lon:Gene Product(s)

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Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

Lon

Synonyms

DNA-binding ATP-dependent protease La[1], B0439[2][1], Muc[2][1], Dir[2][1], Deg[2][1], LopA[2][1], CapR[2][1], Lon[2][1] , capR, deg, dir, ECK0433, JW0429, lonA, lopA, muc, b0439

Product description

Lon[2][3];

Component of DNA-binding, ATP-dependent protease La[3]

DNA-binding, ATP-dependent protease LA; lon mutants form long cells[4]

EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0000166

nucleotide binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR003593

F

Seeded from EcoCyc (v14.0)

complete

GO:0000166

nucleotide binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0547

F

Seeded from EcoCyc (v14.0)

complete

GO:0003677

DNA binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0238

F

Seeded from EcoCyc (v14.0)

complete

GO:0004176

ATP-dependent peptidase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001984

F

Seeded from EcoCyc (v14.0)

complete

GO:0004176

ATP-dependent peptidase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR003111

F

Seeded from EcoCyc (v14.0)

complete

GO:0004176

ATP-dependent peptidase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR003111
InterPro:IPR004815

F

Seeded from EcoCyc (v14.0)

complete

GO:0004176

ATP-dependent peptidase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004815

F

Seeded from EcoCyc (v14.0)

complete

GO:0004176

ATP-dependent peptidase activity

PMID:6458036[5]

IDA: Inferred from Direct Assay

F

complete

GO:0004176

ATP-dependent peptidase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR008268

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

PMID:15037242[6]

IPI: Inferred from Physical Interaction

F

Missing: with/from

GO:0004176

ATP-dependent peptidase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR008269

F

Seeded from EcoCyc (v14.0)

complete

GO:0016887

ATPase activity

PMID:6458036[5]

IDA: Inferred from Direct Assay

F

complete

GO:0004252

serine-type endopeptidase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001984

F

Seeded from EcoCyc (v14.0)

complete

GO:0008233

peptidase activity

PMID:9720920[7]

IDA: Inferred from Direct Assay

F

complete

GO:0004252

serine-type endopeptidase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR008268

F

Seeded from EcoCyc (v14.0)

complete

GO:0006508

proteolysis

PMID:6458036[5]

IDA: Inferred from Direct Assay

P

complete

GO:0004176

ATP-dependent peptidase activity

PMID:9720920[7]

IDA: Inferred from Direct Assay

F

complete

GO:0004252

serine-type endopeptidase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR008269

F

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

PMID:8995294[8]

IDA: Inferred from Direct Assay

C

complete

GO:0005524

ATP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR003959

F

Seeded from EcoCyc (v14.0)

complete

GO:0003677

DNA binding

PMID:8995294[8]

IDA: Inferred from Direct Assay

F

complete

GO:0005524

ATP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004815

F

Seeded from EcoCyc (v14.0)

complete

GO:0006950

response to stress

PMID:6330035[9]

IEP: Inferred from Expression Pattern

P

complete

GO:0005524

ATP binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0067

F

Seeded from EcoCyc (v14.0)

complete

GO:0004252

serine-type endopeptidase activity

PMID:15606774[10]

IMP: Inferred from Mutant Phenotype

F

complete

GO:0005737

cytoplasm

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0963

C

Seeded from EcoCyc (v14.0)

complete

GO:0009408

response to heat

PMID:8349564[11]

IEP: Inferred from Expression Pattern

P

complete

GO:0005737

cytoplasm

GO_REF:0000023

IEA: Inferred from Electronic Annotation

SP_SL:SL-0086

C

Seeded from EcoCyc (v14.0)

complete

GO:0006508

proteolysis

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001984

P

Seeded from EcoCyc (v14.0)

complete

GO:0006508

proteolysis

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR003111

P

Seeded from EcoCyc (v14.0)

complete

GO:0006508

proteolysis

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004815

P

Seeded from EcoCyc (v14.0)

complete

GO:0006508

proteolysis

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR008268

P

Seeded from EcoCyc (v14.0)

complete

GO:0006508

proteolysis

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR008269

P

Seeded from EcoCyc (v14.0)

complete

GO:0006950

response to stress

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0346

P

Seeded from EcoCyc (v14.0)

