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Revision as of 16:39, 17 June 2010

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

crp

Mnemonic

cAMP receptor protein

Synonyms

ECK3345, b3357, JW5702, cap, csm, gurB, G51[1][2]

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

75.09 minutes, 75.09 minutes 

MG1655: 3484142..3484774
gbrowse_img.php?coord=3484142..3484774&genome=ecoli&name=MG1655&trackset=0&width=300&.png

REL606

NC_012967: 3414025..3414657
gbrowse_img.php?coord=3414025..3414657&genome=REL606&name=NC_012967&trackset=0&width=300&.png

BW2952

NC_012759: 3371299..3371931
gbrowse_img.php?coord=3371299..3371931&genome=BW2952&name=NC_012759&trackset=0&width=300&.png

W3110

 

W3110: 4154296..4153664
gbrowse_img.php?coord=4153664..4154296&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

DH10B

DH10B: 3581887..3582519
gbrowse_img.php?coord=3581887..3582519&genome=ecoli_DH10B&name=DH10B&trackset=0&width=300&.png

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

3484145

Edman degradation

PMID:6271171[3]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

crpR181L

R181L

Suppresses DNA-binding

seeded from UniProt:P0ACJ8

crpR186K

R186K

No modification of DNA-binding

seeded from UniProt:P0ACJ8

crpR186L

R186L

Marginally reduced DNA-binding

seeded from UniProt:P0ACJ8

crpS129A

S129A

Reduced DNA-binding; no modification of cAMP-binding

seeded from UniProt:P0ACJ8

crpR181K

R181K

Suppresses DNA-binding

seeded from UniProt:P0ACJ8

crpS63A

S63A

Enhanced cAMP-binding

seeded from UniProt:P0ACJ8

crpR83L

R83L

Loss of cAMP-binding

seeded from UniProt:P0ACJ8

crpS84A,K

S84A,K

No modification of cAMP-binding

seeded from UniProt:P0ACJ8

crpT128A

T128A

No modification of cAMP-binding

seeded from UniProt:P0ACJ8

Δcrp (Keio:JW5702)

deletion

deletion

PMID:16738554[4]

Shigen

crp::Tn5KAN

PMID:12519969[5]

ASAP

At position 369 in Minus orientation, contains plasmid pKD46.
ASAP strain name: FB21127

crp::Tn5KAN

PMID:12519969[5]

ASAP

At position 369 in Minus orientation, does not contain plasmid pKD46.
ASAP strain name: FB21128

Δcrp::kan

deletion

Biolog:respiration

unable to respire Dextrin

PMID:16095938[6]

Δcrp::kan

deletion

Biolog:respiration

unable to respire N-Acetyl-D-glucosamine

PMID:16095938[6]

Δcrp::kan

deletion

Biolog:respiration

unable to respire L-Fucose

PMID:16095938[6]

Δcrp::kan

deletion

Biolog:respiration

unable to respire D-Galactose

PMID:16095938[6]

Δcrp::kan

deletion

Biolog:respiration

unable to respire Maltose

PMID:16095938[6]

Δcrp::kan

deletion

Biolog:respiration

unable to respire D-Sorbitol

PMID:16095938[6]

Δcrp::kan

deletion

Biolog:respiration

unable to respire Glycerol

PMID:16095938[6]

Δcrp::kan

deletion

Biolog:respiration

unable to respire DL-a-Glycerol

PMID:16095938[6]

Δcrp::kan

deletion

Biolog:respiration

unable to respire D-Galactoniate

PMID:16095938[6]

Δcrp::kan

deletion

Biolog:respiration

unable to respire D-Galacturonate

PMID:16095938[6]

Δcrp::kan

deletion

Biolog:respiration

unable to respire D-Gluconate

PMID:16095938[6]

Δcrp::kan

deletion

Biolog:respiration

unable to respire D-Glucuronate

PMID:16095938[6]

Δcrp::kan

deletion

Biolog:respiration

unable to respire Acetate

PMID:16095938[6]

