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!|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments
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!|Database!!Accession!!Notes
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[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])
[[EcoCyc]]
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[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=EG10837 ECOCYC:EG10837]
EcoCyc:EG10837
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Escherichia coli str. K-12 substr. MG1655  
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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[http://ecogene.org EcoGene] ([[EcoGene|EcoliWiki Page]])
[[EcoGene]]
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[http://ecogene.org/geneinfo.php?eg_id=EG10837 EcoGene:EG10837]
EcoGene:EG10837
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Escherichia coli str. K-12 substr. MG1655  
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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Escherichia coli str. K-12 substr. MG1655  
Escherichia coli str. K-12 substr. MG1655  
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[[RegulonDB]]
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RegulonDB:ECK120000828
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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[[EchoBASE]]
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EchoBASE:EB0830
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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[[ASAP]]
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ASAP:ABE-0012344
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]


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Revision as of 07:32, 27 May 2013

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

rep

Mnemonic

Replicase

Synonyms

ECK3770, b3778, JW5604, dasC, mbrA, mmrA, dasC?[1][2], mmrA?

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

85.32 minutes 

MG1655: 3958700..3960721
<gbrowseImage> name=NC_000913:3958700..3960721 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 3922479..3924500
<gbrowseImage> name=NC_012967:3922479..3924500 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 3848369..3850390
<gbrowseImage> name=NC_012759:3848369..3850390 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 3676004..3673983
<gbrowseImage> name=AC_000091:3673983..3676004 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 4057620..4059641
<gbrowseImage> name=NC_010473:4057620..4059641 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

3958700

Edman degradation

PMID:2999067[3]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

rep(del) (Keio:JW5604)

deletion

deletion

PMID:16738554[4]

Shigen

rep::Tn5KAN-I-SceI (FB21451)

Insertion at nt 508 in Minus orientation

PMID:15262929[5]

E. coli Genome Project:FB21451

contains pKD46

Δrep::kan

deletion

Biolog:respiration

unable to respire Acetate

PMID:16095938[6]

Δrep::kan

deletion

Biolog:respiration

unable to respire a-Hydroxybutyrate

PMID:16095938[6]

Δrep::kan

deletion

Biolog:respiration

unable to respire a-Ketobutyrate

PMID:16095938[6]

Δrep::kan

deletion

Biolog:respiration

unable to respire a-Ketoglutarate

PMID:16095938[6]

Δrep::kan

deletion

Biolog:respiration

unable to respire Succinate

PMID:16095938[6]

Δrep::kan

deletion

Biolog:respiration

unable to respire Bromosuccinate

PMID:16095938[6]

Δrep::kan

deletion

Biolog:respiration

unable to respire L-Alanine

PMID:16095938[6]

Δrep::kan

deletion

Biolog:respiration

unable to respire L-Asparagine

PMID:16095938[6]

Δrep::kan

deletion

Biolog:respiration

unable to respire L-Aspartate

PMID:16095938[6]

Δrep::kan

deletion

Biolog:respiration

unable to respire L-Serine

PMID:16095938[6]

rep-5

CGSC:7013

rep-3

PMID:383240[7]

CGSC:9794

rep-142(ts)

temperature sensitive

CGSC:90737

[rep-71]

PMID:6286604[8]

CGSC:90823

null allele

rep-729(del)::kan

PMID:16738554[4]

CGSC:113537

rep in MDS42

deletion

Sensitivity to

increased sensitivity to Bicyclomycin

PMID:21183718[9]

Fig. 2

mbrA4

Growth Phenotype

no growth in the first few hours at non-permissive conditions but eventually resumed growth, after exposed to LB cells began to elongate LB-Mac-

PMID:2176633[10]

mbrA15

Growth Phenotype

temperature sensitive, forms two distinct populations, one more dense and one less dense than wild type

PMID:2176633[10]

mbrA4

Resistant to

camphor resistance

PMID:2176633[10]

PMID:1925019[11]

Table 2 and table 1

mrbA15

Resistant to

Resistant to camphor

PMID:2176633[10]

PMID:1925019[11]

table 2 and table 1

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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW5604

Plasmid clone

Shigen

PMID:16769691[12]

Status:cloned as old JW

Primer 1:GCCCGTCTAAACCCCGGCCAACA

Primer 2:CCTTTCCCTCGTTTTGCCGCCAT

2A8

Kohara Phage

Genobase

PMID:3038334[13]

2E6

Kohara Phage

Genobase

PMID:3038334[13]

ilvD500::Tn10

Linked marker

CAG18431 = CGSC7462[14]

est. P1 cotransduction: 83% [15]
Synonyms:ilv500::Tn10

metEo-3079::Tn10

Linked marker

CAG18491 = CGSC7464[14]

est. P1 cotransduction: 10% [15]
Synonyms:metE3079::Tn10 nnnThe Tn10 in CAG13491 is inserted in the metE regulatory region, but causes a Met- phenotype.

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10837

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10837

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:948292

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000828

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0830

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0012344

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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<protect>

References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ Bialkowska-Hobrzanska, H et al. (1985) Escherichia coli rep gene: identification of the promoter and N terminus of the rep protein. J. Bacteriol. 164 1004-10 PubMed
  4. ↑ 4.0 4.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  5. ↑ Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
  6. ↑ 6.00 6.01 6.02 6.03 6.04 6.05 6.06 6.07 6.08 6.09 Ito, M et al. (2005) Functional analysis of 1440 Escherichia coli genes using the combination of knock-out library and phenotype microarrays. Metab. Eng. 7 318-27 PubMed
  7. ↑ Takahashi, S et al. (1979) The rep mutation. VI. Purification and properties of the Escherichia coli rep protein, DNA helicase III. Can. J. Biochem. 57 855-66 PubMed
  8. ↑ Tessman, I et al. (1982) Relative map location of the rep and rho genes of Escherichia coli. J. Bacteriol. 151 1637-40 PubMed
  9. ↑ Washburn, RS & Gottesman, ME (2011) Transcription termination maintains chromosome integrity. Proc. Natl. Acad. Sci. U.S.A. 108 792-7 PubMed
  10. ↑ 10.0 10.1 10.2 10.3 Trun, NJ & Gottesman, S (1990) On the bacterial cell cycle: Escherichia coli mutants with altered ploidy. Genes Dev. 4 2036-47 PubMed
  11. ↑ 11.0 11.1 Trun, NJ & Gottesman, S () Characterization of Escherichia coli mutants with altered ploidy. Res. Microbiol. 142 195-200 PubMed
  12. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  13. ↑ 13.0 13.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  14. ↑ 14.0 14.1 CGSC: The Coli Genetics Stock Center
  15. ↑ 15.0 15.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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