recB:Gene Product(s)

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Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

RecB

Synonyms

exonuclease V (RecBCD complex), beta subunit[1], B2820[2][1], Ior[2][1], RorA[2][1], RecB[2][1] , ECK2816, ior, JW2788, rorA, b2820

Product description

RecB; DNA helicase, ATP-dependent dsDNA/ssDNA exonuclease V subunit, ssDNA endonuclease[2][3];

Component of recBCD[2][3]

RecBCD Exonuclease V subunit, recombination and repair; chi-activated RecBCD recombinase subunit; RecBCD 3'-5' slow helicase subunit; contains nuclease active site and ATP-binding site; RecB alone has 3'-5' helicase activity; binds RecC[4]

EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0005737

cytoplasm

C

Seeded from Riley et al 2006 [1].

Missing: evidence, reference

GO:0000166

nucleotide binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0547

F

Seeded from EcoCyc (v14.0)

complete

GO:0003677

DNA binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000212

F

Seeded from EcoCyc (v14.0)

complete

GO:0003677

DNA binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR011604

F

Seeded from EcoCyc (v14.0)

complete

GO:0004003

ATP-dependent DNA helicase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000212

F

Seeded from EcoCyc (v14.0)

complete

GO:0004386

helicase activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0347

F

Seeded from EcoCyc (v14.0)

complete

GO:0004518

nuclease activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR011604

F

Seeded from EcoCyc (v14.0)

complete

GO:0004518

nuclease activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0540

F

Seeded from EcoCyc (v14.0)

complete

GO:0004519

endonuclease activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0255

F

Seeded from EcoCyc (v14.0)

complete

GO:0004527

exonuclease activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0269

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000212

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004586

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR014016

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR014017

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

PMID:3298248[5]

IDA: Inferred from Direct Assay

F

complete

GO:0005524

ATP binding

PMID:3298248[5]

IDA: Inferred from Direct Assay

F

complete

GO:0005524

ATP binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0067

F

Seeded from EcoCyc (v14.0)

complete

GO:0006281

DNA repair

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000212

P

Seeded from EcoCyc (v14.0)

complete

GO:0006281

DNA repair

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004586

P

Seeded from EcoCyc (v14.0)

complete

GO:0006281

DNA repair

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0234

P

Seeded from EcoCyc (v14.0)

complete

GO:0006974

response to DNA damage stimulus

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0227

P

Seeded from EcoCyc (v14.0)

complete

GO:0008854

exodeoxyribonuclease V activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004586

F

Seeded from EcoCyc (v14.0)

complete

GO:0008854

exodeoxyribonuclease V activity

GOA:spec
GO_REF:0000003

IEA: Inferred from Electronic Annotation

EC:3.1.11.5

F

Seeded from EcoCyc (v14.0)

complete

GO:0016787

hydrolase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR014016

F

Seeded from EcoCyc (v14.0)

complete

GO:0016787

hydrolase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR014017

F

Seeded from EcoCyc (v14.0)

complete

GO:0016787

hydrolase activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0378

F

Seeded from EcoCyc (v14.0)

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

Subunits of recBCD

could be indirect

Protein

aceE

PMID:15690043[6]

Experiment(s):EBI-882076

Protein

aceF

PMID:15690043[6]

Experiment(s):EBI-882076

Protein

lpdA

PMID:15690043[6]

Experiment(s):EBI-882076

Protein

recD

PMID:15690043[6]

Experiment(s):EBI-882076, EBI-882133

Protein

rpsB

PMID:15690043[6]

Experiment(s):EBI-882076

Protein

tufA

PMID:15690043[6]

Experiment(s):EBI-882076

Protein

recC

PMID:15690043[6]

Experiment(s):EBI-882076

Protein

rplE

PMID:16606699[7]

Experiment(s):EBI-1144059

Protein

groL

PMID:16606699[7]

Experiment(s):EBI-1144059

Protein

carB

PMID:16606699[7]

Experiment(s):EBI-1144059

Protein

rplF

PMID:16606699[7]

Experiment(s):EBI-1144059

Protein

prs

PMID:16606699[7]

Experiment(s):EBI-1144059

Protein

rplB

PMID:16606699[7]

Experiment(s):EBI-1144059

Protein

recD

PMID:19402753[8]