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

Subunits of DNA-binding, ATP-dependent protease La

could be indirect

Protein

ssb

PMID:15690043[12]

Experiment(s):EBI-894571

Protein

rplC

PMID:15690043[12]

Experiment(s):EBI-889577

Protein

rplI

PMID:15690043[12]

Experiment(s):EBI-889577, EBI-894571

Protein

rplM

PMID:15690043[12]

Experiment(s):EBI-889577, EBI-894571

Protein

rplT

PMID:15690043[12]

Experiment(s):EBI-889577

Protein

rpsB

PMID:15690043[12]

Experiment(s):EBI-889577

Protein

rpsC

PMID:15690043[12]

Experiment(s):EBI-889577

Protein

tufA

PMID:15690043[12]

Experiment(s):EBI-889577

Protein

fsaA

PMID:15690043[12]

Experiment(s):EBI-894571

Protein

rplU

PMID:15690043[12]

Experiment(s):EBI-894571

Protein

rplV

PMID:15690043[12]

Experiment(s):EBI-894571

Protein

rpsG

PMID:15690043[12]

Experiment(s):EBI-894571

Protein

rpsH

PMID:15690043[12]

Experiment(s):EBI-894571

Protein

yjgL

PMID:15690043[12]

Experiment(s):EBI-894571

Protein

dnaX

PMID:16606699[13]

Experiment(s):EBI-1136659

Protein

sapF

PMID:16606699[13]

Experiment(s):EBI-1136659

Protein

secB

PMID:16606699[13]

Experiment(s):EBI-1136659

Protein

dnaA

PMID:16606699[13]

Experiment(s):EBI-1136659

Protein

nadE

PMID:16606699[13]

Experiment(s):EBI-1136659

Protein

ssb

PMID:19402753[14]

LCMS(ID Probability):99.6 MALDI(Z-score):2.272167

Protein

rpsC

PMID:19402753[14]

MALDI(Z-score):31.921524

Protein

rplI

PMID:19402753[14]

LCMS(ID Probability):99.0 MALDI(Z-score):17.053160

Protein

rplU

PMID:19402753[14]

LCMS(ID Probability):99.6

Protein

yjgL

PMID:19402753[14]

LCMS(ID Probability):99.6

Protein

rplM

PMID:19402753[14]

LCMS(ID Probability):99.6 MALDI(Z-score):28.085510

Protein

rplA

PMID:19402753[14]

MALDI(Z-score):23.969367

Protein

clpA

PMID:19402753[14]

MALDI(Z-score):22.779291

Protein

tufB

PMID:19402753[14]

MALDI(Z-score):22.916243

Small Molecule

Diisopropyl fluorophosphate

PMID:6458037[15]

Small Molecule

Mg2+

PMID:6458037[15]

Data was not shown, but indicated that the presence of both Mg2+ and ATP were required for proteolytic activity.

Small Molecule

ATP

PMID:6458037[15]

See evidence for Mg2+

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Notes

For a review of Lon and other proteases, see Proteases and their targets in Escherichia coli. Gottesman (1996)[16]

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MNPERSERIE IPVLPLRDVV VYPHMVIPLF VGREKSIRCL EAAMDHDKKI MLVAQKEAST
DEPGVNDLFT VGTVASILQM LKLPDGTVKV LVEGLQRARI SALSDNGEHF SAKAEYLESP
TIDEREQEVL VRTAISQFEG YIKLNKKIPP EVLTSLNSID DPARLADTIA AHMPLKLADK
QSVLEMSDVN ERLEYLMAMM ESEIDLLQVE KRIRNRVKKQ MEKSQREYYL NEQMKAIQKE
LGEMDDAPDE NEALKRKIDA AKMPKEAKEK AEAELQKLKM MSPMSAEATV VRGYIDWMVQ
VPWNARSKVK KDLRQAQEIL DTDHYGLERV KDRILEYLAV QSRVNKIKGP ILCLVGPPGV
GKTSLGQSIA KATGRKYVRM ALGGVRDEAE IRGHRRTYIG SMPGKLIQKM AKVGVKNPLF
LLDEIDKMSS DMRGDPASAL LEVLDPEQNV AFSDHYLEVD YDLSDVMFVA TSNSMNIPAP
LLDRMEVIRL SGYTEDEKLN IAKRHLLPKQ IERNALKKGE LTVDDSAIIG IIRYYTREAG
VRGLEREISK LCRKAVKQLL LDKSLKHIEI NGDNLHDYLG VQRFDYGRAD NENRVGQVTG
LAWTEVGGDL LTIETACVPG KGKLTYTGSL GEVMQESIQA ALTVVRARAE KLGINPDFYE
KRDIHVHVPE GATPKDGPSA GIAMCTALVS CLTGNPVRAD VAMTGEITLR GQVLPIGGLK
EKLLAAHRGG IKTVLIPFEN KRDLEEIPDN VIADLDIHPV KRIEEVLTLA LQNEPSGMQV VTAK
Length

