purC:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

purC

Mnemonic

Purine

Synonyms

ECK2472, b2476, JW2461, adeg, ade(g)[1], ade

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Notes

Location(s) and DNA Sequence

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Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

55.93 minutes 

MG1655: 2595640..2594927
<gbrowseImage> name=NC_000913:2594927..2595640 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 2518446..2517733
<gbrowseImage> name=NC_012967:2517733..2518446 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 2480732..2481445
<gbrowseImage> name=NC_012759:2480732..2481445 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 2596274..2595561
<gbrowseImage> name=NC_007779:2595561..2596274 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 2687405..2686692
<gbrowseImage> name=NC_010473:2686692..2687405 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

2594927

Edman degradation

PMID:2120198[2]
PMID:9298646[3]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔpurC (Keio:JW2461)

deletion

deletion

PMID:16738554[4]

Shigen

purC::Tn5KAN-2 (FB20819)

Insertion at nt 61 in Minus orientation

PMID:15262929[5]

E. coli Genome Project:FB20819

contains pKD46

purC80::Tn10

Insertion at 2,595,621 bp in MG1655 (NC_000913)

adapted from Nichols et al.[6]

CAG18470 = CGSC7413[7]

Synonyms:

purC50

CGSC:6289

purC60

CGSC:6368

purC80::Tn10

CGSC:7116

purC3137::Tn10kan

PMID:2540407[8]

CGSC:38905


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW2461

Plasmid clone

Shigen

PMID:16769691[9]

Status:Clone OK

Primer 1:GCCCAAAAGCAAGCTGAGTTGTA

Primer 2:CCGTCCAGCTGTACACCCAGGCG

4C11

Kohara Phage

Genobase

PMID:3038334[10]

5A8

Kohara Phage

Genobase

PMID:3038334[10]

nupC510::Tn10

Linked marker

CAG18468 = CGSC7410[7]

est. P1 cotransduction: % [6]

zfh-208::Tn10

Linked marker

CAG18481 = CGSC7417[7]

est. P1 cotransduction: 2% [6]
Synonyms:zff-208::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10791

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10791

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000782

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:946957

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0784

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0008156

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ Tiedeman, AA et al. (1990) DNA sequence of the purC gene encoding 5'-phosphoribosyl-5-aminoimidazole-4-N-succinocarboxamide synthetase and organization of the dapA-purC region of Escherichia coli K-12. J. Bacteriol. 172 6035-41 PubMed
  3. ↑ Link, AJ et al. (1997) Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12. Electrophoresis 18 1259-313 PubMed
  4. ↑ Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  5. ↑ Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
  6. ↑ 6.0 6.1 6.2 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  7. ↑ 7.0 7.1 7.2 CGSC: The Coli Genetics Stock Center
  8. ↑ Singer, M et al. (1989) A collection of strains containing genetically linked alternating antibiotic resistance elements for genetic mapping of Escherichia coli. Microbiol. Rev. 53 1-24 PubMed
  9. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  10. ↑ 10.0 10.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed

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