rep:Gene: Difference between revisions

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|{{Sub}}[[#Nomenclature |Nomenclature]]
|{{Sub}}[[#Nomenclature |Nomenclature]]
|{{Sub}}[[#Location(s) and DNA Sequence|Location(s) and DNA Sequence]]
|{{Sub}}[[#Location(s) and DNA Sequence|Location(s) and DNA Sequence]]
|{{Sub}}[[#Sequence Features|Sequence Features]]
|{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]]
|{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]]
|{{Sub}}[[#Genetic Interactions | Genetic Interactions]]
|{{Sub}}[[#Genetic Resources  |Genetic Resources]]
|{{Sub}}[[#Genetic Resources  |Genetic Resources]]
|{{Sub}}[[#Accessions in Other Databases |Accessions]]
|{{Sub}}[[#Accessions in Other Databases |Accessions]]
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****************************************************************************************** -->{{Gene_nomenclature_help}}
{|{{Prettytable}}
{|   id="X46a9feac3337d"  class=" tableEdit Gene_nomenclature_table" 
 
|-
!align=left {{table_heading_style}} |Standard name
||
''rep''
|-
|-
!{{table_heading_style}} |Standard name
!align=left {{table_heading_style}} |Mnemonic
|
||
''rep''
Replicase
|-
|-
!{{table_heading_style}} |Mnemonic
!align=left {{table_heading_style}} |Synonyms
|
||
''ECK3770, b3778, JW5604, dasC, mbrA, mmrA, dasC?''<ref name='LIB:Riley_2006'/><ref name='LIB:EcoCyc10.6'/>, mmrA?''


|-
|- class="tableEdit_footer"
!{{table_heading_style}} |Synonyms
|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.18115.X46a9feac3337d&page=18115&pagename={{FULLPAGENAMEE}}&type=1&template=Gene_nomenclature_table edit table]</span> ||
|
''ECK3770, b3778, JW5604, dasC, mbrA, mmrA, dasC?''<ref name='LIB:Riley_2006'/><ref name='LIB:EcoCyc10.6'/>
|-
|colspan='2'|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.18115.X46a9feac3337d&page=18115&pagename={{FULLPAGENAMEE}} edit table]
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<!--Nomenclature_notes--></protect>
<!--Nomenclature_notes--></protect>
===Notes===
===Notes===
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{|class = 'sortable' {{Prettytable}} id='147549'
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="N46a9feacb6204"  class=" tableEdit Gene_location_table" 
|- {{table_heading_style}}
|- {{table_heading_style}}
!Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links
!|Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links
|-
|-  
|
|
MG1655
MG1655
Line 72: Line 76:
85.32 minutes&nbsp;
85.32 minutes&nbsp;
|
|
MG1655: 3958700..3960721<br />{{GBrowseFigureByCoord|3957700|3961721|ecoli|MG1655}}
MG1655: 3958700..3960721<br /><gbrowseImage>
name=NC_000913:3958700..3960721
source=MG1655
preset=GeneLocation
</gbrowseImage>
 
|
|
*[http://biocyc.org/ECOLI/NEW-IMAGE?type=LOCUS-POSITION&chromosome=COLI-K12&object=EG10837 EcoCyc]
*[http://biocyc.org/ECOLI/NEW-IMAGE?type=LOCUS-POSITION&chromosome=COLI-K12&object=EG10837 EcoCyc]
{{GBrowseLink|ecoli|NC_000913|3957700|3961721|GBrowse(MG1655)}}
{{GBrowseLink|MG1655|NC_000913|3957700|3961721|GBrowse(MG1655)}}
   
   
|
|
*[http://dimer.tamu.edu/cgi-bin/gb/gbrowse/ecoli/?plugin=FastaDumper&name=MG1655:3958700..3960721&plugin_action=Go FASTA Dump]
{{GBrowseFastaLink|MG1655|NC_000913|3958700|3960721|FASTA Dump}}
*[http://biocyc.org/ECOLI/sequence?object=EG10837 EcoCyc Sequence Dump]  
*[http://biocyc.org/ECOLI/sequence?object=EG10837 EcoCyc Sequence Dump]  
|-
|-
|
[[REL606]]
|
 
