recA:On One Page

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Quickview | Gene | Product(s) | Expression| Evolution | References |

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Standard Name

recA

Gene Synonym(s)

ECK2694, b2699, JW2669, lexB, srf, umuB, zab, recH, rnmB, tif, umuR[1], umuR

Product Desc.

DNA strand exchange and renaturation, DNA-dependent ATPase, DNA- and ATP-dependent coprotease[2]; DNA strand exchange and recombination protein with protease and nuclease activity[3]

General recombination and DNA repair; pairing and strand exchange; role in cleavage of LexA repressor, SOS mutagenesis[4]

Product Synonyms(s)

B2699[2][1], Tif[2][1], UmuB[2][1], Zab[2][1], Srf[2][1], RnmB[2][1], RecH[2][1], LexB[2][1], RecA[2][1] , ECK2694, JW2669, lexB, recH, rnmB, srf, tif, umuB, umuR, zab, b2699

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): recAX[2], recA[2], OP00086

Regulation/Activity
Quick Links

porteco.png EcoCyc.gif regulondb.jpg

dnadisplay.png proteindisplay.png   pubmed.jpg   textpresso.jpg  

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Notes

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RecA does not bind to DNA with extensive stem-loop structures, except in the presence of SSB. RecA is required for polV lesion bypass. RecA filamentation is inhibited by RecX. Binds TrxA (Kumar, 2004).[4]

RecA lacking mutants exhibited the same frequency as the wild type when exposed to sublethal concentrations of the antibiotics Ampicillin, Ceftazidime, Imipenem, Fosfomycin, Ciprofloxacin, Trimethoprim, and Sulfamethoxazole.(PMID:21212055)

RecA lacking mutants exhibited different frequencies then the wild type when exposed to sublethal concentrations of the antibiotics Gentamicin, Rifampicin, Chloramphenicol (PMID:21212055)



Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

recA

Mnemonic

Recombination

Synonyms

ECK2694, b2699, JW2669, lexB, srf, umuB, zab, recH, rnmB, tif, umuR[1], umuR

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

60.8 minutes 

MG1655: 2821791..2820730
<gbrowseImage> name=NC_000913:2820730..2821791 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 2718628..2717567
<gbrowseImage> name=NC_012967:2717567..2718628 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 2706542..2707603
<gbrowseImage> name=NC_012759:2706542..2707603 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 2822425..2821364
<gbrowseImage> name=NC_007779:2821364..2822425 source=W3110 preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

2820733

Edman degradation

PMID:6244554
PMID:6930655


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

Strain DH10B

G161D

Nonsynonomous mutation

PMID:18245285

ASAP

This is a SNP in E. coli K-12 Strain DH10B

recA(del) (Keio:JW2669)

deletion

deletion

PMID:16738554

Shigen
CGSC10105[5]

recA::Tn5KAN-I-SceI (FB20884)

Insertion at nt 407 in Plus orientation

PMID:15262929

E. coli Genome Project:FB20884

does not contain pKD46

recA::Tn5KAN-I-SceI (FB20885)

Insertion at nt 407 in Plus orientation

PMID:15262929

E. coli Genome Project:FB20885

contains pKD46

recA(del)::kan

deletion-replacement

Biolog:respiration

unable to respire a-Ketoglutarate

PMID:16095938

recA(del)::kan

deletion

Biolog:respiration

unable to respire Succinate

PMID:16095938

recA(del)::kan

deletion

Biolog:respiration

unable to respire L-Asparagine

PMID:16095938

recA938::Tn9-200

CGSC:4556

recA1

PMID:6363880 PMID:350827

CGSC:4691

recA56

PMID:2651400

CGSC:4795

recA13

PMID:2651400

CGSC:4897

recA44(ts)

temperature sensitive

PMID:2651400

CGSC:5323

recA41

CGSC:5549

recA441(ts)

temperature sensitive

PMID:6363880 PMID:2406267 PMID:350827

CGSC:6585

recA430

PMID:6363880 PMID:2651400 PMID:1400177

CGSC:6772

recA3

CGSC:6961

recA11

PMID:7512687 PMID:5321955 PMID:350827

CGSC:7623

recA200(ts)

temperature sensitive

CGSC:8413

recA14

CGSC:9230

recA12

PMID:7512687 PMID:350827

CGSC:9257

recA431

CGSC:9898

recA34

CGSC:9900

recA99(Am)

