recB1082(K1082Q)

PMID:9790841

CGSC:72009

recB60

CGSC:84445

recB745(del)::kan

PMID:16738554

CGSC:102622

recB in MDS42

deletion

Sensitivity to

increases sensitivity to bicyclomycin

PMID:21183718

fig. 2

recB

deletion

Sensitivity to

decreases sensitivity to Bicyclomycin

PMID:21357484

fig 2

nrdA101 recB(del)

recB function is deactivated

Rate of DNA replication

When exposed to extreme temperatures the rate of transcription is slightly under restrictive conditions

PMID:21441507

See Figure 2

recB268::Tn10

Tn10 was inserted into the recB gene at the allele 268.

Growth Phenotype

the rate of growth was significantly decreased.

PMID:9973363

See figure 1. The strain background is Hfr3000; called '3000HfrH' in the paper.

SMR6039 recB(del)::Kan

Deletion

SOS response

Decrease in spontaneous SOS-induction defective

PMID:23224554

Parent Strain: SMR6039 Experimental Strain: SMR16024

The mutation caused a defect in Spontaneous SOS-Induction. See table S7 for a summary of experimental results.

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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW2788

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCAGTGATGTCGCCGAGACACT

Primer 2:CCgGCCTCCTCCAGGGTCATACC

10B6

Kohara Phage

Genobase

PMID:3038334

3G11

Kohara Phage

Genobase

PMID:3038334

fucP3072::Tn10

Linked marker

CAG12079 = CGSC7427[5]

est. P1 cotransduction: 52% [6]
Synonyms:fuc-3072::Tn10

recD1901::Tn10

Linked marker

CAG12135 = CGSC7429[5]

est. P1 cotransduction: 99% [6]

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10824

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10824

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000815

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:947286

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0817

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0009249

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

RecB

Synonyms

exonuclease V (RecBCD complex), beta subunit[1], B2820[2][1], Ior[2][1], RorA[2][1], RecB[2][1] , ECK2816, ior, JW2788, rorA, b2820

Product description

RecB; DNA helicase, ATP-dependent dsDNA/ssDNA exonuclease V subunit, ssDNA endonuclease[2][3];

Component of recBCD[2][3]

RecBCD Exonuclease V subunit, recombination and repair; chi-activated RecBCD recombinase subunit; RecBCD 3'-5' slow helicase subunit; contains nuclease active site and ATP-binding site; RecB alone has 3'-5' helicase activity; binds RecC[4]

EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status

GO:0005737

cytoplasm

C

Seeded from Riley et al 2006 [1].

Missing: evidence, reference

GO:0000166

nucleotide binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0547

F

Seeded from EcoCyc (v14.0)

complete

GO:0003677

DNA binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000212

F

Seeded from EcoCyc (v14.0)

complete

GO:0003677

DNA binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR011604

F

Seeded from EcoCyc (v14.0)

complete

GO:0004003

ATP-dependent DNA helicase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000212

F

Seeded from EcoCyc (v14.0)

complete

GO:0004386

helicase activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0347

F

Seeded from EcoCyc (v14.0)

complete

GO:0004518

nuclease activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR011604

F

Seeded from EcoCyc (v14.0)

complete

GO:0004518

nuclease activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0540

F

Seeded from EcoCyc (v14.0)

complete

GO:0004519

endonuclease activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0255

F

Seeded from EcoCyc (v14.0)

complete

GO:0004527

exonuclease activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0269

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000212

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004586

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR014016

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR014017

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

PMID:3298248

IDA: Inferred from Direct Assay

F

complete

GO:0005524

ATP binding

PMID:3298248

IDA: Inferred from Direct Assay

F

complete

GO:0005524

ATP binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0067

F

Seeded from EcoCyc (v14.0)

complete

GO:0006281

DNA repair

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000212

P

Seeded from EcoCyc (v14.0)

complete

GO:0006281

DNA repair

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004586

P

Seeded from EcoCyc (v14.0)

complete

GO:0006281

DNA repair

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0234

P

Seeded from EcoCyc (v14.0)

complete

GO:0006974

response to DNA damage stimulus

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0227

P

Seeded from EcoCyc (v14.0)

complete

GO:0008854

exodeoxyribonuclease V activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004586

F

Seeded from EcoCyc (v14.0)

complete

GO:0008854

exodeoxyribonuclease V activity

GOA:spec
GO_REF:0000003

IEA: Inferred from Electronic Annotation

EC:3.1.11.5

F

Seeded from EcoCyc (v14.0)

complete

GO:0016787

hydrolase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR014016

F

Seeded from EcoCyc (v14.0)

complete

GO:0016787

hydrolase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR014017

F

Seeded from EcoCyc (v14.0)

complete

GO:0016787

hydrolase activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0378

F

Seeded from EcoCyc (v14.0)

