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Standard Name	malE
Gene Synonym(s)	ECK4026, b4034, JW3994^[1], JW3994, malJ
Product Desc.	MalE^[2]^[3]; Component of maltose ABC transporter^[2]^[3]; Maltose/Maltodextrin Transport System^[3] Maltose-binding protein, periplasmic; MBP; substrate recognition for active transport of and chemotaxis toward maltose and maltodextrin^[4]
Product Synonyms(s)	maltose transporter subunit^[1], periplasmic-binding component of ABC superfamily^[1], B4034^[2]^[1], MalE^[2]^[1], MBP^[2]^[1] , ECK4026, JW3994, malJ, b4034
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): malEFG^[2], OP00047, malE
Regulation/Activity
Quick Links
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Notes

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Biofilm microarray result was validated with RT-PCR. MalE binds maltose and maltodextrin and mediates chemotaxis through the Tar chemoreceptor. MalT regulon. creBC regulon.Alkali-inducible. Upregulated in biofilms. Downregulated during cold-shock in a pnp-dependent way. Binds TrxA (Kumar, 2004).^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	malE
Mnemonic	Maltose
Synonyms	ECK4026, b4034, JW3994^[1], JW3994, malJ
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	91.46 minutes	MG1655: 4244442..4243252 <gbrowseImage> name=NC_000913:4243252..4244442 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 4225351..4224161 <gbrowseImage> name=NC_012967:4224161..4225351 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 4182097..4183287 <gbrowseImage> name=NC_012759:4182097..4183287 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 4250009..4248819 <gbrowseImage> name=NC_007779:4248819..4250009 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 4344138..4342948 <gbrowseImage> name=NC_010473:4342948..4344138 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
Coding Start (SO:0000323)	MG1655	4243255	Edman degradation	PMID:6088507 PMID:9298646 PMID:9600841 PMID:9740056 PMID:10094700
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Type	Phenotype: Description	Reference	Availability	Comments
malE (Keio:JW3994)	deletion	deletion			PMID:16738554	Shigen CGSC10875^[5]
malE52::Tn10						CGSC:7071
malE444(del)					PMID:6094487	CGSC:9077
malE57::Tn5						CGSC:9956
malE-50						CGSC:14670
malE145::Tn10						CGSC:66899
malE300::Tn10						CGSC:90746
malE730(del)::kan					PMID:16738554	CGSC:101730
malE(del)	deletion	deletion	Resistant to	Thio(5)maltose increased resistance.	PMID:6998971		Selection for mutant maltose transport system.
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW3994	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCAAAATAAAAACAGGTGCACG Primer 2:CCCTTGGTGATACGAGTCTGCG
7A4	Kohara Phage	Genobase	PMID:3038334
1F8	Kohara Phage	Genobase	PMID:3038334
malF3089::Tn10	Linked marker	CAG12164 = CGSC7476^[5]	est. P1 cotransduction: 92% ^[6]
zje-2241::Tn10	Linked marker	CAG18427 = CGSC7481^[5]	est. P1 cotransduction: 4% ^[6]
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10554	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10554	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000547	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:948538	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0549	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0013200	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	MalE
Synonyms	maltose transporter subunit^[1], periplasmic-binding component of ABC superfamily^[1], B4034^[2]^[1], MalE^[2]^[1], MBP^[2]^[1] , ECK4026, JW3994, malJ, b4034
Product description	MalE^[2]^[3]; Component of maltose ABC transporter^[2]^[3]; Maltose/Maltodextrin Transport System^[3] Maltose-binding protein, periplasmic; MBP; substrate recognition for active transport of and chemotaxis toward maltose and maltodextrin^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0048030	disaccharide binding	PMID:8399200	IPI: Inferred from Physical Interaction		F	b-cyclodextrin	Missing: with/from
	GO:0005363	maltose transmembrane transporter activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR006060	F	Seeded from EcoCyc (v14.0)	complete
	GO:0015768	maltose transport	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR006060	P	Seeded from EcoCyc (v14.0)	complete
	GO:0015768	maltose transport	PMID:2155217	IGI: Inferred from Genetic Interaction		P	Seeded from EcoCyc (v14.0)	Missing: with/from
	GO:0042597	periplasmic space	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0574	C	Seeded from EcoCyc (v14.0)	complete
	GO:0042597	periplasmic space	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-0200	C	Seeded from EcoCyc (v14.0)	complete
	GO:0042597	periplasmic space	PMID:15117946	IDA: Inferred from Direct Assay		C	Seeded from EcoCyc (v14.0)	complete
	GO:0042597	periplasmic space	PMID:2002054	IDA: Inferred from Direct Assay		C	Seeded from EcoCyc (v14.0)	complete
	GO:0048030	disaccharide binding	PMID:8399200	IPI: Inferred from Physical Interaction		F	Seeded from EcoCyc (v14.0)	Missing: with/from
	GO:0055052	ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing	PMID:2155217	IGI: Inferred from Genetic Interaction		C	Seeded from EcoCyc (v14.0)	Missing: with/from
	GO:0006974	response to DNA damage stimulus	PMID:11967071	IEP: Inferred from Expression Pattern		P		complete
