malE:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

malE

Mnemonic

Maltose

Synonyms

ECK4026, b4034, JW3994[1], JW3994, malJ

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Notes

Location(s) and DNA Sequence

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Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

91.46 minutes 

MG1655: 4244442..4243252
<gbrowseImage> name=NC_000913:4243252..4244442 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 4225351..4224161
<gbrowseImage> name=NC_012967:4224161..4225351 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 4182097..4183287
<gbrowseImage> name=NC_012759:4182097..4183287 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 4250009..4248819
<gbrowseImage> name=NC_007779:4248819..4250009 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 4344138..4342948
<gbrowseImage> name=NC_010473:4342948..4344138 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

4243255

Edman degradation

PMID:6088507[2]
PMID:9298646[3]
PMID:9600841[4]
PMID:9740056[5]
PMID:10094700[6]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

malE (Keio:JW3994)

deletion

deletion

PMID:16738554[7]

Shigen

CGSC10875[8]

malE52::Tn10

CGSC:7071

malE444(del)

PMID:6094487[9]

CGSC:9077

malE57::Tn5

CGSC:9956

malE-50

CGSC:14670

malE145::Tn10

CGSC:66899

malE300::Tn10

CGSC:90746

malE730(del)::kan

PMID:16738554[7]

CGSC:101730

malE(del)

deletion

deletion

Resistant to

Thio(5)maltose increased resistance.

PMID:6998971[10]

Selection for mutant maltose transport system.

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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW3994

Plasmid clone

Shigen

PMID:16769691[11]

Status:Clone OK

Primer 1:GCCAAAATAAAAACAGGTGCACG

Primer 2:CCCTTGGTGATACGAGTCTGCG

7A4

Kohara Phage

Genobase

PMID:3038334[12]

1F8

Kohara Phage

Genobase

PMID:3038334[12]

malF3089::Tn10

Linked marker

CAG12164 = CGSC7476[8]

est. P1 cotransduction: 92% [13]

zje-2241::Tn10

Linked marker

CAG18427 = CGSC7481[8]

est. P1 cotransduction: 4% [13]

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10554

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10554

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000547

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:948538

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0549

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0013200

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. Duplay, P et al. (1984) Sequences of the malE gene and of its product, the maltose-binding protein of Escherichia coli K12. J. Biol. Chem. 259 10606-13 PubMed
  3. Link, AJ et al. (1997) Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12. Electrophoresis 18 1259-313 PubMed
  4. Wilkins, MR et al. (1998) Protein identification with N and C-terminal sequence tags in proteome projects. J. Mol. Biol. 278 599-608 PubMed
  5. Tonella, L et al. (1998) '98 Escherichia coli SWISS-2DPAGE database update. Electrophoresis 19 1960-71 PubMed
  6. Blankenhorn, D et al. (1999) Acid- and base-induced proteins during aerobic and anaerobic growth of Escherichia coli revealed by two-dimensional gel electrophoresis. J. Bacteriol. 181 2209-16 PubMed
  7. 7.0 7.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  8. 8.0 8.1 8.2 CGSC: The Coli Genetics Stock Center
  9. Larson, TJ et al. (1984) sn-Glycerol-3-phosphate auxotrophy of plsB strains of Escherichia coli: evidence that a second mutation, plsX, is required. J. Bacteriol. 160 711-7 PubMed
  10. Ferenci, T (1980) Methyl-alpha-maltoside and 5-thiomaltose: analogs transported by the Escherichia coli maltose transport system. J. Bacteriol. 144 7-11 PubMed
  11. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  12. 12.0 12.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  13. 13.0 13.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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