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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Quickview | Gene | Product(s) | Expression| Evolution | References |

Quickview

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Standard Name

tap

Gene Synonym(s)

ECK1886, b1885, JW1874[1], JW1874

Product Desc.

Tap[2][3];

Component of MCP-IV[2][3]

Dipeptide chemoreceptor, methyl-accepting; MCP IV; flagellar regulon[4]

Product Synonyms(s)

methyl-accepting protein IV[1], B1885[2][1], Tap[2][1] , ECK1886, JW1874, b1885

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): tar-tap-cheRBYZ[2]

Regulation/Activity
Quick Links

porteco.png EcoCyc.gif regulondb.jpg

dnadisplay.png proteindisplay.png   pubmed.jpg   textpresso.jpg  

</protect> See Help:Quickview for help with entering information in the Quickview table. <protect></protect>

Notes

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Tap sense dipeptides indirectly through the periplasmic dipeptide binding protein DppA. Tap is a low-abundance chemoreceptor, 5- 10-fold lower than Tar and Tsr. Tap can function as a cold receptor during thermotaxis. Tap senses low pH as a repellent.[4]



Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

tap

Mnemonic

Taxis protein

Synonyms

ECK1886, b1885, JW1874[1], JW1874

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

42.4 minutes 

MG1655: 1969008..1967407
<gbrowseImage> name=NC_000913:1967407..1969008 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 1949754..1948153
<gbrowseImage> name=NC_012967:1948153..1949754 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 1859466..1861067
<gbrowseImage> name=NC_012759:1859466..1861067 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 1972698..1971097
<gbrowseImage> name=NC_007779:1971097..1972698 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 2059579..2057978
<gbrowseImage> name=NC_010473:2057978..2059579 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

Δtap (Keio:JW1874)

deletion

deletion

PMID:16738554

Shigen
CGSC9560[5]

tap::Tn5KAN-2 (FB20489)

Insertion at nt 194 in Minus orientation

PMID:15262929

E. coli Genome Project:FB20489

does not contain pKD46

tap::Tn5KAN-2 (FB20490)

Insertion at nt 194 in Minus orientation

PMID:15262929

E. coli Genome Project:FB20490

contains pKD46

Δtap::kan

deletion

Biolog:respiration

unable to respire Acetate

PMID:16095938

Δtap::kan

deletion

Biolog:respiration

unable to respire a-Ketoglutarate

PMID:16095938

Δtap::kan

deletion

Biolog:respiration

unable to respire L-Aspartate

PMID:16095938

Δtap-738::kan

PMID:16738554

CGSC:103086


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Notes

Genetic Interactions

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<protect>

Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW1874

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCTTTAATCGTATTCGAATTTC

Primer 2:CCGGATACCACTGGCGCAATTTG

12C7

Kohara Phage

Genobase

PMID:3038334

7F11

Kohara Phage

Genobase

PMID:3038334

eda-51::Tn10

Linked marker

CAG18486 = CGSC7392[5]

est. P1 cotransduction: 21% [6]

uvrC279::Tn10

Linked marker

CAG12156 = CGSC7394[5]

est. P1 cotransduction: 43% [6]

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10987

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10987

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000976

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:946397

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0980

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0006288

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

Tap

Synonyms

methyl-accepting protein IV[1], B1885[2][1], Tap[2][1] , ECK1886, JW1874, b1885

Product description

Tap[2][3];

Component of MCP-IV[2][3]

Dipeptide chemoreceptor, methyl-accepting; MCP IV; flagellar regulon[4]

EC number (for enzymes)


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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status

GO:0004871

signal transducer activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR003660

F

Seeded from EcoCyc (v14.0)

complete

GO:0004871

signal transducer activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004089

F

Seeded from EcoCyc (v14.0)

complete

GO:0004871

signal transducer activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004090

F

Seeded from EcoCyc (v14.0)

complete

GO:0004871

signal transducer activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004091

F

Seeded from EcoCyc (v14.0)

complete

GO:0004871

signal transducer activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0807

F

Seeded from EcoCyc (v14.0)

complete

GO:0004888

transmembrane receptor activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR003122

F

Seeded from EcoCyc (v14.0)

complete

GO:0005886

plasma membrane

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0997

C

Seeded from EcoCyc (v14.0)

complete

GO:0005886

plasma membrane

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-1003

C

Seeded from EcoCyc (v14.0)

complete

GO:0006935

chemotaxis

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR003122

P

Seeded from EcoCyc (v14.0)

complete

GO:0006935

chemotaxis

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004089

P

Seeded from EcoCyc (v14.0)

complete

GO:0006935

chemotaxis

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004090

P

Seeded from EcoCyc (v14.0)

complete

GO:0006935

chemotaxis

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004091

P

Seeded from EcoCyc (v14.0)

complete

GO:0006935

chemotaxis

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0145

P

Seeded from EcoCyc (v14.0)

complete

GO:0007165

signal transduction

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR003122

P

Seeded from EcoCyc (v14.0)

complete

GO:0007165

signal transduction

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR003660

P

Seeded from EcoCyc (v14.0)

complete

GO:0007165

signal transduction

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004089

P

Seeded from EcoCyc (v14.0)

complete

GO:0007165

signal transduction

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004090

P

Seeded from EcoCyc (v14.0)

complete

GO:0007165

signal transduction

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004091

P

Seeded from EcoCyc (v14.0)

complete

GO:0007165

signal transduction

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0807

P

Seeded from EcoCyc (v14.0)

