malF:Gene
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| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Location(s) and DNA Sequence | Sequence Features | Alleles and Phenotypes | Genetic Interactions | Genetic Resources | Accessions | Links | References | Suggestions |
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Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
| Standard name |
malF |
|---|---|
| Mnemonic |
Maltose |
| Synonyms |
ECK4025, b4033, JW3993[1], JW3993, malJ |
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Notes
Location(s) and DNA Sequence
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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
|
MG1655 |
91.42 minutes, 91.42 minutes |
MG1655: 4243098..4241554 |
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NC_012967: 4224007..4222463 |
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NC_012759: 4180399..4181943 |
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W3110 |
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W3110: 4248665..4247121 |
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DH10B: 4342794..4341250 |
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Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
| Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
|---|---|---|---|---|---|
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Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
| Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
|---|---|---|---|---|---|---|---|
|
malF(del) (Keio:JW3993) |
deletion |
deletion |
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malFP420A |
P420A |
No effect |
seeded from UniProt:P02916 | ||||
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malFG380C,S |
G380C,S |
No growth on maltose |
seeded from UniProt:P02916 | ||||
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malFE401A,C,K,L |
E401A,C,K,L |
Reduction of transport rate |
seeded from UniProt:P02916 | ||||
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malFS403C,D,K,L |
S403C,D,K,L |
Reduction of transport rate |
seeded from UniProt:P02916 | ||||
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malFG407A,P |
G407A,P |
No effect |
seeded from UniProt:P02916 | ||||
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malFL334W |
L334W |
Ability to transport lactose in a saturable manner |
seeded from UniProt:P02916 | ||||
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malFL372W |
L372W |
Growth on maltose but not on media containing either maltoheptaose or maltoheptaose plus maltose |
seeded from UniProt:P02916 | ||||
|
malFN376K,H |
N376K,H |
No growth on maltose |
seeded from UniProt:P02916 | ||||
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malF3089::Tn10 |
Insertion at 4,242,342 bp in MG1655 (NC_000913) |
adapted from Nichols et al.[4] |
Synonyms: | ||||
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malF64(Oc) |
ochre (UAA) mutation | ||||||
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malF55::Tn10 |
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malF59::Tn5 |
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malF3089::Tn10 |
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malF3180::Tn10kan |
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malF729(del)::kan |
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malF(del) |
deletion |
deletion |
Resistant to |
Thio(5)maltose increase resistance. |
Selection for mutant maltose transport system | ||
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Notes
Genetic Interactions
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| Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
|---|---|---|---|---|---|---|
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Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
|
JW3993 |
Plasmid clone |
Status:Clone OK Primer 1:GCCGATGTCATTAAAAAGAAACA Primer 2:CCATCAAACTTCATTCGCGTGGC | |
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Kohara Phage |
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Kohara Phage |
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|
Linked marker |
est. P1 cotransduction: 12% [4] | ||
|
Linked marker |
est. P1 cotransduction: 3% [4] | ||
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
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Notes
Links
| Name | URL | Comments |
|---|---|---|
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ 2.0 2.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
- ↑ 3.0 3.1 3.2 3.3 CGSC: The Coli Genetics Stock Center
- ↑ 4.0 4.1 4.2 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
- ↑ 5.0 5.1 Singer, M et al. (1989) A collection of strains containing genetically linked alternating antibiotic resistance elements for genetic mapping of Escherichia coli. Microbiol. Rev. 53 1-24 PubMed
- ↑ Ferenci, T (1980) Methyl-alpha-maltoside and 5-thiomaltose: analogs transported by the Escherichia coli maltose transport system. J. Bacteriol. 144 7-11 PubMed
- ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
- ↑ 8.0 8.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