MALDI(Z-score):28.692624

Protein

recC

PMID:19402753[8]

MALDI(Z-score):19.012184

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MSDVAETLDP LRLPLQGERL IEASAGTGKT FTIAALYLRL LLGLGGSAAF PRPLTVEELL
VVTFTEAATA ELRGRIRSNI HELRIACLRE TTDNPLYERL LEEIDDKAQA AQWLLLAERQ
MDEAAVFTIH GFCQRMLNLN AFESGMLFEQ QLIEDESLLR YQACADFWRR HCYPLPREIA
QVVFETWKGP QALLRDINRY LQGEAPVIKA PPPDDETLAS RHAQIVARID TVKQQWRDAV
GELDALIESS GIDRRKFNRS NQAKWIDKIS AWAEEETNSY QLPESLEKFS QRFLEDRTKA
GGETPRHPLF EAIDQLLAEP LSIRDLVITR ALAEIRETVA REKRRRGELG FDDMLSRLDS
ALRSESGEVL AAAIRTRFPV AMIDEFQDTD PQQYRIFRRI WHHQPETALL LIGDPKQAIY
AFRGADIFTY MKARSEVHAH YTLDTNWRSA PGMVNSVNKL FSQTDDAFMF REIPFIPVKS
AGKNQALRFV FKGETQPAMK MWLMEGESCG VGDYQSTMAQ VCAAQIRDWL QAGQRGEALL
MNGDDARPVR ASDISVLVRS RQEAAQVRDA LTLLEIPSVY LSNRDSVFET LEAQEMLWLL
QAVMTPEREN TLRSALATSM MGLNALDIET LNNDEHAWDV VVEEFDGYRQ IWRKRGVMPM
LRALMSARNI AENLLATAGG ERRLTDILHI SELLQEAGTQ LESEHALVRW LSQHILEPDS
NASSQQMRLE SDKHLVQIVT IHKSKGLEYP LVWLPFITNF RVQEQAFYHD RHSFEAVLDL
NAAPESVDLA EAERLAEDLR LLYVALTRSV WHCSLGVAPL VRRRGDKKGD TDVHQSALGR
LLQKGEPQDA AGLRTCIEAL CDDDIAWQTA QTGDNQPWQV NDVSTAELNA KTLQRLPGDN
WRVTSYSGLQ QRGHGIAQDL MPRLDVDAAG VASVVEEPTL TPHQFPRGAS PGTFLHSLFE
DLDFTQPVDP NWVREKLELG GFESQWEPVL TEWITAVLQA PLNETGVSLS QLSARNKQVE
MEFYLPISEP LIASQLDTLI RQFDPLSAGC PPLEFMQVRG MLKGFIDLVF RHEGRYYLLD
YKSNWLGEDS SAYTQQAMAA AMQAHRYDLQ YQLYTLALHR YLRHRIADYD YEHHFGGVIY
LFLRGVDKEH PQQGIYTTRP NAGLIALMDE MFAGMTLEEA
Length

1,180

Mol. Wt

133.961 kDa

pI

5.1 (calculated)

Extinction coefficient

171,200 - 172,450 (calc based on 30 Y, 23 W, and 10 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

4..708

PF00580 UvrD/REP helicase

PMID:19920124[9]

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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=recB taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16130724

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:947286

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0009249

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P08394

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10824

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10824

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:947286

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000815

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0817

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 2.4 2.5 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 Julin, DA & Lehman, IR (1987) Photoaffinity labeling of the recBCD enzyme of Escherichia coli with 8-azidoadenosine 5'-triphosphate. J. Biol. Chem. 262 9044-51 PubMed
  6. ↑ 6.0 6.1 6.2 6.3 6.4 6.5 6.6 Butland, G et al. (2005) Interaction network containing conserved and essential protein complexes in Escherichia coli. Nature 433 531-7 PubMed
  7. ↑ 7.0 7.1 7.2 7.3 7.4 7.5 Arifuzzaman, M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res. 16 686-91 PubMed
  8. ↑ 8.0 8.1 Hu, P et al. (2009) Global functional atlas of Escherichia coli encompassing previously uncharacterized proteins. PLoS Biol. 7 e96 PubMed
  9. ↑ Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed

Categories

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