complete

GO:0016020

membrane

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR003122

C

Seeded from EcoCyc (v14.0)

complete

GO:0016020

membrane

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004089

C

Seeded from EcoCyc (v14.0)

complete

GO:0016020

membrane

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004090

C

Seeded from EcoCyc (v14.0)

complete

GO:0016020

membrane

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004091

C

Seeded from EcoCyc (v14.0)

complete

GO:0016020

membrane

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0472

C

Seeded from EcoCyc (v14.0)

complete

GO:0016021

integral to membrane

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR003660

C

Seeded from EcoCyc (v14.0)

complete

GO:0016021

integral to membrane

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0812

C

Seeded from EcoCyc (v14.0)

complete

GO:0016021

integral to membrane

GO_REF:0000023

IEA: Inferred from Electronic Annotation

SP_SL:SL-9909

C

Seeded from EcoCyc (v14.0)

complete

Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

Subunits of MCP-IV

could be indirect


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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes

plasma membrane

C-terminus localized in the cytoplasm with 2 predicted transmembrane domains

Daley et al. (2005) [7]

plasma membrane

From EcoCyc[3]


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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc

MFNRIRISTT LFLILILCGI LQIGSNGMSF WAFRDDLQRL NQVEQSNQQR AALAQTRAVM
LQASTALNKA GTLTALSYPA DDIKTLMTTA RASLTQSTTL FKSFMAMTAG NEHVRGLQKE
TEKSFARWHN DLEHQATWLE SNQLSDFLTA PVQGSQNAFD VNFEAWQLEI NHVLEAASAQ
SQRNYQISAL VFISMIIVAA IYISSALWWT RKMIVQPLAI IGSHFDSIAA GNLARPIAVY
GRNEITAIFA SLKTMQQALR GTVSDVRKGS QEMHIGIAEI VAGNNDLSSR TEQQAASLAQ
TAASMEQLTA TVGQNADNAR QASELAKNAA TTAQAGGVQV STMTHTMQEI ATSSQKIGDI
ISVIDGIAFQ TNILALNAAV EAARAGEQGR GFAVVAGEVR NLASRSAQAA KEIKGLIEES
VNRVQQGSKL VNNAAATMID IVSSVTRVND IMGEIASASE EQQRGIEQVA QAVSQMDQVT
QQNASLVEEA AVATEQLANQ ADHLSSRVAV FTLEEHEVAR HESVQLQIAP VVS
Length

533

Mol. Wt

57.511 kDa

pI

5.6 (calculated)

Extinction coefficient

38,960 - 39,085 (calc based on 4 Y, 6 W, and 1 C residues)


Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

192..261

PF00672 HAMP domain

PMID:19920124

Domain

314..514

PF00015 Methyl-accepting chemotaxis protein (MCP) signaling domain

PMID:19920124

Domain

44..177

PF02203 Tar ligand binding domain homologue

PMID:19920124

<motif_map/>

tmhmm.php?gene=tap&show=pic&.jpg
Click the image to view raw output from TMHMM.

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=tap taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16129837

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:946397

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0006288

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P07018

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10987

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10987

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:946397

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000976

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0980

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

Protein

E. coli K-12 MG1655

0a

molecules/cell/generation

  • Medium: MOPS Complete
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

Low confidence in the sequencing data set.

PMID: 24766808

Protein

E. coli K-12 MG1655

2a

molecules/cell/generation

  • Medium: MOPS Minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

Low confidence in the sequencing data set.

PMID: 24766808

Protein

E. coli K-12 MG1655

0a

molecules/cell/generation

  • Medium: MOPS Complete without Methionine
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

Low confidence in the sequencing data set.

PMID: 24766808

Notes

Transcription and Transcriptional Regulation

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<protect>

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

tar-tap-cheRBYZ

Figure courtesy of RegulonDB

</protect>

Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:1968988..1969028 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference


Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GEO Profiles:b1885 (EcoliWiki Page)

NCBI GEO profiles for tap

microarray

GenExpDB:b1885 (EcoliWiki Page)

Summary of data for tap from multiple microarray studies


Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes

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Notes

Accessions Related to tap Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10987

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0980

Escherichia coli str. K-12 substr. MG1655

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b1885

EcoGene

EcoGene:EG10987

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000976

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0006288

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism Homologs (Statistics) Comments

Anopheles gambiae

  • ENSANGP00000025965 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Xenopus tropicalis

  • ENSXETP00000015274 (score: 1.000; bootstrap: 51%)
  • ENSXETP00000018487 (score: 1.000; bootstrap: 51%)

From Inparanoid:20070104

Shigella flexneri

TAP

From SHIGELLACYC

E. coli O157

TAP

From ECOO157CYC


Do-It-Yourself Web Tools

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Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

Pfam (EcoliWiki Page)

PF00015 Methyl-accepting chemotaxis protein (MCP) signaling domain

Pfam (EcoliWiki Page)

PF00672 HAMP domain

Superfamily (EcoliWiki Page)

SUPERFAMILY:47170

Superfamily (EcoliWiki Page)

SUPERFAMILY:58104

Pfam (EcoliWiki Page)

PF02203 Tar ligand binding domain homologue

EcoCyc

EcoCyc:EG10987

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10987

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000976

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0980

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0006288

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.0 2.1 2.2 2.3 2.4 2.5 2.6 2.7 2.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. 3.0 3.1 3.2 3.3 3.4 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. 4.0 4.1 4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
  6. 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  7. Daley, DO et al. (2005) Global topology analysis of the Escherichia coli inner membrane proteome. Science 308 1321-3 PubMed

Categories

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