PMID:350827 PMID:2651400

CGSC:10336

amber (UAG) mutation

recA142

PMID:2651400

CGSC:11217

recA111

CGSC:14572

recA629

PMID:6088537

CGSC:19643

recA2111::RP4-2Tc::Mu

CGSC:60624

recA635(del)::kan

PMID:10829079

CGSC:64884

recA636(del-ins)::FRT

PMID:10829079

CGSC:65682

recA2

CGSC:72687

recA1907::cat-aadA

PMID:2155195

CGSC:99541

recA774(del)::kan

PMID:16738554

CGSC:102620

recA730

constitutively activated form of RecA so it cleaves the LexA repressor in the absence of SOS induction

PMID:2834329

recA

deletion

Sensitivity to

increased sensitivity to Bicyclomycin

PMID:21357484

fig 2

recA(del)

deletion

Growth Phenotype

When the recA gene was deleted there was only a marginal change in the growth rate compared to the wild type when exposed to the same concentration of levofloxacin.

PMID:20660686

See figure 1.

recA(del)

The recA was deleted.

Resistant to

After the recA gene was deleted there was only a marginal difference in the level of resistance compared to the wild type strain when exposed to the same concentration of levofloxacin.

PMID:20660686

Figure 1B.

recA(del)

The recA gene was deleted.

Resistant to

The number of recA(del) levofloxacin resistant mutants was fewer than the wildtype and did not appear as quickly.

PMID:20660686

See Figure 4.

recA(del)

The recA gene was deactivated.

Sensitivity to

Mutant frequency decreased with the introduction of sublethal dosages of antibiotics.

PMID:21212055

See Figure 1

recA(del)

The recA gene was deactivated.

Rate transcription

the rate of transcription of recA::GFP was increased with the additon antimicrobials

PMID:21212055

See figure 2

recA(del)

The recA gene was deactivated.

Growth Phenotype

The addition of the antimicrobials ciprofloxacin, trimethoprim and sulfamethoxazole to the recA deficient mutant caused the formation of reduced filaments.

PMID:21212055

See figure 3(b)

nrdA101recA(del)

'recA is deactivated.

rate of replication

Replication is stalled when the replication forks are reversed due to the absence of recA

PMID:21441507

recA(del)

the recA gene was deactiaved.

Sensitivity to

By deactivating the recA gene there is an increase in sensitvity to UV radiation.

PMID:2112525

See Figure 2 A.

recA(del)

the recA gene was deactiaved.

Rate of Recombination

By deactivating the recA gene there is a decrease in the rate of recombination frequency compared to the wildtype strain.

PMID:2112525

See Figure 2 B.

recA(del)

the recA gene was deactiaved.

rate of LexA cleavage

By deactivating the recA gene there is a decrease LexA cleavage when compared to the wildtype.

PMID:2112525

See Figure 2 C.

recA142

Sensitivity to

mutants are more sensitive to DNA damaging agent Mitomycin C, fig 2.

PMID:17040104

redA in strain F19

Resistant to

Resistant to Muteonidazole resistance

PMID:1991710

Strain: F19

Figure 1

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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW2669

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCGCTATCGACGAAAACAAACA

Primer 2:CCAAAATCTTCGTTAGTTTCTGC

23B7

Kohara Phage

Genobase

PMID:3038334

pheA18::Tn10

Linked marker

CAG12158 = CGSC7421[5]

est. P1 cotransduction: % [6]

srlD3131::Tn10

Linked marker

CAG18642 = CGSC7423[5]

est. P1 cotransduction: 85% [6]
Synonyms:zfh-3131::Tn10, zfi-3131::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10823

