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Standard Name	eutB
Gene Synonym(s)	ECK2436, b2441, JW2434^[1], JW2434
Product Desc.	ethanolamine ammonia-lyase, probable regulatory subunit^[2]^[3]; Component of ethanolamine ammonia-lyase^[2]^[3]
Product Synonyms(s)	ethanolamine ammonia-lyase, large subunit, heavy chain^[1], B2441^[2]^[1], EutB^[2]^[1] , ECK2436, JW2434, b2441
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): yfeG^[2]
Regulation/Activity
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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	eutB
Mnemonic	Ethanolamine utilization
Synonyms	ECK2436, b2441, JW2434^[1], JW2434
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	55.08 minutes	MG1655: 2556701..2555340 <gbrowseImage> name=NC_000913:2555340..2556701 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 2486198..2484837 <gbrowseImage> name=NC_012967:2484837..2486198 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 2441145..2442506 <gbrowseImage> name=NC_012759:2441145..2442506 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 2564125..2562764 <gbrowseImage> name=NC_007779:2562764..2564125 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 2648466..2647105 <gbrowseImage> name=NC_010473:2647105..2648466 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
Coding Start (SO:0000323)	MG1655	2555340	Edman degradation	PMID:2197274
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Reference	Availability	Comments
ΔeutB (Keio:JW2434)	deletion	deletion	PMID:16738554	Shigen CGSC9940^[4]
eutB::Tn5KAN-2 (FB20803)	Insertion at nt 578 in Plus orientation		PMID:15262929	E. coli Genome Project:FB20803	contains pKD46
ΔeutB770::kan			PMID:16738554	CGSC:100740
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW2434	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCAAACTAAAGACCACATTGTT Primer 2:CCGAAGAACAGTGACGGATCGCC
E8E3	Kohara Phage	Genobase	PMID:3038334
4E10	Kohara Phage	Genobase	PMID:3038334
nupC510::Tn10	Linked marker	CAG18468 = CGSC7410^[4]	est. P1 cotransduction: 13% ^[5]
purC80::Tn10	Linked marker	CAG18470 = CGSC7413^[4]	est. P1 cotransduction: 20% ^[5]
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG50006	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG50006	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002565	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:946924	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB4299	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0008046	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	EutB
Synonyms	ethanolamine ammonia-lyase, large subunit, heavy chain^[1], B2441^[2]^[1], EutB^[2]^[1] , ECK2436, JW2434, b2441
Product description	ethanolamine ammonia-lyase, probable regulatory subunit^[2]^[3]; Component of ethanolamine ammonia-lyase^[2]^[3]
EC number (for enzymes)	4.3.1.7^[1]
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0005737	cytoplasm				C	Seeded from Riley et al 2006 ^[1].	Missing: evidence, reference
	GO:0006520	cellular amino acid metabolic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR010628	P	Seeded from EcoCyc (v14.0)	complete
	GO:0008851	ethanolamine ammonia-lyase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR010628	F	Seeded from EcoCyc (v14.0)	complete
	GO:0008851	ethanolamine ammonia-lyase activity	GOA:spec GO_REF:0000003	IEA: Inferred from Electronic Annotation	EC:4.3.1.7	F	Seeded from EcoCyc (v14.0)	complete
	GO:0016829	lyase activity	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0456	F	Seeded from EcoCyc (v14.0)	complete
	GO:0050897	cobalt ion binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0170	F	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	Subunits of ethanolamine ammonia-lyase	could be indirect
Protein	groL		PMID:16606699	Experiment(s):EBI-1142851
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MKNANHRFFL RERLMKLKTT LFGNVYQFKD VKEVLAKANE LRSGDVLAGV AAASSQERVA AKQVLSEMTV ADIRNNPVIA YEDDCVTRLI QDDVNETAYN QIKNWSISEL REYVLSDETS VDDIAFTRKG LTSEVVAAVA KICSNADLIY GAKKMPVIKK ANTTIGIPGT FSARLQPNDT RDDVQSIAAQ IYEGLSFGVG DAVIGVNPVT DDVENLSRVL DTIYGVIDKF NIPTQGCVLA HVTTQIEAIR RGAPGGLIFQ SICGSEKGLK EFGVELAMLD EARAVGAEFN RIAGENCLYF ETGQGSALSA GANFGADQVT MEARNYGLAR HYDPFIVNTV VGFIGPEYLY NDRQIIRAGL EDHFMGKLSG ISMGCDCCYT NHADADQNLN ENLMILLATA GCNYIMGMPL GDDIMLNYQT TAFHDTATVR QLLNLRPSPE FERWLESMGI MANGRLTKRA GDPSLFF
Length	467
Mol. Wt	51.217 kDa
pI	4.9 (calculated)
Extinction coefficient	33,350 - 34,475 (calc based on 15 Y, 2 W, and 9 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	11..452	PF06751 Ethanolamine ammonia lyase large subunit (EutB)		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=eutB taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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Resource type	Source	Notes/Reference
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Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:90111437	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:946924	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0008046	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P0AEJ6	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG50006	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG50006	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:946924	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002565	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB4299	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli K-12 MC4100	1.28E+02	molecules/cell	Medium: median data from rich and minimal media Temperature (°C): 37 Doubling time (min): Growth Rate(min): Growth phase: other:	emPAI		PMID:18304323
Protein	Ecoli K-12	6.341+/-0.147	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		Single Molecule Fluorescence	PMID:20671182
mRNA	Ecoli K-12	0.037472447	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		by RNA_Seq	PMID:20671182
Protein	E. coli K-12 MG1655	175	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	101	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	171	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
yfeG
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:2556681..2556721 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b2441 (EcoliWiki Page)	NCBI GEO profiles for eutB
microarray	GenExpDB:b2441 (EcoliWiki Page)	Summary of data for eutB from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
	GFP Fusion	Intergenic region (2556669..2556803) fused to gfpmut2.	OpenBioSystems	GFP fusion described in Zaslaver, et al. Plate:109-AZ02; Well:G7^[6]
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Notes

Accessions Related to eutB Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG50006	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB4299	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b2441
EcoGene	EcoGene:EG50006	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002565	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0008046	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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<protect> See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF06751 Ethanolamine ammonia lyase large subunit (EutB)
EcoCyc	EcoCyc:EG50006	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG50006	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002565	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB4299	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0008046	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 ^1.8 ^1.9 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 ^2.7 ^2.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 ^3.3 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 CGSC: The Coli Genetics Stock Center
↑ ^5.0 ^5.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

eutB:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to eutB Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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