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W3110: 2822425..2821364<br /><gbrowseImage>
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{{GBrowseLink|W3110|AC_000091|2820364|2823425|GBrowse(W3110)}}  
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{{GBrowseFastaLink|W3110|AC_000091|2821364|2822425|FASTA Dump}}  
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{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="M46a9e41491853"  class=" tableEdit Gene_sequence_features_table"   
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{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="Z46a9e414d7fcb"  class=" tableEdit Gene_allele_table dataTable"   
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!|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments
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&Delta;recA (Keio:JW2669)
''recA(del)'' (Keio:JW2669)
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deletion
deletion
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PMID:16738554
PMID:16738554
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[http://www.shigen.nig.ac.jp/ecoli/strain/keioCollectionQueryAction.do Shigen]<br>[http://cgsc2.biology.yale.edu/Strain.php?ID=108386 CGSC10105]<ref name='LIB:cgsc'/>  
[http://www.shigen.nig.ac.jp/ecoli/strain/keioCollectionQueryAction.do Shigen]<br />[http://cgsc2.biology.yale.edu/Strain.php?ID=108386 CGSC10105]<ref name='LIB:cgsc'/>  
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&Delta;recA::kan
''recA(del)''::kan
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deletion
deletion-replacement
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&Delta;recA::kan
''recA(del)::kan''
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deletion
deletion
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&Delta;recA::kan
''recA(del)::kan''
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deletion
deletion
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''&#916;recA635::kan''
''recA635(del)::kan''
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''&#916;recA636''(del-ins)''::FRT''
''recA636''(del-ins)''::FRT''
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''&#916;recA774::kan''
''recA774(del)::kan''
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recA
''recA''
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deletion
deletion
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Sensitivity to
Sensitivity to
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increased sensitivity to [[bicyclomycin]]
increased sensitivity to [[Bicyclomycin]]
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PMID:21357484
PMID:21357484
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fig 2  
fig 2  
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''recA(del)''
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deletion
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Growth Phenotype
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When the ''recA'' gene was deleted there was only a marginal change in the growth rate compared to the wild type when exposed to the same concentration of levofloxacin.
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PMID:20660686
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See figure 1. 
|-
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''recA(del)''
|
The ''recA'' was deleted.
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Resistant to
|
After the ''recA'' gene was deleted there was only a marginal difference in the level of resistance compared to the wild type strain when exposed to the same concentration of levofloxacin.
|
PMID:20660686
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Figure 1B. 
|-
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''recA(del)''
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The ''recA'' gene was deleted.
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Resistant to
|
The number of ''recA(del)'' levofloxacin resistant mutants was fewer than the wildtype and did not appear as quickly.
|
PMID:20660686
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|
See Figure 4. 
|-
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''recA(del)''
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The ''recA'' gene was deactivated.
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Sensitivity to
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Mutant frequency decreased with the introduction of sublethal dosages of antibiotics.
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PMID:21212055
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|
See Figure 1
|-
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''recA(del)''
|
The recA gene was deactivated.
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Rate transcription
|
the rate of transcription of ''recA::GFP'' was increased with the additon antimicrobials
|
PMID:21212055
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See figure 2
|-
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''recA(del)''
|
The ''recA'' gene was deactivated.
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Growth Phenotype
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The addition of the antimicrobials ciprofloxacin, trimethoprim and sulfamethoxazole to the ''recA'' deficient mutant caused the formation of reduced filaments.
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PMID:21212055
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See figure 3(b)
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''nrdA101recA(del)''
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'recA'' is deactivated.
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rate of replication
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Replication is stalled when the replication forks are reversed due to the absence of ''recA''
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PMID:21441507
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|-
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''recA(del)''
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the ''recA'' gene was deactiaved.
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Sensitivity to
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By deactivating the ''recA'' gene there is an increase in sensitvity to UV radiation.
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PMID:2112525
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See Figure 2 A. 
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''recA(del)''
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the ''recA'' gene was deactiaved.
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Rate of Recombination
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By deactivating the ''recA'' gene there is a decrease in the rate of recombination frequency compared to the wildtype strain.
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PMID:2112525
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See Figure 2 B. 
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''recA(del)''
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the ''recA'' gene was deactiaved.
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rate of ''LexA'' cleavage
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By deactivating the ''recA'' gene there is a decrease ''LexA'' cleavage when compared to the wildtype.
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PMID:2112525
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See Figure 2 C. 
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''recA142''
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Sensitivity to
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mutants are more sensitive to DNA damaging agent Mitomycin C, fig 2.
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PMID:17040104
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''redA'' in strain F19
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Resistant to
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Resistant to Muteonidazole resistance
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PMID:1991710
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Strain: F19
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Figure 1


