nupC:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

nupC

Mnemonic

Nucleoside permease

Synonyms

ECK2388, b2393, JW2389, cru[1], cru

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

54.12 minutes 

MG1655: 2511064..2512266
<gbrowseImage> name=NC_000913:2511064..2512266 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 2447267..2448469
<gbrowseImage> name=NC_012967:2447267..2448469 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 2396869..2398071
<gbrowseImage> name=NC_012759:2396869..2398071 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 2518488..2519690
<gbrowseImage> name=NC_007779:2518488..2519690 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 2602829..2604031
<gbrowseImage> name=NC_010473:2602829..2604031 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

nupC(del) (Keio:JW2389)

deletion

deletion

PMID:16738554[2]

Shigen

CGSC9909[3]

nupC510::Tn10

Insertion at 2,511,319 bp in MG1655 (NC_000913)

adapted from Nichols et al.[4]

CAG18468 = CGSC7410[3]

Synonyms:

nupC510::Tn10

CGSC:8688

nupC3146::Tn10kan

PMID:2540407[5]

CGSC:40076

nupC730(del)::kan

PMID:16738554[2]

CGSC:102164

cru +

Sensitivity to

Showdomycin sensitivty

PMID:827549[6]

nupC - nupG -

Resistant to

  • Fluorouracil resistance
  • Fluoruridine resistance
  • Fluorodeoxycytidine resistance

PMID:827549[6]

figure 1

nupC in strain S0 1145

Resistant to

Resistant to Fluorodeoxyuridine

PMID:374403[7]

S0 1145

nupC+ nupG -

Sensitivity to

Sensitivity to showdomycin

PMID:374403[7]

S0 1130

figure 5

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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW2389

Plasmid clone

Shigen

PMID:16769691[8]

Status:Clone OK

Primer 1:GCCGACCGCGTCCTTCATTTTGT

Primer 2:CCaAGCACCAGTGCTGCGATTGA

12H4

Kohara Phage

Genobase

PMID:3038334[9]

4G10

Kohara Phage

Genobase

PMID:3038334[9]

zfd-1::Tn10

Linked marker

CAG18467 = CGSC7408[3]

est. P1 cotransduction: 49% [4]
Synonyms:zfb-1::Tn10

purC80::Tn10

Linked marker

CAG18470 = CGSC7413[3]

est. P1 cotransduction: % [4]

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG11971

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG11971

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001896

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:946895

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1914

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0007893

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.0 2.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  3. 3.0 3.1 3.2 3.3 CGSC: The Coli Genetics Stock Center
  4. 4.0 4.1 4.2 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  5. Singer, M et al. (1989) A collection of strains containing genetically linked alternating antibiotic resistance elements for genetic mapping of Escherichia coli. Microbiol. Rev. 53 1-24 PubMed
  6. 6.0 6.1 Munch-Petersen, A & Mygind, B (1976) Nucleoside transport systems in Escherichia coli K12: specificity and regulation. J. Cell. Physiol. 89 551-9 PubMed
  7. 7.0 7.1 Munch-Petersen, A et al. (1979) Nucleoside transport in cells and membrane vesicles from Escherichia coli K12. J. Biol. Chem. 254 3730-7 PubMed
  8. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  9. 9.0 9.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed

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