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10823

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000814

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:947170

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0816

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0008876

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

RecA

Synonyms

B2699[2][1], Tif[2][1], UmuB[2][1], Zab[2][1], Srf[2][1], RnmB[2][1], RecH[2][1], LexB[2][1], RecA[2][1] , ECK2694, JW2669, lexB, recH, rnmB, srf, tif, umuB, umuR, zab, b2699

Product description

DNA strand exchange and renaturation, DNA-dependent ATPase, DNA- and ATP-dependent coprotease[2]; DNA strand exchange and recombination protein with protease and nuclease activity[3]

General recombination and DNA repair; pairing and strand exchange; role in cleavage of LexA repressor, SOS mutagenesis[4]

EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status

GO:0003697

single-stranded DNA binding

PMID:19820696

IDA: Inferred from Direct Assay

F

CACAO annotation. Earlier reference would be preferable

complete

GO:0000166

nucleotide binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR003593

F

Seeded from EcoCyc (v14.0)

complete

GO:0000166

nucleotide binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0547

F

Seeded from EcoCyc (v14.0)

complete

GO:0003677

DNA binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001553

F

Seeded from EcoCyc (v14.0)

complete

GO:0003677

DNA binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR020584

F

Seeded from EcoCyc (v14.0)

complete

GO:0003677

DNA binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR020587

F

Seeded from EcoCyc (v14.0)

complete

GO:0003677

DNA binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR020588

F

Seeded from EcoCyc (v14.0)

complete

GO:0003677

DNA binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0238

F

Seeded from EcoCyc (v14.0)

complete

GO:0003684

damaged DNA binding

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00268

F

Seeded from EcoCyc (v14.0)

complete

GO:0003697

single-stranded DNA binding

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00268

F

Seeded from EcoCyc (v14.0)

complete

GO:0003697

single-stranded DNA binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR013765

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00268

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001553

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR013765

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR020584

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR020587

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR020588

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0067

F

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00268

C

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR013765

C

Seeded from EcoCyc (v14.0)

complete

GO:0048870

cell motility

PMID:17391508

IMP: Inferred from Mutant Phenotype

P

complete

GO:0005737

cytoplasm

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0963

C

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GO_REF:0000023

IEA: Inferred from Electronic Annotation

SP_SL:SL-0086

C

Seeded from EcoCyc (v14.0)

complete

GO:0006259

DNA metabolic process

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001553

P

Seeded from EcoCyc (v14.0)

complete

GO:0006259

DNA metabolic process

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR020584

P

Seeded from EcoCyc (v14.0)

complete

GO:0006259

DNA metabolic process

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR020587

P

Seeded from EcoCyc (v14.0)

complete

GO:0006259

DNA metabolic process

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR020588

P

Seeded from EcoCyc (v14.0)

complete

GO:0006281

DNA repair

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00268

P

Seeded from EcoCyc (v14.0)

complete

GO:0006281

DNA repair

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR013765

P

Seeded from EcoCyc (v14.0)

complete

GO:0006974

response to DNA damage stimulus

PMID:10760155

IEP: Inferred from Expression Pattern

P

complete

GO:0006281

DNA repair

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0234

P

Seeded from EcoCyc (v14.0)

complete

GO:0006310

DNA recombination

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00268

P

Seeded from EcoCyc (v14.0)

complete

GO:0006310

DNA recombination

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0233

P

Seeded from EcoCyc (v14.0)

complete

GO:0006950

response to stress

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0346

P

Seeded from EcoCyc (v14.0)

complete

GO:0006974

response to DNA damage stimulus

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00268

P

Seeded from EcoCyc (v14.0)

complete

GO:0006974

response to DNA damage stimulus

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0227

P

Seeded from EcoCyc (v14.0)

complete

GO:0008094

DNA-dependent ATPase activity

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00268

F

Seeded from EcoCyc (v14.0)

