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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Quickview | Gene | Product(s) | Expression| Evolution | References |

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<protect>

Standard Name

lon

Gene Synonym(s)

ECK0433, b0439, JW0429, capR, deg, dir, muc, lopA[1], lonA, lopA

Product Desc.

Lon[2][3];

Component of DNA-binding, ATP-dependent protease La[3]

DNA-binding, ATP-dependent protease LA; lon mutants form long cells[4]

Product Synonyms(s)

DNA-binding ATP-dependent protease La[1], B0439[2][1], Muc[2][1], Dir[2][1], Deg[2][1], LopA[2][1], CapR[2][1], Lon[2][1] , capR, deg, dir, ECK0433, JW0429, lonA, lopA, muc, b0439

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): lon[2]

Regulation/Activity
Quick Links

porteco.png EcoCyc.gif regulondb.jpg

dnadisplay.png proteindisplay.png   pubmed.jpg   textpresso.jpg  

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Notes

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Lon dependent lethality is due to induction of yefM-yoeb toxin/antitoxin pair.[4]



Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

lon

Mnemonic

Long form

Synonyms

ECK0433, b0439, JW0429, capR, deg, dir, muc, lopA[1], lonA, lopA

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

9.87 minutes 

MG1655: 458112..460466
<gbrowseImage> name=NC_000913:458112..460466 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 430943..433297
<gbrowseImage> name=NC_012967:430943..433297 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 360871..363225
<gbrowseImage> name=NC_012759:360871..363225 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 458112..460466
<gbrowseImage> name=NC_007779:458112..460466 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 397443..399797
<gbrowseImage> name=NC_010473:397443..399797 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

458112

Edman degradation

PMID:2984174
PMID:8294008


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

lon(del) (Keio:JW0429)

deletion

deletion

PMID:16738554

Shigen

CGSC8592[5]

lon::Tn5KAN-2 (FB20175)

