proA:On One Page
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Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
Quickview | | Gene | | Product(s) | | Expression| | Evolution | | References | |
Quickview
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Standard Name |
proA |
---|---|
Gene Synonym(s) |
ECK0244, b0243, JW0233, pro1, pro(1)[1], pro |
Product Desc. |
Component of glutamate-5-semialdehyde dehydrogenase[2][3]; γ-glutamyl kinase-GP-reductase multienzyme complex[2][3] gamma-Glutamyl phosphate reductase, proline biosynthesis[4] |
Product Synonyms(s) |
gamma-glutamylphosphate reductase[1], B0243[2][1], ProA[2][1] , ECK0244, JW0233, pro, b0243 |
Function from GO |
<GO_nr /> |
Knock-Out Phenotype | |
Regulation/Expression | |
Regulation/Activity | |
Quick Links | |
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</protect> See Help:Quickview for help with entering information in the Quickview table. <protect></protect>
Notes
Gene
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Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
Standard name |
proA |
---|---|
Mnemonic |
Proline |
Synonyms |
ECK0244, b0243, JW0233, pro1, pro(1)[1], pro |
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Notes
Location(s) and DNA Sequence
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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
---|---|---|---|---|
MG1655 |
5.62 minutes |
MG1655: 260727..261980 |
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NC_012967: 265995..267248 |
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NC_012759: 260726..261979 |
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W3110 |
|
W3110: 260727..261980 |
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DH10B: 234831..236084 |
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Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
---|---|---|---|---|---|
Coding Start (SO:0000323) |
260727 |
Edman degradation |
PMID:6089111 |
| |
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Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
---|---|---|---|---|---|---|---|
ΔproA (Keio:JW0233) |
deletion |
deletion |
Auxotrophies |
Requires proline for growth |
PMID:16738554 |
||
proA::Tn5KAN-2 (FB20067) |
Insertion at nt 192 in Plus orientation |
Auxotrophies |
Requires proline for growth |
PMID:15262929 |
does not contain pKD46 | ||
proA81::Tn10 |
Insertion at 261,286 bp in MG1655 (NC_000913) |
Auxotrophies |
Requires proline for growth |
adapted from Nichols et al.[6] |
Synonyms: | ||
proA761(del)::kan |
insertion |
insertion |
Auxotrophies |
Requires proline for growth |
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proA23 |
Auxotrophies |
Requires proline for growth |
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proA44 |
Auxotrophies |
Requires proline for growth |
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proA81::Tn10 |
Auxotrophies |
Requires proline for growth |
PMID:9829956 |
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proA35 |
Auxotrophies |
Requires proline for growth |
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proA30 |
Auxotrophies |
Requires proline for growth |
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proA181 |
Auxotrophies |
Requires proline for growth |
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proA180 |
Auxotrophies |
Requires proline for growth |
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proA |
Auxotrophies |
Requires proline for growth |
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proA3096::Tn10kan |
Auxotrophies |
Requires proline for growth |
PMID:2540407 PMID:9829956 |
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ΔproA761::kan |
deletion |
deletion |
Auxotrophies |
Requires proline for growth |
PMID:16738554 |
||
proA |
Resistant to |
Resistant to Nitro(4)pyridine N-oxide |
PMID:4598010 |
||||
proA |
Resistant to |
Nitro-4-pyridine N-oxide Resistance |
PMID:385587 |
Strain: W3630 |
table 1 | ||
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Notes
Genetic Interactions
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Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
---|---|---|---|---|---|---|
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Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
Resource | Resource Type | Source | Notes/Reference |
---|---|---|---|
JW0233 |
Plasmid clone |
PMID:16769691 Status:Clone OK Primer 1:GCCCTGGAACAAATGGGCATTGC Primer 2:CCCGCACGAATGGTGTAATCACC | |
Kohara Phage |
PMID:3038334 | ||
Kohara Phage |
PMID:3038334 | ||
Linked marker |
est. P1 cotransduction: 33% [6] | ||
Linked marker |
est. P1 cotransduction: % [6] | ||
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
Database | Accession | Notes |
---|---|---|
EcoCyc:EG10767 |
Escherichia coli str. K-12 substr. MG1655 | |
EcoGene:EG10767 |
Escherichia coli str. K-12 substr. MG1655 | |
RegulonDB:ECK120000758 |
Escherichia coli str. K-12 substr. MG1655 | |
Escherichia coli str. K-12 substr. MG1655 | ||
EchoBASE:EB0760 |
Escherichia coli str. K-12 substr. MG1655 | |
ASAP:ABE-0000832 |
Escherichia coli str. K-12 substr. MG1655 | |
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Notes
Links
Name | URL | Comments |
---|---|---|
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Product(s)
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Nomenclature
See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.