complete

GO:0008094

DNA-dependent ATPase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001553

F

Seeded from EcoCyc (v14.0)

complete

GO:0008094

DNA-dependent ATPase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR020584

F

Seeded from EcoCyc (v14.0)

complete

GO:0008094

DNA-dependent ATPase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR020587

F

Seeded from EcoCyc (v14.0)

complete

GO:0008094

DNA-dependent ATPase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR020588

F

Seeded from EcoCyc (v14.0)

complete

GO:0009432

SOS response

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00268

P

Seeded from EcoCyc (v14.0)

complete

GO:0009432

SOS response

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0742

P

Seeded from EcoCyc (v14.0)

complete

GO:0017111

nucleoside-triphosphatase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR003593

F

Seeded from EcoCyc (v14.0)

complete

GO:0006974

response to DNA damage stimulus

PMID:11967071

IEP: Inferred from Expression Pattern

P

complete

GO:0009432

SOS response

PMID:4600265

IMP: Inferred from Mutant Phenotype

P

Fig. 1 and 2

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

napC

PMID:16606699

Experiment(s):EBI-1143694

Protein

rplJ

PMID:16606699

Experiment(s):EBI-1143694

Protein

nadE

PMID:16606699

Experiment(s):EBI-1143694

Protein

rplB

PMID:16606699

Experiment(s):EBI-1143694

Protein

yfiR

PMID:16606699

Experiment(s):EBI-1143694

Protein

groS

PMID:19402753

LCMS(ID Probability):99.6

Protein

fusA

PMID:19402753

LCMS(ID Probability):99.6

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MAIDENKQKA LAAALGQIEK QFGKGSIMRL GEDRSMDVET ISTGSLSLDI ALGAGGLPMG
RIVEIYGPES SGKTTLTLQV IAAAQREGKT CAFIDAEHAL DPIYARKLGV DIDNLLCSQP
DTGEQALEIC DALARSGAVD VIVVDSVAAL TPKAEIEGEI GDSHMGLAAR MMSQAMRKLA
GNLKQSNTLL IFINQIRMKI GVMFGNPETT TGGNALKFYA SVRLDIRRIG AVKEGENVVG
SETRVKVVKN KIAAPFKQAE FQILYGEGIN FYGELVDLGV KEKLIEKAGA WYSYKGEKIG
QGKANATAWL KDNPETAKEI EKKVRELLLS NPNSTPDFSV DDSEGVAETN EDF
Length

353

Mol. Wt

37.974 kDa

pI

5.0 (calculated)

Extinction coefficient

21,430 - 21,805 (calc based on 7 Y, 2 W, and 3 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Initiator Methionine

1

Removed

UniProt:P0A7G6

Domain

9..330

PF00154 recA bacterial DNA recombination protein

PMID:19920124

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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=recA taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16130606

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:947170

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0008876

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P0A7G6

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10823

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10823

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:947170

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000814

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0816

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

Protein

E. coli K-12 MC4100

6.86E+02

molecules/cell

  • Medium: median data from rich and minimal media
  • Temperature (°C): 37
  • Doubling time (min): Growth Rate(min):
  • Growth phase: other:

emPAI

PMID:18304323

Protein

E. coli K-12 MG1655

10377

molecules/cell/generation

  • Medium: MOPS Complete
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

2926

molecules/cell/generation

  • Medium: MOPS Minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

5850

molecules/cell/generation

  • Medium: MOPS Complete without Methionine
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

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Notes

Transcription and Transcriptional Regulation

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<protect>

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

recAX

recA

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Figure courtesy of RegulonDB

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Notes

LexA-regulated[7]

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:2821771..2821811 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GEO Profiles:b2699 (EcoliWiki Page)

NCBI GEO profiles for recA

microarray

GenExpDB:b2699 (EcoliWiki Page)

Summary of data for recA from multiple microarray studies

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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes

GFP Fusion

Intergenic region (2821707..2821893) fused to gfpmut2.

