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On One Page

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Gene |

Product(s) |

Expression|

Evolution |

References |

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Standard Name	tolC
Gene Synonym(s)	ECK3026, b3035, JW5503, colE1-i, mtcB, refI, weeA, mukA, toc^[1], toc
Product Desc.	TolC outer membrane channel^[2]^[3] Outer membrane factor (OMF) of tripartite efflux pumps; channel-tunnel spanning the outer membrane and periplasm; homotrimeric; ColE1 tolerance^[4]
Product Synonyms(s)	transport channel^[1], B3035^[2]^[1], WeeA^[2]^[1], Toc^[2]^[1], RefI^[2]^[1], MukA^[2]^[1], MtcB^[2]^[1], TolC^[2]^[1] , colE1-i, ECK3026, JW5503, mtcB, mukA, refI, toc, weeA, b3035
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): tolC^[2]
Regulation/Activity
Quick Links
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Notes

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TolC, MdtQ, MdtP and CusC are paralogs.^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	tolC
Mnemonic	Tolerance
Synonyms	ECK3026, b3035, JW5503, colE1-i, mtcB, refI, weeA, mukA, toc^[1], toc
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	68.46 minutes	MG1655: 3176137..3177618 <gbrowseImage> name=NC_000913:3176137..3177618 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 3112287..3113768 <gbrowseImage> name=NC_012967:3112287..3113768 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 3063285..3064766 <gbrowseImage> name=NC_012759:3063285..3064766 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 3176771..3178252 <gbrowseImage> name=NC_007779:3176771..3178252 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 3273882..3275363 <gbrowseImage> name=NC_010473:3273882..3275363 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
Coding Start (SO:0000323)	MG1655	3176203	Edman degradation	PMID:9298646 PMID:9629924
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Type	Phenotype: Description	Reference	Availability	Comments
ΔtolC (Keio:JW5503)	deletion	deletion			PMID:16738554	Shigen CGSC11430^[5]
ΔtolC::kan	deletion		Biolog:respiration	unable to respire Glycerol	PMID:16095938
ΔtolC::kan	deletion		Biolog:respiration	unable to respire DL-a-Glycerol	PMID:16095938
ΔtolC::kan	deletion		Biolog:respiration	unable to respire D-Galactoniate	PMID:16095938
ΔtolC::kan	deletion		Biolog:respiration	unable to respire D-Galacturonate	PMID:16095938
ΔtolC::kan	deletion		Biolog:respiration	unable to respire D-Glucuronate	PMID:16095938
ΔtolC::kan	deletion		Biolog:respiration	unable to respire Acetate	PMID:16095938
ΔtolC::kan	deletion		Biolog:respiration	unable to respire a-Ketoglutarate	PMID:16095938
ΔtolC::kan	deletion		Biolog:respiration	unable to respire Succinate	PMID:16095938
ΔtolC::kan	deletion		Biolog:respiration	unable to respire Bromosuccinate	PMID:16095938
ΔtolC::kan	deletion		Biolog:respiration	unable to respire L-Alanine	PMID:16095938
ΔtolC::kan	deletion		Biolog:respiration	unable to respire L-Alanyl-glycine	PMID:16095938
ΔtolC::kan	deletion		Biolog:respiration	unable to respire L-Asparagine	PMID:16095938
ΔtolC::kan	deletion		Biolog:respiration	unable to respire L-Aspartate	PMID:16095938
ΔtolC::kan	deletion		Biolog:respiration	unable to respire Glycyl-L-aspartate	PMID:16095938
ΔtolC::kan	deletion		Biolog:respiration	unable to respire D-Serine	PMID:16095938
ΔtolC::kan	deletion		Biolog:respiration	unable to respire L-Serine	PMID:16095938
ΔtolC::kan	deletion		Biolog:respiration	unable to respire Inosine	PMID:16095938
ΔtolC::kan	deletion		Biolog:respiration	unable to respire Uridine	PMID:16095938
tolC210::Tn10	Insertion at 3,176,395 bp in MG1655 (NC_000913)				adapted from Nichols et al.^[6]	CAG12184 = CGSC7437^[5]	Synonyms:
tolC3						CGSC:7871
ΔtolC5					PMID:4933702	CGSC:10139
tolC6::mini-Tn10						CGSC:10799
tolC210::Tn10					PMID:2540407	CGSC:38756
ΔtolC732::kan					PMID:16738554	CGSC:103166
mukA1			Growth Phenotype	anucleate cells, slower growth, hypersensitivity to sodium dodecyl sulfate, tolerance to colicin E1 protein defective in locating replicated and decanted chromosomes	PMID:2646284
tolC732::Kan			Auxotrophies	TolC was required for EXTREME-acid survival (pH 2) of strain W3110 grown aerobically to stationary phase. A tolC deletion decreased extreme-acid survival (acid resistance) of aerated pH 7.0-grown cells by 105-fold and of pH 5.5-grown cells by 10-fold. TolC was required for expression of glutamate decarboxylase (GadA, GadB), a key component of glutamate-dependent acid resistance (Gad). TolC was also required for maximal exponential growth of E. coli K-12 W3110, in LBK medium buffered at pH 4.5-6.0, but not at pH 6.5-8.5. The TolC growth requirement in moderate acid was independent of Gad.	PMID:21541325	Parent:W3110 expiramental: JLS1-15
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW5503	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCAAGAAATTGCTCCCCATTCT Primer 2:CCGTTACGGAAAGGGTTATGACC
17B2	Kohara Phage	Genobase	PMID:3038334
6B12	Kohara Phage	Genobase	PMID:3038334
metC162::Tn10	Linked marker	CAG18475 = CGSC7435^[5]	est. P1 cotransduction: 38% ^[6]
aer-3075::Tn10	Linked marker	CAG12152 = CGSC7438^[5]	est. P1 cotransduction: 19% ^[6] Synonyms:zgh-3075::Tn10, zgj-3075::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11009	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11009	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000998	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:947521	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1002	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0009965	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	TolC
Synonyms	transport channel^[1], B3035^[2]^[1], WeeA^[2]^[1], Toc^[2]^[1], RefI^[2]^[1], MukA^[2]^[1], MtcB^[2]^[1], TolC^[2]^[1] , colE1-i, ECK3026, JW5503, mtcB, mukA, refI, toc, weeA, b3035
Product description	TolC outer membrane channel^[2]^[3] Outer membrane factor (OMF) of tripartite efflux pumps; channel-tunnel spanning the outer membrane and periplasm; homotrimeric; ColE1 tolerance^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0042930	enterobactin transport	PMID:16166532	IGI: Inferred from Genetic Interaction	EcoliWiki:fur	P		complete
	GO:0014070	response to organic cyclic substance	PMID:9473050	IMP: Inferred from Mutant Phenotype		P		complete
	GO:0005215	transporter activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR003423	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005886	plasma membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-1003	C	Seeded from EcoCyc (v14.0)	complete
	GO:0009279	cell outer membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0998	C	Seeded from EcoCyc (v14.0)	complete
	GO:0015031	protein transport	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR010130	P	Seeded from EcoCyc (v14.0)	complete
	GO:0016021	integral to membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0812	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016021	integral to membrane	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-9909	C	Seeded from EcoCyc (v14.0)	complete
	GO:0009279	cell outer membrane	PMID:6337123	IDA: Inferred from Direct Assay		C		complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Evidence	Reference/Source
Outer Membrane	PMID:9298646	EchoLocation:tolC
outer membrane		From EcoCyc^[3]
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MQMKKLLPIL IGLSLSGFSS LSQAENLMQV YQQARLSNPE LRKSAADRDA AFEKINEARS PLLPQLGLGA DYTYSNGYRD ANGINSNATS ASLQLTQSIF DMSKWRALTL QEKAAGIQDV TYQTDQQTLI LNTATAYFNV LNAIDVLSYT QAQKEAIYRQ LDQTTQRFNV GLVAITDVQN ARAQYDTVLA NEVTARNNLD NAVEQLRQIT GNYYPELAAL NVENFKTDKP QPVNALLKEA EKRNLSLLQA RLSQDLAREQ IRQAQDGHLP TLDLTASTGI SDTSYSGSKT RGAAGTQYDD SNMGQNKVGL SFSLPIYQGG MVNSQVKQAQ YNFVGASEQL ESAHRSVVQT VRSSFNNINA SISSINAYKQ AVVSAQSSLD AMEAGYSVGT RTIVDVLDAT TTLYNAKQEL ANARYNYLIN QLNIKSALGT LNEQDLLALN NALSKPVSTN PENVAPQTPE QNAIADGYAP DSPAPVVQQT SARTTTSNGH NPFRN
Length	495
Mol. Wt	54 kDa
pI	5.4 (calculated)
Extinction coefficient	36,790 (calc based on 21 Y, 1 W, and 0 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
motif	1-22	UniProt Manual:Signal Peptides	UniProt:P02930
Domain	25..209	PF02321 Outer membrane efflux protein		PMID:19920124
Domain	231..427	PF02321 Outer membrane efflux protein		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=tolC taxon=562,83333 </beststructure>