Standard name |
ProA |
---|---|
Synonyms |
gamma-glutamylphosphate reductase[1], B0243[2][1], ProA[2][1] , ECK0244, JW0233, pro, b0243 |
Product description |
Component of glutamate-5-semialdehyde dehydrogenase[2][3]; γ-glutamyl kinase-GP-reductase multienzyme complex[2][3] gamma-Glutamyl phosphate reductase, proline biosynthesis[4] |
EC number (for enzymes) | |
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Notes
Function
<protect>
Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.
Qualifier | GO ID | GO term name | Reference | Evidence Code | with/from | Aspect | Notes | Status |
---|---|---|---|---|---|---|---|---|
GO:0004350 |
glutamate-5-semialdehyde dehydrogenase activity |
GOA:hamap |
IEA: Inferred from Electronic Annotation |
HAMAP:MF_00412 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0004350 |
glutamate-5-semialdehyde dehydrogenase activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR000965 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0004350 |
glutamate-5-semialdehyde dehydrogenase activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR012134 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0004350 |
glutamate-5-semialdehyde dehydrogenase activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR020593 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0004350 |
glutamate-5-semialdehyde dehydrogenase activity |
GOA:spec |
IEA: Inferred from Electronic Annotation |
EC:1.2.1.41 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0004350 |
glutamate-5-semialdehyde dehydrogenase activity |
PMID:6337636 |
IDA: Inferred from Direct Assay |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
GO:0004350 |
glutamate-5-semialdehyde dehydrogenase activity |
PMID:7034716 |
IDA: Inferred from Direct Assay |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
GO:0004350 |
glutamate-5-semialdehyde dehydrogenase activity |
PMID:7035170 |
IDA: Inferred from Direct Assay |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
GO:0005737 |
cytoplasm |
GOA:hamap |
IEA: Inferred from Electronic Annotation |
HAMAP:MF_00412 |
C |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0005737 |
cytoplasm |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0963 |
C |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0005737 |
cytoplasm |
GO_REF:0000023 |
IEA: Inferred from Electronic Annotation |
SP_SL:SL-0086 |
C |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0006561 |
proline biosynthetic process |
GOA:hamap |
IEA: Inferred from Electronic Annotation |
HAMAP:MF_00412 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0006561 |
proline biosynthetic process |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR000965 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0006561 |
proline biosynthetic process |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR012134 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0006561 |
proline biosynthetic process |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR020593 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0006561 |
proline biosynthetic process |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0641 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0006561 |
proline biosynthetic process |
PMID:6089111 |
IGI: Inferred from Genetic Interaction |
P |
Seeded from EcoCyc (v14.0) |
Missing: with/from | ||
GO:0050661 |
NADP or NADPH binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR012134 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0050661 |
NADP or NADPH binding |
PMID:7034716 |
IDA: Inferred from Direct Assay |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
GO:0055114 |
oxidation reduction |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR012134 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0055114 |
oxidation reduction |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR015590 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0055114 |
oxidation reduction |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR016161 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0055114 |
oxidation reduction |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR016162 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0055114 |
oxidation reduction |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0560 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.
Partner Type | Partner | Notes | References | Evidence |
---|---|---|---|---|
Protein |
Subunits of glutamate-5-semialdehyde dehydrogenase |
could be indirect |
||
Protein |
aceE |
PMID:15690043 |
Experiment(s):EBI-888834 | |
Protein |
dnaN |
PMID:15690043 |
Experiment(s):EBI-888834 | |
Protein |
rapA |
PMID:16606699 |
Experiment(s):EBI-1136008 | |
Protein |
yhhH |
PMID:16606699 |
Experiment(s):EBI-1136008 | |
Protein |
ptrA |
PMID:16606699 |
Experiment(s):EBI-1136008 | |
Protein |
iscS |
PMID:16606699 |
Experiment(s):EBI-1136008 | |
Protein |
yghO |
PMID:15690043 |
Experiment(s):EBI-893980 | |
Protein |
cysA |
PMID:15690043 |
Experiment(s):EBI-893980 | |
Protein |
lpxD |
PMID:15690043 |
Experiment(s):EBI-893980 | |
Protein |
proS |
PMID:15690043 |
Experiment(s):EBI-893980 | |
Protein |
sfsA |
PMID:15690043 |
Experiment(s):EBI-893980 | |
Protein |
ugpB |
PMID:15690043 |
Experiment(s):EBI-893980 | |
Protein |
yehT |
PMID:15690043 |
Experiment(s):EBI-893980 | |
Protein |
proS |
PMID:19402753 |
LCMS(ID Probability):99.0 | |
Protein |
cysA |
PMID:19402753 |
LCMS(ID Probability):99.0 | |
Protein |
yehT |
PMID:19402753 |
LCMS(ID Probability):99.0 | |
Protein |
sfsA |
PMID:19402753 |
LCMS(ID Probability):99.0 | |
Protein |
Subunits of γ-glutamyl kinase-GP-reductase multienzyme complex |
could be indirect |
| |
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</protect>
Notes
Localization
See Help:Product_localization for how to add or edit information in this section of EcoliWiki.
