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Standard Name	mhpB
Gene Synonym(s)	ECK0345, b0348, JW0339^[1], JW0339
Product Desc.	MhpB^[2]^[3]; Component of 3-(2,3-dihydroxyphenyl)propionate dioxygenase^[2]^[3]
Product Synonyms(s)	2,3-dihydroxyphenylpropionate 1,2-dioxygenase^[1], B0348^[2]^[1], MhpB^[2]^[1] , ECK0345, JW0339, b0348
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): mhpABCDFE^[2]
Regulation/Activity
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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	mhpB
Mnemonic	m-Hydroxyphenylpropionic acid
Synonyms	ECK0345, b0348, JW0339^[1], JW0339
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	7.96 minutes	MG1655: 369501..370445 <gbrowseImage> name=NC_000913:369501..370445 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 342833..343777 <gbrowseImage> name=NC_012967:342833..343777 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
W3110		W3110: 369501..370445 <gbrowseImage> name=NC_007779:369501..370445 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 1388944..1389888 <gbrowseImage> name=NC_010473:1388944..1389888 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
Coding Start (SO:0000323)	MG1655	369501	Edman degradation	PMID:8399388
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Description	Reference	Availability	Comments
mhpB(del) (Keio:JW0339)	deletion	deletion		PMID:16738554	Shigen
mhpBD114N		D114N	Low level of catalytic activity, 600-fold lower than the wild-type enzyme. More than 8000-fold decrease in affinity			seeded from UniProt:P0ABR9
mhpBD114A		D114A	Complete loss of extradiol cleavage activity			seeded from UniProt:P0ABR9
mhpBP181H		P181H	More than 60-fold decrease in catalytic activity and affinity			seeded from UniProt:P0ABR9
mhpBH115A		H115A	Complete loss of extradiol cleavage activity			seeded from UniProt:P0ABR9
mhpBH115Q		H115Q	Complete loss of activity			seeded from UniProt:P0ABR9
mhpBH115Y		H115Y	Complete loss of activity			seeded from UniProt:P0ABR9
mhpBH179A		H179A	Complete loss of activity			seeded from UniProt:P0ABR9
mhpBH179Q		H179Q	Complete loss of activity			seeded from UniProt:P0ABR9
mhpBH179Y		H179Y	Complete loss of activity			seeded from UniProt:P0ABR9
mhpBP181A		P181A	More than 2-fold decrease in catalytic activity and 100-fold decrease in affinity			seeded from UniProt:P0ABR9
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW0339	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCCACGCTTATCTTCACTGTCT Primer 2:CCGTTCTCTGTTCTGGCGCTTAA
lacI3042::Tn10	Linked marker	CAG18439 = CGSC7334^[4]	est. P1 cotransduction: 91% ^[5] Synonyms:lacI42::Tn10 nnnCAG18439 also carries lacZ118(0c) (CGSC).
mhpC281::Tn10	Linked marker	CAG12080 = CGSC7333^[4]	est. P1 cotransduction: 95% ^[5] Synonyms:zah-281::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:M011	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG20274	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120004470	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:945047	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB4167	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0001198	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	MhpB
Synonyms	2,3-dihydroxyphenylpropionate 1,2-dioxygenase^[1], B0348^[2]^[1], MhpB^[2]^[1] , ECK0345, JW0339, b0348
Product description	MhpB^[2]^[3]; Component of 3-(2,3-dihydroxyphenyl)propionate dioxygenase^[2]^[3]
EC number (for enzymes)	1.13.11.-^[1]
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0005737	cytoplasm				C	Seeded from Riley et al 2006 ^[1].	Missing: evidence, reference
	GO:0005506	iron ion binding	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_01653	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005506	iron ion binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0408	F	Seeded from EcoCyc (v14.0)	complete
	GO:0006725	cellular aromatic compound metabolic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR004183	P	Seeded from EcoCyc (v14.0)	complete
	GO:0008198	ferrous iron binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR004183	F	Seeded from EcoCyc (v14.0)	complete
	GO:0008669	2,3-dihydroxy-phenylpropionate 1,2-dioxygenase activity	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_01653	F	Seeded from EcoCyc (v14.0)	complete
	GO:0016491	oxidoreductase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR004183	F	Seeded from EcoCyc (v14.0)	complete
	GO:0016491	oxidoreductase activity	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0560	F	Seeded from EcoCyc (v14.0)	complete
	GO:0016702	oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0223	F	Seeded from EcoCyc (v14.0)	complete
	GO:0019439	aromatic compound catabolic process	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_01653	P	Seeded from EcoCyc (v14.0)	complete
	GO:0019439	aromatic compound catabolic process	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0058	P	Seeded from EcoCyc (v14.0)	complete
	GO:0047070	3-carboxyethylcatechol 2,3-dioxygenase activity	GOA:spec GO_REF:0000003	IEA: Inferred from Electronic Annotation	EC:1.13.11.16	F	Seeded from EcoCyc (v14.0)	complete
	GO:0055114	oxidation reduction	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0560	P	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	Subunits of 3-(2,3-dihydroxyphenyl)propionate dioxygenase	could be indirect
Protein	glpD		PMID:16606699	Experiment(s):EBI-1136321
Protein	nadE		PMID:16606699	Experiment(s):EBI-1136321
Protein	helD		PMID:16606699	Experiment(s):EBI-1136321
Protein	groL		PMID:16606699	Experiment(s):EBI-1136321
Protein	ldcC		PMID:16606699	Experiment(s):EBI-1136321
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MHAYLHCLSH SPLVGYVDPA QEVLDEVNGV IASARERIAA FSPELVVLFA PDHYNGFFYD VMPPFCLGVG ATAIGDFGSA AGELPVPVEL AEACAHAVMK SGIDLAVSYC MQVDHGFAQP LEFLLGGLDK VPVLPVFING VATPLPGFQR TRMLGEAIGR FTSTLNKRVL FLGSGGLSHQ PPVPELAKAD AHMRDRLLGS GKDLPASERE LRQQRVISAA EKFVEDQRTL HPLNPIWDNQ FMTLLEQGRI QELDAVSNEE LSAIAGKSTH EIKTWVAAFA AISAFGNWRS EGRYYRPIPE WIAGFGSLSA RTEN
Length	314
Mol. Wt	34.195 kDa
pI	5.5 (calculated)
Extinction coefficient	32,430 - 32,930 (calc based on 7 Y, 4 W, and 4 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	4..306	PF02900 Catalytic LigB subunit of aromatic ring-opening dioxygenase		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=mhpB taxon=562,83333 </beststructure>