784

Mol. Wt

87.44 kDa

pI

6.2 (calculated)

Extinction coefficient

46,300 - 47,050 (calc based on 20 Y, 3 W, and 6 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

352..493

PF00004 ATPase family associated with various cellular activities (AAA)

PMID:19920124[17]

Domain

10..202

PF02190 ATP-dependent protease La (LON) domain

PMID:19920124[17]

Domain

569..773

PF05362 Lon protease (S16) C-terminal proteolytic domain

PMID:19920124[17]

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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=lon taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16128424

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:945085

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0001521

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P0A9M0

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10542

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10542

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:945085

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000535

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0537

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 1.8 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 2.4 2.5 2.6 2.7 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 5.2 Charette, MF et al. (1981) ATP hydrolysis-dependent protease activity of the lon (capR) protein of Escherichia coli K-12. Proc. Natl. Acad. Sci. U.S.A. 78 4728-32 PubMed
  6. ↑ Botos, I et al. () Crystal structure of the AAA+ alpha domain of E. coli Lon protease at 1.9A resolution. J. Struct. Biol. 146 113-22 PubMed
  7. ↑ 7.0 7.1 Rasulova, FS et al. (1998) The isolated proteolytic domain of Escherichia coli ATP-dependent protease Lon exhibits the peptidase activity. FEBS Lett. 432 179-81 PubMed
  8. ↑ 8.0 8.1 Fu, GK et al. (1997) Bacterial protease Lon is a site-specific DNA-binding protein. J. Biol. Chem. 272 534-8 PubMed
  9. ↑ Phillips, TA et al. (1984) lon gene product of Escherichia coli is a heat-shock protein. J. Bacteriol. 159 283-7 PubMed
  10. ↑ Rotanova, TV et al. (2004) Classification of ATP-dependent proteases Lon and comparison of the active sites of their proteolytic domains. Eur. J. Biochem. 271 4865-71 PubMed
  11. ↑ Chuang, SE & Blattner, FR (1993) Characterization of twenty-six new heat shock genes of Escherichia coli. J. Bacteriol. 175 5242-52 PubMed
  12. ↑ 12.00 12.01 12.02 12.03 12.04 12.05 12.06 12.07 12.08 12.09 12.10 12.11 12.12 12.13 Butland, G et al. (2005) Interaction network containing conserved and essential protein complexes in Escherichia coli. Nature 433 531-7 PubMed
  13. ↑ 13.0 13.1 13.2 13.3 13.4 Arifuzzaman, M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res. 16 686-91 PubMed
  14. ↑ 14.0 14.1 14.2 14.3 14.4 14.5 14.6 14.7 14.8 Hu, P et al. (2009) Global functional atlas of Escherichia coli encompassing previously uncharacterized proteins. PLoS Biol. 7 e96 PubMed
  15. ↑ 15.0 15.1 15.2 Chung, CH & Goldberg, AL (1981) The product of the lon (capR) gene in Escherichia coli is the ATP-dependent protease, protease La. Proc. Natl. Acad. Sci. U.S.A. 78 4931-5 PubMed
  16. ↑ Gottesman, S (1996) Proteases and their targets in Escherichia coli. Annu. Rev. Genet. 30 465-506 PubMed
  17. ↑ 17.0 17.1 17.2 Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed

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