mdh:On One Page

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Quickview | Gene | Product(s) | Expression| Evolution | References |

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Standard Name

mdh

Gene Synonym(s)

ECK3225, b3236, JW3205[1], JW3205

Product Desc.

Mdh[2][3];

Component of malate dehydrogenase[2][3]

Malate dehydrogenase, NAD-dependent[4]

Product Synonyms(s)

malate dehydrogenase, NAD(P)-binding[1], B3236[2][1], Mdh[2][1] , ECK3225, JW3205, b3236

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): mdh[2], OP00149

Regulation/Activity
Quick Links

porteco.png EcoCyc.gif regulondb.jpg

dnadisplay.png proteindisplay.png   pubmed.jpg   textpresso.jpg  

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Notes

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Mdh is elevated 4X during growth at 4C (enabled by Oleispira antarctica Cpn10/60 transgene). Deletion of mdh induces growth defects on all substrates except glucose. After four hours of Zn(II) stress, protein levels increased (Easton, 2006).[4]



Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

mdh

Mnemonic

Malate DeHydrogenase

Synonyms

ECK3225, b3236, JW3205[1], JW3205

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

72.88 minutes 

MG1655: 3382290..3381352
<gbrowseImage> name=NC_000913:3381352..3382290 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 3312165..3311227
<gbrowseImage> name=NC_012967:3311227..3312165 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 3268500..3269438
<gbrowseImage> name=NC_012759:3268500..3269438 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 3384123..3383185
<gbrowseImage> name=NC_007779:3383185..3384123 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 3480035..3479097
<gbrowseImage> name=NC_010473:3479097..3480035 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

3381352

Edman degradation

PMID:7028159
PMID:8506346
PMID:8670822
PMID:9298646
PMID:9600841
PMID:9740056


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

mdh(del) (Keio:JW3205)

deletion

deletion

PMID:16738554

Shigen
CGSC10430[5]

mdh::Tn5KAN-I-SceI (FB21080)

Insertion at nt 659 in Plus orientation

PMID:15262929

E. coli Genome Project:FB21080

contains pKD46

mdh::Tn5KAN-I-SceI (FB21081)

Insertion at nt 659 in Plus orientation

PMID:15262929

E. coli Genome Project:FB21081

does not contain pKD46

mdhA291S

A291S

(in strain: EC35)

Strain variation; seeded from UniProt:P61889

mdhG294A

G294A

(in strain: ECOR 45)

Strain variation; seeded from UniProt:P61889

mdhD297N

D297N

(in strain: E830587)

Strain variation; seeded from UniProt:P61889

mdhR153C

R153C

Loss of interaction with substrate

seeded from UniProt:P61889

mdhA232T

A232T

(in strain: ECO R37)

Strain variation; seeded from UniProt:P61889

mdhV249I

V249I

(in strain: RT083)

Strain variation; seeded from UniProt:P61889

mdhQ289K

Q289K

(in strain: EC35, EC38, EC40, EC44, EC46, EC47 and RT272)

Strain variation; seeded from UniProt:P61889

mdhN290S

N290S

(in strain: E2666-74, ECOR 27, ECOR 45, RL012A, RT104 and RT174)

Strain variation; seeded from UniProt:P61889

mdhA209P

A209P

(in strain: MB001D)

Strain variation; seeded from UniProt:P61889

mdhD71N

D71N

(in strain: EC47, EC49, EC50 and RT272)

Strain variation; seeded from UniProt:P61889

mdhA106S

A106S

(in strain: ECOR 27 and RT082)

Strain variation; seeded from UniProt:P61889

mdhA218R

A218R

(in strain: A8190, E2666-74, E830587, E851819, E3406, EC10, EC14, EC32, EC35, EC38, EC40, EC44, EC46, EC47, EC49, EC50, EC52, EC58, E64 and EC70)

