tufB:On One Page

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Quickview | Gene | Product(s) | Expression| Evolution | References |

Quickview

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Standard Name

tufB

Gene Synonym(s)

ECK3971, b3980, JW3943, kirT, pulT[1], pulT

Product Desc.

elongation factor Tu[2][3]

EF-Tu, Elongation Factor-Translation, unstable; GTP-dependent binding of aa-tRNA to the A-site of ribosomes; has intrinsic GTPase activity when bound to kirromycin[4]

Product Synonyms(s)

B3980[2], PulT[2], KirT[2], TufB[2], EF-Tu[2] , ECK3971, JW3943, kirT, pulT, b3980

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): tufB[2], thrU-tyrU-glyT-thrT[2], thrU

Regulation/Activity
Quick Links

porteco.png EcoCyc.gif regulondb.jpg

dnadisplay.png proteindisplay.png   pubmed.jpg   textpresso.jpg  

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Notes

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mgiglio@SOM.UMARYLAND.EDU

tufB encodes an essential function, with duplicate gene tufA, such that tufA or tufB can be deleted, but the double mutant is inviable.[4]


Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

tufB

Mnemonic

Translation unstable factor

Synonyms

ECK3971, b3980, JW3943, kirT, pulT[1], pulT

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

89.96 minutes, 89.96 minutes 

MG1655: 4173967..4175151
<gbrowseImage> name=NC_000913:4173967..4175151 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 4155555..4156739
<gbrowseImage> name=NC_012967:4155555..4156739 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 4063647..4064831
<gbrowseImage> name=NC_012759:4063647..4064831 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 3460737..3459553
<gbrowseImage> name=NC_007779:3459553..3460737 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 4273663..4274847
<gbrowseImage> name=NC_010473:4273663..4274847 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

4173970

Edman degradation

PMID:6997043
PMID:7021545
PMID:9298646


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔtufB (Keio:JW3943)

deletion

deletion

PMID:16738554

Shigen
CGSC10844[5]

ΔtufB756::kan

PMID:16738554

CGSC:103254


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW3943

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCTCTAAAGAAAAGTTTGAACG

Primer 2:CCGCTCAGAACTTTTGCTACAAC

E11C11

Kohara Phage

Genobase

PMID:3038334

argE86::Tn10

Linked marker

CAG12185 = CGSC7471[5]

est. P1 cotransduction: 45% [6]

thiC39::Tn10

Linked marker

CAG18500 = CGSC7472[5]

est. P1 cotransduction: 48% [6]
Synonyms:thi-39::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG11037

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG11037

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001026

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:948482

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1030

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0013020

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

TufB protein chain elongation factor EF-Tu

Synonyms

B3980[2], PulT[2], KirT[2], TufB[2], EF-Tu[2] , ECK3971, JW3943, kirT, pulT, b3980

Product description

elongation factor Tu[2][3]

EF-Tu, Elongation Factor-Translation, unstable; GTP-dependent binding of aa-tRNA to the A-site of ribosomes; has intrinsic GTPase activity when bound to kirromycin[4]

EC number (for enzymes)


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Notes

Function

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<protect> Gene Ontology

elongation factor Tu

See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status


Interactions

elongation factor Tu

See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

secA

PMID:19402753

LCMS(ID Probability):99.0 MALDI(Z-score):26.150506

Protein

uspG

PMID:19402753

LCMS(ID Probability):99.0 MALDI(Z-score):22.024176

Protein

mreB

PMID:19402753

MALDI(Z-score):33.654448

Protein

dnaJ

PMID:19402753

LCMS(ID Probability):99.0 MALDI(Z-score):19.601403

Protein

tsf

PMID:19402753

LCMS(ID Probability):99.6 MALDI(Z-score):34.848841

Protein

valS

PMID:19402753

LCMS(ID Probability):99.0

Protein

metK

PMID:19402753

MALDI(Z-score):19.931900

Protein

ybeD

PMID:19402753

LCMS(ID Probability):99.0 MALDI(Z-score):17.631606

Protein

acrF

PMID:19402753

LCMS(ID Probability):99.0

Protein

yaaA

PMID:19402753

LCMS(ID Probability):99.0

Protein

ydhQ

PMID:19402753

LCMS(ID Probability):99.0

Protein

xylA

PMID:19402753

LCMS(ID Probability):99.0

Protein

afuC

PMID:19402753

LCMS(ID Probability):99.0

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes

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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc

