tynA:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

tynA

Mnemonic

Tyramine

Synonyms

ECK1383, b1386, JW1381, feaA, maoA[1][2], maoA

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

31.19 minutes 

MG1655: 1449373..1447100
<gbrowseImage> name=NC_000913:1447100..1449373 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 1442401..1440683
<gbrowseImage> name=NC_012967:1440683..1442401 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 1339159..1341432
<gbrowseImage> name=NC_012759:1339159..1341432 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 1453063..1450790
<gbrowseImage> name=NC_007779:1450790..1453063 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 1539968..1537695
<gbrowseImage> name=NC_010473:1537695..1539968 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

1447103

Edman degradation

PMID:8631685[3]
PMID:9043126[4]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

tynAL59I

L59I

(in strain: W)

Strain variation; seeded from UniProt:P46883

tynAK42E

K42E

(in strain: W)

Strain variation; seeded from UniProt:P46883

ΔtynA (Keio:JW1381)

deletion

deletion

PMID:16738554[5]

Shigen
CGSC9221[6]

tynA::Tn5KAN-2 (FB20303)

Insertion at nt 1459 in Minus orientation

PMID:15262929[7]

E. coli Genome Project:FB20303

does not contain pKD46

tynA::Tn5KAN-2 (FB20304)

Insertion at nt 1459 in Minus orientation

PMID:15262929[7]

E. coli Genome Project:FB20304

contains pKD46

ΔtynA750::kan

PMID:16738554[5]

CGSC:103258


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW1381

Plasmid clone

Shigen

PMID:16769691[8]

Status:Clone OK

Primer 1:GCCGGAAGCCCCTCTCTGTATTC

Primer 2:CCtTTATCTTTCTTCAGCGCCCC

1E7

Kohara Phage

Genobase

PMID:3038334[9]

5F1

Kohara Phage

Genobase

PMID:3038334[9]

zhj-3076::Tn10

Linked marker

CAG18640 = CGSC7453[6]

est. P1 cotransduction: 63% [10]

trg-2::Tn10

Linked marker

CAG12026 = CGSC7376[6]

est. P1 cotransduction: 12% [10]

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG13139

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG13139

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120002427

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:945939

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB2934

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0004639

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. Yamashita, M et al. (1996) maoB, a gene that encodes a positive regulator of the monoamine oxidase gene (maoA) in Escherichia coli. J. Bacteriol. 178 2941-7 PubMed
  4. Hanlon, SP et al. (1997) 2-phenylethylamine catabolism by Escherichia coli K-12: gene organization and expression. Microbiology (Reading, Engl.) 143 ( Pt 2) 513-8 PubMed
  5. 5.0 5.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  6. 6.0 6.1 6.2 CGSC: The Coli Genetics Stock Center
  7. 7.0 7.1 Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
  8. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  9. 9.0 9.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  10. 10.0 10.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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