Strain variation; seeded from UniProt:P61889

mdh(del)::kan

deletion

Biolog:respiration

unable to respire D-Galacturonate

PMID:16095938

mdh(del)::kan

deletion

Biolog:respiration

unable to respire Acetate

PMID:16095938

mdh(del)::kan

deletion

Biolog:respiration

unable to respire a-Hydroxybutyrate

PMID:16095938

mdh(del)::kan

deletion

Biolog:respiration

unable to respire a-Ketobutyrate

PMID:16095938

mdh(del)::kan

deletion

Biolog:respiration

unable to respire a-Ketoglutarate

PMID:16095938

mdh(del)::kan

deletion

Biolog:respiration

unable to respire DL-Lactate

PMID:16095938

mdh(del)::kan

deletion

Biolog:respiration

unable to respire Succinate

PMID:16095938

mdh(del)::kan

deletion

Biolog:respiration

unable to respire Bromosuccinate

PMID:16095938

mdh(del)::kan

deletion

Biolog:respiration

unable to respire Glucuronamide

PMID:16095938

mdh(del)::kan

deletion

Biolog:respiration

unable to respire L-Alanine

PMID:16095938

mdh(del)::kan

deletion

Biolog:respiration

unable to respire L-Alanyl-glycine

PMID:16095938

mdh(del)::kan

deletion

Biolog:respiration

unable to respire L-Asparagine

PMID:16095938

mdh(del)::kan

deletion

Biolog:respiration

unable to respire L-Aspartate

PMID:16095938

mdh(del)::kan

deletion

Biolog:respiration

unable to respire L-Serine

PMID:16095938

mdh-10

CGSC:12020

mdh-2

CGSC:12028

mdh-0

CGSC:15729

mdh-761(del)::kan

PMID:16738554

CGSC:101774

mdh761(del)::FRTKanFRT

deletion

Mutagenesis Rate

Decrease in Stress Induced Mutagenesis (SIM).

PMID:23224554

Parent Stain: SMR4562 Experimental Stain: SMR12060

The mutation conferred a medium decrease in SIM with mutant frequency reduced by roughly 67 to 89 percent of the Wild type. See table S3 for full experimental data.

mdh761(del)::FRTKanFRT

deletion

Sensitivity to

SDS-EDTA Sensitivity

PMID:23224554

Parent Stain: SMR4562 Experimental Stain: SMR12060

The mutation conferred an increase in SDS-EDTA sensitivity. See table S7 and S1 for summarized data.

SMR4562 mdh761(del)::FRTKanFRT yiaG-yfp FRTcatFRT

deletion

Sigma S activity

Decrease in SigmaS activity.

PMID:23224554

Parental Strain: SMR10582 Experimental Strain: SMR13058

See table S8 for full experimental results.

CAG45114 mdh761(del)::FRTKanFRT

Deletion

Sigma E activity

Decrease in sigmaE activity

PMID:23224554

Parental Strain: CAG45114 Experimental Strain: SMR15356

See table S11 for full experimental data.

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Notes

Genetic Interactions

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<protect>

Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW3205

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCAAAGTCGCAGTCCTCGGCGC

Primer 2:CCtTTATTAACGAACTCTTCGCC

1E3

Kohara Phage

Genobase

PMID:3038334

3G9

Kohara Phage

Genobase

PMID:3038334

zhc-6::Tn10

Linked marker

CAG12153 = CGSC7442[5]

est. P1 cotransduction: 53% [6]
Synonyms:zha-6::Tn10

acrF3083::Tn10

Linked marker

CAG12075 = CGSC7447[5]

est. P1 cotransduction: 26% [6]
Synonyms:zhd-3083::Tn10, zhe-3083::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10576

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10576

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000569

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:947854

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0571

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0010613

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

Mdh

Synonyms

malate dehydrogenase, NAD(P)-binding[1], B3236[2][1], Mdh[2][1] , ECK3225, JW3205, b3236

Product description

Mdh[2][3];

Component of malate dehydrogenase[2][3]

Malate dehydrogenase, NAD-dependent[4]

EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status

GO:0005737

cytoplasm

C

Seeded from Riley et al 2006 [1].