|
NC_012967: 3922479..3924500<br /><gbrowseImage>
name=NC_012967:3922479..3924500
source=REL606
preset=GeneLocation
</gbrowseImage>
 
|
{{GBrowseLink|REL606|NC_012967|3922479|3924500|GBrowse (REL606)}}
|
{{GBrowseFastaLink|REL606|NC_012967|3922479|3924500|FASTA Dump}}
|-
|
[[BW2952]]
|
 
|
NC_012759: 3848369..3850390<br /><gbrowseImage>
name=NC_012759:3848369..3850390
source=BW2952
preset=GeneLocation
</gbrowseImage>
 
|
{{GBrowseLink|BW2952|NC_012759|3848369|3850390|GBrowse (BW2952)}}
|
{{GBrowseFastaLink|BW2952|NC_012759|3848369|3850390|FASTA Dump}}
|-  
|
|
W3110
W3110
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&nbsp;
&nbsp;
|
|
W3110: 3676004..3673983<br />{{GBrowseFigureByCoord|3672983|3677004|ecoli_W3110|W3110}}
W3110: 3676004..3673983<br /><gbrowseImage>
name=NC_007779:3673983..3676004
source=W3110
preset=GeneLocation
</gbrowseImage>
 
|
|
*[http://dimer.tamu.edu/cgi-bin/gb/gbrowse/ecoli_W3110/?q=W3110:3672983..3677004 GBrowse(W3110)]
{{GBrowseLink|W3110|NC_007779|3672983|3677004|GBrowse(W3110)}}
|
|
*[http://dimer.tamu.edu/cgi-bin/gb/gbrowse/ecoli_W3110/?plugin=FastaDumper&name=W3110:3673983..3676004&plugin_action=Go FASTA Dump]
{{GBrowseFastaLink|W3110|NC_007779|3673983|3676004|FASTA Dump}}
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[[DH10B]]
|
 
|
DH10B: 4057620..4059641<br /><gbrowseImage>
name=NC_010473:4057620..4059641
source=DH10B
preset=GeneLocation
</gbrowseImage>
 
|
{{GBrowseLink|DH10B|NC_010473|4053578|4063683|GBrowse(DH10B)}}
|
{{GBrowseFastaLink|DH10B|NC_010473|4053578|4063683|FASTA Dump}}
 
|- class="tableEdit_footer"
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===Notes===
===Notes===
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{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="I46a9fead7adba"  class=" tableEdit Gene_sequence_features_table" 
|- {{table_heading_style}}
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!Feature type!!Location!!Description
!|Feature Type!!Strain!!Genomic Location!!Evidence!!Reference!!Notes
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Coding Start (SO:0000323)
|
[[MG1655]]
|
3958700
|
Edman degradation
|
PMID:2999067<br />
|
 