Insertion at nt 1865 in Minus orientation

PMID:15262929

E. coli Genome Project:FB20175

does not contain pKD46

lonK362A

K362A

Loss of proteolytic activity and ATP-binding

seeded from UniProt:P0A9M0

lonS679A

S679A

Loss of proteolytic activity

seeded from UniProt:P0A9M0

lon(del)::kan

deletion

Biolog:respiration

unable to respire Dextrin

PMID:16095938

lon(del)::kan

deletion

Biolog:respiration

unable to respire D-Mannose

PMID:16095938

lon(del)::kan

deletion

Biolog:respiration

unable to respire b-Methyl-D-glucoside

PMID:16095938

lon(del)::kan

deletion

Biolog:respiration

unable to respire DL-a-Glycerol

PMID:16095938

lon(del)::kan

deletion

Biolog:respiration

unable to respire D-Galactoniate

PMID:16095938

lon(del)::kan

deletion

Biolog:respiration

unable to respire D-Galacturonate

PMID:16095938

lon(del)::kan

deletion

Biolog:respiration

unable to respire D-Glucuronate

PMID:16095938

lon(del)::kan

deletion

Biolog:respiration

unable to respire Acetate

PMID:16095938

lon(del)::kan

deletion

Biolog:respiration

unable to respire a-Hydroxybutyrate

PMID:16095938

lon(del)::kan

deletion

Biolog:respiration

unable to respire a-Ketobutyrate

PMID:16095938

lon(del)::kan

deletion

Biolog:respiration

unable to respire a-Ketoglutarate

PMID:16095938

lon(del)::kan

deletion

Biolog:respiration

unable to respire DL-Lactate

PMID:16095938

lon(del)::kan

deletion

Biolog:respiration

unable to respire Succinate

PMID:16095938

lon(del)::kan

deletion

Biolog:respiration

unable to respire Bromosuccinate

PMID:16095938

lon(del)::kan

deletion

Biolog:respiration

unable to respire Glucuronamide

PMID:16095938

lon(del)::kan

deletion

Biolog:respiration

unable to respire L-Alanine

PMID:16095938

lon(del)::kan

deletion

Biolog:respiration

unable to respire L-Alanyl-glycine

PMID:16095938

lon(del)::kan

deletion

Biolog:respiration

unable to respire L-Asparagine

PMID:16095938

lon(del)::kan

deletion

Biolog:respiration

unable to respire L-Aspartate

PMID:16095938

lon(del)::kan

deletion

Biolog:respiration

unable to respire Glycyl-L-aspartate

PMID:16095938

lon(del)::kan

deletion

Biolog:respiration

unable to respire L-Serine

PMID:16095938

lon(del)::kan

deletion

Biolog:respiration

unable to respire Uridine

PMID:16095938

lon-11

CGSC:4788

lon-21

CGSC:5770

lon-22

CGSC:6957

lon-1::IS186

CGSC:7591

lon-20

CGSC:7652

lon-10

CGSC:7752

lon-6

CGSC:9311

lon-7

CGSC:9313

lon-9

CGSC:11065

lon-201

CGSC:19616

lon-202

CGSC:19617

lon-725(del)::kan

PMID:16738554

CGSC:101680

capR9

  • mucoidy
  • radiation sensitivity

PMID:17248613

capR9

derepression of UDPG pyrophosphorylase

PMID:4910851

lon

deletion

Sensitivity to

increases sensitivity to bicyclomycin

PMID:21357484

fig 2

lon-

Irradiated with UV light.

Growth Phenotype

Filament formation was reduced after being Irradiated.

PMID 5335887

Strain AX14 was used. See Figure 1.

Ion-

Irradiation strain in YET agar plates.

Colony Morphology

Once exposed to UV radiation colony formation by the mutant cells was not observed.

PMID:5335887

See Fig 3. D

lon-

Moved from YET broth to Glucose minimal agar

Growth Phenotype

The Lesions caused by the UV radiation were repaired.

PMID:5335887

See Figure 4.

lon-

Puromycin addition

Protein Synthesis

Puromycin's inhibited L-arginine-UL-C incorporation by AX14 almost immediately, taking 25 minutes to complete

PMID:5335887

See figure 6

lon725(del)::FRT

deletion

Mutagenesis

Decrease in stress induced mutagenesis (SIM).

PMID:23224554

Parent Strain: SMR4562 Experimental Strain: SMR12254

The mutation conferred a decrease in SIM with a reduction in mutant frequency by over 90 percent. See table S3 for full experimental data.

lon725(del)::FRT

deletion

Sensitivity to

SDS-EDTA sensitivity

PMID:23224554

Parent Strain: SMR4562 Experimental Strain: SMR12254

The mutation conferred increased sensitivity toward SDS-EDTA. See table S7 and S1 for summary of experimental data.

lon725(del)::FRT

deletion

Sensitivity to

UV sensitivity

PMID:23224554

Parent Strain: SMR4562 Experimental Strain: SMR12254

The mutation conferred increased sensitivity to UV. See table S7 and S1 for summary of experimental data.

CAG45114 lon725(del)::FRTKanFR

deletion

SigmaE activity

decrease in sigmaE activity

PMID:23224554

Parental Strain: CAG45114 Experimental Strain: SMR15273

See table S11 for full experimental results.

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Notes

Genetic Interactions

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<protect>

Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW0429

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCAATCCTGAGCGTTCTGAACG

Primer 2:CCTTTTGCAGTCACAACCTGCAT

3B6

Kohara Phage

Genobase

PMID:3038334

tsx-247::Tn10

Linked marker

CAG12148 = CGSC7337[5]

est. P1 cotransduction: 36% [6]

zaj-3054::Tn10

Linked marker

CAG12017 = CGSC7339[5]

est. P1 cotransduction: 96% [6]
Synonyms:zba-3054::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10542