Missing: evidence, reference

GO:0016615

malate dehydrogenase activity

PMID:4914076

IMP: Inferred from Mutant Phenotype

F

complete

GO:0000300

peripheral to membrane of membrane fraction

PMID:15976458

IDA: Inferred from Direct Assay

C

Seeded from EcoCyc (v14.0)

complete

GO:0005488

binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR016040

F

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

PMID:4914076

IDA: Inferred from Direct Assay

C

Seeded from EcoCyc (v14.0)

complete

GO:0005829

cytosol

PMID:16858726

IDA: Inferred from Direct Assay

C

Seeded from EcoCyc (v14.0)

complete

GO:0005975

carbohydrate metabolic process

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR015955

P

Seeded from EcoCyc (v14.0)

complete

GO:0006096

glycolysis

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001557

P

Seeded from EcoCyc (v14.0)

complete

GO:0016615

malate dehydrogenase activity

PMID:4960671

IDA: Inferred from Direct Assay

F

complete

GO:0006099

tricarboxylic acid cycle

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_01516

P

Seeded from EcoCyc (v14.0)

complete

GO:0016491

oxidoreductase activity

PMID:4960671

IDA: Inferred from Direct Assay

F

complete

GO:0006099

tricarboxylic acid cycle

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0816

P

Seeded from EcoCyc (v14.0)

complete

GO:0006108

malate metabolic process

PMID:4960671

IDA: Inferred from Direct Assay

P

complete

GO:0006108

malate metabolic process

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001252

P

Seeded from EcoCyc (v14.0)

complete

GO:0006108

malate metabolic process

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR010097

P

Seeded from EcoCyc (v14.0)

complete

GO:0009061

anaerobic respiration

PMID:2993232

IDA: Inferred from Direct Assay

P

complete

GO:0016020

membrane

PMID:16858726

IDA: Inferred from Direct Assay

C

Seeded from EcoCyc (v14.0)

complete

GO:0016615

malate dehydrogenase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001252

F

Seeded from EcoCyc (v14.0)

complete

GO:0006113

fermentation

PMID:2993232

IDA: Inferred from Direct Assay

P

complete

GO:0016616

oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001557

F

Seeded from EcoCyc (v14.0)

complete

GO:0006099

tricarboxylic acid cycle

PMID:2993232

IDA: Inferred from Direct Assay

P

complete

GO:0016615

malate dehydrogenase activity

PMID:2993232

IDA: Inferred from Direct Assay

F

complete

GO:0030060

L-malate dehydrogenase activity

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_01516

F

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

PMID:4914076

IDA: Inferred from Direct Assay

C

The authors conclude that 96% of malate dehydrogenase (Mdh) activity was present in the soluble fraction of mechanically disrupted cells subjected to ultracentrifugation while 4% was in the membrane fraction (cells were grown in minimal malate medium) (pp 725).

complete

GO:0006096

glycolysis

PMID:2993232

IDA: Inferred from Direct Assay

P

complete

GO:0030060

L-malate dehydrogenase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR010097

F

Seeded from EcoCyc (v14.0)

complete

GO:0000300

peripheral to membrane of membrane fraction

PMID:15976458

IDA: Inferred from Direct Assay

C

The authors demonstrate the presence of Mdh in the membrane fraction (Figure 3).

complete

GO:0030060

L-malate dehydrogenase activity

GOA:spec
GO_REF:0000003

IEA: Inferred from Electronic Annotation

EC:1.1.1.37

F

Seeded from EcoCyc (v14.0)

complete

GO:0055114

oxidation reduction

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001236

P

Seeded from EcoCyc (v14.0)

complete

GO:0055114

oxidation reduction

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001252

P

Seeded from EcoCyc (v14.0)

complete

GO:0055114

oxidation reduction

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001557

P

Seeded from EcoCyc (v14.0)

complete

GO:0055114

oxidation reduction

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR010097

P

Seeded from EcoCyc (v14.0)

complete

GO:0055114

oxidation reduction

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0560

P

Seeded from EcoCyc (v14.0)

