recA:Gene: Difference between revisions
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!|Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links | !|Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links | ||
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W3110: 2822425..2821364<br /><gbrowseImage> | W3110: 2822425..2821364<br /><gbrowseImage> | ||
name= | name=NC_007779:2821364..2822425 | ||
source=W3110 | source=W3110 | ||
preset=GeneLocation | preset=GeneLocation | ||
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{{GBrowseLink|W3110| | {{GBrowseLink|W3110|NC_007779|2820364|2823425|GBrowse(W3110)}} | ||
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{{GBrowseFastaLink|W3110| | {{GBrowseFastaLink|W3110|NC_007779|2821364|2822425|FASTA Dump}} | ||
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!|Feature Type!!Strain!!Genomic Location!!Evidence!!Reference!!Notes | !|Feature Type!!Strain!!Genomic Location!!Evidence!!Reference!!Notes | ||
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{| | {| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="Z46a9e414d7fcb" class=" tableEdit Gene_allele_table dataTable" | ||
|- {{table_heading_style}} | |- {{table_heading_style}} | ||
!|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments | !|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments | ||
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''recA(del)'' (Keio:JW2669) | |||
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deletion | deletion | ||
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PMID:16738554 | PMID:16738554 | ||
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[http://www.shigen.nig.ac.jp/ecoli/strain/keioCollectionQueryAction.do Shigen]<br>[http://cgsc2.biology.yale.edu/Strain.php?ID=108386 CGSC10105]<ref name='LIB:cgsc'/> | [http://www.shigen.nig.ac.jp/ecoli/strain/keioCollectionQueryAction.do Shigen]<br />[http://cgsc2.biology.yale.edu/Strain.php?ID=108386 CGSC10105]<ref name='LIB:cgsc'/> | ||
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''recA(del)''::kan | |||
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deletion | deletion-replacement | ||
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''recA(del)::kan'' | |||
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deletion | deletion | ||
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''recA(del)::kan'' | |||
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deletion | deletion | ||
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'' | ''recA635(del)::kan'' | ||
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'' | ''recA636''(del-ins)''::FRT'' | ||
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'' | ''recA774(del)::kan'' | ||
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recA | ''recA'' | ||
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deletion | deletion | ||
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fig 2 | fig 2 | ||
|- | |||
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''recA(del)'' | |||
| | |||
deletion | |||
| | |||
| | |||
Growth Phenotype | |||
| | |||
When the ''recA'' gene was deleted there was only a marginal change in the growth rate compared to the wild type when exposed to the same concentration of levofloxacin. | |||
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PMID:20660686 | |||
| | |||
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See figure 1. | |||
|- | |||
| | |||
''recA(del)'' | |||
| | |||
The ''recA'' was deleted. | |||
| | |||
| | |||
Resistant to | |||
| | |||
After the ''recA'' gene was deleted there was only a marginal difference in the level of resistance compared to the wild type strain when exposed to the same concentration of levofloxacin. | |||
| | |||
PMID:20660686 | |||
| | |||
| | |||
Figure 1B. | |||
|- | |||
| | |||
''recA(del)'' | |||
| | |||
The ''recA'' gene was deleted. | |||
| | |||
| | |||
Resistant to | |||
| | |||
The number of ''recA(del)'' levofloxacin resistant mutants was fewer than the wildtype and did not appear as quickly. | |||
| | |||
PMID:20660686 | |||
| | |||
| | |||
See Figure 4. | |||
|- | |||
| | |||
''recA(del)'' | |||
| | |||
The ''recA'' gene was deactivated. | |||
| | |||
| | |||
Sensitivity to | |||
| | |||
Mutant frequency decreased with the introduction of sublethal dosages of antibiotics. | |||
| | |||
PMID:21212055 | |||
| | |||
| | |||
See Figure 1 | |||
|- | |||
| | |||
''recA(del)'' | |||
| | |||
The recA gene was deactivated. | |||
| | |||
| | |||
Rate transcription | |||
| | |||
the rate of transcription of ''recA::GFP'' was increased with the additon antimicrobials | |||
| | |||
PMID:21212055 | |||
| | |||
| | |||
See figure 2 | |||
|- | |||
| | |||
''recA(del)'' | |||
| | |||
The ''recA'' gene was deactivated. | |||
| | |||
| | |||
Growth Phenotype | |||
| | |||
The addition of the antimicrobials ciprofloxacin, trimethoprim and sulfamethoxazole to the ''recA'' deficient mutant caused the formation of reduced filaments. | |||
| | |||
PMID:21212055 | |||
| | |||
| | |||
See figure 3(b) | |||
