lon:Gene
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| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Location(s) and DNA Sequence | Sequence Features | Alleles and Phenotypes | Genetic Interactions | Genetic Resources | Accessions | Links | References | Suggestions |
Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
| Standard name |
lon |
|---|---|
| Mnemonic |
Long form |
| Synonyms |
ECK0433, b0439, JW0429, capR, deg, dir, muc, lopA[1], lonA, lopA |
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Notes
Location(s) and DNA Sequence
See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
|
MG1655 |
9.87 minutes |
MG1655: 458112..460466 |
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NC_012967: 430943..433297 |
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NC_012759: 360871..363225 |
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W3110 |
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W3110: 458112..460466 |
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DH10B: 397443..399797 |
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Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
| Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
|---|---|---|---|---|---|
|
Coding Start (SO:0000323) |
458112 |
Edman degradation |
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Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
| Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
|---|---|---|---|---|---|---|---|
|
Δlon (Keio:JW0429) |
deletion |
deletion |
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lon::Tn5KAN-2 (FB20175) |
Insertion at nt 1865 in Minus orientation |
does not contain pKD46 | |||||
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lonK362A |
K362A |
Loss of proteolytic activity and ATP-binding |
seeded from UniProt:P0A9M0 | ||||
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lonS679A |
S679A |
Loss of proteolytic activity |
seeded from UniProt:P0A9M0 | ||||
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Δlon::kan |
deletion |
Biolog:respiration |
unable to respire Dextrin |
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Δlon::kan |
deletion |
Biolog:respiration |
unable to respire D-Mannose |
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Δlon::kan |
deletion |
Biolog:respiration |
unable to respire b-Methyl-D-glucoside |
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Δlon::kan |
deletion |
Biolog:respiration |
unable to respire DL-a-Glycerol |
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Δlon::kan |
deletion |
Biolog:respiration |
unable to respire D-Galactoniate |
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Δlon::kan |
deletion |
Biolog:respiration |
unable to respire D-Galacturonate |
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Δlon::kan |
deletion |
Biolog:respiration |
unable to respire D-Glucuronate |
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Δlon::kan |
deletion |
Biolog:respiration |
unable to respire Acetate |
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Δlon::kan |
deletion |
Biolog:respiration |
unable to respire a-Hydroxybutyrate |
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Δlon::kan |
deletion |
Biolog:respiration |
unable to respire a-Ketobutyrate |
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Δlon::kan |
deletion |
Biolog:respiration |
unable to respire a-Ketoglutarate |
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Δlon::kan |
deletion |
Biolog:respiration |
unable to respire DL-Lactate |
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Δlon::kan |
deletion |
Biolog:respiration |
unable to respire Succinate |
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Δlon::kan |
deletion |
Biolog:respiration |
unable to respire Bromosuccinate |
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Δlon::kan |
deletion |
Biolog:respiration |
unable to respire Glucuronamide |
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Δlon::kan |
deletion |
Biolog:respiration |
unable to respire L-Alanine |
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Δlon::kan |
deletion |
Biolog:respiration |
unable to respire L-Alanyl-glycine |
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Δlon::kan |
deletion |
Biolog:respiration |
unable to respire L-Asparagine |
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Δlon::kan |
deletion |
Biolog:respiration |
unable to respire L-Aspartate |
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Δlon::kan |
deletion |
Biolog:respiration |
unable to respire Glycyl-L-aspartate |
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Δlon::kan |
deletion |
Biolog:respiration |
unable to respire L-Serine |
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Δlon::kan |
deletion |
Biolog:respiration |
unable to respire Uridine |
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|
lon-11 |
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lon-21 |
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lon-22 |
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lon-1::IS186 |
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lon-20 |
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lon-10 |
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lon-6 |
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lon-7 |
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lon-9 |
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lon-201 |
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lon-202 |
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Δlon-725::kan |
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capR9 |
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capR9 |
derepression of UDPG pyrophosphorylase |
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|
lon |
deletion |
Sensitivity to |
increases sensitivity to bicyclomycin |
fig 2 | |||
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Notes
Genetic Interactions
| Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
|---|---|---|---|---|---|---|
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Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
|
JW0429 |
Plasmid clone |
Status:Clone OK Primer 1:GCCAATCCTGAGCGTTCTGAACG Primer 2:CCTTTTGCAGTCACAACCTGCAT | |
|
Kohara Phage |
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|
Linked marker |
est. P1 cotransduction: 36% [13] | ||
|
Linked marker |
est. P1 cotransduction: 96% [13] | ||
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
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Notes
Links
| Name | URL | Comments |
|---|---|---|
| edit table |
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ Gayda, RC et al. (1985) Regulatory region of the heat shock-inducible capR (lon) gene: DNA and protein sequences. J. Bacteriol. 162 271-5 PubMed
- ↑ Thomas, CD et al. (1993) Controlled high-level expression of the lon gene of Escherichia coli allows overproduction of Lon protease. Gene 136 237-42 PubMed
- ↑ 4.0 4.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
- ↑ 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
- ↑ Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
- ↑ 7.00 7.01 7.02 7.03 7.04 7.05 7.06 7.07 7.08 7.09 7.10 7.11 7.12 7.13 7.14 7.15 7.16 7.17 7.18 7.19 7.20 7.21 Ito, M et al. (2005) Functional analysis of 1440 Escherichia coli genes using the combination of knock-out library and phenotype microarrays. Metab. Eng. 7 318-27 PubMed
- ↑ Bush, JW & Markovitz, A (1973) The Genetic Basis for Mucoidy and Radiation Sensitivity in capR (lon) Mutants of E. coli K-12. Genetics 74 215-25 PubMed
- ↑ Lieberman, MM et al. (1970) Derepression of GDP-alpha-mannose and UDP-glucose pyrophosphorylases by a regulator gene mutation; episomal dominance in partial diploids. Proc. Natl. Acad. Sci. U.S.A. 65 625-32 PubMed
- ↑ Tran, L et al. (2011) Single-gene deletion mutants of Escherichia coli with altered sensitivity to bicyclomycin, an inhibitor of transcription termination factor Rho. J. Bacteriol. 193 2229-35 PubMed
- ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
- ↑ Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
- ↑ 13.0 13.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).