PMID:2112525
| Citation |
Harada, F, Hirose, Y, Tanaka, M, Sasaki, T, Kamiya, H and Miura, K (1990) A simple, general method for detecting retroviral RNAs expressed in cells. Jpn. J. Cancer Res. 81:232-7 |
|---|---|
| Abstract |
A simple, efficient method has been developed for detecting retroviral RNAs expressed in cells. In this method, total RNAs or poly A+ RNAs extracted from various human cells are separated by electrophoresis and hybridized with synthetic oligonucleotides corresponding to the 3'-terminal 18 nucleotides of various tRNAs. Genomic and subgenomic RNAs of HTLV-I and HTLV-II in virus-infected cells and of xenotropic murine leukemia virus expressed in human lung cancer cells were easily detected with the tRNA(Pro)-derived oligonucleotide probe. This technique can be used to search for unidentified retroviruses expressed in human cancer cells and tissues. |
| Links | |
| Keywords |
Animals; B-Lymphocytes/microbiology; Base Sequence; Cloning, Molecular; HeLa Cells; Human T-lymphotropic virus 1/genetics; Human T-lymphotropic virus 2/genetics; Mice; Mice, Inbred BALB C; Molecular Sequence Data; Neoplasm Transplantation; Nucleic Acid Hybridization; Oligonucleotide Probes; RNA, Transfer; RNA, Transfer, Pro; RNA, Viral/analysis; Repetitive Sequences, Nucleic Acid; Retroviridae/genetics; T-Lymphocytes/microbiology; Tumor Cells, Cultured |
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