PMID:4910851
| Citation |
Lieberman, MM, Buchanan, CE and Markovitz, A (1970) Derepression of GDP-alpha-mannose and UDP-glucose pyrophosphorylases by a regulator gene mutation; episomal dominance in partial diploids. Proc. Natl. Acad. Sci. U.S.A. 65:625-32 |
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| Abstract |
Mutants of Escherichia coli K12 at the capR locus are overproduce capsular polysaccharide, and are derepressed for synthesis of several enzymes involved in capsular polysaccharide synthesis(1-3) including GDP-mannose pyrophosphorylase.(8) UDP-glucose pyrophosphorylase is also derepressed in a haploid capR9 mucoid mutant. Heterozygous mucoid partial diploids with the capR9 allele on the episome and the wild-type (capR(+)) allele on the chromosome (F'capR9/capR(+)) are derepressed for UDP-glucose pyrophosphorylase and GDP-mannose pyrophosphorylase, while the reciprocal nonmucoid heterozygotes (F'capR(+)/capR9) are repressed for these enzymes. These results provide evidence that the episomal capR allele is dominant with respect to synthesis of these two enzymes. |
| Links | |
| Keywords |
Diploidy; Enzyme Repression; Escherichia coli/enzymology; Genes, Dominant; Genes, Regulator; Haploidy; Mutation; Nucleotidyltransferases/biosynthesis; Polysaccharides, Bacterial/biosynthesis |
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