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

Subunits of recBCD

could be indirect

Protein

aceE

PMID:15690043

Experiment(s):EBI-882076

Protein

aceF

PMID:15690043

Experiment(s):EBI-882076

Protein

lpdA

PMID:15690043

Experiment(s):EBI-882076

Protein

recD

PMID:15690043

Experiment(s):EBI-882076, EBI-882133

Protein

rpsB

PMID:15690043

Experiment(s):EBI-882076

Protein

tufA

PMID:15690043

Experiment(s):EBI-882076

Protein

recC

PMID:15690043

Experiment(s):EBI-882076

Protein

rplE

PMID:16606699

Experiment(s):EBI-1144059

Protein

groL

PMID:16606699

Experiment(s):EBI-1144059

Protein

carB

PMID:16606699

Experiment(s):EBI-1144059

Protein

rplF

PMID:16606699

Experiment(s):EBI-1144059

Protein

prs

PMID:16606699

Experiment(s):EBI-1144059

Protein

rplB

PMID:16606699

Experiment(s):EBI-1144059

Protein

recD

PMID:19402753

MALDI(Z-score):28.692624

Protein

recC

PMID:19402753

MALDI(Z-score):19.012184

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MSDVAETLDP LRLPLQGERL IEASAGTGKT FTIAALYLRL LLGLGGSAAF PRPLTVEELL
VVTFTEAATA ELRGRIRSNI HELRIACLRE TTDNPLYERL LEEIDDKAQA AQWLLLAERQ
MDEAAVFTIH GFCQRMLNLN AFESGMLFEQ QLIEDESLLR YQACADFWRR HCYPLPREIA
QVVFETWKGP QALLRDINRY LQGEAPVIKA PPPDDETLAS RHAQIVARID TVKQQWRDAV
GELDALIESS GIDRRKFNRS NQAKWIDKIS AWAEEETNSY QLPESLEKFS QRFLEDRTKA
GGETPRHPLF EAIDQLLAEP LSIRDLVITR ALAEIRETVA REKRRRGELG FDDMLSRLDS
ALRSESGEVL AAAIRTRFPV AMIDEFQDTD PQQYRIFRRI WHHQPETALL LIGDPKQAIY
AFRGADIFTY MKARSEVHAH YTLDTNWRSA PGMVNSVNKL FSQTDDAFMF REIPFIPVKS
AGKNQALRFV FKGETQPAMK MWLMEGESCG VGDYQSTMAQ VCAAQIRDWL QAGQRGEALL
MNGDDARPVR ASDISVLVRS RQEAAQVRDA LTLLEIPSVY LSNRDSVFET LEAQEMLWLL
QAVMTPEREN TLRSALATSM MGLNALDIET LNNDEHAWDV VVEEFDGYRQ IWRKRGVMPM
LRALMSARNI AENLLATAGG ERRLTDILHI SELLQEAGTQ LESEHALVRW LSQHILEPDS
NASSQQMRLE SDKHLVQIVT IHKSKGLEYP LVWLPFITNF RVQEQAFYHD RHSFEAVLDL
NAAPESVDLA EAERLAEDLR LLYVALTRSV WHCSLGVAPL VRRRGDKKGD TDVHQSALGR
LLQKGEPQDA AGLRTCIEAL CDDDIAWQTA QTGDNQPWQV NDVSTAELNA KTLQRLPGDN
WRVTSYSGLQ QRGHGIAQDL MPRLDVDAAG VASVVEEPTL TPHQFPRGAS PGTFLHSLFE
DLDFTQPVDP NWVREKLELG GFESQWEPVL TEWITAVLQA PLNETGVSLS QLSARNKQVE
MEFYLPISEP LIASQLDTLI RQFDPLSAGC PPLEFMQVRG MLKGFIDLVF RHEGRYYLLD
YKSNWLGEDS SAYTQQAMAA AMQAHRYDLQ YQLYTLALHR YLRHRIADYD YEHHFGGVIY
LFLRGVDKEH PQQGIYTTRP NAGLIALMDE MFAGMTLEEA
Length

1,180

Mol. Wt

133.961 kDa

pI

5.1 (calculated)

Extinction coefficient

171,200 - 172,450 (calc based on 30 Y, 23 W, and 10 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

4..708

PF00580 UvrD/REP helicase

PMID:19920124

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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=recB taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16130724

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:947286

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0009249

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P08394

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10824

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10824

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:947286

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000815

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0817

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

Protein

Ecoli K-12

0.607+/-0.152

Molecules/cell

  • Medium:
  • Temperature (°C):
  • Doubling time (min): Growth Rate(min): *
  • Growth phase: other: *

Single Molecule Fluorescence

PMID:20671182

mRNA

Ecoli K-12

0.023433089

Molecules/cell

  • Medium:
  • Temperature (°C):
  • Doubling time (min): Growth Rate(min): *
  • Growth phase: other: *

by RNA_Seq

PMID:20671182

Protein

E. coli K-12 MG1655

93

molecules/cell/generation

  • Medium: MOPS Complete
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

36

molecules/cell/generation

  • Medium: MOPS Minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

33

molecules/cell/generation

  • Medium: MOPS Complete without Methionine
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

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Notes

Transcription and Transcriptional Regulation

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<protect>

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

recD

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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:2954005..2954045 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GEO Profiles:b2820 (EcoliWiki Page)

NCBI GEO profiles for recB

microarray

GenExpDB:b2820 (EcoliWiki Page)

Summary of data for recB from multiple microarray studies

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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes
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Notes

Accessions Related to recB Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10824

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0817

Escherichia coli str. K-12 substr. MG1655

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b2820

EcoGene

EcoGene:EG10824

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000815

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0009249

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism Homologs (Statistics) Comments

Shigella flexneri

RECB

From SHIGELLACYC

E. coli O157

RECB

From ECOO157CYC

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Do-It-Yourself Web Tools

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Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

Superfamily (EcoliWiki Page)

SUPERFAMILY:52540

Superfamily (EcoliWiki Page)

SUPERFAMILY:52980

Panther (EcoliWiki Page)

PTHR11070:SF0

Pfam (EcoliWiki Page)

PF00580 UvrD/REP helicase

EcoCyc

EcoCyc:EG10824

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10824

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000815

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0817

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0009249

Escherichia coli str. K-12 substr. MG1655

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  1. ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 3.2 3.3 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ 4.0 4.1 4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
  6. ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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