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Standard Name	cynT
Gene Synonym(s)	ECK0336, b0339, JW0330^[1], JW0330
Product Desc.	carbonate dehydratase^[2]; carbonic anhydrase^[3]; Component of carbonic anhydrase^[2]; carbonic anhydrase 1^[3] Carbonic anhydrase, beta class^[4]
Product Synonyms(s)	carbonic anhydrase^[1], B0339^[2]^[1], CynT^[2]^[1] , ECK0336, JW0330, b0339
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): cynTSX^[2], OP00193
Regulation/Activity
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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	cynT
Mnemonic	Cyanase; cyanate metabolism
Synonyms	ECK0336, b0339, JW0330^[1], JW0330
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	7.72 minutes	MG1655: 358023..358682 <gbrowseImage> name=NC_000913:358023..358682 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 331355..332014 <gbrowseImage> name=NC_012967:331355..332014 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
W3110		W3110: 358023..358682 <gbrowseImage> name=NC_007779:358023..358682 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
Coding Start (SO:0000323)	MG1655	358023	Edman degradation	PMID:1740425
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Reference	Availability	Comments
cynT(del) (Keio:JW0330)	deletion	deletion	PMID:16738554	Shigen CGSC8525^[5]
cynT::Tn5KAN-2 (FB20161)	Insertion at nt 211 in Minus orientation		PMID:15262929	E. coli Genome Project:FB20161	does not contain pKD46
cynT1(del)				CGSC:39955
cynT780(del)::kan			PMID:16738554	CGSC:100468
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW0330	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCAAAGAGATTATTGATGGATT Primer 2:CCCGCTGCGGTCGGTTGGCGTAG
9F1	Kohara Phage	Genobase	PMID:3038334
10A6	Kohara Phage	Genobase	PMID:3038334
proA81::Tn10	Linked marker	CAG18447 = CGSC7330^[5]	est. P1 cotransduction: % ^[6]
lacI3042::Tn10	Linked marker	CAG18439 = CGSC7334^[5]	est. P1 cotransduction: 75% ^[6] Synonyms:lacI42::Tn10 nnnCAG18439 also carries lacZ118(0c) (CGSC).
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10176	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10176	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000171	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:946548	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0173	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0001171	Escherichia coli str. K-12 substr. MG1655
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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	CynT
Synonyms	carbonic anhydrase^[1], B0339^[2]^[1], CynT^[2]^[1] , ECK0336, JW0330, b0339
Product description	carbonate dehydratase^[2]; carbonic anhydrase^[3]; Component of carbonic anhydrase^[2]; carbonic anhydrase 1^[3] Carbonic anhydrase, beta class^[4]
EC number (for enzymes)	4.2.1.1^[1]
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0004089	carbonate dehydratase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001765	F	Seeded from EcoCyc (v14.0)	complete
	GO:0004089	carbonate dehydratase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR015892	F	Seeded from EcoCyc (v14.0)	complete
	GO:0004089	carbonate dehydratase activity	GOA:spec GO_REF:0000003	IEA: Inferred from Electronic Annotation	EC:4.2.1.1	F	Seeded from EcoCyc (v14.0)	complete
	GO:0008270	zinc ion binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001765	F	Seeded from EcoCyc (v14.0)	complete
	GO:0008270	zinc ion binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR015892	F	Seeded from EcoCyc (v14.0)	complete
	GO:0008270	zinc ion binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0862	F	Seeded from EcoCyc (v14.0)	complete
	GO:0015976	carbon utilization	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001765	P	Seeded from EcoCyc (v14.0)	complete
	GO:0015976	carbon utilization	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR015892	P	Seeded from EcoCyc (v14.0)	complete
	GO:0046872	metal ion binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0479	F	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	Subunits of carbonic anhydrase	could be indirect
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MKEIIDGFLK FQREAFPKRE ALFKQLATQQ SPRTLFISCS DSRLVPELVT QREPGDLFVI RNAGNIVPSY GPEPGGVSAS VEYAVAALRV SDIVICGHSN CGAMTAIASC QCMDHMPAVS HWLRYADSAR VVNEARPHSD LPSKAAAMVR ENVIAQLANL QTHPSVRLAL EEGRIALHGW VYDIESGSIA AFDGATRQFV PLAANPRVCA IPLRQPTAA
Length	219
Mol. Wt	23.763 kDa
pI	7.7 (calculated)
Extinction coefficient	16,960 - 17,710 (calc based on 4 Y, 2 W, and 6 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	34..189	PF00484 Carbonic anhydrase		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=cynT taxon=562,83333 </beststructure>

Models

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Structure figures

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Notes

Gene Product Resources

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Resource type	Source	Notes/Reference
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Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16128324	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:946548	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0001171	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P0ABE9	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG10176	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10176	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:946548	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000171	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0173	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli K-12 MG1655	24	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	14	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	18^a	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
cynTSX
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:358003..358043 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b0339 (EcoliWiki Page)	NCBI GEO profiles for cynT
microarray	GenExpDB:b0339 (EcoliWiki Page)	Summary of data for cynT from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
	GFP Fusion	Intergenic region (357826..358045) fused to gfpmut2.	OpenBioSystems	GFP fusion described in Zaslaver, et al. Plate:109-AZ20; Well:B7^[7]
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Notes

Accessions Related to cynT Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10176	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0173	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b0339
EcoGene	EcoGene:EG10176	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000171	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0001171	Escherichia coli str. K-12 substr. MG1655
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Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Anopheles gambiae	ENSANGP00000029115 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Arabidopsis thaliana	AT3G01500 (score: 1.000; bootstrap: 100%) AT5G14740 (score: 0.598) AT1G70410 (score: 0.337) AT1G23730 (score: 0.317) AT4G33580 (score: 0.113) AT1G58180 (score: 0.074)	From Inparanoid:20070104
Caenorhabditis briggsae	WBGene00035244 (score: 1.000; bootstrap: 100%) WBGene00023813 (score: 0.288)	From Inparanoid:20070104
Caenorhabditis elegans	WBGene00000245 (score: 1.000; bootstrap: 100%) WBGene00013805 (score: 0.300)	From Inparanoid:20070104
Drosophila melanogaster	FBgn0037646 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Drosophila pseudoobscura	GA11301-PA (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Oryza gramene	O80422 (score: 1.000; bootstrap: 100%) Q40628 (score: 1.000; bootstrap: 100%) Q7F2G3 (score: 1.000; bootstrap: 100%) P40880 (score: 0.718) Q41729 (score: 0.509) Q41728 (score: 0.496) Q43230 (score: 0.472) Q43231 (score: 0.441) Q69MC9 (score: 0.108)	From Inparanoid:20070104
E. coli O157	CYNT	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Panther (EcoliWiki Page)	PTHR11002:SF1
Superfamily (EcoliWiki Page)	SUPERFAMILY:53056
Pfam (EcoliWiki Page)	PF00484 Carbonic anhydrase
EcoCyc	EcoCyc:EG10176	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10176	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000171	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0173	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0001171	Escherichia coli str. K-12 substr. MG1655
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Notes

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 ^1.8 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 ^2.7 ^2.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 ^3.3 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

cynT:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to cynT Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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