MSKEKFERTK PHVNVGTIGH VDHGKTTLTA AITTVLAKTY GGAARAFDQI DNAPEEKARG
ITINTSHVEY DTPTRHYAHV DCPGHADYVK NMITGAAQMD GAILVVAATD GPMPQTREHI
LLGRQVGVPY IIVFLNKCDM VDDEELLELV EMEVRELLSQ YDFPGDDTPI VRGSALKALE
GDAEWEAKIL ELAGFLDSYI PEPERAIDKP FLLPIEDVFS ISGRGTVVTG RVERGIIKVG
EEVEIVGIKE TQKSTCTGVE MFRKLLDEGR AGENVGVLLR GIKREEIERG QVLAKPGTIK
PHTKFESEVY ILSKDEGGRH TPFFKGYRPQ FYFRTTDVTG TIELPEGVEM VMPGDNIKMV
VTLIHPIAMD DGLRFAIREG GRTVGAGVVA KVLS
Length

394

Mol. Wt

43.313 kDa

pI

5.3 (calculated)

Extinction coefficient

20,400 - 20,775 (calc based on 10 Y, 1 W, and 3 C residues)


Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=tufB taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16131810

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:948482

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0013020

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P0A6N1

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG11037

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG11037

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:948482

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001026

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1030

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

Protein

Ecoli K-12

2202.788+/-15.425

Molecules/cell

  • Medium:
  • Temperature (°C):
  • Doubling time (min): Growth Rate(min): *
  • Growth phase: other: *

Single Molecule Fluorescence

PMID:20671182

mRNA

Ecoli K-12

0.59955+/-0.07278

Molecules/cell

  • Medium:
  • Temperature (°C):
  • Doubling time (min): Growth Rate(min): *
  • Growth phase: other: *

by FISH

PMID:20671182

mRNA

Ecoli K-12

0.293496622

Molecules/cell

  • Medium:
  • Temperature (°C):
  • Doubling time (min): Growth Rate(min): *
  • Growth phase: other: *

by RNA_Seq

PMID:20671182

Protein

E. coli K-12 MG1655

579074

molecules/cell/generation

  • Medium: MOPS Complete
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

116583

molecules/cell/generation

  • Medium: MOPS Minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

339302

molecules/cell/generation

  • Medium: MOPS Complete without Methionine
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Notes

Expression values are unlikely to distinguish between the tufA and tufB products. See tufA:Expression

Transcription and Transcriptional Regulation

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<protect>

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

tufB

thrU-tyrU-glyT-thrT

Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:4173947..4173987 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference


Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GEO Profiles:b3980 (EcoliWiki Page)

NCBI GEO profiles for tufB

microarray

GenExpDB:b3980 (EcoliWiki Page)

Summary of data for tufB from multiple microarray studies


Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes

GFP Fusion

Intergenic region (4173320..4173572) fused to gfpmut2.

OpenBioSystems

GFP fusion described in Zaslaver, et al.
Plate:109-AZ09; Well:D6[7]

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Notes

Accessions Related to tufB Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG11037

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1030

Escherichia coli str. K-12 substr. MG1655

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b3980

EcoGene

EcoGene:EG11037

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001026

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0013020

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism Homologs (Statistics) Comments

Anopheles gambiae

  • ENSANGP00000015131 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Apis mellifera

  • ENSAPMP00000010611 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Arabidopsis thaliana

  • AT4G02930 (score: 1.000; bootstrap: 70%)

From Inparanoid:20070104

Bos taurus

  • ENSBTAP00000025586 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Caenorhabditis briggsae

  • WBGene00023870 (score: 1.000; bootstrap: 90%)

From Inparanoid:20070104

Caenorhabditis elegans

  • WBGene00007000 (score: 1.000; bootstrap: 91%)

From Inparanoid:20070104

Canis familiaris

  • ENSCAFP00000025359 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Ciona intestinalis

  • ENSCINP00000005794 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Danio rerio

  • ZDB-GENE-050320-73 (score: 1.000; bootstrap: 100%)
  • ZDB-CDNA-050320-73 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Dictyostelium discoideum

  • DDB0219464 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Drosophila melanogaster

  • FBgn0024556 (score: 1.000; bootstrap: 99%)

From Inparanoid:20070104

Drosophila pseudoobscura

  • GA19322-PA (score: 1.000; bootstrap: 99%)