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<protect><!--Sequence_notes--></protect>
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====Notes====
====Notes====
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{|class = 'sortable' border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" {{Prettytable}}
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="Y46a9feadc506b"  class=" tableEdit Gene_allele_table dataTable" 
|- {{table_heading_style}}
|- {{table_heading_style}}
!|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments
!|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments
|-  
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|
|
&Delta;rep (Keio:JW5604)
''rep(del)'' (Keio:JW5604)
|
|
deletion
deletion
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|
|
[[PMID:16738554]]<ref name=PMID:16738554/>
PMID:16738554
|
|
[http://www.shigen.nig.ac.jp/ecoli/strain/keioCollectionQueryAction.do Shigen]
[http://www.shigen.nig.ac.jp/ecoli/strain/keioCollectionQueryAction.do Shigen]
|
|-
|
[http://asap.ahabs.wisc.edu/asap/feature_info.php?FeatureID=ABE-0012344 rep::Tn5KAN-I-SceI] (FB21451)
|
Insertion at nt 508 in Minus orientation
|
|
|
|
PMID:15262929
|
[http://www.genome.wisc.edu/resources/forms/strainrequest.htm E. coli Genome Project:FB21451]
|
contains [[pKD46]]
|-
|
&Delta;rep::kan
|
deletion
|
|
[[Biolog]]:respiration
|
unable to respire [[Carbon sources|Acetate]]
|
PMID:16095938
|
|
|-
|
&Delta;rep::kan
|
deletion
|
|
[[Biolog]]:respiration
|
unable to respire [[Carbon sources|a-Hydroxybutyrate]]
|
PMID:16095938
|
|
|-
|
&Delta;rep::kan
|
deletion
|
|
[[Biolog]]:respiration
|
unable to respire [[Carbon sources|a-Ketobutyrate]]
|
PMID:16095938
|
|
|-
|
&Delta;rep::kan
|
deletion
|
|
[[Biolog]]:respiration
|
unable to respire [[Carbon sources|a-Ketoglutarate]]
|
PMID:16095938
|
|
|-
|
&Delta;rep::kan
|
deletion
|
|
[[Biolog]]:respiration
|
unable to respire [[Carbon sources|Succinate]]
|
PMID:16095938
|
|
|-
|
&Delta;rep::kan
|
deletion
|
|
[[Biolog]]:respiration
|
unable to respire [[Carbon sources|Bromosuccinate]]
|
PMID:16095938
|
|
|-
|
&Delta;rep::kan
|
deletion
|
|
[[Biolog]]:respiration
|
unable to respire [[Carbon sources|L-Alanine]]
|
PMID:16095938
|
|
|-
|
&Delta;rep::kan
|
deletion
|
|
[[Biolog]]:respiration
|
unable to respire [[Carbon sources|L-Asparagine]]
|
PMID:16095938
|
|
|-
|
&Delta;rep::kan
|
deletion
|
|
[[Biolog]]:respiration
|
unable to respire [[Carbon sources|L-Aspartate]]
|
PMID:16095938
|
|
|-
|
&Delta;rep::kan
|
deletion
|
|
[[Biolog]]:respiration
|
unable to respire [[Carbon sources|L-Serine]]
|
PMID:16095938
|
|
|
   
   
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|
|
[[PMID:383240]]<ref name=PMID:383240/>
PMID:383240
|
|
[[cgscmutation:9794|CGSC:9794]]
[[cgscmutation:9794|CGSC:9794]]
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|
|
[[PMID:6286604]]<ref name=PMID:6286604/>
PMID:6286604
|
|
[[cgscmutation:90823|CGSC:90823]]
[[cgscmutation:90823|CGSC:90823]]
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|-  
|-  
|
|
''&#916;rep-729::kan''
''rep-729(del)::kan''
|
|


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|
|
[[PMID:16738554]]<ref name=PMID:16738554/>
PMID:16738554
|
|
[[cgscmutation:113537|CGSC:113537]]
[[cgscmutation:113537|CGSC:113537]]
|
|
   
   
|-
|
rep in [[MDS42]]
|
deletion
|


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Sensitivity to
|
increased sensitivity to [[Bicyclomycin]]
|
PMID:21183718
|
 
|
Fig. 2
|-
|
mbrA4
|
 
|
 
|
Growth Phenotype
|
no growth in the first few hours at non-permissive conditions but eventually resumed growth, after exposed to LB cells began to elongate
LB-Mac-
|
PMID:2176633
|
 
|
|-
|
mbrA15
|
 
|
 
|
Growth Phenotype
|
temperature sensitive, forms two distinct populations, one more dense and one less dense than wild type
|
PMID:2176633
|
 
|
|-
|
''mbrA4''
|
 
|
 
|
Resistant to
|
camphor resistance
|
PMID:2176633
 
PMID:1925019
|
 
|
Table 2 and table 1
|-
|
''mrbA15''
|
 
|
 
|
Resistant to
|
Resistant to camphor
|
PMID:2176633
 
PMID:1925019
|
 
|
table 2 and table 1
 
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{{Gene_alleles_help}}
 
<protect><!--Alleles_notes--></protect>
<protect><!--Alleles_notes--></protect>
===Notes===
===Notes===