Compartment | Description | Evidence | Reference/Source | Notes |
---|---|---|---|---|
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Notes
Structure and Physical Properties
<protect>
Physical Properties
See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.
Name | |
---|---|
Sequence |
MLEQMGIAAK QASYKLAQLS SREKNRVLEK IADELEAQSE IILNANAQDV ADARANGLSE AMLDRLALTP ARLKGIADDV RQVCNLADPV GQVIDGGVLD SGLRLERRRV PLGVIGVIYE ARPNVTVDVA SLCLKTGNAV ILRGGKETCR TNAATVAVIQ DALKSCGLPA GAVQAIDNPD RALVSEMLRM DKYIDMLIPR GGAGLHKLCR EQSTIPVITG GIGVCHIYVD ESVEIAEALK VIVNAKTQRP STCNTVETLL VNKNIADSFL PALSKQMAES GVTLHADAAA LAQLQAGPAK VVAVKAEEYD DEFLSLDLNV KIVSDLDDAI AHIREHGTQH SDAILTRDMR NAQRFVNEVD SSAVYVNAST RFTDGGQFGL GAEVAVSTQK LHARGPMGLE ALTTYKWIGI GDYTIRA |
Length |
417 |
Mol. Wt |
44.629 kDa |
pI |
5.4 (calculated) |
Extinction coefficient |
17,420 - 18,295 (calc based on 8 Y, 1 W, and 7 C residues) |
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Domains/Motifs/Modification Sites
See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.
|
<motif_map/> |
Structure
| </protect>
Structure figures<protect> | ||||||
Notes
Gene Product Resources
See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.
Resource type | Source | Notes/Reference |
---|---|---|
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
Database | Accession | Notes |
---|---|---|
Escherichia coli str. K-12 substr. MG1655 | ||
Escherichia coli str. K-12 substr. MG1655 | ||
ASAP:ABE-0000832 |
Escherichia coli str. K-12 substr. MG1655 | |
Escherichia coli str. K-12 substr. MG1655 | ||
EcoCyc:EG10767 |
Escherichia coli str. K-12 substr. MG1655 | |
EcoGene:EG10767 |
Escherichia coli str. K-12 substr. MG1655 | |
Escherichia coli str. K-12 substr. MG1655 | ||
RegulonDB:ECK120000758 |
Escherichia coli str. K-12 substr. MG1655 | |
EchoBASE:EB0760 |
Escherichia coli str. K-12 substr. MG1655 | |
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Notes
Links
Name | URL | Comments |
---|---|---|
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Expression
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Overview
This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.
Cellular Levels
Molecule | Organism or Strain | Value | Units | Experimental Conditions | Assay used | Notes | Reference(s) |
---|---|---|---|---|---|---|---|
Protein |
E. coli K-12 MC4100 |
4.53E+02 |
molecules/cell |
|
emPAI |
PMID:18304323 | |
Protein |
Ecoli K-12 |
50.991+/-0.327 |
Molecules/cell |
|
Single Molecule Fluorescence |
PMID:20671182 | |
mRNA |
Ecoli K-12 |
0.139664804 |
Molecules/cell |
|
by RNA_Seq |
PMID:20671182 | |
Protein |
E. coli K-12 MG1655 |
2289 |
molecules/cell/generation |
|
Ribosome Profiling |
PMID: 24766808 | |
Protein |
E. coli K-12 MG1655 |
803 |
molecules/cell/generation |
|
Ribosome Profiling |
PMID: 24766808 | |
Protein |
E. coli K-12 MG1655 |
1705 |
molecules/cell/generation |
|
Ribosome Profiling |
PMID: 24766808 | |
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Notes
Transcription and Transcriptional Regulation
<protect>
See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.
|
</protect>
Notes
This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.
Translation and Regulation of Translation
<protect><gbrowseImage>
name=NC_000913:260707..260747
source=MG1655
type=Gene+DNA_+Protein
preset=Nterminus
</gbrowseImage>
This picture shows the sequence around the N-terminus.
</protect>
Notes
This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.
Turnover and Regulation of Turnover
</protect>
Notes
This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.