OpenBioSystems

GFP fusion described in Zaslaver, et al.
Plate:109-AZ20; Well:G5[8]

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Notes

Accessions Related to recA Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10823

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0816

Escherichia coli str. K-12 substr. MG1655

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b2699

EcoGene

EcoGene:EG10823

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000814

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0008876

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism Homologs (Statistics) Comments

Arabidopsis thaliana

  • AT1G79050 (score: 1.000; bootstrap: 65%)

From Inparanoid:20070104

Caenorhabditis briggsae

  • WBGene00027081 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Caenorhabditis elegans

  • WBGene00004297 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Canis familiaris

  • ENSCAFP00000001990 (score: 1.000; bootstrap: 100%)
  • ENSCAFP00000013557 (score: 0.471)
  • ENSCAFP00000025883 (score: 0.062)

From Inparanoid:20070104

Ciona intestinalis

  • ENSCINP00000016274 (score: 1.000; bootstrap: 100%)
  • ENSCINP00000019726 (score: 0.250)

From Inparanoid:20070104

Dictyostelium discoideum

  • DDB0231571 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Homo sapiens

  • ENSP00000216024 (score: 1.000; bootstrap: 100%)
  • ENSP00000267868 (score: 0.453)
  • ENSP00000340560 (score: 0.064)
  • ENSP00000336701 (score: 0.059)

From Inparanoid:20070104

Monodelphis domestica

  • ENSMODP00000012067 (score: 1.000; bootstrap: 100%)
  • ENSMODP00000000325 (score: 0.444)
  • ENSMODP00000018294 (score: 0.057)

From Inparanoid:20070104

Mus musculus

  • MGI:105393 (score: 1.000; bootstrap: 100%)
  • MGI:97890 (score: 0.450)
  • MGI:1099436 (score: 0.061)
  • MGI:2150020 (score: 0.056)

From Inparanoid:20070104

Oryza gramene

  • Q5N6Y9 (score: 1.000; bootstrap: 97%)
  • Q7F1X7 (score: 0.653)
  • Q53QJ3 (score: 0.309)

From Inparanoid:20070104

Pan troglodytes

  • ENSPTRP00000024757 (score: 1.000; bootstrap: 100%)
  • ENSPTRP00000011825 (score: 0.453)
  • ENSPTRP00000016062 (score: 0.059)
  • ENSPTRP00000010973 (score: 0.057)

From Inparanoid:20070104

Rattus norvegicus

  • ENSRNOP00000018526 (score: 1.000; bootstrap: 100%)
  • ENSRNOP00000041615 (score: 0.230)
  • ENSRNOP00000008846 (score: 0.057)

From Inparanoid:20070104

Saccharomyces cerevisiae

  • YER095W (score: 1.000; bootstrap: 100%)
  • YER179W (score: 0.327)

From Inparanoid:20070104

Schizosaccharomyces pombe

  • SPAC6444c (score: 1.000; bootstrap: 100%)
  • SPAC8E113c (score: 0.358)

From Inparanoid:20070104

Shigella flexneri

RECA

From SHIGELLACYC

E. coli O157

RECA

From ECOO157CYC

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Do-It-Yourself Web Tools

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Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

Superfamily (EcoliWiki Page)

SUPERFAMILY:52540

Panther (EcoliWiki Page)

PTHR22942:SF1

Superfamily (EcoliWiki Page)

SUPERFAMILY:54752

Pfam (EcoliWiki Page)

PF00154 recA bacterial DNA recombination protein

EcoCyc

EcoCyc:EG10823

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10823

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000814

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0816

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0008876

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 1.15 1.16 1.17 1.18 1.19 1.20 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 2.13 2.14 2.15 2.16 2.17 2.18 2.19 2.20 2.21 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ 4.0 4.1 4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
  6. ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  7. ↑ Fernández De Henestrosa, AR et al. (2000) Identification of additional genes belonging to the LexA regulon in Escherichia coli. Mol. Microbiol. 35 1560-72 PubMed
  8. ↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

Categories

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