==Genetic Interactions==
{{BacktoTop}}
<protect><!--box uid=bc145ea147f7e36caf09c1bfeed4ee9d.18115.G4e04c23ae6338-->
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{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="G4e04c23ae6338"  class=" tableEdit Genetic_interaction_table dataTable" 
|- {{table_heading_style}}
!|Interactor!!Interaction!!Allele!!Score(s)!!Reference(s)!!Accessions!!Notes
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===Notes===


==Genetic Resources==
==Genetic Resources==
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JW5604
JW5604
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[http://www.shigen.nig.ac.jp/ecoli/strain/askaCloneQueryAction.do?strainTypeId=9 Shigen]
[http://www.shigen.nig.ac.jp/ecoli/strain/askaCloneQueryAction.do?strainTypeId=9 Shigen]
|
|
[[PMID:16769691]]<ref name=PMID:16769691/>
PMID:16769691


Status:cloned as old JW
Status:cloned as old JW
Line 272: Line 648:


Primer 2:CCTTTCCCTCGTTTTGCCGCCAT  
Primer 2:CCTTTCCCTCGTTTTGCCGCCAT  
|-
|-
|
[[:Category:Kohara_Phage:2A8|2A8]]
|
Kohara Phage
|
[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=556 Genobase]
|
PMID:3038334
|-
|
[[:Category:Kohara_Phage:2E6|2E6]]
|
Kohara Phage
|
[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=557 Genobase]
|
PMID:3038334
|-  
|
|
[[ilvD:Gene|ilvD500::Tn10]]
[[ilvD:Gene|ilvD500::Tn10]]
Line 280: Line 674:
CAG18431 = [http://cgsc2.biology.yale.edu/Strain.php?ID=40338 CGSC7462]<ref name='LIB:cgsc'/>
CAG18431 = [http://cgsc2.biology.yale.edu/Strain.php?ID=40338 CGSC7462]<ref name='LIB:cgsc'/>
|
|
est. P1 cotransduction: 83% <ref name='LIB:Nichols_linked_Tn'/>
est. P1 cotransduction: 83% <ref name='LIB:Nichols_linked_Tn'/> <br />
 
Synonyms:ilv500::Tn10  
Synonyms:ilv500::Tn10  
|-
|-  
|
|
metEo-3079::Tn10
metEo-3079::Tn10
Line 291: Line 684:
CAG18491 = [http://cgsc2.biology.yale.edu/Strain.php?ID=40350 CGSC7464]<ref name='LIB:cgsc'/>
CAG18491 = [http://cgsc2.biology.yale.edu/Strain.php?ID=40350 CGSC7464]<ref name='LIB:cgsc'/>
|
|
est. P1 cotransduction: 10% <ref name='LIB:Nichols_linked_Tn'/>
est. P1 cotransduction: 10% <ref name='LIB:Nichols_linked_Tn'/> <br />
 
Synonyms:metE3079::Tn10 nnnThe Tn10 in CAG13491 is inserted in the metE regulatory region, but causes a Met- phenotype.
Synonyms:metE3079::Tn10  
 