Models

View models at:

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</protect>

Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:90111528	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:947521	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0009965	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P02930	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG11009	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11009	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:947521	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000998	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1002	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli K-12 MC4100	8.85E+02	molecules/cell	Medium: median data from rich and minimal media Temperature (°C): 37 Doubling time (min): Growth Rate(min): Growth phase: other:	emPAI		PMID:18304323
Protein	E. coli K-12 EMG2	120	molecules/cell	Medium: MOPS glucose minimal Temperature (°C): 37 Doubling time (min): Growth phase: Exponential OD: OD600 = 0.2 to 0.25 other: aerated	Quantitative protein sequencing	Spot ID: Ml23	PMID: 9298646
Protein	E. coli K-12 MG1655	8768	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	3141	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	4722	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
tolC
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:3176117..3176157 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

</protect>

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b3035 (EcoliWiki Page)	NCBI GEO profiles for tolC
microarray	GenExpDB:b3035 (EcoliWiki Page)	Summary of data for tolC from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
	GFP Fusion	Intergenic region (3175844..3176155) fused to gfpmut2.	OpenBioSystems	GFP fusion described in Zaslaver, et al. Plate:109-AZ04; Well:H9^[7]
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Notes

Accessions Related to tolC Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11009	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1002	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b3035
EcoGene	EcoGene:EG11009	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000998	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0009965	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Xenopus tropicalis	ENSXETP00000037088 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Shigella flexneri	TOLC	From SHIGELLACYC
E. coli O157	TOLC	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF02321 Outer membrane efflux protein
Pfam (EcoliWiki Page)	PF02321 Outer membrane efflux protein
Superfamily (EcoliWiki Page)	SUPERFAMILY:56954
EcoCyc	EcoCyc:EG11009	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11009	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000998	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1002	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0009965	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 ^1.12 ^1.13 ^1.14 ^1.15 ^1.16 ^1.17 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 ^2.11 ^2.12 ^2.13 ^2.14 ^2.15 ^2.16 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 ^5.3 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 ^6.2 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

tolC:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to tolC Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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