Hanlon, SP, Hill, TK, Flavell, MA, Stringfellow, JM and Cooper, RA (1997) 2-phenylethylamine catabolism by Escherichia coli K-12: gene organization and expression. Microbiology (Reading, Engl.) 143 ( Pt 2):513-8
A gene encoding phenylacetaldehyde dehydrogenase (PAD), the enzyme involved together with a copper-topaquinone-containing amine oxidase in the initial steps of 2-phenylethylamine catabolism, was located at 31.1 min on the Escherichia coli K-12 genetic map. It was immediately adjacent to the gene encoding the amine oxidase but transcribed in the opposite direction. The purified PAD acted almost equally well on phenylacetaldehyde, 4-hydroxyphenylacetaldehyde and 3,4-dihydroxyphenylacetaldehyde. It had a subunit size of 54 kDa and its deduced amino acid sequence was approximately 40% identical to various eukaryotic and prokaryotic aldehyde dehydrogenases. A third gene encoding a positive regulatory protein required for expression of the amine oxidase and PAD genes was located next to the PAD gene. A gene previously located in this position was reported to encode a second amine oxidase but this was not confirmed. The nucleotide sequence from 1447 to 1450 kb on the E. coli K-12 physical map has been determined.
Aldehyde Oxidoreductases/genetics; Aldehyde Oxidoreductases/metabolism; Amine Oxidase (Copper-Containing); Amino Acid Sequence; Escherichia coli/enzymology; Escherichia coli/genetics; Gene Expression Regulation, Bacterial; Genes, Bacterial; Genes, Regulator; Molecular Sequence Data; Oxidoreductases Acting on CH-NH Group Donors/genetics; Phenethylamines/metabolism; Restriction Mapping; Sequence Analysis, DNA; Sequence Homology, Amino Acid; Species Specificity; Substrate Specificity; Transcription, Genetic
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