Experimental
<protect>
Mutations Affecting Expression
See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.
Allele Name | Mutation | Phenotype | Reference |
---|---|---|---|
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Expression Studies
See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.
Type | Reference | Notes |
---|---|---|
microarray |
NCBI GEO profiles for proA | |
microarray |
Summary of data for proA from multiple microarray studies | |
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Expression Resources
See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.
Resource Name | Resource Type | Description | Source | Notes |
---|---|---|---|---|
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Notes
Accessions Related to proA Expression
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
Database | Accession | Notes |
---|---|---|
EcoCyc:EG10767 |
Escherichia coli str. K-12 substr. MG1655 | |
EchoBASE:EB0760 |
Escherichia coli str. K-12 substr. MG1655 | |
EcoGene:EG10767 |
Escherichia coli str. K-12 substr. MG1655 | |
RegulonDB:ECK120000758 |
Escherichia coli str. K-12 substr. MG1655 | |
ASAP:ABE-0000832 |
Escherichia coli str. K-12 substr. MG1655 | |
edit table |
<protect></protect>
Notes
Links
Name | URL | Comments |
---|---|---|
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Evolution
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Homologs in Other Organisms
See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.
Organism | Homologs (Statistics) | Comments |
---|---|---|
Anopheles gambiae |
|
From Inparanoid:20070104 |
Apis mellifera |
|
From Inparanoid:20070104 |
Arabidopsis thaliana |
|
From Inparanoid:20070104 |
Bos taurus |
|
From Inparanoid:20070104 |
Caenorhabditis briggsae |
|
From Inparanoid:20070104 |
Caenorhabditis elegans |
|
From Inparanoid:20070104 |
Canis familiaris |
|
From Inparanoid:20070104 |
Ciona intestinalis |
|
From Inparanoid:20070104 |
Drosophila melanogaster |
|
From Inparanoid:20070104 |
Drosophila pseudoobscura |
|
From Inparanoid:20070104 |
Gallus gallus |
|
From Inparanoid:20070104 |
Homo sapiens |
|
From Inparanoid:20070104 |
Macaca mulatta |
|
From Inparanoid:20070104 |
Monodelphis domestica |
|
From Inparanoid:20070104 |
Mus musculus |
|
From Inparanoid:20070104 |
Oryza gramene |
|
From Inparanoid:20070104 |
Pan troglodytes |
|
From Inparanoid:20070104 |
Rattus norvegicus |
|
From Inparanoid:20070104 |
Saccharomyces cerevisiae |
|
From Inparanoid:20070104 |
Schizosaccharomyces pombe |
|
From Inparanoid:20070104 |
Takifugu rubripes |
|
From Inparanoid:20070104 |
Tetraodon nigroviridis |
|
From Inparanoid:20070104 |
Xenopus tropicalis |
|
From Inparanoid:20070104 |
Shigella flexneri |
PROA |
From SHIGELLACYC |
E. coli O157 |
PROA |
From ECOO157CYC |
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Notes
Families
<protect>
See Help:Evolution_families for help entering or editing information in this section of EcoliWiki.
Database | Accession | Notes |
---|---|---|
| ||
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</protect> <protect></protect>
Notes
Links
Name | URL | Comments |
---|---|---|
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 1.8 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ 3.0 3.1 3.2 3.3 3.4 3.5 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ 4.0 4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
- ↑ 5.0 5.1 5.2 5.3 CGSC: The Coli Genetics Stock Center
- ↑ 6.0 6.1 6.2 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
Categories
- Genes with homologs in Anopheles gambiae
- Genes with homologs in Apis mellifera
- Genes with homologs in Arabidopsis thaliana
- Genes with homologs in Bos taurus
- Genes with homologs in Caenorhabditis briggsae
- Genes with homologs in Caenorhabditis elegans
- Genes with homologs in Canis familiaris
- Genes with homologs in Ciona intestinalis
- Genes with homologs in Drosophila melanogaster
- Genes with homologs in Drosophila pseudoobscura
- Genes with homologs in Gallus gallus
- Genes with homologs in Homo sapiens
- Genes with homologs in Macaca mulatta
- Genes with homologs in Monodelphis domestica
- Genes with homologs in Mus musculus
- Genes with homologs in Oryza gramene
- Genes with homologs in Pan troglodytes
- Genes with homologs in Rattus norvegicus
- Genes with homologs in Saccharomyces cerevisiae
- Genes with homologs in Schizosaccharomyces pombe
- Genes with homologs in Takifugu rubripes
- Genes with homologs in Tetraodon nigroviridis
- Genes with homologs in Xenopus tropicalis
- Genes with homologs in Shigella flexneri
- Genes with homologs in E. coli O157