The Tn10 in CAG13491 is inserted in the metE regulatory region, but causes a Met- phenotype.
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===Notes===
===Notes===
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{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="J46a9feaf34a80"  class=" tableEdit Gene_accessions_table"   
|- {{table_heading_style}}
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!|Database!!Accession!!Notes
!|Database!!Accession!!Notes
|-  
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|
[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])
[[EcoCyc]]
|
|
[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=EG10837 EcoCyc:EG10837]
EcoCyc:EG10837
|
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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[http://www.ecoli-york.org EchoBASE] ([[EchoBASE|EcoliWiki Page]])
[[EcoGene]]
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[http://www.york.ac.uk/res/thomas/Gene.cfm?recordID=EB0830 EchoBASE:EB0830]
EcoGene:EG10837
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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[http://chase.ou.edu/oubcf/index.php EcoliGenExpDB] ([[EcoliGenExpDB|EcoliWiki Page]])
[[RegulonDB]]
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[http://chase.ou.edu/macro/searchlink.php?bnum=b3778 EcoliGenExpDB:b3778]
RegulonDB:ECK120000828
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]  
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[http://BioCyc.org/ECOL199310/ ECOL199310CYC] ([[ECOL199310CYC|EcoliWiki Page]])
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[http://BioCyc.org/ECOL199310/new-image?object=C4698 ECOL199310CYC:C4698]
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[http://ecogene.org EcoGene] ([[EcoGene|EcoliWiki Page]])
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[http://ecogene.org/geneinfo.php?eg_id=EG10837 EcoGene:EG10837]
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[http://BioCyc.org/ECOO157/ ECOO157CYC] ([[ECOO157CYC|EcoliWiki Page]])  
[http://www.ncbi.nlm.nih.gov/gene NCBI] ([[NCBI|EcoliWiki Page]])
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[http://BioCyc.org/ECOO157/new-image?object=REP ECOO157CYC:REP]
[http://www.ncbi.nlm.nih.gov/gene?term=948292 GeneID:948292]
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Escherichia coli str. K-12 substr. MG1655
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[http://cgsc.biology.yale.edu CGSC] ([[CGSC|EcoliWiki Page]])
[[EchoBASE]]
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[http://cgsc.biology.yale.edu/cgi-bin/sybgw/cgsc/Site/303 CGSC:303]
EchoBASE:EB0830
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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[https://asap.ahabs.wisc.edu/asap/logon.php ASAP] ([[ASAP|EcoliWikiPage]])
[[ASAP]]
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[http://asap.ahabs.wisc.edu/asap/feature_info.php?LocationID=WIS&FeatureID=0012344&formSubmitReturn=1 ASAP:ABE-0012344] 
ASAP:ABE-0012344
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]


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===Categories===
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[[Category:Gene List:MG1655]]
[[Category:Gene List:MG1655]]
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[[Category:Genes in the Keio knockout collection]]
[[Category:Genes in the Keio knockout collection]]
[[Category:Genes in the ASKA clone collection]]
[[Category:Genes in the ASKA clone collection]]
[[Category:rep genes]]
[[Category:rep genes]][[Category:Gene_List:DH10B]][[Category:Gene_List:REL606]]
[[Category:Gene_List:BW2952]]

Latest revision as of 20:49, 19 September 2013

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

rep

Mnemonic

Replicase

Synonyms

ECK3770, b3778, JW5604, dasC, mbrA, mmrA, dasC?[1][2], mmrA?

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

85.32 minutes 

MG1655: 3958700..3960721
<gbrowseImage> name=NC_000913:3958700..3960721 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 3922479..3924500
<gbrowseImage> name=NC_012967:3922479..3924500 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 3848369..3850390
<gbrowseImage> name=NC_012759:3848369..3850390 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 3676004..3673983
<gbrowseImage> name=NC_007779:3673983..3676004 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 4057620..4059641
<gbrowseImage> name=NC_010473:4057620..4059641 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

3958700

Edman degradation

PMID:2999067[3]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

rep(del) (Keio:JW5604)

deletion

deletion

PMID:16738554[4]

Shigen

rep::Tn5KAN-I-SceI (FB21451)

Insertion at nt 508 in Minus orientation

PMID:15262929[5]

E. coli Genome Project:FB21451

contains pKD46

Δrep::kan

deletion

Biolog:respiration

unable to respire Acetate

PMID:16095938[6]

Δrep::kan

deletion

Biolog:respiration

unable to respire a-Hydroxybutyrate

PMID:16095938[6]

Δrep::kan

deletion

Biolog:respiration

unable to respire a-Ketobutyrate

PMID:16095938[6]

Δrep::kan

deletion

Biolog:respiration

unable to respire a-Ketoglutarate

PMID:16095938[6]

Δrep::kan

deletion

Biolog:respiration

unable to respire Succinate

PMID:16095938[6]

Δrep::kan

deletion

Biolog:respiration

unable to respire Bromosuccinate

PMID:16095938[6]

Δrep::kan

deletion

Biolog:respiration

unable to respire L-Alanine

PMID:16095938[6]