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10542

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000535

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:945085

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0537

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0001521

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

Lon

Synonyms

DNA-binding ATP-dependent protease La[1], B0439[2][1], Muc[2][1], Dir[2][1], Deg[2][1], LopA[2][1], CapR[2][1], Lon[2][1] , capR, deg, dir, ECK0433, JW0429, lonA, lopA, muc, b0439

Product description

Lon[2][3];

Component of DNA-binding, ATP-dependent protease La[3]

DNA-binding, ATP-dependent protease LA; lon mutants form long cells[4]

EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status

GO:0000166

nucleotide binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR003593

F

Seeded from EcoCyc (v14.0)

complete

GO:0000166

nucleotide binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0547

F

Seeded from EcoCyc (v14.0)

complete

GO:0003677

DNA binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0238

F

Seeded from EcoCyc (v14.0)

complete

GO:0004176

ATP-dependent peptidase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001984

F

Seeded from EcoCyc (v14.0)

complete

GO:0004176

ATP-dependent peptidase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR003111

F

Seeded from EcoCyc (v14.0)

complete

GO:0004176

ATP-dependent peptidase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR003111
InterPro:IPR004815

F

Seeded from EcoCyc (v14.0)

complete

GO:0004176

ATP-dependent peptidase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004815

F

Seeded from EcoCyc (v14.0)

complete

GO:0004176

ATP-dependent peptidase activity

PMID:6458036

IDA: Inferred from Direct Assay

F

complete

GO:0004176

ATP-dependent peptidase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR008268

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

PMID:15037242

IPI: Inferred from Physical Interaction

F

Missing: with/from

GO:0004176

ATP-dependent peptidase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR008269

F

Seeded from EcoCyc (v14.0)

complete

GO:0016887

ATPase activity

PMID:6458036

IDA: Inferred from Direct Assay

F

complete

GO:0004252

serine-type endopeptidase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001984

F

Seeded from EcoCyc (v14.0)

complete

GO:0008233

peptidase activity

PMID:9720920

IDA: Inferred from Direct Assay

F

complete

GO:0004252

serine-type endopeptidase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR008268

F

Seeded from EcoCyc (v14.0)

complete

GO:0006508

proteolysis

PMID:6458036

IDA: Inferred from Direct Assay

P

complete

GO:0004176

ATP-dependent peptidase activity

PMID:9720920

IDA: Inferred from Direct Assay

F

complete

GO:0004252

serine-type endopeptidase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR008269

F

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

PMID:8995294

IDA: Inferred from Direct Assay

C

complete

GO:0005524

ATP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR003959

F

Seeded from EcoCyc (v14.0)

complete

GO:0003677

DNA binding

PMID:8995294

IDA: Inferred from Direct Assay

F

complete

GO:0005524

ATP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004815

F

Seeded from EcoCyc (v14.0)

complete

GO:0006950

response to stress

PMID:6330035

IEP: Inferred from Expression Pattern

P

complete

GO:0005524

ATP binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0067

F

Seeded from EcoCyc (v14.0)

complete

GO:0004252

serine-type endopeptidase activity

PMID:15606774

IMP: Inferred from Mutant Phenotype

F

complete

GO:0005737

cytoplasm

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0963

C

Seeded from EcoCyc (v14.0)

complete

GO:0009408

response to heat

PMID:8349564

IEP: Inferred from Expression Pattern

P

complete

GO:0005737

cytoplasm

GO_REF:0000023

IEA: Inferred from Electronic Annotation

SP_SL:SL-0086

C

Seeded from EcoCyc (v14.0)

complete

GO:0006508

proteolysis

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001984

P

Seeded from EcoCyc (v14.0)

complete

GO:0006508

proteolysis

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR003111

P

Seeded from EcoCyc (v14.0)

complete

GO:0006508

proteolysis

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004815

P

Seeded from EcoCyc (v14.0)

complete

GO:0006508

proteolysis

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR008268

P

Seeded from EcoCyc (v14.0)

complete

GO:0006508

proteolysis

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR008269

P

Seeded from EcoCyc (v14.0)

complete

GO:0006950

response to stress

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0346

P

Seeded from EcoCyc (v14.0)