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

Subunits of malate dehydrogenase

could be indirect

Protein

rpsD

PMID:16606699

Experiment(s):EBI-1145248

Protein

rplC

PMID:16606699

Experiment(s):EBI-1145248
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes

Cytoplasm

PMID:7028159

EchoLocation:mdh


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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MKVAVLGAAG GIGQALALLL KTQLPSGSEL SLYDIAPVTP GVAVDLSHIP TAVKIKGFSG
EDATPALEGA DVVLISAGVA RKPGMDRSDL FNVNAGIVKN LVQQVAKTCP KACIGIITNP
VNTTVAIAAE VLKKAGVYDK NKLFGVTTLD IIRSNTFVAE LKGKQPGEVE VPVIGGHSGV
TILPLLSQVP GVSFTEQEVA DLTKRIQNAG TEVVEAKAGG GSATLSMGQA AARFGLSLVR
ALQGEQGVVE CAYVEGDGQY ARFFSQPLLL GKNGVEERKS IGTLSAFEQN ALEGMLDTLK
KDIALGEEFV NK
Length

312

Mol. Wt

32.336 kDa

pI

5.6 (calculated)

Extinction coefficient

5,960 - 6,335 (calc based on 4 Y, 0 W, and 3 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Modification Site

26

phosphorylation site at S26

probability less than 75%

PMID:17938405

Modification Site

28

phosphorylation site at S28

probability less than 75%

PMID:17938405

Modification Site

193

phosphorylation site at S193

probability greater than 75%

PMID:17938405

Modification Site

280

phosphorylation site at S280

probability greater than 75%

PMID:17938405

Domain

1..145

PF00056 lactate/malate dehydrogenase, NAD binding domain

PMID:19920124

Domain

147..310

PF02866 lactate/malate dehydrogenase, alpha/beta C-terminal domain

PMID:19920124

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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=mdh taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16131126

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:947854

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0010613

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P61889

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10576

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10576

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:947854

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000569

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0571

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

Protein

E. coli K-12 MC4100

3.39E+03

molecules/cell

  • Medium: median data from rich and minimal media
  • Temperature (°C): 37
  • Doubling time (min): Growth Rate(min):
  • Growth phase: other:

emPAI

PMID:18304323

Protein

E. coli K-12 EMG2

2640

molecules/cell

  • Medium: MOPS glucose minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • Growth phase: Exponential
  • OD: OD600 = 0.2 to 0.25
  • other: aerated

Quantitative protein sequencing

Spot ID: M9

PMID: 9298646

Protein

E. coli K-12 EMG2

260

molecules/cell

  • Medium: MOPS glucose minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • Growth phase: Exponential
  • OD: OD600 = 0.2 to 0.25
  • other: aerated

Quantitative protein sequencing

Spot ID: Mlll

PMID: 9298646

Protein

E. coli K-12 MG1655

9529

molecules/cell/generation

  • Medium: MOPS Complete
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

16149

molecules/cell/generation

  • Medium: MOPS Minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

7048

molecules/cell/generation

  • Medium: MOPS Complete without Methionine
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

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Notes

Transcription and Transcriptional Regulation

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<protect>

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

mdh

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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:3382270..3382310 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

</protect>

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GEO Profiles:b3236 (EcoliWiki Page)

NCBI GEO profiles for mdh

microarray

GenExpDB:b3236 (EcoliWiki Page)

Summary of data for mdh from multiple microarray studies

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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes
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Notes

Accessions Related to mdh Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10576

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0571

Escherichia coli str. K-12 substr. MG1655

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b3236

EcoGene

EcoGene:EG10576

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000569

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0010613

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism Homologs (Statistics) Comments

Anopheles gambiae

  • ENSANGP00000020184 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Apis mellifera

