rep:Gene: Difference between revisions
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rep | rep in [[MDS42]] | ||
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deletion | |||
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Revision as of 12:57, 29 September 2011
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| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Location(s) and DNA Sequence | Sequence Features | Alleles and Phenotypes | Genetic Interactions | Genetic Resources | Accessions | Links | References | Suggestions |
Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
| Standard name |
rep |
|---|---|
| Mnemonic |
Replicase |
| Synonyms | |
| edit table |
Notes
Location(s) and DNA Sequence
See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
|
MG1655 |
85.32 minutes |
MG1655: 3958700..3960721 |
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NC_012967: 3922479..3924500 |
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NC_012759: 3848369..3850390 |
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W3110 |
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W3110: 3676004..3673983 |
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DH10B: 4057620..4059641 |
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| edit table |
Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
| Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
|---|---|---|---|---|---|
|
Coding Start (SO:0000323) |
3958700 |
Edman degradation |
| ||
| edit table |
Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
| Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
|---|---|---|---|---|---|---|---|
|
Δrep (Keio:JW5604) |
deletion |
deletion |
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rep::Tn5KAN-I-SceI (FB21451) |
Insertion at nt 508 in Minus orientation |
contains pKD46 | |||||
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Δrep::kan |
deletion |
Biolog:respiration |
unable to respire Acetate |
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Δrep::kan |
deletion |
Biolog:respiration |
unable to respire a-Hydroxybutyrate |
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Δrep::kan |
deletion |
Biolog:respiration |
unable to respire a-Ketobutyrate |
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Δrep::kan |
deletion |
Biolog:respiration |
unable to respire a-Ketoglutarate |
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Δrep::kan |
deletion |
Biolog:respiration |
unable to respire Succinate |
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Δrep::kan |
deletion |
Biolog:respiration |
unable to respire Bromosuccinate |
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|
Δrep::kan |
deletion |
Biolog:respiration |
unable to respire L-Alanine |
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Δrep::kan |
deletion |
Biolog:respiration |
unable to respire L-Asparagine |
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Δrep::kan |
deletion |
Biolog:respiration |
unable to respire L-Aspartate |
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|
Δrep::kan |
deletion |
Biolog:respiration |
unable to respire L-Serine |
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rep-5 |
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rep-3 |
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rep-142(ts) |
temperature sensitive |
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[rep-71] |
null allele | ||||||
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Δrep-729::kan |
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|
rep in MDS42 |
deletion |
Sensitivity to |
increased sensitivity to bicyclomycin |
Fig. 2 | |||
| edit table |
Notes
Genetic Interactions
| Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
|---|---|---|---|---|---|---|
| edit table |
Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
|
JW5604 |
Plasmid clone |
Status:cloned as old JW Primer 1:GCCCGTCTAAACCCCGGCCAACA Primer 2:CCTTTCCCTCGTTTTGCCGCCAT | |
|
Kohara Phage |
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Kohara Phage |
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|
Linked marker |
est. P1 cotransduction: 83% [13] | ||
|
metEo-3079::Tn10 |
Linked marker |
est. P1 cotransduction: 10% [13] | |
| edit table |
Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
| edit table |
Notes
Links
| Name | URL | Comments |
|---|---|---|
| edit table |
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ Bialkowska-Hobrzanska, H et al. (1985) Escherichia coli rep gene: identification of the promoter and N terminus of the rep protein. J. Bacteriol. 164 1004-10 PubMed
- ↑ 4.0 4.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
- ↑ Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
- ↑ 6.00 6.01 6.02 6.03 6.04 6.05 6.06 6.07 6.08 6.09 Ito, M et al. (2005) Functional analysis of 1440 Escherichia coli genes using the combination of knock-out library and phenotype microarrays. Metab. Eng. 7 318-27 PubMed
- ↑ Takahashi, S et al. (1979) The rep mutation. VI. Purification and properties of the Escherichia coli rep protein, DNA helicase III. Can. J. Biochem. 57 855-66 PubMed
- ↑ Tessman, I et al. (1982) Relative map location of the rep and rho genes of Escherichia coli. J. Bacteriol. 151 1637-40 PubMed
- ↑ Washburn, RS & Gottesman, ME (2011) Transcription termination maintains chromosome integrity. Proc. Natl. Acad. Sci. U.S.A. 108 792-7 PubMed
- ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
- ↑ 11.0 11.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
- ↑ 12.0 12.1 CGSC: The Coli Genetics Stock Center
- ↑ 13.0 13.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).