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Gene |

Product(s) |

Expression|

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Standard Name	nupG
Gene Synonym(s)	ECK2959, b2964, JW2932^[1], JW2932, gru^[2]
Product Desc.	NupG nucleoside MFS transporter^[3]^[4] Nucleoside transporter^[5]
Product Synonyms(s)	nucleoside transporter^[1], B2964^[3]^[1], NupG^[3]^[1] , ECK2959, JW2932, b2964
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): yggX-mltC-nupG^[3], mutY-yggX-mltC-nupG^[3], nupG^[3], OP00172
Regulation/Activity
Quick Links
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Notes

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NupG, XapB and YegT are paralogs. Major Facilitator Superfamily (MFS). CytR regulon. DeoR regulon.^[5]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	nupG
Mnemonic	Nucleoside permease
Synonyms	ECK2959, b2964, JW2932^[1], JW2932, gru^[2]
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	66.9 minutes	MG1655: 3103736..3104992 <gbrowseImage> name=NC_000913:3103736..3104992 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 2991375..2992631 <gbrowseImage> name=NC_012967:2991375..2992631 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 2990884..2992140 <gbrowseImage> name=NC_012759:2990884..2992140 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 3104370..3105626 <gbrowseImage> name=NC_007779:3104370..3105626 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Type	Phenotype: Description	Reference	Availability	Comments
nupG(del) (Keio:JW2932)	deletion	deletion			PMID:16738554	Shigen CGSC10267^[6]
nupG::Tn5KAN-I-SceI (FB20994)	Insertion at nt 723 in Minus orientation				PMID:15262929	E. coli Genome Project:FB20994	contains pKD46
nupG::Tn5KAN-I-SceI (FB20995)	Insertion at nt 723 in Minus orientation				PMID:15262929	E. coli Genome Project:FB20995	does not contain pKD46
nupG511::Tn10	Insertion at 3,104,399 bp in MG1655 (NC_000913)				adapted from Nichols et al.^[7]	CAG18472 = CGSC7433^[6]	Synonyms:
nupG511::Tn10						CGSC:8684
nupG3157::Tn10kan					PMID:2540407	CGSC:40115
nupG739(del)::kan					PMID:16738554	CGSC:102166
gru⁺			Resistant to	resistant to showdomycin	PMID:827549	SO736
nupC ^- nupG^-			Resistant to	Fluorouracil resistance Fluoruridine resistance Fluorodeoxycytidine resistance	PMID:827549		figure 1
nupG in strain S0 1131			Resistant to	Resistance to Fluorodeoxyuridine	PMID:374403	S0 1131
nupG nupC^-			Resistant to	Resistant to showdomycin	PMID:374403	S0 1131	Figure 5
nupG in strain S0 861			Resistant to	Resistance to 5-fluoro 2'deoxycytidine	PMID:374403	S0 861	2A
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Notes

Mutants containing the nupG system were obtained by mating and recombination in a transport negative strain, S_Φ587. ^[2] The nupG system determines high-affinity nucleoside transport across the cell membrane. nupG⁺ results in nucleoside transport and these imported nucleosides serve as antecedents of DNA and RNA. nupG^- is a mutant and does not allow nucleoside transport across the membrane. ^[8]