  • ENSAPMP00000014039 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Arabidopsis thaliana

  • AT5G09660 (score: 1.000; bootstrap: 100%)
  • AT2G22780 (score: 0.656)
  • AT1G53240 (score: 0.242)
  • AT3G15020 (score: 0.239)
  • AT3G47520 (score: 0.199)

From Inparanoid:20070104

Bos taurus

  • ENSBTAP00000012454 (score: 1.000; bootstrap: 99%)

From Inparanoid:20070104

Caenorhabditis briggsae

  • WBGene00035534 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Caenorhabditis elegans

  • WBGene00003162 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Canis familiaris

  • ENSCAFP00000019927 (score: 1.000; bootstrap: 100%)
  • ENSCAFP00000009255 (score: 0.792)
  • ENSCAFP00000008232 (score: 0.169)

From Inparanoid:20070104

Ciona intestinalis

  • ENSCINP00000017906 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Danio rerio

  • ZDB-GENE-040426-2143 (score: 1.000; bootstrap: 100%)
  • ZDB-CDNA-040425-3090 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Dictyostelium discoideum

  • DDB0230188 (score: 1.000; bootstrap: 100%)
  • DDB0230186 (score: 1.000; bootstrap: 100%)
  • DDB0230187 (score: 0.463)

From Inparanoid:20070104

Drosophila melanogaster

  • FBgn0038587 (score: 1.000; bootstrap: 100%)
  • FBgn0036328 (score: 0.062)

From Inparanoid:20070104

Drosophila pseudoobscura

  • GA20754-PA (score: 1.000; bootstrap: 100%)
  • GA10541-PA (score: 0.071)

From Inparanoid:20070104

Gallus gallus

  • ENSGALP00000003012 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Homo sapiens

  • ENSP00000327070 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Macaca mulatta

  • ENSMMUP00000012976 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Monodelphis domestica

  • ENSMODP00000017108 (score: 1.000; bootstrap: 100%)
  • ENSMODP00000021862 (score: 0.177)

From Inparanoid:20070104

Mus musculus

  • MGI:97050 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Oryza gramene

  • Q42972 (score: 1.000; bootstrap: 100%)
  • Q7XZW5 (score: 0.685)
  • Q7XZE9 (score: 0.285)
  • Q6F361 (score: 0.285)
  • Q94JA2 (score: 0.282)
  • Q7FSL4 (score: 0.282)
  • Q8H4E0 (score: 0.207)
  • Q6YWL3 (score: 0.196)
  • Q5QLS8 (score: 0.194)

From Inparanoid:20070104

Pan troglodytes

  • ENSPTRP00000033039 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Rattus norvegicus

  • ENSRNOP00000001958 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Saccharomyces cerevisiae

  • YKL085W (score: 1.000; bootstrap: 98%)

From Inparanoid:20070104

Schizosaccharomyces pombe

  • SPCC3068c (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Takifugu rubripes

  • NEWSINFRUP00000136061 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Tetraodon nigroviridis

  • GSTENP00035367001 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Xenopus tropicalis

  • ENSXETP00000019672 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Shigella flexneri

MDH

From SHIGELLACYC

E. coli O157

MDH

From ECOO157CYC

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Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

Pfam (EcoliWiki Page)

PF02866 lactate/malate dehydrogenase, alpha/beta C-terminal domain

Pfam (EcoliWiki Page)

PF00056 lactate/malate dehydrogenase, NAD binding domain

Superfamily (EcoliWiki Page)

SUPERFAMILY:51735

Panther (EcoliWiki Page)

PTHR11540:SF26

Superfamily (EcoliWiki Page)

SUPERFAMILY:56327

EcoCyc

EcoCyc:EG10576

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10576

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000569

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0571

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0010613

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 1.8 1.9 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 2.4 2.5 2.6 2.7 2.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 3.2 3.3 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ 4.0 4.1 4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
  6. ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

Categories

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