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

Subunits of DNA-binding, ATP-dependent protease La

could be indirect

Protein

ssb

PMID:15690043

Experiment(s):EBI-894571

Protein

rplC

PMID:15690043

Experiment(s):EBI-889577

Protein

rplI

PMID:15690043

Experiment(s):EBI-889577, EBI-894571

Protein

rplM

PMID:15690043

Experiment(s):EBI-889577, EBI-894571

Protein

rplT

PMID:15690043

Experiment(s):EBI-889577

Protein

rpsB

PMID:15690043

Experiment(s):EBI-889577

Protein

rpsC

PMID:15690043

Experiment(s):EBI-889577

Protein

tufA

PMID:15690043

Experiment(s):EBI-889577

Protein

fsaA

PMID:15690043

Experiment(s):EBI-894571

Protein

rplU

PMID:15690043

Experiment(s):EBI-894571

Protein

rplV

PMID:15690043

Experiment(s):EBI-894571

Protein

rpsG

PMID:15690043

Experiment(s):EBI-894571

Protein

rpsH

PMID:15690043

Experiment(s):EBI-894571

Protein

yjgL

PMID:15690043

Experiment(s):EBI-894571

Protein

dnaX

PMID:16606699

Experiment(s):EBI-1136659

Protein

sapF

PMID:16606699

Experiment(s):EBI-1136659

Protein

secB

PMID:16606699

Experiment(s):EBI-1136659

Protein

dnaA

PMID:16606699

Experiment(s):EBI-1136659

Protein

nadE

PMID:16606699

Experiment(s):EBI-1136659

Protein

ssb

PMID:19402753

LCMS(ID Probability):99.6 MALDI(Z-score):2.272167

Protein

rpsC

PMID:19402753

MALDI(Z-score):31.921524

Protein

rplI

PMID:19402753

LCMS(ID Probability):99.0 MALDI(Z-score):17.053160

Protein

rplU

PMID:19402753

LCMS(ID Probability):99.6

Protein

yjgL

PMID:19402753

LCMS(ID Probability):99.6

Protein

rplM

PMID:19402753

LCMS(ID Probability):99.6 MALDI(Z-score):28.085510

Protein

rplA

PMID:19402753

MALDI(Z-score):23.969367

Protein

clpA

PMID:19402753

MALDI(Z-score):22.779291

Protein

tufB

PMID:19402753

MALDI(Z-score):22.916243

Small Molecule

Diisopropyl fluorophosphate

PMID:6458037

Small Molecule

Mg2+

PMID:6458037

Data was not shown, but indicated that the presence of both Mg2+ and ATP were required for proteolytic activity.

Small Molecule

ATP

PMID:6458037

See evidence for Mg2+

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</protect>

Notes

For a review of Lon and other proteases, see Proteases and their targets in Escherichia coli. Gottesman (1996)[7]