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW2932	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCAATCTTAAGCTGCAGCTGAA Primer 2:CCGTGGCTAACCGTCTGTGTGCC
3A9	Kohara Phage	Genobase	PMID:3038334
speA210::Tn10	Linked marker	CAG12168 = CGSC7431^[6]	est. P1 cotransduction: 52% ^[7] Synonyms:zgd-210::Tn10, zgf-210::Tn10
metC162::Tn10	Linked marker	CAG18475 = CGSC7435^[6]	est. P1 cotransduction: 12% ^[7]
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10664	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10664	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000657	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:946282	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0658	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0009728	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	NupG
Synonyms	nucleoside transporter^[1], B2964^[3]^[1], NupG^[3]^[1] , ECK2959, JW2932, b2964
Product description	NupG nucleoside MFS transporter^[3]^[4] Nucleoside transporter^[5]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0005337	nucleoside transmembrane transporter activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR004740	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005886	plasma membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0997	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005886	plasma membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-1003	C	Seeded from EcoCyc (v14.0)	complete
	GO:0006810	transport	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0813	P	Seeded from EcoCyc (v14.0)	complete
	GO:0015858	nucleoside transport	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR004740	P	Seeded from EcoCyc (v14.0)	complete
	GO:0005887	integral to plasma membrane	PMID:15513740	IDA: Inferred from Direct Assay		C		complete
	GO:0016020	membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0472	C	Seeded from EcoCyc (v14.0)	complete
	GO:0015864	pyrimidine nucleoside transport	PMID:15513740	IDA: Inferred from Direct Assay		P		complete
	GO:0016021	integral to membrane	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR004740	C	Seeded from EcoCyc (v14.0)	complete
	GO:0015862	uridine transport	PMID:15513740	IDA: Inferred from Direct Assay		P		complete
	GO:0016021	integral to membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0812	C	Seeded from EcoCyc (v14.0)	complete
	GO:0015213	uridine transmembrane transporter activity	PMID:15513740	IDA: Inferred from Direct Assay		F		complete
	GO:0016021	integral to membrane	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-9909	C	Seeded from EcoCyc (v14.0)	complete
	GO:0015214	pyrimidine nucleoside transmembrane transporter activity	PMID:15513740	IDA: Inferred from Direct Assay		F		complete
	GO:0015213	uridine transmembrane transporter activity	PMID:374403	IMP: Inferred from Mutant Phenotype		F	Fig. 3	complete
	GO:0015212	cytidine transmembrane transporter activity	PMID:374403	IMP: Inferred from Mutant Phenotype		F	Fig. 3	complete
	GO:0032238	adenosine transport	PMID:374403	IMP: Inferred from Mutant Phenotype		P	Fig. 3	complete
	GO:0015860	purine nucleoside transmembrane transport	PMID:374403	IMP: Inferred from Mutant Phenotype		P	Fig. 3, both guanosine and adenosine are transported	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source
plasma membrane	C-terminus localized in the cytoplasm with 12 predicted transmembrane domains	Daley et al. (2005) ^[9]
plasma membrane			From EcoCyc^[4]
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MCFFKHSSAS RCEEINMNLK LQLKILSFLQ FCLWGSWLTT LGSYMFVTLK FDGASIGAVY SSLGIAAVFM PALLGIVADK WLSAKWVYAI CHTIGAITLF MAAQVTTPEA MFLVILINSF AYMPTLGLIN TISYYRLQNA GMDIVTDFPP IRIWGTIGFI MAMWVVSLSG FELSHMQLYI GAALSAILVL FTLTLPHIPV AKQQANQSWT TLLGLDAFAL FKNKRMAIFF IFSMLLGAEL QITNMFGNTF LHSFDKDPMF ASSFIVQHAS IIMSISQISE TLFILTIPFF LSRYGIKNVM MISIVAWILR FALFAYGDPT PFGTVLLVLS MIVYGCAFDF FNISGSVFVE KEVSPAIRAS AQGMFLMMTN GFGCILGGIV SGKVVEMYTQ NGITDWQTVW LIFAGYSVVL AFAFMAMFKY KHVRVPTGTQ TVSH
Length	434
Mol. Wt	48.262 kDa
pI	8.6 (calculated)
Extinction coefficient	74,370 - 75,120 (calc based on 13 Y, 10 W, and 6 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	1..407	PF03825 Nucleoside H+ symporter		PMID:19920124
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<motif_map/>

Click the image to view raw output from TMHMM.

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=nupG taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:90111521	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:946282	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0009728	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P0AFF4	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG10664	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10664	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:946282	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000657	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0658	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Reference(s)
Protein	E. coli K-12 MG1655	230	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	49	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	30	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
yggX-mltC-nupG
mutY-yggX-mltC-nupG
nupG
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:3103716..3103756 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b2964 (EcoliWiki Page)	NCBI GEO profiles for nupG
microarray	GenExpDB:b2964 (EcoliWiki Page)	Summary of data for nupG from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
	GFP Fusion	Intergenic region (3103439..3103701) fused to gfpmut2.	OpenBioSystems	GFP fusion described in Zaslaver, et al. Plate:109-AZ16; Well:E8^[10]
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Notes

Accessions Related to nupG Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10664	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0658	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b2964
EcoGene	EcoGene:EG10664	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000657	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0009728	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Anopheles gambiae	ENSANGP00000010355 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Drosophila melanogaster	FBgn0036191 (score: 1.000; bootstrap: 100%) FBgn0034093 (score: 0.149)	From Inparanoid:20070104
Shigella flexneri	NUPG	From SHIGELLACYC
E. coli O157	NUPG	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF03825 Nucleoside H+ symporter
Superfamily (EcoliWiki Page)	SUPERFAMILY:103473
EcoCyc	EcoCyc:EG10664	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10664	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000657	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0658	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0009728	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 Munch-Petersen, A & Mygind, B (1976) Nucleoside transport systems in Escherichia coli K12: specificity and regulation. J. Cell. Physiol. 89 551-9 PubMed
↑ ^3.0 ^3.1 ^3.2 ^3.3 ^3.4 ^3.5 ^3.6 ^3.7 ^3.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^5.0 ^5.1 ^5.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^6.0 ^6.1 ^6.2 ^6.3 CGSC: The Coli Genetics Stock Center
↑ ^7.0 ^7.1 ^7.2 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Westh Hansen, SE et al. (1987) Studies on the sequence and structure of the Escherichia coli K-12 nupG gene, encoding a nucleoside-transport system. Eur. J. Biochem. 168 385-91 PubMed
↑ Daley, DO et al. (2005) Global topology analysis of the Escherichia coli inner membrane proteome. Science 308 1321-3 PubMed
↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

nupG:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to nupG Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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