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!|Database!!Accession!!Notes
!|Database!!Accession!!Notes
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[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])
[[EcoCyc]]
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EcoCyc:EG10823
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[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=EG10823 ECOCYC:EG10823]
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]  
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Escherichia coli str. K-12 substr. MG1655  
[[EcoGene]]
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EcoGene:EG10823
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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[http://ecogene.org EcoGene] ([[EcoGene|EcoliWiki Page]])
[[RegulonDB]]
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[http://ecogene.org/geneinfo.php?eg_id=EG10823 EcoGene:EG10823]
RegulonDB:ECK120000814
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Escherichia coli str. K-12 substr. MG1655  
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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Escherichia coli str. K-12 substr. MG1655  
Escherichia coli str. K-12 substr. MG1655  
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[[EchoBASE]]
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EchoBASE:EB0816
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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[[ASAP]]
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ASAP:ABE-0008876
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]


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Latest revision as of 20:43, 19 September 2013

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

recA

Mnemonic

Recombination

Synonyms

ECK2694, b2699, JW2669, lexB, srf, umuB, zab, recH, rnmB, tif, umuR[1], umuR

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

60.8 minutes 

MG1655: 2821791..2820730
<gbrowseImage> name=NC_000913:2820730..2821791 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 2718628..2717567
<gbrowseImage> name=NC_012967:2717567..2718628 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 2706542..2707603
<gbrowseImage> name=NC_012759:2706542..2707603 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 2822425..2821364
<gbrowseImage> name=NC_007779:2821364..2822425 source=W3110 preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

2820733

Edman degradation

PMID:6244554[2]
PMID:6930655[3]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

Strain DH10B

G161D

Nonsynonomous mutation

PMID:18245285[4]

ASAP

This is a SNP in E. coli K-12 Strain DH10B

recA(del) (Keio:JW2669)

deletion

deletion

PMID:16738554[5]

Shigen
CGSC10105[6]

recA::Tn5KAN-I-SceI (FB20884)

Insertion at nt 407 in Plus orientation

PMID:15262929[7]

E. coli Genome Project:FB20884

does not contain pKD46

recA::Tn5KAN-I-SceI (FB20885)

Insertion at nt 407 in Plus orientation

PMID:15262929[7]

E. coli Genome Project:FB20885

contains pKD46

recA(del)::kan

deletion-replacement

Biolog:respiration

unable to respire a-Ketoglutarate

PMID:16095938[8]

recA(del)::kan

deletion

Biolog:respiration

unable to respire Succinate

PMID:16095938[8]

recA(del)::kan

deletion

Biolog:respiration

unable to respire L-Asparagine

PMID:16095938[8]

recA938::Tn9-200

CGSC:4556

recA1

PMID:6363880[9] PMID:350827[10]

CGSC:4691

recA56

PMID:2651400[11]

CGSC:4795

recA13

PMID:2651400[11]

CGSC:4897

recA44(ts)

temperature sensitive

PMID:2651400[11]

CGSC:5323

recA41

CGSC:5549

recA441(ts)

temperature sensitive

PMID:6363880[9] PMID:2406267[12] PMID:350827[10]

CGSC:6585

recA430

PMID:6363880[9] PMID:2651400[11] PMID:1400177[13]

CGSC:6772

recA3

CGSC:6961

recA11

PMID:7512687[14] PMID:5321955[15] PMID:350827[10]

CGSC:7623

recA200(ts)

temperature sensitive

CGSC:8413

recA14

CGSC:9230

recA12

PMID:7512687[14] PMID:350827[10]

CGSC:9257

recA431

CGSC:9898

recA34

CGSC:9900

recA99(Am)

PMID:350827[10] PMID:2651400[11]

CGSC:10336

amber (UAG) mutation

recA142

PMID:2651400[11]

CGSC:11217

recA111

CGSC:14572

recA629

PMID:6088537[16]

CGSC:19643

recA2111::RP4-2Tc::Mu

CGSC:60624

recA635(del)::kan

PMID:10829079[17]