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MNPERSERIE IPVLPLRDVV VYPHMVIPLF VGREKSIRCL EAAMDHDKKI MLVAQKEAST
DEPGVNDLFT VGTVASILQM LKLPDGTVKV LVEGLQRARI SALSDNGEHF SAKAEYLESP
TIDEREQEVL VRTAISQFEG YIKLNKKIPP EVLTSLNSID DPARLADTIA AHMPLKLADK
QSVLEMSDVN ERLEYLMAMM ESEIDLLQVE KRIRNRVKKQ MEKSQREYYL NEQMKAIQKE
LGEMDDAPDE NEALKRKIDA AKMPKEAKEK AEAELQKLKM MSPMSAEATV VRGYIDWMVQ
VPWNARSKVK KDLRQAQEIL DTDHYGLERV KDRILEYLAV QSRVNKIKGP ILCLVGPPGV
GKTSLGQSIA KATGRKYVRM ALGGVRDEAE IRGHRRTYIG SMPGKLIQKM AKVGVKNPLF
LLDEIDKMSS DMRGDPASAL LEVLDPEQNV AFSDHYLEVD YDLSDVMFVA TSNSMNIPAP
LLDRMEVIRL SGYTEDEKLN IAKRHLLPKQ IERNALKKGE LTVDDSAIIG IIRYYTREAG
VRGLEREISK LCRKAVKQLL LDKSLKHIEI NGDNLHDYLG VQRFDYGRAD NENRVGQVTG
LAWTEVGGDL LTIETACVPG KGKLTYTGSL GEVMQESIQA ALTVVRARAE KLGINPDFYE
KRDIHVHVPE GATPKDGPSA GIAMCTALVS CLTGNPVRAD VAMTGEITLR GQVLPIGGLK
EKLLAAHRGG IKTVLIPFEN KRDLEEIPDN VIADLDIHPV KRIEEVLTLA LQNEPSGMQV VTAK
Length

784

Mol. Wt

87.44 kDa

pI

6.2 (calculated)

Extinction coefficient

46,300 - 47,050 (calc based on 20 Y, 3 W, and 6 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

352..493

PF00004 ATPase family associated with various cellular activities (AAA)

PMID:19920124

Domain

10..202

PF02190 ATP-dependent protease La (LON) domain

PMID:19920124

Domain

569..773

PF05362 Lon protease (S16) C-terminal proteolytic domain

PMID:19920124

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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=lon taxon=562,83333 </beststructure>

Models

View models at:

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</protect>

Structure figures

<protect>

</protect>

Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16128424

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:945085

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0001521

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P0A9M0

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10542

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10542

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:945085

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000535

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0537

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

Protein

E. coli K-12 MC4100

1.55E+03

molecules/cell

  • Medium: median data from rich and minimal media
  • Temperature (°C): 37
  • Doubling time (min): Growth Rate(min):
  • Growth phase: other:

emPAI

PMID:18304323

Protein

Ecoli K-12

158.851+/-0.689

Molecules/cell

  • Medium:
  • Temperature (°C):
  • Doubling time (min): Growth Rate(min): *
  • Growth phase: other: *

Single Molecule Fluorescence

PMID:20671182

mRNA

Ecoli K-12

0.11108+/-0.01107

Molecules/cell

  • Medium:
  • Temperature (°C):
  • Doubling time (min): Growth Rate(min): *
  • Growth phase: other: *

by FISH

PMID:20671182

mRNA

Ecoli K-12

0.204757859

Molecules/cell

  • Medium:
  • Temperature (°C):
  • Doubling time (min): Growth Rate(min): *
  • Growth phase: other: *

by RNA_Seq

PMID:20671182

Protein

E. coli K-12 MG1655

5477

molecules/cell/generation

  • Medium: MOPS Complete
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

1485

molecules/cell/generation

  • Medium: MOPS Minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

4688

molecules/cell/generation

  • Medium: MOPS Complete without Methionine
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

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Notes

Transcription and Transcriptional Regulation

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<protect>

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

lon

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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:458092..458132 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GEO Profiles:b0439 (EcoliWiki Page)

NCBI GEO profiles for lon

microarray

GenExpDB:b0439 (EcoliWiki Page)

Summary of data for lon from multiple microarray studies

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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes

GFP Fusion

Intergenic region (457843..458176) fused to gfpmut2.

