tufA:Expression

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Overview Transcription Translation Turnover Experimental Links References Suggestions

Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

Protein

Ecoli K-12

3350.186+/-21.275

Molecules/cell

  • Medium:
  • Temperature (°C):
  • Doubling time (min): Growth Rate(min): *
  • Growth phase: other: *

Single Molecule Fluorescence

PMID:20671182[1]

mRNA

Ecoli K-12

1.7209+/-0.04889

Molecules/cell

  • Medium:
  • Temperature (°C):
  • Doubling time (min): Growth Rate(min): *
  • Growth phase: other: *

by FISH

PMID:20671182[1]

mRNA

Ecoli K-12

0.443412162

Molecules/cell

  • Medium:
  • Temperature (°C):
  • Doubling time (min): Growth Rate(min): *
  • Growth phase: other: *

by RNA_Seq

PMID:20671182[1]

Protein

5.1

% total protein

  • Medium: M9
  • Temperature (°C):
  • Doubling time (min): Growth Rate(min): 52.2

purification

E. coli BE

PMID:775340[2]

Protein

4.6

% total protein

  • Medium: M9+casamino acids
  • Temperature (°C):
  • Doubling time (min): Growth Rate(min): 28.8

purification

E. coli BE

PMID:775340[2]

Protein

4.5

% total protein

  • Medium: Tryptone
  • Temperature (°C):
  • Doubling time (min): Growth Rate(min): 28.8

purification

E. coli BE

PMID:775340[2]

Protein

2.7

% total protein

  • Medium: Acetate
  • Temperature (°C):
  • Doubling time (min): Growth Rate(min): 270

purification

E. coli BE

PMID:775340[2]

Protein

70000

Molecules/cell

  • Medium:

purification

PMID:775340[2] cites PMID:77620[3] for the number

PMID:775340[2]

Protein

E. coli K-12 MG1655

579074

molecules/cell/generation

  • Medium: MOPS Complete
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID:24766808[5]

Protein

E. coli K-12 MG1655

116583

molecules/cell/generation

  • Medium: MOPS Minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID:24766808[5]

Protein

E. coli K-12 MG1655

339302

molecules/cell/generation

  • Medium: MOPS Complete without Methionine
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID:24766808[5]

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Notes

Values are unlikely to distinguish between products from the tufA and tufB genes.

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

fusA-tufA

rpsLG-fusA-tufA

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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:3469331..3469371 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GEO Profiles:b3339 (EcoliWiki Page)

NCBI GEO profiles for tufA

microarray

GenExpDB:b3339 (EcoliWiki Page)

Summary of data for tufA from multiple microarray studies

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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes

GFP Fusion

Intergenic region (3468870..3469072) fused to gfpmut2.

OpenBioSystems

GFP fusion described in Zaslaver, et al.
Plate:109-AZ09; Well:B1[6]

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Notes

Accessions Related to tufA Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG11036

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1029

Escherichia coli str. K-12 substr. MG1655

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b3339

EcoGene

EcoGene:EG11036

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001025

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0010912

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 Taniguchi, Y et al. (2010) Quantifying E. coli proteome and transcriptome with single-molecule sensitivity in single cells. Science 329 533-8 PubMed
  2. ↑ 2.0 2.1 2.2 2.3 2.4 2.5 Jacobson, GR & Rosenbusch, JP (1976) Abundance and membrane association of elongation factor Tu in E. coli. Nature 261 23-6 PubMed
  3. ↑ Madzharov, M (1977) Total corrective rhinoplasty by rhinomegaly and side-deviation of nose performed in one step. Acta Chir Plast 19 201-8 PubMed
  4. ↑ Jacobson, GR et al. (1976) Properties of a major protein released from Escherichia coli by osmotic shock. Biochemistry 15 2297-303 PubMed
  5. ↑ 5.0 5.1 5.2 Li, GW et al. (2014) Quantifying absolute protein synthesis rates reveals principles underlying allocation of cellular resources. Cell 157 624-35 PubMed
  6. ↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

Categories

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