CGSC:64884

recA636(del-ins)::FRT

PMID:10829079[17]

CGSC:65682

recA2

CGSC:72687

recA1907::cat-aadA

PMID:2155195[18]

CGSC:99541

recA774(del)::kan

PMID:16738554[5]

CGSC:102620

recA730

constitutively activated form of RecA so it cleaves the LexA repressor in the absence of SOS induction

PMID:2834329[19]

recA

deletion

Sensitivity to

increased sensitivity to Bicyclomycin

PMID:21357484[20]

fig 2

recA(del)

deletion

Growth Phenotype

When the recA gene was deleted there was only a marginal change in the growth rate compared to the wild type when exposed to the same concentration of levofloxacin.

PMID:20660686[21]

See figure 1.

recA(del)

The recA was deleted.

Resistant to

After the recA gene was deleted there was only a marginal difference in the level of resistance compared to the wild type strain when exposed to the same concentration of levofloxacin.

PMID:20660686[21]

Figure 1B.

recA(del)

The recA gene was deleted.

Resistant to

The number of recA(del) levofloxacin resistant mutants was fewer than the wildtype and did not appear as quickly.

PMID:20660686[21]

See Figure 4.

recA(del)

The recA gene was deactivated.

Sensitivity to

Mutant frequency decreased with the introduction of sublethal dosages of antibiotics.

PMID:21212055[22]

See Figure 1

recA(del)

The recA gene was deactivated.

Rate transcription

the rate of transcription of recA::GFP was increased with the additon antimicrobials

PMID:21212055[22]

See figure 2

recA(del)

The recA gene was deactivated.

Growth Phenotype

The addition of the antimicrobials ciprofloxacin, trimethoprim and sulfamethoxazole to the recA deficient mutant caused the formation of reduced filaments.

PMID:21212055[22]

See figure 3(b)

nrdA101recA(del)

'recA is deactivated.

rate of replication

Replication is stalled when the replication forks are reversed due to the absence of recA

PMID:21441507[23]

recA(del)

the recA gene was deactiaved.

Sensitivity to

By deactivating the recA gene there is an increase in sensitvity to UV radiation.

PMID:2112525[24]

See Figure 2 A.

recA(del)

the recA gene was deactiaved.

Rate of Recombination

By deactivating the recA gene there is a decrease in the rate of recombination frequency compared to the wildtype strain.

PMID:2112525[24]

See Figure 2 B.

recA(del)

the recA gene was deactiaved.

rate of LexA cleavage

By deactivating the recA gene there is a decrease LexA cleavage when compared to the wildtype.

PMID:2112525[24]

See Figure 2 C.

recA142

Sensitivity to

mutants are more sensitive to DNA damaging agent Mitomycin C, fig 2.

PMID:17040104[25]

redA in strain F19

Resistant to

Resistant to Muteonidazole resistance

PMID:1991710[26]

Strain: F19

Figure 1

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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW2669

Plasmid clone

Shigen

PMID:16769691[27]

Status:Clone OK

Primer 1:GCCGCTATCGACGAAAACAAACA

Primer 2:CCAAAATCTTCGTTAGTTTCTGC

23B7

Kohara Phage

Genobase

PMID:3038334[28]

pheA18::Tn10

Linked marker

CAG12158 = CGSC7421[6]

est. P1 cotransduction: % [29]

srlD3131::Tn10

Linked marker

CAG18642 = CGSC7423[6]

est. P1 cotransduction: 85% [29]
Synonyms:zfh-3131::Tn10, zfi-3131::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10823

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10823

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000814

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:947170

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0816

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0008876

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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<protect>