|- | |||
| | |||
''nrdA101recA(del)'' | |||
| | |||
'recA'' is deactivated. | |||
| | |||
| | |||
rate of replication | |||
| | |||
Replication is stalled when the replication forks are reversed due to the absence of ''recA'' | |||
| | |||
PMID:21441507 | |||
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| | |||
|- | |||
| | |||
''recA(del)'' | |||
| | |||
the ''recA'' gene was deactiaved. | |||
| | |||
| | |||
Sensitivity to | |||
| | |||
By deactivating the ''recA'' gene there is an increase in sensitvity to UV radiation. | |||
| | |||
PMID:2112525 | |||
| | |||
| | |||
See Figure 2 A. | |||
|- | |||
| | |||
''recA(del)'' | |||
| | |||
the ''recA'' gene was deactiaved. | |||
| | |||
| | |||
Rate of Recombination | |||
| | |||
By deactivating the ''recA'' gene there is a decrease in the rate of recombination frequency compared to the wildtype strain. | |||
| | |||
PMID:2112525 | |||
| | |||
| | |||
See Figure 2 B. | |||
|- | |||
| | |||
''recA(del)'' | |||
| | |||
the ''recA'' gene was deactiaved. | |||
| | |||
| | |||
rate of ''LexA'' cleavage | |||
| | |||
By deactivating the ''recA'' gene there is a decrease ''LexA'' cleavage when compared to the wildtype. | |||
| | |||
PMID:2112525 | |||
| | |||
| | |||
See Figure 2 C. | |||
|- | |||
| | |||
''recA142'' | |||
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| | |||
| | |||
Sensitivity to | |||
| | |||
mutants are more sensitive to DNA damaging agent Mitomycin C, fig 2. | |||
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PMID:17040104 | |||
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|- | |||
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''redA'' in strain F19 | |||
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Resistant to | |||
| | |||
Resistant to Muteonidazole resistance | |||
| | |||
PMID:1991710 | |||
| | |||
Strain: F19 | |||
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Figure 1 | |||
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!|Interactor!!Interaction!!Allele!!Score(s)!!Reference(s)!!Accessions!!Notes | !|Interactor!!Interaction!!Allele!!Score(s)!!Reference(s)!!Accessions!!Notes | ||
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!|Database!!Accession!!Notes | !|Database!!Accession!!Notes | ||
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[[EcoCyc]] | |||
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EcoCyc:EG10823 | |||
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[ | ''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]] | ||
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Escherichia coli str. K-12 substr. MG1655 | [[EcoGene]] | ||
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EcoGene:EG10823 | |||
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]] | |||
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[[RegulonDB]] | |||
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RegulonDB:ECK120000814 | |||
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Escherichia coli str. K-12 substr. MG1655 | ''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]] | ||
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Escherichia coli str. K-12 substr. MG1655 | Escherichia coli str. K-12 substr. MG1655 | ||
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[[EchoBASE]] | |||
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EchoBASE:EB0816 | |||
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]] | |||
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[[ASAP]] | |||
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ASAP:ABE-0008876 | |||
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]] | |||
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!|Name!!URL!!Comments | !|Name!!URL!!Comments | ||
Latest revision as of 20:43, 19 September 2013
{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>
| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Location(s) and DNA Sequence | Sequence Features | Alleles and Phenotypes | Genetic Interactions | Genetic Resources | Accessions | Links | References | Suggestions |
Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
| Standard name |
recA |
|---|---|
| Mnemonic |
Recombination |
| Synonyms |
ECK2694, b2699, JW2669, lexB, srf, umuB, zab, recH, rnmB, tif, umuR[1], umuR |
| edit table |
Notes
Location(s) and DNA Sequence
See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
|
MG1655 |
60.8 minutes |
MG1655: 2821791..2820730 |
||
|
NC_012967: 2718628..2717567 |
||||
|
NC_012759: 2706542..2707603 |
||||
|
W3110 |
|
W3110: 2822425..2821364 |
||
| edit table |
Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
| Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
|---|---|---|---|---|---|
|
Coding Start (SO:0000323) |
2820733 |
Edman degradation |
| ||
| edit table |
Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
| Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
|---|---|---|---|---|---|---|---|
|
Strain DH10B |
G161D |
Nonsynonomous mutation |
This is a SNP in E. coli K-12 Strain DH10B | ||||
|
recA(del) (Keio:JW2669) |
deletion |