Models

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16128333	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:945047	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0001198	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P0ABR9	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:M011	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG20274	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:945047	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120004470	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB4167	Escherichia coli str. K-12 substr. MG1655
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Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli K-12 MG1655	1^a	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
Protein	E. coli K-12 MG1655	3^a	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
Protein	E. coli K-12 MG1655	0^a	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
mhpABCDFE
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:369481..369521 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b0348 (EcoliWiki Page)	NCBI GEO profiles for mhpB
microarray	GenExpDB:b0348 (EcoliWiki Page)	Summary of data for mhpB from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to mhpB Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:M011	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB4167	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b0348
EcoGene	EcoGene:EG20274	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120004470	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0001198	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Xenopus tropicalis	ENSXETP00000013953 (score: 1.000; bootstrap: 100%) ENSXETP00000013497 (score: 0.187)	From Inparanoid:20070104
E. coli O157	MHPB	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF02900 Catalytic LigB subunit of aromatic ring-opening dioxygenase
Superfamily (EcoliWiki Page)	SUPERFAMILY:53213
EcoCyc	EcoCyc:M011	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG20274	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120004470	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB4167	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0001198	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 ^1.8 ^1.9 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 ^2.7 ^2.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 ^3.3 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 CGSC: The Coli Genetics Stock Center
↑ ^5.0 ^5.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

mhpB:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to mhpB Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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