References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ Horii, T et al. (1980) Organization of the recA gene of Escherichia coli. Proc. Natl. Acad. Sci. U.S.A. 77 313-7 PubMed
  3. ↑ Sancar, A et al. (1980) Sequences of the recA gene and protein. Proc. Natl. Acad. Sci. U.S.A. 77 2611-5 PubMed
  4. ↑ Durfee, T et al. (2008) The complete genome sequence of Escherichia coli DH10B: insights into the biology of a laboratory workhorse. J. Bacteriol. 190 2597-606 PubMed
  5. ↑ 5.0 5.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  6. ↑ 6.0 6.1 6.2 CGSC: The Coli Genetics Stock Center
  7. ↑ 7.0 7.1 Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
  8. ↑ 8.0 8.1 8.2 Ito, M et al. (2005) Functional analysis of 1440 Escherichia coli genes using the combination of knock-out library and phenotype microarrays. Metab. Eng. 7 318-27 PubMed
  9. ↑ 9.0 9.1 9.2 Kawashima, H et al. (1984) Functional domains of Escherichia coli recA protein deduced from the mutational sites in the gene. Mol. Gen. Genet. 193 288-92 PubMed
  10. ↑ 10.0 10.1 10.2 10.3 10.4 Templin, A et al. (1978) Suppressibility of recA, recB, and recC mutations by nonsense suppressors. J. Bacteriol. 134 590-6 PubMed
  11. ↑ 11.0 11.1 11.2 11.3 11.4 11.5 Dutreix, M et al. (1989) New recA mutations that dissociate the various RecA protein activities in Escherichia coli provide evidence for an additional role for RecA protein in UV mutagenesis. J. Bacteriol. 171 2415-23 PubMed
  12. ↑ Lavery, PE & Kowalczykowski, SC (1990) Properties of recA441 protein-catalyzed DNA strand exchange can be attributed to an enhanced ability to compete with SSB protein. J. Biol. Chem. 265 4004-10 PubMed
  13. ↑ Larminat, F et al. (1992) New mutations in and around the L2 disordered loop of the RecA protein modulate recombination and/or coprotease activity. J. Bacteriol. 174 6264-9 PubMed
  14. ↑ 14.0 14.1 Zaitsev, E et al. (1994) Nucleotide sequence between recA and alaSp in E. coli K12 and the sequence change in four recA mutations. Mutat. Res. 323 173-7 PubMed
  15. ↑ Fuerst, CR & Siminovitch, L (1965) Characterization of an unusual defective lysogenic strain of Escherichia coli K-12(lambda). Virology 27 449-51 PubMed
  16. ↑ Knight, KL et al. (1984) Identification of the amino acid substitutions in two mutant forms of the recA protein from Escherichia coli: recA441 and recA629. J. Biol. Chem. 259 11279-83 PubMed
  17. ↑ 17.0 17.1 Datsenko, KA & Wanner, BL (2000) One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc. Natl. Acad. Sci. U.S.A. 97 6640-5 PubMed
  18. ↑ Wanner, BL & Boline, JA (1990) Mapping and molecular cloning of the phn (psiD) locus for phosphonate utilization in Escherichia coli. J. Bacteriol. 172 1186-96 PubMed
  19. ↑ Lin, LL & Little, JW (1988) Isolation and characterization of noncleavable (Ind-) mutants of the LexA repressor of Escherichia coli K-12. J. Bacteriol. 170 2163-73 PubMed
  20. ↑ Tran, L et al. (2011) Single-gene deletion mutants of Escherichia coli with altered sensitivity to bicyclomycin, an inhibitor of transcription termination factor Rho. J. Bacteriol. 193 2229-35 PubMed
  21. ↑ 21.0 21.1 21.2 Singh, R et al. (2010) Impact of recA on levofloxacin exposure-related resistance development. Antimicrob. Agents Chemother. 54 4262-8 PubMed
  22. ↑ 22.0 22.1 22.2 Thi, TD et al. (2011) Effect of recA inactivation on mutagenesis of Escherichia coli exposed to sublethal concentrations of antimicrobials. J. Antimicrob. Chemother. 66 531-8 PubMed
  23. ↑ Salguero, I et al. (2011) RecA-dependent replication in the nrdA101(Ts) mutant of Escherichia coli under restrictive conditions. J. Bacteriol. 193 2851-60 PubMed
  24. ↑ 24.0 24.1 24.2 Harada, F et al. (1990) A simple, general method for detecting retroviral RNAs expressed in cells. Jpn. J. Cancer Res. 81 232-7 PubMed
  25. ↑ Vidal, LS et al. (2006) Enhanced sensitivity of Escherichia coli uvrB mutants to mitomycin C points to a UV-C distinct repair for DNA adducts. Chem. Res. Toxicol. 19 1351-6 PubMed
  26. ↑ Santangelo, JD et al. (1991) Metronidazole activation and isolation of Clostridium acetobutylicum electron transport genes. J. Bacteriol. 173 1088-95 PubMed
  27. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  28. ↑ Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  29. ↑ 29.0 29.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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