metC:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

metC

Mnemonic

Methionine

Synonyms

ECK3000, b3008, JW2975, ecfA[1]

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

67.9 minutes 

MG1655: 3150258..3151445
<gbrowseImage> name=NC_000913:3150258..3151445 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 3086408..3087595
<gbrowseImage> name=NC_012967:3086408..3087595 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 3037406..3038593
<gbrowseImage> name=NC_012759:3037406..3038593 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 3150892..3152079
<gbrowseImage> name=NC_007779:3150892..3152079 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 3248003..3249190
<gbrowseImage> name=NC_010473:3248003..3249190 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

3150261

Edman degradation

PMID:3513164[2]
PMID:8663055[3]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔmetC (Keio:JW2975)

deletion

deletion

PMID:16738554[4]

Shigen
CGSC10286[5]

metC162::Tn10

Insertion at 3,150,492 bp in MG1655 (NC_000913)

adapted from Nichols et al.[6]

CAG18475 = CGSC7435[5]

Synonyms:

metC69

CGSC:6457

metC162::Tn10

CGSC:7102

metC56

CGSC:9263

metC186

CGSC:9485

metC65

CGSC:23664

metC3158::Tn10kan

PMID:2540407[7]

CGSC:38932

ΔmetC790::kan

PMID:16738554[4]

CGSC:101828


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW2975

Plasmid clone

Shigen

PMID:16769691[8]

Status:Clone OK

Primer 1:GCCGCGGACAAAAAGCTTGATAC

Primer 2:CCTACAATTCGCGCAAAACCGGC

5C10

Kohara Phage

Genobase

PMID:3038334[9]

6H4

Kohara Phage

Genobase

PMID:3038334[9]

nupG511::Tn10

Linked marker

CAG18472 = CGSC7433[5]

est. P1 cotransduction: 13% [6]

tolC210::Tn10

Linked marker

CAG12184 = CGSC7437[5]

est. P1 cotransduction: 34% [6]

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10583

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10583

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000576

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:946240

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0578

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0009878

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. Belfaiza, J et al. (1986) Evolution in biosynthetic pathways: two enzymes catalyzing consecutive steps in methionine biosynthesis originate from a common ancestor and possess a similar regulatory region. Proc. Natl. Acad. Sci. U.S.A. 83 867-71 PubMed
  3. Flint, DH et al. (1996) Studies on the synthesis of the Fe-S cluster of dihydroxy-acid dehydratase in escherichia coli crude extract. Isolation of O-acetylserine sulfhydrylases A and B and beta-cystathionase based on their ability to mobilize sulfur from cysteine and to participate in Fe-S cluster synthesis. J. Biol. Chem. 271 16053-67 PubMed
  4. 4.0 4.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  5. 5.0 5.1 5.2 5.3 CGSC: The Coli Genetics Stock Center
  6. 6.0 6.1 6.2 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  7. Singer, M et al. (1989) A collection of strains containing genetically linked alternating antibiotic resistance elements for genetic mapping of Escherichia coli. Microbiol. Rev. 53 1-24 PubMed
  8. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  9. 9.0 9.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed

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