From Inparanoid:20070104

Gallus gallus

  • ENSGALP00000025606 (score: 0.129)
  • ENSGALP00000022592 (score: 1.000; bootstrap: 96%)
  • ENSGALP00000009371 (score: 0.123)
  • ENSGALP00000004582 (score: 0.120)

From Inparanoid:20070104

Homo sapiens

  • ENSP00000322439 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Macaca mulatta

  • ENSMMUP00000004400 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Monodelphis domestica

  • ENSMODP00000019426 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Mus musculus

  • MGI:1923686 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Oryza gramene

  • Q6ZI53 (score: 1.000; bootstrap: 53%)
  • Q8W2C3 (score: 1.000; bootstrap: 56%)
  • Q9SEF8 (score: 0.978)

From Inparanoid:20070104

Pan troglodytes

  • ENSPTRP00000013572 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Rattus norvegicus

  • ENSRNOP00000025203 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Saccharomyces cerevisiae

  • YOR187W (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Schizosaccharomyces pombe

  • SPBC9B64c (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Takifugu rubripes

  • NEWSINFRUP00000158650 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Tetraodon nigroviridis

  • GSTENP00021887001 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Xenopus tropicalis

  • ENSXETP00000032775 (score: 1.000; bootstrap: 88%)

From Inparanoid:20070104

Shigella flexneri

TUFB

From SHIGELLACYC

E. coli O157

TUFB

From ECOO157CYC

Anopheles gambiae

  • ENSANGP00000015131 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Apis mellifera

  • ENSAPMP00000010611 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Arabidopsis thaliana

  • AT4G02930 (score: 1.000; bootstrap: 70%)

From Inparanoid:20070104

Bos taurus

  • ENSBTAP00000025586 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Caenorhabditis briggsae

  • WBGene00023870 (score: 1.000; bootstrap: 90%)

From Inparanoid:20070104

Caenorhabditis elegans

  • WBGene00007000 (score: 1.000; bootstrap: 91%)

From Inparanoid:20070104

Canis familiaris

  • ENSCAFP00000025359 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Ciona intestinalis

  • ENSCINP00000005794 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Danio rerio

  • ZDB-GENE-050320-73 (score: 1.000; bootstrap: 100%)
  • ZDB-CDNA-050320-73 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Dictyostelium discoideum

  • DDB0219464 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Drosophila melanogaster

  • FBgn0024556 (score: 1.000; bootstrap: 99%)

From Inparanoid:20070104

Drosophila pseudoobscura

  • GA19322-PA (score: 1.000; bootstrap: 99%)

From Inparanoid:20070104

Gallus gallus

  • ENSGALP00000025606 (score: 0.129)
  • ENSGALP00000022592 (score: 1.000; bootstrap: 96%)
  • ENSGALP00000009371 (score: 0.123)
  • ENSGALP00000004582 (score: 0.120)

From Inparanoid:20070104

Homo sapiens

  • ENSP00000322439 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Macaca mulatta

  • ENSMMUP00000004400 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Monodelphis domestica

  • ENSMODP00000019426 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Mus musculus

  • MGI:1923686 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Oryza gramene

  • Q6ZI53 (score: 1.000; bootstrap: 53%)
  • Q8W2C3 (score: 1.000; bootstrap: 56%)
  • Q9SEF8 (score: 0.978)

From Inparanoid:20070104

Pan troglodytes

  • ENSPTRP00000013572 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Rattus norvegicus

  • ENSRNOP00000025203 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Saccharomyces cerevisiae

  • YOR187W (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Schizosaccharomyces pombe

  • SPBC9B64c (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Takifugu rubripes

  • NEWSINFRUP00000158650 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Tetraodon nigroviridis

  • GSTENP00021887001 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Xenopus tropicalis

  • ENSXETP00000032775 (score: 1.000; bootstrap: 88%)

From Inparanoid:20070104


Do-It-Yourself Web Tools

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Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

Superfamily (EcoliWiki Page)

SUPERFAMILY:50447

Superfamily (EcoliWiki Page)

SUPERFAMILY:50465

Superfamily (EcoliWiki Page)

SUPERFAMILY:52540

EcoCyc

EcoCyc:EG11037

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG11037

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001026

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1030

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0013020

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. 1.0 1.1 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 2.13 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. 3.0 3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. 4.0 4.1 4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
  6. 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  7. Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

Categories

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