ompN:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

ompN

Mnemonic

Outer membrane protein

Synonyms

ECK1372, b1377, JW1371, yaaG, ynaG[1], ynaG

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

30.9 minutes 

MG1655: 1434917..1433784
<gbrowseImage> name=NC_000913:1433784..1434917 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 1428499..1427366
<gbrowseImage> name=NC_012967:1427366..1428499 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 1325843..1326976
<gbrowseImage> name=NC_012759:1325843..1326976 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 1438607..1437474
<gbrowseImage> name=NC_007779:1437474..1438607 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 1525512..1524379
<gbrowseImage> name=NC_010473:1524379..1525512 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

1433787

Edman degradation

PMID:9642192[2]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔompN (Keio:JW1371)

deletion

deletion

PMID:16738554[3]

Shigen
CGSC9213[4]

zhj-3076::Tn10

Insertion at 1,434,058 bp in MG1655 (NC_000913)

adapted from Nichols et al.[5]

CAG18640 = CGSC7453[4]

Synonyms:

ΔompN740::kan

PMID:16738554[3]

CGSC:102182


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW1371

Plasmid clone

Shigen

PMID:16769691[6]

Status:Clone OK

Primer 1:GCCAAAAGCAAAGTACTGGCACT

Primer 2:CCGAACTGATAAACCAGACCTAA

4E7

Kohara Phage

Genobase

PMID:3038334[7]

1E7

Kohara Phage

Genobase

PMID:3038334[7]

recT3061::Tn10

Linked marker

CAG12081 = CGSC7375[4]

est. P1 cotransduction: 42% [5]
Synonyms:zcj-3061::Tn10, zda-3061::Tn10

trg-2::Tn10

Linked marker

CAG12026 = CGSC7376[4]

est. P1 cotransduction: 4% [5]

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:G6700

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG13375

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120003295

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:946313

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB3157

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0004608

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. Prilipov, A et al. (1998) Identification and characterization of two quiescent porin genes, nmpC and ompN, in Escherichia coli BE. J. Bacteriol. 180 3388-92 PubMed
  3. 3.0 3.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  4. 4.0 4.1 4.2 4.3 CGSC: The Coli Genetics Stock Center
  5. 5.0 5.1 5.2 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  6. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  7. 7.0 7.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed

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