deletion |
|||||
|
recA::Tn5KAN-I-SceI (FB20884) |
Insertion at nt 407 in Plus orientation |
does not contain pKD46 | |||||
|
recA::Tn5KAN-I-SceI (FB20885) |
Insertion at nt 407 in Plus orientation |
contains pKD46 | |||||
|
recA(del)::kan |
deletion-replacement |
Biolog:respiration |
unable to respire a-Ketoglutarate |
||||
|
recA(del)::kan |
deletion |
Biolog:respiration |
unable to respire Succinate |
||||
|
recA(del)::kan |
deletion |
Biolog:respiration |
unable to respire L-Asparagine |
||||
|
recA938::Tn9-200 |
|||||||
|
recA1 |
|||||||
|
recA56 |
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|
recA13 |
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|
recA44(ts) |
temperature sensitive |
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|
recA41 |
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|
recA441(ts) |
temperature sensitive |
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|
recA430 |
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|
recA3 |
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|
recA11 |
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|
recA200(ts) |
temperature sensitive |
||||||
|
recA14 |
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|
recA12 |
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|
recA431 |
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|
recA34 |
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|
recA99(Am) |
amber (UAG) mutation | ||||||
|
recA142 |
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|
recA111 |
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|
recA629 |
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|
recA2111::RP4-2Tc::Mu |
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|
recA635(del)::kan |
|||||||
|
recA636(del-ins)::FRT |
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|
recA2 |
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|
recA1907::cat-aadA |
|||||||
|
recA774(del)::kan |
|||||||
|
recA730 |
constitutively activated form of RecA so it cleaves the LexA repressor in the absence of SOS induction |
||||||
|
recA |
deletion |
Sensitivity to |
increased sensitivity to Bicyclomycin |
fig 2 | |||
|
recA(del) |
deletion |
Growth Phenotype |
When the recA gene was deleted there was only a marginal change in the growth rate compared to the wild type when exposed to the same concentration of levofloxacin. |
See figure 1. | |||
|
recA(del) |
The recA was deleted. |
Resistant to |
After the recA gene was deleted there was only a marginal difference in the level of resistance compared to the wild type strain when exposed to the same concentration of levofloxacin. |
Figure 1B. | |||
|
recA(del) |
The recA gene was deleted. |
Resistant to |
The number of recA(del) levofloxacin resistant mutants was fewer than the wildtype and did not appear as quickly. |
See Figure 4. | |||
|
recA(del) |
The recA gene was deactivated. |
Sensitivity to |
Mutant frequency decreased with the introduction of sublethal dosages of antibiotics. |
See Figure 1 | |||
|
recA(del) |
The recA gene was deactivated. |
Rate transcription |
the rate of transcription of recA::GFP was increased with the additon antimicrobials |
See figure 2 | |||
|
recA(del) |
The recA gene was deactivated. |
Growth Phenotype |
The addition of the antimicrobials ciprofloxacin, trimethoprim and sulfamethoxazole to the recA deficient mutant caused the formation of reduced filaments. |
See figure 3(b) | |||
|
nrdA101recA(del) |
'recA is deactivated. |
rate of replication |
Replication is stalled when the replication forks are reversed due to the absence of recA |
||||
|
recA(del) |
the recA gene was deactiaved. |
Sensitivity to |
By deactivating the recA gene there is an increase in sensitvity to UV radiation. |
See Figure 2 A. | |||
|
recA(del) |
the recA gene was deactiaved. |
Rate of Recombination |
By deactivating the recA gene there is a decrease in the rate of recombination frequency compared to the wildtype strain. |
See Figure 2 B. | |||
|
recA(del) |
the recA gene was deactiaved. |
rate of LexA cleavage |
By deactivating the recA gene there is a decrease LexA cleavage when compared to the wildtype. |
See Figure 2 C. | |||
|
recA142 |
Sensitivity to |
mutants are more sensitive to DNA damaging agent Mitomycin C, fig 2. |
|||||
|
redA in strain F19 |
Resistant to |
Resistant to Muteonidazole resistance |
Strain: F19 |
Figure 1 | |||
| edit table |
Notes
Genetic Interactions
| Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
|---|---|---|---|---|---|---|
| edit table |
Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
|
JW2669 |
Plasmid clone |
Status:Clone OK Primer 1:GCCGCTATCGACGAAAACAAACA Primer 2:CCAAAATCTTCGTTAGTTTCTGC | |
|
Kohara Phage |
|||
|
Linked marker |
est. P1 cotransduction: % [29] | ||
|
Linked marker |
est. P1 cotransduction: 85% [29] | ||
| edit table |
Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
| edit table |
Notes
Links
| Name | URL | Comments |
|---|---|---|
| edit table |
<protect>
References
See Help:References for how to manage references in EcoliWiki.
- ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ Horii, T et al. (1980) Organization of the recA gene of Escherichia coli. Proc. Natl. Acad. Sci. U.S.A. 77 313-7 PubMed
- ↑ Sancar, A et al. (1980) Sequences of the recA gene and protein. Proc. Natl. Acad. Sci. U.S.A. 77 2611-5 PubMed
- ↑ Durfee, T et al. (2008) The complete genome sequence of Escherichia coli DH10B: insights into the biology of a laboratory workhorse. J. Bacteriol. 190 2597-606 PubMed
- ↑ 5.0 5.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
- ↑ 6.0 6.1 6.2 CGSC: The Coli Genetics Stock Center
- ↑ 7.0 7.1 Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
- ↑ 8.0 8.1 8.2 Ito, M et al. (2005) Functional analysis of 1440 Escherichia coli genes using the combination of knock-out library and phenotype microarrays. Metab. Eng. 7 318-27 PubMed
- ↑ 9.0 9.1 9.2 Kawashima, H et al. (1984) Functional domains of Escherichia coli recA protein deduced from the mutational sites in the gene. Mol. Gen. Genet. 193 288-92 PubMed
- ↑ 10.0 10.1 10.2 10.3 10.4 Templin, A et al. (1978) Suppressibility of recA, recB, and recC mutations by nonsense suppressors. J. Bacteriol. 134 590-6 PubMed
- ↑ 11.0 11.1 11.2 11.3 11.4 11.5 Dutreix, M et al. (1989) New recA mutations that dissociate the various RecA protein activities in Escherichia coli provide evidence for an additional role for RecA protein in UV mutagenesis. J. Bacteriol. 171 2415-23 PubMed
- ↑ Lavery, PE & Kowalczykowski, SC (1990) Properties of recA441 protein-catalyzed DNA strand exchange can be attributed to an enhanced ability to compete with SSB protein. J. Biol. Chem. 265 4004-10 PubMed
- ↑ Larminat, F et al. (1992) New mutations in and around the L2 disordered loop of the RecA protein modulate recombination and/or coprotease activity. J. Bacteriol. 174 6264-9 PubMed
- ↑ 14.0 14.1 Zaitsev, E et al. (1994) Nucleotide sequence between recA and alaSp in E. coli K12 and the sequence change in four recA mutations. Mutat. Res. 323 173-7 PubMed
- ↑ Fuerst, CR & Siminovitch, L (1965) Characterization of an unusual defective lysogenic strain of Escherichia coli K-12(lambda). Virology 27 449-51 PubMed
- ↑ Knight, KL et al. (1984) Identification of the amino acid substitutions in two mutant forms of the recA protein from Escherichia coli: recA441 and recA629. J. Biol. Chem. 259 11279-83 PubMed
- ↑ 17.0 17.1 Datsenko, KA & Wanner, BL (2000) One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc. Natl. Acad. Sci. U.S.A. 97 6640-5 PubMed
- ↑ Wanner, BL & Boline, JA (1990) Mapping and molecular cloning of the phn (psiD) locus for phosphonate utilization in Escherichia coli. J. Bacteriol. 172 1186-96 PubMed
- ↑ Lin, LL & Little, JW (1988) Isolation and characterization of noncleavable (Ind-) mutants of the LexA repressor of Escherichia coli K-12. J. Bacteriol. 170 2163-73 PubMed
- ↑ Tran, L et al. (2011) Single-gene deletion mutants of Escherichia coli with altered sensitivity to bicyclomycin, an inhibitor of transcription termination factor Rho. J. Bacteriol. 193 2229-35 PubMed
- ↑ 21.0 21.1 21.2 Singh, R et al. (2010) Impact of recA on levofloxacin exposure-related resistance development. Antimicrob. Agents Chemother. 54 4262-8 PubMed
- ↑ 22.0 22.1 22.2 Thi, TD et al. (2011) Effect of recA inactivation on mutagenesis of Escherichia coli exposed to sublethal concentrations of antimicrobials. J. Antimicrob. Chemother. 66 531-8 PubMed
- ↑ Salguero, I et al. (2011) RecA-dependent replication in the nrdA101(Ts) mutant of Escherichia coli under restrictive conditions. J. Bacteriol. 193 2851-60 PubMed
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- ↑ 29.0 29.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).