	GO:0015768	maltose transport	PMID:2155217	IGI: Inferred from Genetic Interaction	EcoliWiki:lamB\|EcoliWiki:malK\|EcoliWiki:malF\|EcoliWiki:malG	P		complete
	GO:0042597	periplasmic space	PMID:2002054 PMID:15117946	IDA: Inferred from Direct Assay		C		complete
	GO:0006810	transport	PMID:2155217	IGI: Inferred from Genetic Interaction	EcoliWiki:lamB\|EcoliWiki:malK\|EcoliWiki:malF\|EcoliWiki:malG	P		complete
	GO:0008643	carbohydrate transport	PMID:2155217	IGI: Inferred from Genetic Interaction	EcoliWiki:lamB\|EcoliWiki:malK\|EcoliWiki:malF\|EcoliWiki:malG	P		complete
	GO:0015423	ATPase-coupled maltose transmembrane transporter activity	PMID:2155217	IGI: Inferred from Genetic Interaction	EcoliWiki:lamB\|EcoliWiki	F	Replaced original annotation to GO:0005215 which has been made obsolete.	complete
	GO:0055052	ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing	PMID:2155217	IGI: Inferred from Genetic Interaction	EcoliWiki:lamB\|EcoliWiki:malK\|EcoliWiki:malF\|EcoliWiki:malG	C		complete
	GO:0043190	ATP-binding cassette (ABC) transporter complex	PMID:2155217	IGI: Inferred from Genetic Interaction	EcoliWiki:lamB\|EcoliWiki:malK\|EcoliWiki:malF\|EcoliWiki:malG	C		complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes
Protein	Subunits of maltose ABC transporter	could be indirect
Protein	Subunits of Maltose/Maltodextrin Transport System	could be indirect
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Evidence	Reference/Source
Periplasm	PMID:9298646	EchoLocation:malE
periplasm		From EcoCyc^[3]
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MKIKTGARIL ALSALTTMMF SASALAKIEE GKLVIWINGD KGYNGLAEVG KKFEKDTGIK VTVEHPDKLE EKFPQVAATG DGPDIIFWAH DRFGGYAQSG LLAEITPDKA FQDKLYPFTW DAVRYNGKLI AYPIAVEALS LIYNKDLLPN PPKTWEEIPA LDKELKAKGK SALMFNLQEP YFTWPLIAAD GGYAFKYENG KYDIKDVGVD NAGAKAGLTF LVDLIKNKHM NADTDYSIAE AAFNKGETAM TINGPWAWSN IDTSKVNYGV TVLPTFKGQP SKPFVGVLSA GINAASPNKE LAKEFLENYL LTDEGLEAVN KDKPLGAVAL KSYEEELAKD PRIAATMENA QKGEIMPNIP QMSAFWYAVR TAVINAASGR QTVDEALKDA QTRITK
Length	396
Mol. Wt	43.389 kDa
pI	5.5 (calculated)
Extinction coefficient	66,350 (calc based on 15 Y, 8 W, and 0 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
motif	1-26	UniProt Manual:Signal Peptides	UniProt:P0AEX9
Domain	37..317	PF01547 Bacterial extracellular solute-binding protein		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=malE taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16131860	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:948538	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0013200	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P0AEX9	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG10554	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10554	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:948538	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000547	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0549	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Reference(s)
Protein	E. coli K-12 MC4100	9.10E+02	molecules/cell	Medium: median data from rich and minimal media Temperature (°C): 37 Doubling time (min): Growth Rate(min): Growth phase: other:	emPAI	PMID:18304323
Protein	E. coli K-12 MG1655	652	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	682	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	213	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
malEFG
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:4244422..4244462 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b4034 (EcoliWiki Page)	NCBI GEO profiles for malE
microarray	GenExpDB:b4034 (EcoliWiki Page)	Summary of data for malE from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
	GFP Fusion	Intergenic region (4243912..4244387) fused to gfpmut2.	OpenBioSystems	GFP fusion described in Zaslaver, et al. Plate:109-AZ01; Well:F12^[7]
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Notes

Accessions Related to malE Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10554	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0549	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b4034
EcoGene	EcoGene:EG10554	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000547	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0013200	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Anopheles gambiae	ENSANGP00000000875 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Shigella flexneri	MALE	From SHIGELLACYC
E. coli O157	MALE	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF01547 Bacterial extracellular solute-binding protein
Panther (EcoliWiki Page)	PTHR10424:SF1
Superfamily (EcoliWiki Page)	SUPERFAMILY:53850
EcoCyc	EcoCyc:EG10554	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10554	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000547	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0549	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0013200	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 ^3.3 ^3.4 ^3.5 ^3.6 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

malE:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to malE Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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