Δcrp::kan

deletion

Biolog:respiration

unable to respire a-Hydroxybutyrate

PMID:16095938[6]

Δcrp::kan

deletion

Biolog:respiration

unable to respire a-Ketobutyrate

PMID:16095938[6]

Δcrp::kan

deletion

Biolog:respiration

unable to respire a-Ketoglutarate

PMID:16095938[6]

Δcrp::kan

deletion

Biolog:respiration

unable to respire DL-Lactate

PMID:16095938[6]

Δcrp::kan

deletion

Biolog:respiration

unable to respire Succinate

PMID:16095938[6]

Δcrp::kan

deletion

Biolog:respiration

unable to respire Bromosuccinate

PMID:16095938[6]

Δcrp::kan

deletion

Biolog:respiration

unable to respire Glucuronamide

PMID:16095938[6]

Δcrp::kan

deletion

Biolog:respiration

unable to respire L-Alanine

PMID:16095938[6]

Δcrp::kan

deletion

Biolog:respiration

unable to respire L-Asparagine

PMID:16095938[6]

Δcrp::kan

deletion

Biolog:respiration

unable to respire L-Aspartate

PMID:16095938[6]

Δcrp::kan

deletion

Biolog:respiration

unable to respire Glycyl-L-aspartate

PMID:16095938[6]

Δcrp::kan

deletion

Biolog:respiration

unable to respire D-Serine

PMID:16095938[6]

Δcrp::kan

deletion

Biolog:respiration

unable to respire L-Serine

PMID:16095938[6]

Δcrp-3

CGSC:4523

Δcrp-45

CGSC:7790

crp-1

CGSC:7936

crp-2

CGSC:14257

crp-1004

CGSC:17279

Δcrp-39

CGSC:17281

Δcrp-765::kan

PMID:16738554[4]

CGSC:113694


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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW5702

Plasmid clone

Shigen

PMID:16769691[7]

Status:cloned as old JW

Primer 1:GCCGTGCTTGGCAAACCGCAAAC

Primer 2:CCACGAGTGCCGTAAACGACGAT

smg-3082::Tn10

Linked marker

CAG12071 = CGSC7444[8]

est. P1 cotransduction: 7% [9]
Synonyms:zhb-3082::Tn10, zhd-3082::Tn10

zhf-3084::Tn10

Linked marker

CAG18456 = CGSC7576[8]

est. P1 cotransduction: 51% [9]
Synonyms:zhe-3084::Tn10nnnCAG18456 also carries cysG0 (CGSC).

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10164

EchoBASE (EcoliWiki Page)

EchoBASE:EB0162

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b3357

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C4132

EcoGene (EcoliWiki Page)

EcoGene:EG10164

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:CRP

CGSC (EcoliWiki Page)

CGSC:906

ASAP (EcoliWikiPage)

ASAP:ABE-0010970


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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ Aiba, H & Krakow, JS (1981) Isolation and characterization of the amino and carboxyl proximal fragments of the adenosine cyclic 3' ,5'-phosphate receptor protein of Escherichia coli. Biochemistry 20 4774-80 PubMed
  4. ↑ 4.0 4.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  5. ↑ 5.0 5.1 Glasner, JD et al. (2003) ASAP, a systematic annotation package for community analysis of genomes. Nucleic Acids Res. 31 147-51 PubMed
  6. ↑ 6.00 6.01 6.02 6.03 6.04 6.05 6.06 6.07 6.08 6.09 6.10 6.11 6.12 6.13 6.14 6.15 6.16 6.17 6.18 6.19 6.20 6.21 6.22 6.23 6.24 6.25 Ito, M et al. (2005) Functional analysis of 1440 Escherichia coli genes using the combination of knock-out library and phenotype microarrays. Metab. Eng. 7 318-27 PubMed
  7. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  8. ↑ 8.0 8.1 CGSC: The Coli Genetics Stock Center
  9. ↑ 9.0 9.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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