Δrep::kan

deletion

Biolog:respiration

unable to respire L-Asparagine

PMID:16095938[6]

Δrep::kan

deletion

Biolog:respiration

unable to respire L-Aspartate

PMID:16095938[6]

Δrep::kan

deletion

Biolog:respiration

unable to respire L-Serine

PMID:16095938[6]

rep-5

CGSC:7013

rep-3

PMID:383240[7]

CGSC:9794

rep-142(ts)

temperature sensitive

CGSC:90737

[rep-71]

PMID:6286604[8]

CGSC:90823

null allele

rep-729(del)::kan

PMID:16738554[4]

CGSC:113537

rep in MDS42

deletion

Sensitivity to

increased sensitivity to Bicyclomycin

PMID:21183718[9]

Fig. 2

mbrA4

Growth Phenotype

no growth in the first few hours at non-permissive conditions but eventually resumed growth, after exposed to LB cells began to elongate LB-Mac-

PMID:2176633[10]

mbrA15

Growth Phenotype

temperature sensitive, forms two distinct populations, one more dense and one less dense than wild type

PMID:2176633[10]

mbrA4

Resistant to

camphor resistance

PMID:2176633[10]

PMID:1925019[11]

Table 2 and table 1

mrbA15

Resistant to

Resistant to camphor

PMID:2176633[10]

PMID:1925019[11]

table 2 and table 1

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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW5604

Plasmid clone

Shigen

PMID:16769691[12]

Status:cloned as old JW

Primer 1:GCCCGTCTAAACCCCGGCCAACA

Primer 2:CCTTTCCCTCGTTTTGCCGCCAT

2A8

Kohara Phage

Genobase

PMID:3038334[13]

2E6

Kohara Phage

Genobase

PMID:3038334[13]

ilvD500::Tn10

Linked marker

CAG18431 = CGSC7462[14]

est. P1 cotransduction: 83% [15]
Synonyms:ilv500::Tn10

metEo-3079::Tn10

Linked marker

CAG18491 = CGSC7464[14]

est. P1 cotransduction: 10% [15]
Synonyms:metE3079::Tn10 nnnThe Tn10 in CAG13491 is inserted in the metE regulatory region, but causes a Met- phenotype.

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10837

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10837

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000828

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:948292

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0830

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0012344

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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<protect>

References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ Bialkowska-Hobrzanska, H et al. (1985) Escherichia coli rep gene: identification of the promoter and N terminus of the rep protein. J. Bacteriol. 164 1004-10 PubMed
  4. ↑ 4.0 4.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  5. ↑ Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
  6. ↑ 6.00 6.01 6.02 6.03 6.04 6.05 6.06 6.07 6.08 6.09 Ito, M et al. (2005) Functional analysis of 1440 Escherichia coli genes using the combination of knock-out library and phenotype microarrays. Metab. Eng. 7 318-27 PubMed
  7. ↑ Takahashi, S et al. (1979) The rep mutation. VI. Purification and properties of the Escherichia coli rep protein, DNA helicase III. Can. J. Biochem. 57 855-66 PubMed
  8. ↑ Tessman, I et al. (1982) Relative map location of the rep and rho genes of Escherichia coli. J. Bacteriol. 151 1637-40 PubMed
  9. ↑ Washburn, RS & Gottesman, ME (2011) Transcription termination maintains chromosome integrity. Proc. Natl. Acad. Sci. U.S.A. 108 792-7 PubMed
  10. ↑ 10.0 10.1 10.2 10.3 Trun, NJ & Gottesman, S (1990) On the bacterial cell cycle: Escherichia coli mutants with altered ploidy. Genes Dev. 4 2036-47 PubMed
  11. ↑ 11.0 11.1 Trun, NJ & Gottesman, S () Characterization of Escherichia coli mutants with altered ploidy. Res. Microbiol. 142 195-200 PubMed
  12. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  13. ↑ 13.0 13.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  14. ↑ 14.0 14.1 CGSC: The Coli Genetics Stock Center
  15. ↑ 15.0 15.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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