OpenBioSystems

GFP fusion described in Zaslaver, et al.
Plate:109-AZ20; Well:G9[8]

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Notes

Accessions Related to lon Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10542

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0537

Escherichia coli str. K-12 substr. MG1655

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b0439

EcoGene

EcoGene:EG10542

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000535

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0001521

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism Homologs (Statistics) Comments

Anopheles gambiae

  • ENSANGP00000013687 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Apis mellifera

  • ENSAPMP00000030012 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Arabidopsis thaliana

  • AT5G47040 (score: 1.000; bootstrap: 77%)

From Inparanoid:20070104

Bos taurus

  • ENSBTAP00000002350 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Caenorhabditis briggsae

  • WBGene00033694 (score: 1.000; bootstrap: 86%)

From Inparanoid:20070104

Caenorhabditis elegans

  • WBGene00016391 (score: 1.000; bootstrap: 90%)

From Inparanoid:20070104

Canis familiaris

  • ENSCAFP00000014765 (score: 1.000; bootstrap: 54%)

From Inparanoid:20070104

Ciona intestinalis

  • ENSCINP00000018656 (score: 1.000; bootstrap: 83%)

From Inparanoid:20070104

Danio rerio

  • ZDB-GENE-041212-1 (score: 1.000; bootstrap: 100%)
  • ZDB-CDNA-041212-2 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Dictyostelium discoideum

  • DDB0204395 (score: 1.000; bootstrap: 100%)
  • DDB0217928 (score: 0.072)

From Inparanoid:20070104

Drosophila melanogaster

  • FBgn0036892 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Drosophila pseudoobscura

  • GA21329-PA (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Gallus gallus

  • ENSGALP00000006273 (score: 1.000; bootstrap: 69%)

From Inparanoid:20070104

Homo sapiens

  • ENSP00000285737 (score: 1.000; bootstrap: 51%)

From Inparanoid:20070104

Macaca mulatta

  • ENSMMUP00000009707 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Monodelphis domestica

  • ENSMODP00000001578 (score: 1.000; bootstrap: 57%)

From Inparanoid:20070104

Mus musculus

  • MGI:1914137 (score: 1.000; bootstrap: 56%)

From Inparanoid:20070104

Oryza gramene

  • P93647 (score: 1.000; bootstrap: 64%)
  • Q94F60 (score: 0.940)
  • Q8GV57 (score: 0.916)
  • Q69SH2 (score: 0.902)
  • Q6RS97 (score: 0.896)

From Inparanoid:20070104

Pan troglodytes

  • ENSPTRP00000013818 (score: 1.000; bootstrap: 99%)

From Inparanoid:20070104

Rattus norvegicus

  • ENSRNOP00000020770 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Saccharomyces cerevisiae

  • YBL022C (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Schizosaccharomyces pombe

  • SPAC22F36c (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Takifugu rubripes

  • NEWSINFRUP00000129607 (score: 1.000; bootstrap: 92%)

From Inparanoid:20070104

Tetraodon nigroviridis

  • GSTENP00005077001 (score: 1.000; bootstrap: 99%)

From Inparanoid:20070104

Xenopus tropicalis

  • ENSXETP00000017719 (score: 1.000; bootstrap: 64%)

From Inparanoid:20070104

Shigella flexneri

LON

From SHIGELLACYC

E. coli O157

LON

From ECOO157CYC

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Do-It-Yourself Web Tools

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Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

Pfam (EcoliWiki Page)

PF02190 ATP-dependent protease La (LON) domain

Pfam (EcoliWiki Page)

PF00004 ATPase family associated with various cellular activities (AAA)

Superfamily (EcoliWiki Page)

SUPERFAMILY:52540

Superfamily (EcoliWiki Page)

SUPERFAMILY:54211

Superfamily (EcoliWiki Page)

SUPERFAMILY:88697

Pfam (EcoliWiki Page)

PF05362 Lon protease (S16) C-terminal proteolytic domain

EcoCyc

EcoCyc:EG10542

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10542

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000535

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0537

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0001521

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 1.15 1.16 1.17 1.18 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 2.13 2.14 2.15 2.16 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 3.2 3.3 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ 4.0 4.1 4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
  6. ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  7. ↑ Gottesman, S (1996) Proteases and their targets in Escherichia coli. Annu. Rev. Genet. 30 465-506 PubMed
  8. ↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

Categories

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